Mitotic Chromosome Preparations From Mouse Cells For Karyotyping
E. Akeson, M. Davisson
Published 2001 · Biology, Medicine
Download PDFAnalyze on Scholarcy
This unit contains protocols for the preparation of mitotic chromosomes from mouse peripheral blood, Giemsa banding of those chromosomes, and classification into a karyotype, including recognition of some common pitfalls of misidentification and information for determining aberrant chromosomes. The methods described can be used to identify visible chromosomal rearrangements and their precise cytological breakpoints in the living mouse. In conjunction with fluorescent in situ hybridization (FISH), the metaphase spreads can also be used for the linear placement of loci on a chromosome and for determining the insertion site(s) of a foreign transgene.
This paper references
High resolution G-banded chromosomes of the mouse
J. Sawyer (1987)
Standardized nomenclature for inbred strains of mice: seventh listing for the International Committee on Standardized Genetic Nomenclature for Mice.
J. Staats (1980)
A method for preparing chromosomes from peripheral blood of the mouse.
P. R. Barren (1982)
The mechanism of C- and G-banding of chromosomes.
D. Comings (1973)
Heritability of the phytohemagglutinin responsiveness of lymphocytes and its relationship to leukemogenesis.
H. Heiniger (1975)
Cytogenetic methods for the mouse: preparation of chromosomes, karyotyping, and in situ hybridization.
J. Lee (1990)
Mouse chromosomes identified by trypsin-Giemsa (T-G) banding.
D. Wurster (1972)
tromeric heterochromatin variants
E. C. Akeson (2000)
Karyotyping and sexing of gametes, embryos and fetuses and in situ hybridization to chromosomes
E. P. Evans (1987)
A photographic representation of the G-banded structure of mouse chromosomes at different stages of condensation with a summary of the main features (landmarks) of each chromosome for identification
Simplified whole blood method for evaluating in vitro lymphocyte reactivity of laboratory animals.
T. Han (1972)
Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.
P. Nowell (1960)
Analysing mouse chromosomal rearrangements with G-banded chromosomes.
E. Akeson (2000)
Centromeric heterochromatin variants.
M. Davisson (1996)
An improved method for preparing G-banded chromosomes from mouse peripheral blood.
M. Davisson (1987)
S. Rastan (1971)
Identifying mouse chromosomes with the ASG technique.
R. Buckland (1971)
Mouse care and husbandry
C. M. Abbott (1994)
Standard karyotype of the mouse, Mus musculus.
Identification of the mouse chromosomes by quinacrine mustard staining.
U. Francke (1971)
Functional heterogeneity of murine lymphoid cells. I. Responsiveness to and surface binding of concanavalin A and phytohemagglutinin.
J. Stobo (1972)
This paper is referenced by
Cell cycle deregulation by methyl isocyanate: Implications in liver carcinogenesis.
Hariom Panwar (2014)
Occurrence of perfluorinated alkylated substances (PFASs) and evaluation of potential genotoxic effects in small rodents from a skiing area, Granåsen, Norway
Berta Pérez Vázquez (2018)
Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn
L. Reinholdt (2011)
Large interrelated clusters of repetitive elements (REs) and RE arrays predominantly represent reference mouse chromosome Y
K. Lee (2012)
Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation
D. Grégoire (2008)
Polymerase chain reaction method to identify Down syndrome model segmentally trisomic mice.
N. Ramakrishna (2005)
Complex oncogenic translocations with gene amplification are initiated by specific DNA breaks in lymphocytes.
S. M. Wright (2009)
Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
Elsa Abranches (2013)
! Two New Mouse Model Centers at The Jackson Laboratory ! Selected Publications Authored By Jackson Laboratory Scientists
Jackson Labora (2001)
Diverse Hematological Malignancies Including Hodgkin-Like Lymphomas Develop in Chimeric MHC Class II Transgenic Mice
S Raffegerst (2009)
Toxic effects of maternal exposure to silver nanoparticles on mice fetal development during pregnancy.
Maryam Mozafari (2019)