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Integrated Fabrication‐conjugation Methods For Polymeric And Hybrid Microparticles For Programmable Drug Delivery And Biosensing Applications
Sukwon Jung, C. Choi, C. Lee, H. Yi
Published 2016 · Materials Science, Medicine
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Functionalized polymeric microparticles possess significant potential for controlled drug delivery and biosensing applications, yet current fabrication techniques face challenges in simple and scalable fabrication and biofunctionalization. For programmable manufacture of biofunctional microparticles in a simple manner, we have developed robust micromolding methods combined with biopolymeric conjugation handles and bioorthogonal click reactions. In this focused minireview, we present detailed methods for our integrated approaches for fabrication of microparticles with controlled 2D and 3D shapes and dimensions toward controlled release, and for biomacromolecular conjugation via strain promoted alkyne‐azide cycloaddition (SPAAC) and tetrazine‐trans‐cyclooctene (Tz‐TCO) ligation reactions utilizing a potent aminopolysaccharide chitosan as an efficient conjugation handle. We believe that the fabrication‐conjugation methods reported here from a range of our recent reports illustrate the simple, robust and readily reproducible nature of our approaches to creating multifaceted microparticles in a programmable, cost‐efficient and scalable manner toward a wide range of medical and biotechnological application areas.
This paper references
Multiplexed protein quantification with barcoded hydrogel microparticles.
D. Appleyard (2011)
Synthesis of monodispersed microspheres from laplace pressure induced droplets in micromolds.
C. Choi (2012)
Direct fabrication and harvesting of monodisperse, shape-specific nanobiomaterials.
J. Rolland (2005)
Hydrogel Biosensor Array Platform Indexed by Shape
Jason E. Meiring (2004)
Guided and fluidic self-assembly of microstructures using railed microfluidic channels.
S. E. Chung (2008)
Injectable hydrogel microbeads for fluorescence-based in vivo continuous glucose monitoring
H. Shibata (2010)
Droplet microfluidics for producing functional microparticles.
J. Kim (2014)
Shape-specific, monodisperse nano-molding of protein particles.
J. Y. Kelly (2008)
Polymeric systems for controlled drug release.
K. Uhrich (1999)
Integrated fabrication-conjugation approaches for biomolecular assembly and protein sensing with hybrid microparticle platforms and biofabrication - A focused minireview
Sukwon Jung (2015)
Click Chemistry in Materials Science
W. Xi (2014)
Fabrication of uniform DNA-conjugated hydrogel microparticles via replica molding for facile nucleic acid hybridization assays.
Christina L Lewis (2010)
Multiplexed hydrogel microparticle suspension arrays for facile ribosomal RNA integrity assays
Y. Duenas (2015)
Continuous-flow lithography for high-throughput microparticle synthesis
D. Dendukuri (2006)
Smart microgel capsules from macromolecular precursors.
S. Seiffert (2010)
The Synthesis and Assembly of Polymeric Microparticles Using Microfluidics
D. Dendukuri (2009)
Controlled Fabrication of Microparticles with Complex 3D Geometries by Tunable Interfacial Deformation of Confined Polymeric Fluids in 2D Micromolds.
C. Choi (2015)
Sequential assembly of cell‐laden hydrogel constructs to engineer vascular‐like microchannels
Y. Du (2011)
Surface-tension-induced synthesis of complex particles using confined polymeric fluids.
C. Choi (2010)
Controlled fabrication of multicompartmental polymeric microparticles by sequential micromolding via surface-tension-induced droplet formation.
C. Choi (2015)
Nanoemulsion composite microgels for orthogonal encapsulation and release.
H. An (2012)
Poly (N-isopropylacrylamide) Microgel-Based Optical Devices for Sensing and Biosensing
Molla R. Islam (2014)
Multiplexed Protein Quantification with Barcoded Hydrogel Microparticles Citation
D. Appleyard (2011)
Synthesis, characterization and antimicrobial activity of poly (N-vinyl imidazole) grafted carboxymethyl chitosan
M. Sabaa (2010)
Design of Asymmetric Particles Containing a Charged Interior and a Neutral Surface Charge: Comparative Study on in Vivo Circulation of Polyelectrolyte Microgels
K. Chen (2014)
Generation of monodisperse particles by using microfluidics: control over size, shape, and composition.
Shengqing Xu (2005)
Covalent immobilization of proteins on 3D poly(acrylic acid) brushes: mechanism study and a more effective and controllable process.
Zhenyuan Qu (2014)
Stop-flow lithography in a microfluidic device.
D. Dendukuri (2007)
Erratum: Generation of monodisperse particles by using microfluidics - Control over size, shape, and composition (Angewandte Chemie - International Edition (2005) 44 (724-728) DOI: 10.1002/anie.200462226)
Shengqing Xu (2005)
Synthesis, characterization and antimicrobial activity of poly(N-vinyl imidazole) grafted carboxymethyl chitosan. Carbohydr. Polym
M W Sabaa (2010)
Controllable preparation of monodisperse microspheres using geometrically mediated droplet formation in a single mold.
C. Choi (2013)
Fabrication of chitosan-poly(ethylene glycol) hybrid hydrogel microparticles via replica molding and its application toward facile conjugation of biomolecules.
Sukwon Jung (2012)
Controlled synthesis of nonspherical microparticles using microfluidics.
D. Dendukuri (2005)
An integrated approach for enhanced protein conjugation and capture with viral nanotemplates and hydrogel microparticle platforms via rapid bioorthogonal reactions.
Sukwon Jung (2014)
Spontaneous shape reconfigurations in multicompartmental microcylinders
K. Lee (2012)
Triblock cylinders at fluid-fluid interfaces.
Sung-min Kang (2014)
Facile Micromolding-Based Fabrication of Biopolymeric–Synthetic Hydrogel Microspheres with Controlled Structures for Improved Protein Conjugation
Sukwon Jung (2015)
Nanoimprint lithography based fabrication of shape-specific, enzymatically-triggered smart nanoparticles.
Luz Cristal S. Glangchai (2008)
Imparting size, shape, and composition control of materials for nanomedicine.
Larken E. Euliss (2006)
Dripping and jetting in microfluidic multiphase flows applied to particle and fiber synthesis.
J. Nunes (2013)
Multifunctional Encoded Particles for High-Throughput Biomolecule Analysis
D. Pregibon (2007)
Multifunctional photonic crystal barcodes from microfluidics
Yuanjin Zhao (2012)
Magnetoresponsive discoidal photonic crystals toward active color pigments.
H. S. Lee (2014)
Microengineered hydrogels for tissue engineering.
A. Khademhosseini (2007)
Optically diffracting hydrogels for screening kinase activity in vitro and in cell lysate: impact of material and solution properties.
Kelsey I. MacConaghy (2015)
Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning
D. Appleyard (2011)
Elaborate Design Strategies Toward Novel Microcarriers for Controlled Encapsulation and Release
Tae Soup Shim (2013)
Low modulus biomimetic microgel particles with high loading of hemoglobin.
K. Chen (2012)
Multiplexed detection of mRNA using porosity-tuned hydrogel microparticles.
Nak Won Choi (2012)
Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
Y. Du (2008)
Polymer‐based microparticles in tissue engineering and regenerative medicine
M. Oliveira (2011)
Facile strategy for protein conjugation with chitosan-poly(ethylene glycol) hybrid microparticle platforms via strain-promoted alkyne-azide cycloaddition (SPAAC) reaction.
Sukwon Jung (2013)
Facile strategy for protein conjugation with chitosan-poly(ethylene glycol) hybrid microparticle platforms via strainpromoted alkyne-azide cycloaddition (SPAAC) reaction. Biomacromolecules
S Jung (2013)
Controlled synthesis of 3D multi-compartmental particles with centrifuge-based microdroplet formation from a multi-barrelled capillary.
K. Maeda (2012)
Hydrogel microparticles for biosensing.
Gaelle C Le Goff (2015)
Chemically orthogonal three-patch microparticles.
S. Rahmani (2014)
Particle shape: a new design parameter for micro- and nanoscale drug delivery carriers.
J. Champion (2007)
A dynamic duo: pairing click chemistry and postpolymerization modification to design complex surfaces.
R. Arnold (2014)
Synthesis, characterization and metal uptake capacity of a new carboxymethyl chitosan derivative
I. ElSherbiny (2009)
Free radical damage to protein and DNA: Mechanisms involved and relevant observations on brain undergoing oxidative stress
R. Floyd (1992)
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M. Martínez-Ballesta (2018)
Customized hybrid and NIR-light triggered thermoresponsive drug delivery microparticles synthetized by photopolymerization in a one-step flow focusing continuous microreactor.
Isabel Ortiz de Solorzano (2020)
Improved Protein Conjugation with Uniform, Macroporous Poly(acrylamide-co-acrylic acid) Hydrogel Microspheres via EDC/NHS Chemistry.
E. Y. Liu (2016)
Correction in Bicinchoninic Acid (BCA) Absorbance Assay to Analyze Protein Concentration
D. J. Smith (2018)
High-throughput double emulsion-based microfluidic production of hydrogel microspheres with tunable chemical functionalities toward biomolecular conjugation.
E. Y. Liu (2018)