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Deletion Studies To Reveal The Basis For Size Discrepancy In Proliferating Cell Nuclear Antigen

C. P. Liang, Y. C. Lee, Y. Liu
Published 1992 · Biology, Medicine

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Proliferating cell nuclear antigen (PCNA), an essential component for DNA replication in eukaryotes, is a highly conserved nonhistone nuclear protein of 261 amino acids. The molecular weight of mammalian PCNA, estimated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), differs notably from that predicted by the cDNA sequences, that is, 36000 in comparison with 29261 and 28748 for human and rat PCNA, respectively. To investigate if this discrepancy is due to posttranslational modifications, we studied the PCNA protein synthesized by an in vitro transcription/translation system as well as the protein overproduced in bacteria. We found that both PCNA protein samples were indistinguishable from the authentic protein from the protein mobility in SDS‐PAGE. The finding indicates that the size discrepancy is not due to the posttranslational modifications. Hence, the size discrepancy may be due to the protein sequence per se, namely a sequence‐related anomaly in SDS‐PAGE. Results from the analyses of a series of PCNA derivatives with various lengths of C‐ or N‐terminal deletion indicate that the putative sequence is in the region of residues 128–150.
This paper references
Multiple replication factors augment DNA synthesis by the two eukaryotic DNA polymerases, alpha and delta.
T. Tsurimoto (1989)
Epidermal growth factor inhibits the synthesis of the nuclear protein cyclin in A431 human carcinoma cells.
R. Bravo (1984)
Inhibition of cellular proliferation by antisense oligodeoxynucleotides to PCNA cyclin.
D. Jaskulski (1988)
Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins.
J. Almendral (1987)
Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes.
W. D. de Jong (1978)
Differential binding of sodium dodecyl sulfate to amino acids as evidenced by elution from Sephadex.
F. Maley (1977)
Requirement for proliferating cell nuclear antigen expression during stages of the Chinese hamster ovary cell cycle.
Y. Liu (1989)
A nuclear antigen associated with cell proliferation and blast transformation
Y. Takasaki (1981)
A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells
R. Bravo (1980)
Cyclin: a nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cells.
J. Celis (1984)
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K. Weber (1969)
Murine DNA polymerase beta gene: mapping of transcription initiation sites and the nucleotide sequence of the putative promoter region.
M. Yamaguchi (1987)
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
M. Kozak (1987)
Molecular cloning and structural analysis of mouse gene and pseudogenes for proliferating cell nuclear antigen.
M. Yamaguchi (1991)
Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene.
G. A. Bauer (1990)
Nucleotide sequences from the adenovirus-2 genome.
T. Gingeras (1982)
Immunochemical and biochemical analysis of the proliferating cell nuclear antigen (PCNA) in HeLa cells.
M. R. Sadaie (1986)
Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
A. H. Rosenberg (1987)
Identity of the proliferating cell nuclear antigen and cyclin
M. Mathews (1984)
Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ
R. Bravo (1987)
Insights into native epitopes of proliferating cell nuclear antigen using recombinant DNA protein products
J. Huff (1990)
Synthesis of the nuclear protein cyclin does not correlate directly with transformation in quail embryo fibroblasts.
R. Bravo (1985)
Structure of the human gene for the proliferating cell nuclear antigen.
S. Travali (1989)
SDS—PAGE strongly overestimates the molecular masses of the neurofilament proteins
E. Kaufmann (1984)
Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation.
H. Persson (1984)
Gene expression of PCNA/cyclin in adult tissues and the R3230AC mammary tumor of rat.
Y. Liu (1989)
Identification of a nuclear and of a cytoplasmic polypeptide whose relative proportions are sensitive to changes in the rate of cell proliferation.
R. Bravo (1981)
Upstream sequences of rice proliferating cell nuclear antigen (PCNA) gene mediate expression of PCNA-GUS chimeric gene in meristems of transgenic tobacco plants.
S. Kosugi (1991)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
Autoantibody to a nuclear antigen in proliferating cells.
K. Miyachi (1978)
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein
G. Prelich (1987)
Molecular cloning of cDNA coding for rat proliferating cell nuclear antigen (PCNA)/cyclin.
K. Matsumoto (1987)
Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replication.
R. Bravo (1986)
Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replication.
K. Ogata (1987)

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