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Integral Membrane Glycoproteins Related To Cell–Substratum Adhesion In Mammalian Cells

C. Damsky, K. Knudsen, C. Buck
Published 1982 · Biology, Medicine

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Broad spectrum antisera have been raised against surface membrane‐derived material from baby hamster kidney cells and mouse mammary tumor epithelial cells. These antisera disrupt cell–substratum adhesion in their respective cell types. Using an antibody neutralization (blocking) assay, adhesion‐related glycoproteins have been isolated from non‐ionic detergent extracts of each cell type. The purified material in each case consisted of a restricted population of glycoproteins of approximately 120,000–160,000 Mr. Purified material from each system blocked the disruption of adhesion induced by the heterologous antiserum on either cell type. The antisera were capable of disrupting cell–substratum adhesion of a large number of cell types and species sources. In addition, antibody blocking activity could be detected from partially purified extracts of several adult hamster cell types and a variety‐of cultured cell types. Thus, in addition to having similar substratum–associated glycoproteins (eg, fibronectin) and cytoskeleton‐associated proteins (eg, α‐actinin and vinculin) cells from different species and tissue sources appear to have a relatively conserved class of integral membrane glycoproteins involved in cell substratum–adhesion.
This paper references
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
10.1038/275179A0
Fibronectins—adhesive glycoproteins of cell surface and blood
K. Yamada (1978)
Topological arrangement of the major structural features of fibronectin.
D. Wagner (1980)
10.1016/0092-8674(80)90622-4
Cell-Cell adhesion molecule: Identification of a glycoprotein relevant to the Ca2+-independent aggregation of chinese hamster fibroblasts
H. Urushihara (1980)
Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina.
J. Thiery (1977)
10.1083/JCB.88.3.473
Role of collagenous matrices in the adhesion and growth of cells
H. Kleinman (1981)
10.1016/S0092-8674(80)80035-3
Microinjection and localization of a 130K protein in living fibroblasts: a relationship to actin and fibronectin
K. Burridge (1980)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
10.1083/JCB.80.2.403
Studies on the function of cell surface glycoproteins. II. Possible role of surface glycoproteins in the control of cytoskeletal organization and surface morphology
C. Damsky (1979)
Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments.
K. Yamada (1980)
10.1083/JCB.89.2.173
Manipulation of cell-cell and cell-substratum interactions in mouse mammary tumor epithelial cells using broad spectrum antisera
C. Damsky (1981)
10.1083/JCB.80.2.385
Studies on the function of cell surface glycoproteins. I. Use of antisera to surface membranes in the identification of membrane components relevant to cell-substrate adhesion
D. Wylie (1979)
Adhesion among neural cells of the chick embryo. I. An immunological assay for molecules involved in cell-cell binding.
R. Brackenbury (1977)
Association between ankyrin and the cytoplasmic domain of band 3 isolated from the human erythrocyte membrane.
V. Bennett (1980)
10.1016/0092-8674(78)90272-6
Relationships between fibronectin (LETS protein) and actin
R. Hynes (1978)
10.1016/S0074-7696(08)62241-X
Cellular adhesiveness and extracellular substrata.
F. Grinnell (1978)
10.1016/0092-8674(79)90109-0
Cross-linking of fibronectin to sulfated proteoglycans at the cell surface
M. Perkins (1979)



This paper is referenced by
10.1007/978-1-4684-1197-3
Axonal Transport in Neuronal Growth and Regeneration
J. Elam (1984)
10.2478/hepo-2018-0016
Expression of genes modulated by epigallocatechin-3-gallate in breast cancer cells
Anna Bogacz (2018)
Structure / Function Analysis of the Integrin / 31 Subunit by Epitope Mapping
Daw-Tsun Shih (2002)
10.1042/CS091018P
A Role in Migration for the αVβ1 Integrin Expressed on Oligodendrocyte Precursors
R. Milner (1996)
10.1007/978-1-4613-2299-3_3
Integral Membrane Adhesion Glycoproteins: What is their Fate During Metastasis?
C. Damsky (1986)
The role of E-cadherin and integrins in mesoderm differentiation and migration at the mammalian primitive streak.
C. Burdsal (1993)
10.1016/0012-1606(85)90400-2
Dual adhesion systems of chick myoblasts.
D. Gibralter (1985)
10.1016/B978-0-12-508470-3.50010-9
Fibronectin—Cytoskeleton Relationships
I. Singer (1989)
10.1083/JCB.106.4.1331
Expression and function of cell surface extracellular matrix receptors in mouse blastocyst attachment and outgrowth
A. Sutherland (1988)
10.1002/IJC.2910330609
Unequal forms of 140–110 kD glycoproteins in B16 melanoma cells with differing detachment properties and metastatic behavior: Influence of bromodeoxyuridine
M. Rieber (1984)
10.1258/RSMSMJ.53.1.60D
The Effect of Carnitine on Treatment of Familial Chylomicronemia Syndrome Presenting with Acute Pancreatitis
N. Urgancı (2008)
10.1007/978-1-4684-1197-3_6
Axonal Transport of Glycoproteins in Regenerating Nerve
J. Elam (1984)
10.1016/0309-1651(85)90056-6
Surface glycoproteins of cultured human umbilical vein endothelial cells.
M. Hormia (1985)
10.1161/01.RES.67.1.175
Integrin receptors on aortic smooth muscle cells mediate adhesion to fibronectin, laminin, and collagen.
R. Clyman (1990)
10.1385/1-59259-249-X:135
Preparing a polyclonal antibody to mouse beta 1 integrin with function-blocking activity.
G. Edwards (1999)
10.1007/978-1-4899-2013-3_18
CSAT Antibody Interferes with in Vivo Migration of Somitic Myoblast Precursors into the Body Wall
T. Jaffredo (1986)
10.1083/JCB.105.5.2347
Interactions of a neuronal cell line (PC12) with laminin, collagen IV, and fibronectin: identification of integrin-related glycoproteins involved in attachment and process outgrowth
K. Tomaselli (1987)
10.1002/IJC.2910340322
Relationship of a novel extracellular matrix glycoprotein to cell detachment in highly metastatic B16 melanoma: Modulating effect of bromodeoxyuridine
M. Rieber (1984)
Melanoma cell adhesion to basement membrane mediated by integrin-related complexes.
R. Kramer (1989)
Myoblast migration specifically inhibited in the chick embryo by grafted CSAT hybridoma cells secreting an anti-integrin antibody.
T. Jaffredo (1988)
10.1126/science.7531366
Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix
N. Boudreau (1995)
10.1002/JCB.1982.240180204
Expression of Adhesion‐Related Membrane Components in Adherent Versus Nonadherent Hamster Melanoma Cells
K. Knudsen (1982)
An tV / ^-like Integrin Receptor on Rat Aortic Smooth Muscle Cells Mediates Adhesion to Laminin and Collagen Types I and IV
R. Clyman (2005)
10.1016/0092-8674(83)90379-3
Identification and purification of a cell surface glycoprotein mediating intercellular adhesion in embryonic and adult tissue
C. Damsky (1983)
10.1007/978-1-4684-7464-0_1
Integral Membrane Glycoproteins in Cell-Cell and Cell-Substratum Adhesion
C. Damsky (1984)
10.1016/0014-4827(84)90632-3
Interaction of fibronectin-coated beads with CHO cells.
M. Schwarz (1984)
10.1002/JCP.1041390209
Human microvascular endothelial cells express integrin‐related complexes that mediate adhesion to the extracellular matrix
Y. F. Cheng (1989)
10.1111/J.1432-0436.1992.TB00670.X
Disruption of the cytoskeleton-extracellular matrix linkage promotes the accumulation of plasminogen activators in F9 derived parietal endoderm.
R. Snyder (1992)
10.1083/JCB.115.5.1383
Control of mammary epithelial differentiation: basement membrane induces tissue-specific gene expression in the absence of cell-cell interaction and morphological polarity
C. Streuli (1991)
10.1126/SCIENCE.4012302
Selective inhibition of fibronectin-mediated cell adhesion by monoclonal antibodies to a cell-surface glycoprotein.
P. Brown (1985)
10.1016/0896-6273(88)90170-5
Identification of a neuronal laminin receptor: An Mr 200K/120K integrin heterodimer that binds laminin in a divalent cation-dependent manner
M. Ignatius (1988)
10.1016/0014-4827(85)90472-0
Involvement of plasma membrane glycoproteins in the contact-dependent inhibition of growth of human fibroblasts.
R. Wieser (1985)
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