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Investigation Of Fe(III) Reduction In Geobacter Sulfurreducens: Characterization Of Outer Surface Associated Electron Transfer Components.

Xinlei Qian
Published 2009 · Chemistry

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The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by G. sulfurreducens were previously predicted to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits interaction with Fe(III). Treatment of whole cells with proteinase K inhibited Fe(III) reduction, but had no impact on inner membrane-associated fumarate reduction. OmcB was digested by the protease, resulting in a smaller peptide. However, immunogold labeling coupled with transmission electron microscopy (TEM) did not detect OmcB, suggesting that is only partially exposed on the cell surface. In contrast, OmpB was completely digested by the protease. OmpB was loosely associated with the cell surface, as a substantial portion of it was recovered in the culture supernatant. Immunogold labeling demonstrated that OmpB associated with the cell was evenly distributed on the cell surface rather than localized to one side of the cell like the conductive pili. Although several proteins required for Fe(III) oxide reduction are shown to be exposed on the outer surface of G. sulfurreducens, the finding that OmcB is also surface-exposed is the first report that a protein required for optimal Fe(III) citrate reduction is at least partially accessible on the cell surface.
This paper references
10.1128/AEM.62.4.1458-1460.1996
Anaerobic, nitrate-dependent microbial oxidation of ferrous iron.
K. L. Straub (1996)
10.1128/AEM.68.5.2294-2299.2002
Mechanisms for Accessing Insoluble Fe(III) Oxide during Dissimilatory Fe(III) Reduction by Geothrix fermentans
K. Nevin (2002)
10.1128/AEM.60.10.3752-3759.1994
Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism.
F. Caccavo (1994)
10.1128/AEM.69.3.1548-1555.2003
Electricity Production by Geobacter sulfurreducens Attached to Electrodes
D. Bond (2003)
10.1016/J.GCA.2008.04.035
Low-oxygen and chemical kinetic constraints on the geochemical niche of neutrophilic iron(II) oxidizing microorganisms
G. Druschel (2008)
10.1038/382445A0
Humic substances as electron acceptors for microbial respiration
D. Lovley (1996)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1016/S0958-1669(00)00207-X
Microbial detoxification of metals and radionuclides.
J. Lloyd (2001)
10.1128/AEM.66.9.3743-3749.2000
Direct and Fe(II)-Mediated Reduction of Technetium by Fe(III)-Reducing Bacteria
J. Lloyd (2000)
10.1128/AEM.71.10.6308-6318.2005
Microbiological and Geochemical Heterogeneity in an In Situ Uranium Bioremediation Field Site
H. Vrionis (2005)
10.1111/j.1574-6968.2008.01252.x
Investigation of direct vs. indirect involvement of the c-type cytochrome MacA in Fe(III) reduction by Geobacter sulfurreducens.
Byoung-Chan Kim (2008)
10.1128/AEM.68.5.2300-2306.2002
Enrichment of Members of the Family Geobacteraceae Associated with Stimulation of Dissimilatory Metal Reduction in Uranium-Contaminated Aquifer Sediments
D. Holmes (2002)
10.1128/AEM.67.10.4619-4629.2001
Relationships between Microbial Community Structure and Hydrochemistry in a Landfill Leachate-Polluted Aquifer
W. Röling (2001)
10.1016/0076-6879(94)35143-0
Isolation of outer membranes.
H. Nikaido (1994)
Extracellular electron transfer
M. Hernandeza
10.1128/AEM.70.5.3091-3095.2004
Vanadium Respiration by Geobacter metallireducens: Novel Strategy for In Situ Removal of Vanadium from Groundwater
I. Ortiz-Bernad (2004)
10.1128/AEM.71.12.8634-8641.2005
Outer Membrane c-Type Cytochromes Required for Fe(III) and Mn(IV) Oxide Reduction in Geobacter sulfurreducens
T. Mehta (2005)
10.1128/AEM.67.7.3180-3187.2001
Development of a Genetic System forGeobacter sulfurreducens
M. Coppi (2001)
10.1146/ANNUREV.NEURO.24.1.1
PDZ domains and the organization of supramolecular complexes.
M. Sheng (2001)
10.1016/S0005-2736(97)00034-5
Outer membrane cytochromes of Shewanella putrefaciens MR-1: spectral analysis, and purification of the 83-kDa c-type cytochrome.
C. Myers (1997)
10.1021/ES051218U
Microbial incorporation of 13C-labeled acetate at the field scale: detection of microbes responsible for reduction of U(VI).
Y. Chang (2005)
10.1128/AEM.71.10.5983-5991.2005
Geobacteraceae Community Composition Is Related to Hydrochemistry and Biodegradation in an Iron-Reducing Aquifer Polluted by a Neighboring Landfill
B. Lin (2005)
10.1039/B816647A
Cyclic voltammetry of biofilms of wild type and mutant Geobacter sulfurreducens on fuel cell anodes indicates possible roles of OmcB, OmcZ, type IV pili, and protons in extracellular electron transfer
H. Richter (2009)
10.1021/ES034639P
In situ bioreduction of technetium and uranium in a nitrate-contaminated aquifer.
J. Istok (2004)
10.1038/nature03661
Extracellular electron transfer via microbial nanowires
G. Reguera (2005)
10.1016/0020-711x(92)90048-6
Cytochromes C: Evolutionary, Structural and Physicochemical Aspects
G. Moore (1990)
10.1016/J.MICRON.2003.10.029
Laccases: structure, reactions, distribution.
H. Claus (2004)
10.1007/s002480000018
Enrichment of Geobacter Species in Response to Stimulation of Fe(III) Reduction in Sandy Aquifer Sediments
O. Snoeyenbos-West (2000)
10.1111/J.1574-6968.1993.TB06066.X
Ferric reductase is associated with the membranes of anaerobically grown Shewanella putrefaciens MR-1
C. Myers (1993)
10.1016/S0960-9822(96)00737-3
Protein–protein interactions: PDZ domain networks
A. Fanning (1996)
10.1016/0003-2697(76)90067-1
An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels.
P. Thomas (1976)
10.1128/JB.187.17.5918-5926.2005
Adaptation to disruption of the electron transfer pathway for Fe(III) reduction in Geobacter sulfurreducens.
C. Leang (2005)
10.1128/AEM.54.6.1472-1480.1988
Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese.
D. Lovley (1988)
Characterization of a family of small periplasmic c-type cytochromes in Geobacter sulfurreducens and their role in Fe(III) respiration
L. N. DiDonato (2004)
10.1021/JP0718698
Mechanisms of electron transfer in two decaheme cytochromes from a metal-reducing bacterium.
Nicholas S. Wigginton (2007)
10.1186/1471-2180-7-16
Importance of c-Type cytochromes for U(VI) reduction by Geobacter sulfurreducens
E. Shelobolina (2006)
10.1074/jbc.271.47.29999
Stoichiometry of Subunits and Heme Content of Hemoglobin from the Earthworm Lumbricus terrestris*
H. Zhu (1996)
10.1016/J.BBAPAP.2006.04.017
The proteome of dissimilatory metal-reducing microorganism Geobacter sulfurreducens under various growth conditions.
Yan-Huai R. Ding (2006)
10.1042/BJ3590147
Isolation, characterization and gene sequence analysis of a membrane-associated 89 kDa Fe(III) reducing cytochrome c from Geobacter sulfurreducens.
T. Magnuson (2001)
10.1038/350413A0
Microbial reduction of uranium
D. Lovley (1991)
10.1042/BJ20020597
Biochemical and genetic characterization of PpcA, a periplasmic c-type cytochrome in Geobacter sulfurreducens.
J. Lloyd (2003)
10.1128/JB.186.12.4042-4045.2004
MacA, a diheme c-type cytochrome involved in Fe(III) reduction by Geobacter sulfurreducens.
J. Butler (2004)
10.1074/JBC.275.12.8515
Purification and Magneto-optical Spectroscopic Characterization of Cytoplasmic Membrane and Outer Membrane Multiheme c-Type Cytochromes from Shewanella frigidimarina NCIMB400*
S. J. Field (2000)
10.1111/J.1574-6968.2000.TB09063.X
Characterization of a membrane-bound NADH-dependent Fe(3+) reductase from the dissimilatory Fe(3+)-reducing bacterium Geobacter sulfurreducens.
T. Magnuson (2000)
10.1128/JB.108.1.422-430.1971
Localization and solubilization of colicin receptors.
S. Sabet (1971)
10.1007/BF01569889
Bioremediation of organic and metal contaminants with dissimilatory metal reduction
D. Lovley (2005)
10.1007/978-1-4615-4187-5_2
Bacterial Manganese and Iron Reduction in Aquatic Sediments
B. Thamdrup (2000)
10.1074/jbc.M203866200
Crystal Structures at Atomic Resolution Reveal the Novel Concept of “Electron-harvesting” as a Role for the Small Tetraheme Cytochrome c *
D. Leys (2002)
10.1371/journal.pone.0005628
Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells
K. Nevin (2009)
10.1016/0006-291X(69)90584-1
Proton magnetic resonance evidence for methionine-iron coordination in mammalian-type ferrocytochrome c.
C. C. Mcdonald (1969)
10.1002/prot.22260
Outer membrane cytochrome c, OmcF, from Geobacter sulfurreducens: High structural similarity to an algal cytochrome c6
P. R. Pokkuluri (2009)
10.1111/J.1574-6968.2007.00915.X
Evidence that OmcB and OmpB of Geobacter sulfurreducens are outer membrane surface proteins.
Xinlei Qian (2007)
10.1128/AEM.69.10.5884-5891.2003
Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer
R. T. Anderson (2003)
10.1128/JB.185.7.2096-2103.2003
OmcB, a c-type polyheme cytochrome, involved in Fe(III) reduction in Geobacter sulfurreducens.
C. Leang (2003)
10.1021/ES051493G
Biological enhancement of tetrachloroethene dissolution and associated microbial community changes.
B. Sleep (2006)
10.1128/AEM.62.5.1531-1536.1996
Isolation of Geobacter species from diverse sedimentary environments.
J. Coates (1996)
NMR of paramagnetic molecules in biological systems
I. Bertini (1986)
10.1016/S0167-4838(00)00135-7
Biphasic kinetic behavior of rat cytochrome P-4501A1-dependent monooxygenation in recombinant yeast microsomes.
K. Inouye (2000)
10.1128/AEM.57.3.867-870.1991
Electron Transport in the Dissimilatory Iron Reducer, GS-15.
Y. Gorby (1991)
10.1016/0306-3623(96)90070-1
The Physiology and Biochemistry of Prokaryotes
D. White (1995)
10.1016/0003-2697(85)90442-7
Measurement of protein using bicinchoninic acid.
P. Smith (1985)
10.1007/978-94-009-4007-9_1
The Geobiochemical Cycle of Iron
E. Murad (1988)
Proteomic investigation of c-type of cytochromes in Geobacter sulfurreducens
Y. R. Ding (2004)
10.1016/S0168-6445(03)00043-3
Microbial ferric iron reductases.
I. Schröder (2003)
10.1186/1471-2180-5-41
A novel Geobacteraceae-specific outer membrane protein J (OmpJ) is essential for electron transport to Fe (III) and Mn (IV) oxides in Geobacter sulfurreducens
E. Afkar (2005)
10.1128/AEM.64.9.3188-3194.1998
Localization and Solubilization of the Iron(III) Reductase of Geobacter sulfurreducens
S. Gaspard (1998)
10.1128/MICROBE.1.323.1
Microbial Energizers: Fuel Cells that Keep on Going
D. Lovley (2006)
10.1128/JB.174.11.3429-3438.1992
Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1.
C. Myers (1992)
10.1038/416767a
Geobacter metallireducens accesses insoluble Fe(iii) oxide by chemotaxis
S. Childers (2002)
10.1007/s00775-007-0278-y
Characterization of Shewanella oneidensis MtrC: a cell-surface decaheme cytochrome involved in respiratory electron transport to extracellular electron acceptors
R. S. Hartshorne (2007)
10.1128/AEM.65.7.3056-3063.1999
Microbial Communities Associated with Anaerobic Benzene Degradation in a Petroleum-Contaminated Aquifer
J. Rooney-Varga (1999)
10.1128/JB.187.13.4505-4513.2005
OmcF, a putative c-Type monoheme outer membrane cytochrome required for the expression of other outer membrane cytochromes in Geobacter sulfurreducens.
Byoung-Chan Kim (2005)
10.1128/AEM.72.4.2775-2782.2006
Geobacter lovleyi sp. nov. Strain SZ, a Novel Metal-Reducing and Tetrachloroethene-Dechlorinating Bacterium
Youlboong Sung (2006)
10.1128/AEM.01454-08
Kinetics of Reduction of Fe(III) Complexes by Outer Membrane Cytochromes MtrC and OmcA of Shewanella oneidensis MR-1
Z. Wang (2008)
10.1016/S0065-2911(04)49005-5
Dissimilatory Fe(III) and Mn(IV) reduction.
D. Lovley (2004)
10.1126/SCIENCE.1066771
Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments
D. Bond (2002)
10.1128/JB.188.8.3138-3142.2006
Two putative c-type multiheme cytochromes required for the expression of OmcB, an outer membrane protein essential for optimal Fe(III) reduction in Geobacter sulfurreducens.
Byoung-Chan Kim (2006)
10.1128/AEM.71.8.4728-4735.2005
Biological Control of Hog Waste Odor through Stimulated Microbial Fe(III) Reduction
J. Coates (2005)
10.1016/j.bbapap.2008.06.011
Proteome of Geobacter sulfurreducens grown with Fe(III) oxide or Fe(III) citrate as the electron acceptor.
Yan-Huai R. Ding (2008)
10.1038/NRMICRO1506
Bug juice: harvesting electricity with microorganisms
D. Lovley (2006)
10.2307/3225319
Methods in Cell Biology, Vol. 6
J. Corliss (1974)
Wireless? Additional Phenotypes of a Pilin-Deficient Mutant Weaken the Genetic Evidence for the Role of Microbial Nanowires in extracellular Electron Transfer
M. Izallalen (2008)
Chapter 7: Reduction of Iron and Humics in Subsurface Environments
D. Lovley (2001)
10.1128/AEM.00027-10
Purification and Characterization of OmcZ, an Outer-Surface, Octaheme c-Type Cytochrome Essential for Optimal Current Production by Geobacter sulfurreducens
K. Inoue (2010)
Chapter 1: Fe(III) and Mn(IV) Reduction
D. Lovley (2000)
10.1128/JB.180.14.3686-3691.1998
A periplasmic and extracellular c-type cytochrome of Geobacter sulfurreducens acts as a ferric iron reductase and as an electron carrier to other acceptors or to partner bacteria.
S. Seeliger (1998)
2008).Characterization of Pilin Function through Heterologous Complementation of the Geobacter sulfurreducens PilA-deficient Mutant
A. Klimes (2008)
Stimulation of dissimilatory Fe(III) reduction results in a predominance of Geobacter species in a variety of sandy aquifers
O. L. Snoeyenbos-West (2000)
10.1128/AEM.66.5.2248-2251.2000
Lack of Production of Electron-Shuttling Compounds or Solubilization of Fe(III) during Reduction of Insoluble Fe(III) Oxide by Geobacter metallireducens
K. Nevin (2000)
10.1126/SCIENCE.1088727
Genome of Geobacter sulfurreducens: Metal Reduction in Subsurface Environments
B. Methé (2003)
10.1016/0003-2697(84)90253-7
Specific indication of hemoproteins in polyacrylamide gels using a double-staining process.
R. T. Francis (1984)
10.1016/J.BBABIO.2007.01.012
Functional properties of type I and type II cytochromes c3 from Desulfovibrio africanus.
C. Paquete (2007)
10.1111/J.1462-2920.2006.01230.X
Detection of anaerobic toluene and hydrocarbon degraders in contaminated aquifers using benzylsuccinate synthase (bssA) genes as a functional marker.
Christian Winderl (2007)
10.1099/MIC.0.28864-0
A putative multicopper protein secreted by an atypical type II secretion system involved in the reduction of insoluble electron acceptors in Geobacter sulfurreducens.
T. Mehta (2006)
10.1128/JB.183.15.4468-4476.2001
Isolation and characterization of a soluble NADPH-dependent Fe(III) reductase from Geobacter sulfurreducens.
F. Kaufmann (2001)
10.1002/1439-7633(20011105)2:11<831::AID-CBIC831>3.0.CO;2-W
Cooperativity between Electrons and Protons in a Monomeric Cytochrome c3: The Importance of Mechano‐Chemical Coupling for Energy Transduction
R. Louro (2001)
10.1128/AEM.70.8.4911-4920.2004
Change in Bacterial Community Structure during In Situ Biostimulation of Subsurface Sediment Cocontaminated with Uranium and Nitrate
Nadia N North (2004)
10.1128/AEM.69.12.7467-7479.2003
Enumeration and Characterization of Iron(III)-Reducing Microbial Communities from Acidic Subsurface Sediments Contaminated with Uranium(VI)
Lainie Petrie (2003)
10.1016/J.JPOWSOUR.2007.12.123
The first demonstration of a microbial fuel cell as a viable power supply: Powering a meteorological buoy
L. Tender (2008)
10.1007/BF00290916
Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals
D. Lovley (2004)
10.1128/AEM.72.2.1558-1568.2006
Characterization of Metabolism in the Fe(III)-Reducing Organism Geobacter sulfurreducens by Constraint-Based Modeling
R. Mahadevan (2006)
10.1128/AEM.71.11.6870-6877.2005
Potential for Quantifying Expression of the Geobacteraceae Citrate Synthase Gene To Assess the Activity of Geobacteraceae in the Subsurface and on Current-Harvesting Electrodes
D. Holmes (2005)



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