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Immobilization Of Cholesterol Oxidase And Potassium Ferricyanide On Dodecylbenzene Sulfonate Ion‐doped Polypyrrole Film

Arun Kumar, Rajesh, A. Chaubey, S. Grover, B. D. Malhotra
Published 2001 · Chemistry

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Dodecylbenzene sulfonate (DBS)-doped polypyrrole (PPY) conducting polymer films were electrochemically deposited onto the indium-tin-oxide (ITO)-coated glass plates in aqueous medium. The enzyme cholesterol oxidase (ChOx) was immobilized on these DBS–PPY films by a physical adsorption technique. These ChOx-immobilized DBS–PPY films were characterized by ultraviolet–visible and Fourier transform infrared spectroscopy. The enzyme activity studies indicate that ∼40% of ChOx leaches out from the ChOx/DBS–PPY film. The ChOx activity in the ChOx/DBS–PPY film was assayed as a function of cholesterol concentration. The results of amperometric measurements conducted on ChOx/DBS–PPY/ITO film show linearity over the range 2–8 mM of cholesterol solution. The ChOx/DBS–PPY/ITO electrodes exhibit a response time of 30 s and are stable for ∼3 months at 4 °C. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 82: 3486–3491, 2001
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