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Characterization Of Poly(ethylene Glycol) Gels With Added Collagen For Neural Tissue Engineering.
Rebecca A Scott, Laura M. Marquardt, R. Willits
Published 2010 · Materials Science, Medicine
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Over the past decade, it has been increasingly recognized that both chemical and mechanical properties of scaffolds influence neural cell behavior, ranging from growth to differentiation to migration. However, mechanical properties are difficult to control for in the design of scaffolds for nerve regeneration, as properties change over time for most biologically derived scaffolds. The focus of this project was to examine how the mechanical properties of a nondegradable scaffold, poly(ethylene glycol) (PEG) gels, influenced nerve cell behavior. Low concentration PEG gels, of 3, 4, or 5% PEG, with added collagen to alter chemical properties were examined for both their mechanical properties and their ability to support nerve expression and extension. Stiffness (G*) significantly increased with increased PEG concentration. The addition of chemically conjugated collagen significantly decreased the stiffness compared to plain gels. This phenomenon was confirmed to be an effect of the conjugate, and not the protein itself, as G* of gels containing conjugate, but no protein, was not significantly different than G* of gels with conjugated protein. PC12 cell neurite expression increased with decreasing PEG and increasing collagen concentration. At its best, the expression approached the value on collagen-coated tissue culture plastic, which is a substantial improvement over previous studies on PEG. Neurite extension of dorsal root ganglia was also improved on these same gels over gels with either higher PEG concentration or lower collagen amount. Overall, these results suggest that exploration of lower stiffness materials is necessary to improve neurite growth and extension in three-dimensional synthetic scaffolds.
This paper references
Hydrogels for tissue engineering: scaffold design variables and applications.
Jeanie L Drury (2003)
Synthesis and characterization of polymer-(multi)-peptide conjugates for control of specific cell aggregation.
N. Belcheva (1998)
Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain
S. Bryant (2004)
Cell-binding Peptides Conjugated to Poly(ethylene glycol) Promote Neural Cell Aggregation
Weiguo Dai (1994)
Effects of Extracellular Matrix Components on Axonal Outgrowth from Peripheral Nerves of Adult Animalsin Vitro
D. Tonge (1997)
Adhesive and mechanical properties of hydrogels influence neurite extension.
Jonathan Gunn (2005)
A cytomechanical investigation of neurite growth on different culture surfaces
P. Lamoureux (1992)
Evaluation of a new reagent for covalent attachment of polyethylene glycol to proteins.“
S. Zalipsky (1992)
Release of protein from highly cross-linked hydrogels of poly(ethylene glycol) diacrylate fabricated by UV polymerization.
M. Mellott (2001)
Compositional alterations of fibrin-based materials for regulating in vitro neural outgrowth.
O. Sarig-Nadir (2008)
A laminin and nerve growth factor-laden three-dimensional scaffold for enhanced neurite extension.
X. Yu (1999)
Poly(ethylene glycol)-containing hydrogels in drug delivery.
N. Peppas (1999)
Differences between the effect of anisotropic and isotropic laminin and nerve growth factor presenting scaffolds on nerve regeneration across long peripheral nerve gaps.
Mahesh Chandra Dodla (2008)
Cell-binding domain context affects cell behavior on engineered proteins.
S. Heilshorn (2005)
Substrates for cell adhesion prepared via active site-directed immobilization of a protein domain.
W. Murphy (2004)
Laminin oligopeptide derivatized agarose gels allow three‐dimensional neurite extension in vitro
R. Bellamkonda (1995)
Tethered protein/peptide-surface-modified hydrogels
Jingjing Bi (2004)
Effect of collagen gel stiffness on neurite extension
R. Willits (2004)
Hydrogel-based three-dimensional matrix for neural cells.
R. Bellamkonda (1995)
Regulation of Neurite Outgrowth by Integrin Activation
J. K. Ivins (2000)
Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering.
B. Mann (2001)
Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels.
S. Bryant (2002)
Polyethylene oxide as a biomaterial
E. Merrill (1983)
Tissue adhesiveness and host response of in situ photopolymerizable interpenetrating networks containing methylprednisolone acetate.
J. L. Zilinski (2004)
Mechanical properties of layered poly (ethylene glycol) gels.
S. L. Skornia (2007)
Investigation of Molecular Transport and Distributions in Poly(ethylene glycol) Hydrogels with Confocal Laser Scanning Microscopy
Andrew W. Watkins (2005)
A collagen‐based nerve guide conduit for peripheral nerve repair: An electrophysiological study of nerve regeneration in rodents and nonhuman primates
S. Archibald (1991)
Three-dimensional extracellular matrix engineering in the nervous system.
M. Borkenhagen (1998)
Applied electric field enhances DRG neurite growth: influence of stimulation media, surface coating and growth supplements.
M. Wood (2009)
In vivo Stability of Poly(ethylene glycol)-Collagen Composites
W. Rhee (1997)
Conditional β1-integrin gene deletion in neural crest cells causes severe developmental alterations of the peripheral nervous system
T. Pietri (2004)
Design and characterization of poly(ethylene glycol) photopolymerizable semi-interpenetrating networks for chondrogenesis of human mesenchymal stem cells.
Amanda N Buxton (2007)
Maintaining bioactivity of NGF for controlled release from PLGA using PEG.
P. Johnson (2008)
Type I collagen in solution. Structure and properties of fibril fragments.
F. Silver (1980)
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The impact of laminin on 3D neurite extension in collagen gels.
Katelyn E Swindle-Reilly (2012)
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Modular poly(ethylene glycol) scaffolds provide the ability to decouple the effects of stiffness and protein concentration on PC12 cells.
Rebecca A Scott (2011)
Development and in vitro characterization of three dimensional biodegradable scaffolds for peripheral nerve tissue engineering
N. Zhu (2012)
Laminin-Functionalized Polyethylene Glycol Hydrogels for Nucleus Pulposus Regeneration
A. T. Francisco (2013)
Photocrosslinkable laminin-functionalized polyethylene glycol hydrogel for intervertebral disc regeneration.
A. Francisco (2014)
Increasing capillary diameter and the incorporation of gelatin enhance axon outgrowth in alginate-based anisotropic hydrogels.
K. Pawar (2011)
Creation of a Mechanical Gradient Peg-Collagen Scaffold by Photomasking Techniques
P. Patterson (2013)
Angioneural Crosstalk in Scaffolds with Oriented Microchannels for Regenerative Spinal Cord Injury Repair
Aybike Saglam (2012)
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Hannah L. Cebull (2017)
Development and Analysis of Semi-Interpenetrating Polymer Networks for Brain Injection in Neurodegenerative Disorders
M. Tunesi (2013)
A three-dimensional multiporous fibrous scaffold fabricated with regenerated spider silk protein/poly(l-lactic acid) for tissue engineering.
Qiaozhen Yu (2015)
Functionalizing artificial nerve guides to promote regeneration and recovery after peripheral nerve injuries
G. Pérez (2016)
Nanotopography induced contact guidance of the F11 cell line during neuronal differentiation: a neuronal model cell line for tissue scaffold development.
P. Wieringa (2012)
Hydrogel-assisted neuroregeneration approaches towards brain injury therapy: A state-of-the-art review
V. A. Kornev (2018)
Growth Factor Gradient Formation and Release from PEG Microspheres for Nerve Regeneration.
Jacob L Roam (2015)
Naturally and synthetic smart composite biomaterials for tissue regeneration.
R. Pérez (2013)
Aqueous biphasic microprinting approach to tissue engineering.
H. Tavana (2011)
Protein-hydrogel interactions in tissue engineering: mechanisms and applications.
S. Zustiak (2013)
Characteristics of precipitation-formed polyethylene glycol microgels are controlled by molecular weight of reactants.
S. Thompson (2013)
Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds.
C. Chen (2012)
Robust and Degradable Hydrogels from Poly(ethylene glycol) and Semi-Interpenetrating Collagen
Charles W. Peak (2014)
Enzyme-mediated preparation of hydrogels composed of poly(ethylene glycol) and gelatin as cell culture platforms
K. Moriyama (2015)
Tuning the Mechanical Properties of Poly(Ethylene Glycol) Microgel-Based Scaffolds to Increase 3D Schwann Cell Proliferation.
Wenda Zhou (2016)
Synthetic biomaterials for engineering neural tissue from stem cells
S. Willerth (2018)
Human Adventitial Fibroblast Phenotype Depends on the Progression of Changes in Substrate Stiffness.
Rebecca A Scott (2020)
Vascular endothelial growth factor-loaded injectable hydrogel enhances plasticity in the injured spinal cord.
A. des Rieux (2014)
Preliminary Study on the Fabrication of Alginate/Hyaluronic Acid Scaffolds for Spinal Cord Injury Repair
Mindan Wang (2012)
Engineering therapies in the CNS: What works and what can be translated
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