Online citations, reference lists, and bibliographies.
← Back to Search

An Efficient Protocol For In Vitro Organogenesis And Antioxidant Studies In Melia Dubia Cav

S. N. Shah, Tareq A. Wani, B. Ram, M. Koul, P. Awasthi, D. S. Rajput, G. R. S. Reddy
Published 2016 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Melia dubia Cav. (Meliaceae) is a multipurpose tree of tropical and subtropical regions mainly cultivated for its medicinal and industrial importance. Due to its versatile properties, it has been depleted in its natural environment. Moreover due to sluggish and poor seed germination, there is a threat of its gene pool exclusion from the natural habitat. The alternative method for conservation and efficient mass propagation is thus need of the hour. As per the extensive literature survey there is no report on efficient protocol for mass propagation of M. dubia through callus organogenesis . Therefore, the present work was aimed to develop in vitro organogenesis protocol for rapid and large scale production of planting material. From our results, maximum callus percentage, callus weight and fragile callus was observed on 1.0 mg/l benzylaminopurine (BAP) in combination with 0.5 mg/l naphthalene acetic acid (NAA). The callus differentiation was achieved at different concentrations of BAP and indole acetic acid (IAA). Multiple Shoot number per callus propagule 5.30 was observed on 0.5 mg/l BAP and 1 mg/l IAA concentration. The maximum rooting percentage (78.5%), root number per explant (4.33) and root length per explant (4.41 cm) was observed at 0.5 mg/l indol butyric acid (IBA) after 30 days of inoculation. Further the total flavonoid content, phenolic content and antioxidant properties of leaves of in-vitro regenerated plants where studied. Total flavonoids and phenolic content in leaves of in vitro Melia dubia was 0.56 ± 0.8 mg quercitin equivalent (QE) and 2.97 ± 0.17 mg gallic acid equivalent (GAE) respectively. The antioxidant property was further assed through measurement of DPPH radical scavenging activity. The in-vitro regeneration protocol can be exploited for commercial cultivation and fulfilling the growing demand for fresh explant material through mass propagation of M. dubia an economically important plant species. Key words : Melia dubia, antioxidant, indole-3-butyric acid, flavonoids and phenolics.
This paper references
Indian Medicinal Plants
B. Evers (1875)
10.1038/1811199A0
Antioxidant Determinations by the Use of a Stable Free Radical
M. S. Blois (1958)
10.1111/J.1399-3054.1962.TB08052.X
A revised medium for rapid growth and bio assays with tobacco tissue cultures
T. Murashige (1962)
10.1007/978-1-4020-5005-3
Plant Propagation by Tissue Culture
E. F. George (1984)
10.1016/0304-4238(84)90143-2
In vitro propagation of some olive (Olea europaea sativa L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos
E. Rugini (1984)
Tissue culture of forest trees in India
A. F. Mascarenhas (1989)
Somatic embryogenesis and organogenesis in Neem ( Azadirachta indica A . Juss . )
R Islam (1993)
Plant propagation by tissue culture, Part I: The Technology Exegetics Ltd
E F George (1993)
Somatic embryogenesis and organogenesis in Neem (Azadirachta indica A
R Islam (1993)
Life Sci. 1:59-63
J. Tree (1996)
Utilization of tissue culture technique for propagation of Melia azedarach
S Chaicharoen (1996)
10.1007/978-94-011-5750-6_22
The production of haploids
R. L. M. Pierik (1997)
In vitro multiplication of Gymnema sylvestre R.Br. – An important medicinal plant
S. P. Reddy (1998)
In vitro plant regeneration of Melia azaderach L . Plant Cell Rep
R Thakur (1998)
In vitro plant regeneration of Melia azaderach L
R Thakur (1998)
Regeneration of plants via adventitious bud formation from mature zygotic embryo axis of tamarind (Tamarindus indica L.
Urmil J. Mehta (2000)
Plant Tissue Culture: Techniques and Experiments
R. Smith (2000)
10.1007/s00299-002-0565-6
Production of haploids of neem (Azadirachta indica A. Juss.) by anther culture
R. Chaturvedi (2002)
10.1021/JF011178N
Polyphenolic antioxidants from the fruits of Chrysophyllum cainito L. (Star Apple).
X. Luo (2002)
10.1021/NP020216+
Antineoplastic agents. 489. Isolation and structures of meliastatins 1-5 and related euphane triterpenes from the tree Melia dubia
G. Pettit (2002)
Polyphenolic antioxidants
XD Luo (2002)
Callus induction and plantlet regeneration in Bixa orellana L
PS Sha Valli Khan (2002)
10.1002/PTR.1147
In vitro antimicrobial efficacy of leaf essential oils of Chukrasia tabularis Adr. Juss. and Melia dubia Cav. (Meliaceae)
M. Nagalakshmi (2003)
Wood anatomy and fibre quality of utap-utap (Aromadendron elegans BI.) and seven other lesser known wood species
YI Mandang (2003)
In vitro antimicrobial efficacy of leaf essential oils of Chukrasia tabularis Adr
MAH Nagalakshmi (2003)
10.1023/A:1006421228598
In vitro micropropagation of Gymnema sylvestre – A multipurpose medicinal plant
N. Komalavalli (2004)
Antiviral activity of medicinal plants of Nilgiris.
P. Vijayan (2004)
10.1007/BF00232266
In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation
Sanjay Saxena (2004)
10.1016/J.FOODCHEM.2003.06.020
Extraction of antioxidant phenolics from almond hulls (Prunus amygdalus) and pine sawdust (Pinus pinaster)
Manuel Pinelo (2004)
The resistance of five wood species against several wood destroying fungi
SD Suprapti (2004)
10.5962/bhl.title.137025
Indian Medicinal Plants
K. R. Kirtikar (2005)
10.1016/J.SCIENTA.2005.05.014
Micropropagation of Vitis thunbergii Sieb. et Zucc., a medicinal herb, through high-frequency shoot tip culture
Mei-Chun Lu (2005)
Plantation technology for selected indigenous trees in the Indian peninsula
K. Nair (2005)
10.2225/VOL9-ISSUE3-FULLTEXT-13
An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
S. Sharry (2006)
An alternative pathway for plant in vitro rgeneration of chinaberry – tree Melia azedarach L
S Sharry (2006)
10.1079/IVP2001284
Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding tree
P. S. Sha Valli Khan (2007)
10.1079/IVP2002377
Somatic embryogenesis and plant regeneration from immature zygotic embryos of Melia azedarach (Meliaceae)
S. Vila (2007)
Antimicrobial activity of Melia dubia leaf volatile oil and camphene compound against skin pathogens
SJ Gerige (2007)
Clonal propagation of Melia dubia (Cav)
KB Manjunatha (2007)
10.3923/AJPS.2008.660.665
In vitro Propagation of Gymnema sylvestre
C. S. Devi (2008)
10.1007/BF02894638
Plant Tissue Culture: Theory and Practice
V. Žárský (2008)
Evaluation of hypoglycaemic and antidiabetic effect of Melia dubia CAV fruits in mice
T. Susheela (2008)
10.1016/J.SAJB.2008.08.005
The effect of medium, carbon source and explant on regeneration and control of shoot-tip necrosis in Harpagophytum procumbens.
N. Jain (2009)
Integrating Melia dubia in agroforestry farms as an alternate pulpwood species.
K. Parthiban (2009)
10.1007/s10535-009-0067-2
In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica
P. Srivastava (2009)
Medicinal plants of sacred groves in Kanyakumari district Southern Western Ghats
S. Sukumaran (2010)
PIVOTAL ROLE OF PLANT GROWTH REGULATORS IN CLONAL PROPOGATION OF MELIA AZEDARACH L. Research Article
A. Sen (2010)
10.1186/1753-6561-5-S7-P131
Development of micro-propagation and mini cutting protocol for fast growing Melia, Dalbergia and Eucalyptus clones for pulpwood and bio-energy plantations
Senniappan Chinnaraj (2011)
A Review on Fruits Having Anti-Diabetic Potential
Rohini Sharma (2011)
Effects of Pre-sowing Seed Treatments on Germination and Seedling Growth Performance of Melia dubia CAV: An Important Multipurpose Tree.
B. Anand (2012)
10.1016/J.FOODCHEM.2011.07.127
Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results
K. Mishra (2012)
10.7897/2277-4343.04322
PRELIMINARY PHYTOCHEMICAL ANALYSIS AND BIOLOGICAL SCREENING OF EXTRACTS OF LEAVES OF MELIA DUBIA CAV
P. Valentina (2013)
Phytochemical characterization of Melia dubia for their biological properties
S. Murugesan (2013)
Photochemical characterization of Melia dubia for their biological properties
S Murugesan (2013)
An efficient protocol for micropropagation and genetic stability analysis of Melia dubia Cav. - an important multipurpose tree.
B. Ram (2014)
10.1016/J.BJBAS.2015.05.001
Studies on total phenolics, total flavonoids and antimicrobial activity from the leaves crude extracts of neem traditionally used for the treatment of cough and nausea
Hoda Salim Khamis Al-Jadidi (2015)
A CONCISE REVIEW ON MELIA DUBIA CAV. (MELIACEAE)
Gopal (2015)
Studies on total phenolics , total flavonoids and antimicrobial activity from leaves crude extracts of neem for the treatment of cough and nausea . Ben - Suef Univ J Basic Appl . Sci
HS KhamisAl-Jadidi (2015)
Studies on total phenolics, total flavonoids and antimicrobial activity from leaves crude extracts of neem for the treatment of cough and nausea
Khamis Al-Jadidi (2015)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar