Online citations, reference lists, and bibliographies.
← Back to Search

Cholesterol Derivatives Based Charged Liposomes For Doxorubicin Delivery: Preparation, In Vitro And In Vivo Characterization

Yu Nie, L. Ji, H. Ding, L. Xie, L. Li, B. He, Y. Wu, Z. Gu
Published 2012 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Cholesterol plays a critical role in liposome composition. It has great impact on the behavior of liposome in vitro and in vivo. In order to verify the possible effects from cholesterol charge, surface shielding and chemical nature, two catalogs of liposomes with charged and PEGylated cholesterols were synthesized. Anionic liposomes (AL) and cationic liposomes (CL) were prepared, with charges from hemisuccinate and lysine in cholesterol derivatives, respectively. Characteristics of different formulated liposomes were investigated after doxorubicin encapsulation, using neutral liposomes (NL) as control. Results showed that after PEGylation, AL and CL liposomes displayed prolonged retention release profile, while kept similar size distribution, encapsulation efficiency, low cytotoxicity and hemolysis comparing with NL. Confocal laser scanning microscopy and flow cytometry experiments confirmed the significantly higher cell uptake from AL and CL vesicles than the NL in mouse breast carcinoma and melanoma cells, human epithelial carcinoma and hepatoma cells. It was in accordance with our corresponding cellular mortality studies of DOX-loaded liposomes. The in vivo anti-tumor effect experiments from charged liposomes also presented much higher tumor inhibition effect (70% vs 45%, p < 0.05) than NL liposomes. This is the first time reporting anti-cancer effect from charged cholesterol liposome with/without PEGylation. It may give deeper understanding on the liposome formulation which is critical for liposome associated drug research and development.
This paper references
Addressing the problem of cationic lipid-mediated toxicity: the magnetoliposome model.
S. Soenen (2009)
Gold Nanorods Coated with Multilayer Polyelectrolyte as Contrast Agents for Multimodal Imaging
Hong Ding (2007)
Uptake of liposomes by cultured mouse bone marrow macrophages: influence of liposome composition and size.
T. Allen (1991)
Quantitative analysis of liposome-cell interactions in vitro: rate constants of binding and endocytosis with suspension and adherent J774 cells and human monocytes.
K. Lee (1993)
Surface-modified biodegradable albumin nano- and microspheres. II: effect of surface charges on in vitro phagocytosis and biodistribution in rats.
M. Roser (1998)
Co-delivery of siRNA and an anticancer drug for treatment of multidrug-resistant cancer.
M. Saad (2008)
Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts
M. Lundqvist (2008)
Direct comparison of two pegylated liposomal doxorubicin formulations: is AUC predictive for toxicity and efficacy?
Jingxia Cui (2007)
Liposomal pegylated doxorubicin plus vinorelbine combination as first-line chemotherapy for metastatic breast cancer in elderly women ≥65 years of age
R. Addeo (2007)
Polyethylene glycol-complexed cationic liposome for enhanced cellular uptake and anticancer activity.
S. Jung (2009)
Preparation and gene delivery of alkaline amino acids-based cationic liposomes
L. Li (2008)
Liposome-cell interactions in vitro: effect of liposome surface charge on the binding and endocytosis of conventional and sterically stabilized liposomes.
C. R. Miller (1998)
Liposome based systems for systemic siRNA delivery: stability in blood sets the requirements for optimal carrier design.
K. Buyens (2012)
Cholesteryl hemisuccinate exhibits pH sensitive polymorphic phase behavior.
I. M. Hafez (2000)
Influence of the formulation on the tolerance profile of nanoparticle-bound doxorubicin in healthy rats: focus on cardio- and testicular toxicity.
E. Pereverzeva (2007)
Pharmacokinetics and biodistribution studies of Doxorubicin loaded poly(butyl cyanoacrylate) nanoparticles synthesized by two different techniques.
L. H. Reddy (2004)
Surface charged temoporfin-loaded flexible vesicles: in vitro skin penetration studies and stability.
Nina Dragicevic-Curic (2010)
Effect of methimazole treatment on doxorubicin-induced cardiotoxicity in mice.
A. M. Osman (2009)
PEGylation of a Factor VIII–Phosphatidylinositol Complex: Pharmacokinetics and Immunogenicity in Hemophilia A Mice
Aaron Peng (2011)
New chimeric advanced Drug Delivery nano Systems (chi-aDDnSs) as doxorubicin carriers.
K. Gardikis (2010)
Intravenous tolerance of a nanoparticle-based formulation of doxorubicin in healthy rats.
E. Pereverzeva (2008)
Cholesterol-based anionic long-circulating cisplatin liposomes with reduced renal toxicity.
Y. Kuang (2012)
Tumor-targeted liposomes: doxorubicin-loaded long-circulating liposomes modified with anti-cancer antibody.
A. Lukyanov (2004)
Evaluation of blood compatibility of MeO‐PEG‐poly (D,L‐lactic‐co‐glycolic acid)‐PEG‐OMe triblock copolymer
Yourong Duan (2006)
Comparative study on liposome targeting to tumor endothelium.
K. Park (2012)
Lipid- and Polymer-Based Nanostructures for Cancer Theranostics
Brian T. Luk (2012)
Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating.
T. Levchenko (2002)
Protective effect of PEGylation against poly(amidoamine) dendrimer-induced hemolysis of human red blood cells.
W. Wang (2010)
Effects of particle size and surface charge on cellular uptake and biodistribution of polymeric nanoparticles.
Chunbai He (2010)
Recognition of liposomes by cells: in vitro binding and endocytosis mediated by specific lipid headgroups and surface charge density.
K. Lee (1992)
Oxaliplatin encapsulated in PEG-coated cationic liposomes induces significant tumor growth suppression via a dual-targeting approach in a murine solid tumor model.
Amr S Abu Lila (2009)
Noninvasive real-time fluorescence imaging of the lymphatic uptake of BSA-IRDye 680 conjugate administered subcutaneously in mice.
F. Wu (2012)
Liposomes and inorganic nanoparticles for drug delivery and cancer imaging.
C. Heneweer (2012)
The effect of surface charge on in vivo biodistribution of PEG-oligocholic acid based micellar nanoparticles.
K. Xiao (2011)
Ammonium Sulfate Gradients for Efficient and Stable Remote Loading of Amphipathic Weak Bases into Liposomes and Ligandoliposomes.
E. Bolotin (1994)
Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome–DNA complexes
V. Torchilin (2003)
Effect of liposome charge and PEG polymer layer thickness on cell-liposome electrostatic interactions.
N. Dan (2002)
Modulation of murine liver macrophage clearance of liposomes by diethylstilbestrol. The effect of vesicle surface charge and a role for the complement receptor Mac-1 (CD11b/CD18) of newly recruited macrophages in liposome recognition.
S. Moghimi (2002)
Nanotheranostics for personalized medicine.
S. Mura (2012)
Image Guided Biodistribution and Pharmacokinetic Studies of Theranostics
H. Ding (2012)
Esterase-catalyzed dePEGylation of pH-sensitive vesicles modified with cleavable PEG-lipid derivatives.
Huan Xu (2008)
Radiolabeled Cyclic RGD Peptides as Radiotracers for Imaging Tumors and Thrombosis by SPECT
Y. Zhou (2011)
Bioconjugated PLGA-4-arm-PEG branched polymeric nanoparticles as novel tumor targeting carriers.
Hong Ding (2011)
Electrostatically mediated liposome fusion and lipid exchange with a nanoparticle-supported bilayer for control of surface charge, drug containment, and delivery.
J. Liu (2009)
Influence of PAMAM dendrimers on human red blood cells.
D. Domański (2004)
Transmembrane ammonium sulfate gradients in liposomes produce efficient and stable entrapment of amphipathic weak bases.
G. Haran (1993)
Vascular targeting of doxorubicin using cationic liposomes.
J. Wu (2007)
Peritoneal retention of liposomes: Effects of lipid composition, PEG coating and liposome charge.
S. Dadashzadeh (2010)

This paper is referenced by
Physical properties and biological interactions of liposomes developed as a drug carrier in the field of regenerative medicine
S. Lewicki (2017)
Nanotheranostics ˗ Application and Further Development of Nanomedicine Strategies for Advanced Theranostics
M. Muthu (2014)
Injectable liposomal formulations of opiorphin as a new therapeutic strategy in pain management
N. Mennini (2015)
Multifunctional Liposome Nanocarriers Combining Upconverting Nanoparticles and Anticancer Drugs.
Yue Huang (2016)
Preparation and evaluation of PCL-PEG-PCL polymeric nanoparticles for doxorubicin delivery against breast cancer
L. Zhang (2016)
Mono-arginine Cholesterol-based Small Lipid Nanoparticles as a Systemic siRNA Delivery Platform for Effective Cancer Therapy
Jinju Lee (2016)
Enhanced blood-brain barrier transmigration using a novel transferrin embedded fluorescent magneto-liposome nanoformulation.
H. Ding (2014)
Formulation and advantages of furazolidone in spray dried and liposomal drug delivery systems
Muhammad Irfan Alam (2017)
Biochemical characterization of the interactions between doxorubicin and lipidic GM1 micelles with or without paclitaxel loading
V. Leonhard (2015)
Nature of the charged headgroup determines the fusogenic potential and membrane properties of lithocholic acid phospholipids.
Priyanshu Bhargava (2014)
Transferrin and octaarginine modified dual-functional liposomes with improved cancer cell targeting and enhanced intracellular delivery for the treatment of ovarian cancer
Pranali P. Deshpande (2018)
Image Guided Biodistribution of Drugs and Drug Delivery
H. Ding (2012)
Liposomal chemotherapeutics.
E. Gentile (2013)
Hyaluronic acid derivative-coated nanohybrid liposomes for cancer imaging and drug delivery.
Ju-Hwan Park (2014)
Liposomal formulations of carboplatin injected by convection-enhanced delivery increases the median survival time of F98 glioma bearing rats
Minghan Shi (2018)
Preparation and optimisation of anionic liposomes for delivery of small peptides and cDNA to human corneal epithelial cells
Luís F F Neves (2016)
Based Analysis of Factors Affecting Cellular Uptake in Nanoparticle Drug System
J. Yu (2017)
Synthesis, antimicrobial and cytotoxicity evaluation of new cholesterol congeners
M. R. Aly (2015)
pH-sensitive stearoyl-PEG-poly(methacryloyl sulfadimethoxine) decorated liposomes for the delivery of gemcitabine to cancer cells.
Sara Bersani (2014)
Image-guided drug delivery to the brain using nanotechnology.
Hong Ding (2013)
Nanolipolee-007, a Novel Nanoparticle-Based Drug Containing Leelamine for the Treatment of Melanoma
R. Gowda (2014)
Design and evaluation of pH-sensitive liposomes constructed by poly(2-ethyl-2-oxazoline)-cholesterol hemisuccinate for doxorubicin delivery.
H. Xu (2015)
A pH-sensitive stearoyl-PEG-poly(methacryloyl sulfadimethoxine)-decorated liposome system for protein delivery: An application for bladder cancer treatment.
Marian Vila-Caballer (2016)
Editorial Image Guided Biodistribution of Drugs and Drug Delivery
Hong Qin Ding (2017)
The comparative effects of acetylated and deacetylated galactose derivatives in liposomal gene delivery.
S. R. Mokhosi (2014)
Cell Membrane Capsules for Encapsulation of Chemotherapeutic and Cancer Cell Targeting in Vivo.
L. Peng (2015)
A novel strategy inducing autophagic cell death in Burkitt's lymphoma cells with anti-CD19-targeted liposomal rapamycin
K. Ono (2014)
Transferrin Modified Nano-liposome Co-delivery Strategies for Enhancing the Cancer Therapy.
Jia Fu (2019)
Enhancement of Skin Penetration of Hydrophilic and Lipophilic Compounds by pH-sensitive Liposomes.
Y. Tokudome (2015)
Noninvasive visualization of early osteoarthritic cartilage using targeted nanosomes in a destabilization of the medial meniscus mouse model
H. Cho (2018)
Targeting the Atypical Chemokine Receptor ACKR3/CXCR7 for the treatment of cancer and other diseases
R. D. Vestal (2015)
Materials promoting viral gene delivery.
Kübra Kaygisiz (2020)
See more
Semantic Scholar Logo Some data provided by SemanticScholar