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CDNA Labeling Strategies For Microarrays Using Fluorescent Dyes
J. H. Do, D. Choi
Published 2007 · Biology
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Microarray analysis has become a key experimental tool in the study of genome‐wide patterns of gene expression. The labeling step of target molecules such as cDNA or cRNA plays a key role in a microarray experiment because the amount of mRNA is measured indirectly by the labeled molecules. In this paper, the most widely used cDNA labeling strategies in microarray experiments are reviewed in detail, including direct labeling and indirect labeling methods along with a discussion of the merits and disadvantages of these methods. Furthermore, various RNA amplification approaches were surveyed to obtain a target nucleic acid sufficient for microarray experiments from minute amounts of mRNA. Finally, the labeling strategies of commonly used microarray platforms (e.g., Affymetrix GeneChip®, CodeLink™ Bioarray, Agilent and spotted microarrays) were compared.
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