Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

Treatment Of Chromium Pollution By The Reductase Enzyme Generated By A Chromium-Resistant Bacterium Isolated In Dewatering Sludge

Paul Fabrice Nguema, Zejiao Luo, Zachari Mohamadou Mounir, Ma Jun

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
An efficient Cr (VI)-resistant and reducing bacterial strain was successfully isolated in dewatering sludge collected from a sewage treatment plant, and characterized in vitro Cr (VI) reduction through a reductase enzyme. Phylogenetic analysis using 16S rDNA gene sequencing revealed that the newly isolated strain namely Pf-1 was closely related to Bacillus cereus. The strain almost reduced 0.17 mM Cr (VI) within 24h incubation and the presence of different substrates such as glucose, sucrose, or acetate significantly enhanced the reduction rate of Cr (VI) to Cr (III). However, addition of the same substrate at the stationary phase of the microbial growth increased the reduction rate as well as bacterial growth. Additionally, raising the concentration of thiosulfate in the medium doubled the reduction rate under similar conditions. Assay with different fractions of the cells demonstrated that the reductase enzyme activity was mainly associated with the cytoplasmic fraction. The maximum activity was 23.3 µM h-1 mg-1 protein and was obtained at the Cr (VI) concentration of 2 mM. The promising strain can be efficiently employed for bioremediation of Cr (VI) polluted sites.