Online citations, reference lists, and bibliographies.

Spectrophotometric Determination Of Protein Concentration

M. Simonian
Published 2004 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
This unit describes spectrophotometric and colorimetric methods for measuring the concentration of a sample protein in solution. Absorbance measured at 280 nm (A280) is used to calculate protein concentration by comparison with a standard curve or published absorptivity values for that protein (a280). Alternatively, absorbance measured at 205 nm (A205) is used to calculate the protein concentration. The A280 and A205 methods can be used to quantify total protein in crude lysates and purified or partially purified protein. A spectrofluorometer or a filter fluorometer can be used to measure the intrinsic fluorescence emission of a sample solution; this value is compared with the emissions from standard solutions to determine the sample concentration. The fluorescence emission method is used to quantify purified protein. This simple method is useful for dilute protein samples and can be completed in a short amount of time. There are two colorimetric methods: the Bradford colorimetric method, based upon binding of the dye Coomassie brilliant blue to the protein of interest, and the Lowry method, which measures colorimetric reaction of tyrosyl residues in the protein sample.
This paper references



This paper is referenced by
10.1002/cpcb.69
Analyzing Integrin‐Dependent Adhesion
A. Mould (2019)
10.4049/jimmunol.179.5.3144
Inhibition of HIV-1 Infectivity and Epithelial Cell Transfer by Human Monoclonal IgG and IgA Antibodies Carrying the b12 V Region1
N. Mantis (2007)
10.4172/2155-9821.1000306
The Effect of Hydrolysis and Protein Source on the Efficacy of Protein Hydrolysates as Plant Resistance Inducers against Powdery Mildew
Martina Cappelletti (2017)
Application of UV Light Scattering to Detect Reversible Self-association and Aggregation of Proteins in Solution
Japneet Kaur (2017)
Standard curve derived errors in colorimetric quantification of biotherapeutic proteins
Krishnanand Tiwari (2012)
The identification and characterization of novel haemolysin genes from Clostridium difficile
Zeina Subhi B. Alkudmani (2019)
Towards Novel Human Sweat Sensors
Bruno Carvalho dos Santos (2019)
10.4314/jfas.v9i5s.58
STATISTICAL DISCRIMINATION OF LATEX BETWEEN HEALTHY AND WHITE ROOT INFECTED HEVEA BRASILIENSIS TREE BASED ON PROTEIN CONCENTRATION
Mohd Suhaimi Sulaiman (2018)
10.1080/10934529.2018.1530537
Properties and potential application of efficient biosurfactant produced by Pseudomonas sp. KZ1 strain
A. Zdarta (2019)
10.1111/jfpp.14398
Ultrasound pretreatment of sunflower protein: Impact on enzymolysis, ACE-inhibition activity, and structure characterization
M. Dabbour (2020)
10.1111/1751-7915.12427
Strain improvement of Pichia kudriavzevii TY13 for raised phytase production and reduced phosphate repression
L. Qvirist (2017)
10.3390/toxins12080519
Production of Recombinant Gelonin Using an Automated Liquid Chromatography System
M. Berstad (2020)
10.7554/eLife.06659
SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis
Larissa Wilhelm (2015)
10.1128/EC.05211-11
The β-Arrestin-Like Protein Rim8 Is Hyperphosphorylated and Complexes with Rim21 and Rim101 To Promote Adaptation to Neutral-Alkaline pH
J. Gómez-Raja (2012)
10.1074/jbc.M117.779470
The ATP-dependent chromatin remodeling enzymes CHD6, CHD7, and CHD8 exhibit distinct nucleosome binding and remodeling activities
Benjamin J. Manning (2017)
10.6028/jres.122.033
Development of NIST Standard Reference Material 2082, a Pathlength Standard for Measurements in the Ultraviolet Spectrum
B. Lang (2017)
10.1074/jbc.M113.524421
A Histidine Aspartate Ionic Lock Gates the Iron Passage in Miniferritins from Mycobacterium smegmatis*
Sunanda Margrett Williams (2014)
10.1128/IAI.00324-06
Characterization of a Novel High-Affinity Monoclonal Immunoglobulin G Antibody against the Ricin B Subunit
Carolyn R McGuinness (2006)
10.1016/j.foodchem.2018.07.170
Interaction characterization of preheated soy protein isolate with cyanidin-3-O-glucoside and their effects on the stability of black soybean seed coat anthocyanins extracts.
Zhongqin Chen (2019)
10.3168/jds.2014-8920
Effect of homogenization and pasteurization on the structure and stability of whey protein in milk.
P. Qi (2015)
10.3168/jds.2015-10256
Physical and chemical changes in whey protein concentrate stored at elevated temperature and humidity.
M. Tunick (2016)
10.1111/JFBC.12982
Sonochemical action and reaction of edible insect protein: Influence on enzymolysis reaction-kinetics, free-Gibbs, structure, and antioxidant capacity.
Benjamin Kumah Mintah (2019)
10.1016/J.ACA.2019.02.024
Staring at protein-surfactant interactions: Fundamental approaches and comparative evaluation of their combinations - A review.
Y. Li (2019)
10.1002/btpr.1533
High‐throughput methods for miniaturization and automation of monoclonal antibody purification processes
K. Treier (2012)
10.1002/bmb.20918
Purification of a recombinant glutathione transferase from the causative agent of hydatidosis, Echinococcus granulosus
A. Fleitas (2016)
10.3389/fpls.2019.01432
Expression of a Chloroplast-Targeted Cyanobacterial Flavodoxin in Tomato Plants Increases Harvest Index by Altering Plant Size and Productivity
Martín L. Mayta (2019)
10.1186/s12934-014-0180-6
Quality assessment and optimization of purified protein samples: why and how?
B. Raynal (2014)
10.1002/cpcb.87
Western Blotting with Solutions containing Nanoliter Volumes of Antibody
David A Cruz Walma (2019)
10.1080/13506129.2018.1517736
Polymorph-specific distribution of binding sites determines thioflavin-T fluorescence intensity in α-synuclein fibrils
Arshdeep Sidhu (2018)
10.1101/601930
Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production
I. Tiukova (2019)
Thermodynamic characterization of hypertrophic cardiomyopathy associated troponin C mutations
K Rayani (2019)
10.1371/journal.pone.0120221
Modeling the Interaction between Quinolinate and the Receptor for Advanced Glycation End Products (RAGE): Relevance for Early Neuropathological Processes
I. Serratos (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar