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Overview Of Approaches To Preventing And Avoiding Proteolysis During Expression And Purification Of Proteins.

Barry J. Ryan, Gary T M Henehan
Published 2013 · Biology, Medicine
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Proteases are enzymes that cleave proteins. They occur widely in nature and play a fundamental role in cellular homeostasis; however, their presence can result in unwanted protein degradation during recombinant protein expression and purification. This unit introduces proteases, specifically focusing on the types commonly encountered during production of recombinant proteins. The strategies used to avoid and to prevent proteolysis are also described, with extensive consideration of the molecular, technical, and logistic methodologies involved.
This paper references
Compatible-Solute-Supported Periplasmic Expression of Functional Recombinant Proteins under Stress Conditions
Stefan Barth (2000)
Serine proteases and calpains fulfill important supporting roles in the apoptotic tragedy of the cellular opera
Peter Vandenabeele (2005)
The Structural Genomics Consortium
Molly Morgan Jones (2014)
Proteases in cellular regulation
P. Vandenabeele (2005)
MEROPS: the database of proteolytic enzymes, their substrates and inhibitors
Neil D. Rawlings (2012)
Strategies for achieving highlevel expression of genes in Escherichia coli
S C Makrides (1996)
Current Protocols in Protein Science Supplement
Molecular and Biotechnological Aspects of Microbial Proteases
M. Bhagwant Rao (1998)
Strategies for achieving high-level expression of genes in Escherichia coli.
Savvas C. Makrides (1996)
Development of a simple method for the recovery of recombinant proteins from the Escherichia coli periplasm
Carol A. French (1996)
Production of recombinant proteins by microbes and higher organisms.
Arnold L. Demain (2009)
Stabilization of proteins for storage.
Richard J. Simpson (2010)
Extracellular recombinant protein production from Escherichia coli
Ye Ni (2009)
Compatiblesolute-supported periplasmic expression of functional recombinant proteins under stress conditions
S Barth (2000)
Simple and Reliable Method To Precipitate Proteins from Bacterial Culture Supernatant
Randolph B. Caldwell (2004)
Protein Production and Purification
Isil Aksan Kurnaz (2015)
Development of a simple method for the recovery of recombinant proteins from the E.coli periplasm. Enzyme and Microbial Technoogy
Ek-M C French (1996)
RIPping and folding : Regulated intramembrane proteolysis
S. D. Buckingham (2003)
Tying up the loose ends: circular permutation decreases the proteolytic susceptibility of recombinant proteins.
Timothy A. Whitehead (2009)
Increasing the yield of soluble recombinant protein expressed in E. coli by induction during late log phase.
Chad Galloway (2003)
The product of the lon (capR) gene in Escherichia coli is the ATP-dependent protease, protease La.
Chin Ha Chung (1981)
Cold-inducible cloning vectors for low-temperature protein expression in Escherichia coli: application to the production of a toxic and proteolytically sensitive fusion protein.
Mirna Mujacic (1999)
Recent developments in difficult protein expression: a guide to E. coli strains, promoters, and relevant host mutations.
James C. Samuelson (2011)
On the discovery of precursor processing.
Donald Steiner (2011)
Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins
Chung-Hsien Cheng (2010)
Protein production and purification
Susanne Gräslund (2008)
Recombinant protein folding and misfolding in Escherichia coli
François Baneyx (2004)
MEROPS: the database of proteolytic enzymes, their substrates and inhibitors
Neil D. Rawlings (2014)
Lon and ClpP proteases participate in the physiological disintegration of bacterial inclusion bodies.
Andrea Vélez Vera (2005)
Chaperones and proteases--guardians of protein integrity in eukaryotic organelles.
Claudia Leidhold (2007)
Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost.
Wolfgang Peti (2007)
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
Hans Peter Sørensen (2005)
Recombinant protein secretion in Escherichia coli.
Filipe José Mergulhão (2005)
Avoiding proteolysis during protein chromatography.
Barry J. Ryan (2011)
How Proteolysis Drives the Cell Cycle
Randall W. King (1996)
Specific inhibitors prevent proteolytic degradation of recombinant proteins expressed in High Five cells.
Pia Møller Martensen (2001)
Correct folding of circularly permuted variants of a beta alpha barrel enzyme in vivo.
Kurt Luger (1989)
Proteases in cellular regulation
T C Vanaman (1999)
Proteases in Cellular Regulation Minireview Series*
Thomas C. Vanaman (1999)
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
Kay Terpe (2002)
Identification of C-terminal extensions that protect proteins from intracellular proteolysis.
James U. Bowie (1989)
RIPping and folding: Regulated intramembrane proteolysis. Signalling Scissors: New Perspectives on Proteases
S D Buckingham (2003)
Recent developments in production and biotechnological applications of cold-active microbial proteases
Mohammed Kuddus (2012)
Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements
Chung-Jr Huang (2011)

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