Online citations, reference lists, and bibliographies.
← Back to Search

Uncompensated Polychromatic Analysis Of Mitochondrial Membrane Potential Using JC‐1 And Multilaser Excitation

S. De Biasi, L. Gibellini, A. Cossarizza
Published 2015 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
The lipophilic cation JC‐1 (5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethyl‐benzimidazolyl carbocyanine iodide) has been used for more than 20 years as a specific dye for measuring mitochondrial membrane potential (ΔΨm). In this unit, we revise our original protocol (that made use of a single 488 nm laser for the detection of monomers and aggregates, and where compensation was an important step) to use dual‐laser excitation. Moreover, thanks to recently developed multilaser instruments and novel probes for surface and intracellular markers, JC‐1 can be utilized by polychromatic flow cytometry to simultaneously detect, without any compensation between fluorescences, ΔΨm along with other biological parameters, such as apoptosis and the production of reactive oxygen species. © 2015 by John Wiley & Sons, Inc.
This paper references
Spectral Absorption and Fluorescence of Dyes in the Molecular State
EDWIN E. Jelley (1936)
J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential.
M. Reers (1991)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1).
A. Cossarizza (1993)
Mitochondrial modifications during rat thymocyte apoptosis: a study at the single cell level.
A. Cossarizza (1994)
Protective effect of N-acetylcysteine in tumor necrosis factor-alpha-induced apoptosis in U937 cells: the role of mitochondria.
A. Cossarizza (1995)
Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo
N. Zamzami (1995)
Mitochondria are selective targets for the protective effects of heat shock against oxidative injury.
B. Polla, (1996)
Mitochondria alterations and dramatic tendency to undergo apoptosis in peripheral blood lymphocytes during acute HIV syndrome
Andrea Cossarizza (1997)
The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis
R. Kluck (1997)
JC‐1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess ΔΨ changes in intact cells: implications for studies on mitochondrial functionality during apoptosis
S. Salvioli (1997)
Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD‐specific caspase activation and independently of mitochondrial transmembrane depolarization
E. Bossy-Wetzel (1998)
Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: analysis at the single cell and single organelle level.
S. Salvioli (2000)
Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC‐1
A. Cossarizza (2001)
Mitochondrial functionality and mitochondrial DNA content in lymphocytes of vertically infected human immunodeficiency virus-positive children with highly active antiretroviral therapy-related lipodystrophy.
A. Cossarizza (2002)
Polychromatic flow cytometry: A rapid method for the reduction and analysis of complex multiparameter data
U. Petrausch (2006)
Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry
L. Troiano (2007)
Subject classification obtained by cluster analysis and principal component analysis applied to flow cytometric data
E. Lugli (2007)
Mitochondrial membrane permeabilization in cell death.
G. Kroemer (2007)
Data analysis in flow cytometry: The future just started
E. Lugli (2010)
SPICE: Exploration and analysis of post‐cytometric complex multivariate datasets
M. Roederer (2011)
Mitochondria and the Autophagy–Inflammation–Cell Death Axis in Organismal Aging
D. Green (2011)
JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry
A. Perelman (2012)
Mitochondria: master regulators of danger signalling
L. Galluzzi (2012)
The protease inhibitor atazanavir triggers autophagy and mitophagy in human preadipocytes
L. Gibellini (2012)
Annexin-Phospholipid Interactions. Functional Implications
M. Lizarbe (2013)
Flavokawain A Induces Apoptosis in MCF-7 and MDA-MB231 and Inhibits the Metastatic Process In Vitro
N. Abu (2014)
VDAC phosphorylation, a lipid sensor influencing the cell fate.
C. Martel (2014)
Honey Bee Hemocyte Profiling by Flow Cytometry
William J. Marringa (2014)
Source for The Handbook-A Guide to Fluorescent Probes and Labeling Technologies from Invitrogen/Molecular Probes

This paper is referenced by
The Potential Use of Sialic Acid From Edible Bird’s Nest to Attenuate Mitochondrial Dysfunction by In Vitro Study
A. Rashed (2021)
Capsaicin protects cardiomyocytes against lipopolysaccharide-induced damage via 14-3-3γ-mediated autophagy augmentation
Y. Qiao (2021)
Zirconia Nanoparticles Induce HeLa Cell Death Through Mitochondrial Apoptosis and Autophagy Pathways Mediated by ROS
Yinghui Shang (2021)
Naringin‐generated ROS promotes mitochondria‐mediated apoptosis in Candida albicans
Heesu Kim (2021)
Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
C. Cooksey (2021)
GW9508 ameliorates cognitive impairment via the cAMP-CREB and JNK pathways in APPswe/PS1dE9 mouse model of Alzheimer's disease
Yuhang Gong (2020)
Curcumol inhibits KLF5-dependent angiogenesis by blocking the ROS/ERK signaling in liver sinusoidal endothelial cells.
Liyuan Gao (2020)
A small natural molecule CADPE kills residual colorectal cancer cells by inhibiting key transcription factors and translation initiation factors
G. Zheng (2020)
Capsaicin Alleviates the Deteriorative Mitochondrial Function by Upregulating 14-3-3η in Anoxic or Anoxic/Reoxygenated Cardiomyocytes
Y. Qiao (2020)
The Effects of Different Fluorescent Indicators in Observing the Changes of the Mitochondrial Membrane Potential during Oxidative Stress-Induced Mitochondrial Injury of Cardiac H9c2 Cells.
Y. Sun (2020)
ConBr lectin modulates MAPKs and Akt pathways and triggers autophagic glioma cell death by a mechanism dependent upon caspase-8 activation.
Ingrid A V Wolin (2020)
Toxoplasma gondii ROP18 inhibits human glioblastoma cell apoptosis through a mitochondrial pathway by targeting host cell P2X1
L. Zhou (2019)
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
A. Cossarizza (2019)
Cytotoxic potentials of S. cumini methanolic seed kernel extract in human hepatoma HepG2 cells
Ashwin Kumar Solai Prakash (2019)
Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells
W. F. Marei (2019)
Mitochondria-targeted therapy rescues development and quality of embryos derived from oocytes matured under oxidative stress conditions: a bovine in vitro model.
W. F. Marei (2019)
Regulating intracellular ROS signal by a dual pH/reducing-responsive nanogels system promotes tumor cell apoptosis
Kai Dong (2019)
Graphene oxide induces p62/SQSTM-dependent apoptosis through the impairment of autophagic flux and lysosomal dysfunction in PC12 cells.
X. Feng (2018)
Toxoplasma gondii ROP18 Inhibits Human Glioblastoma Cell Apoptosis through Mitochondrial Pathway by Targeting Host Cell P2X1
Lijuan Zhou (2018)
Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress
Gonzalo Ferreira (2018)
Guidelines for the use of flow cytometry and cell sorting in immunological studies
A. Cossarizza (2017)
Sustained early disruption of mitochondrial function contributes to arsenic-induced prostate tumorigenesis
B. Singh (2016)
Quantification of mitochondrial reactive oxygen species in living cells by using multi‐laser polychromatic flow cytometry
S. de Biasi (2016)
Inhibition of Lon protease by triterpenoids alters mitochondria and is associated to cell death in human cancer cells
L. Gibellini (2015)
Semantic Scholar Logo Some data provided by SemanticScholar