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Chemical Strategies For Generating Protein Biochips.

P. Jonkheijm, Dirk Weinrich, H. Schröder, C. Niemeyer, H. Waldmann
Published 2008 · Chemistry, Medicine

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Protein biochips are at the heart of many medical and bioanalytical applications. Increasing interest has been focused on surface activation and subsequent functionalization strategies for immobilizing these biomolecules. Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function. Strategies for creating protein patterns (as opposed to protein arrays) are also outlined. An outlook on promising and challenging future directions for protein biochip research and applications is also offered.
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