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Cell Targeting With Hybrid Qβ Virus‐Like Particles Displaying Epidermal Growth Factor
J. Pokorski, Marisa L. Hovlid, M. Finn
Published 2011 · Chemistry, Medicine
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Structurally uniform protein nanoparticles derived from the self‐assembly of viral capsid proteins are attractive platforms for the multivalent display of cell‐targeting motifs for use in nanomedicine. Virus‐based nanoparticles are of particular interest because the scaffold can be manipulated both genetically and chemically to simultaneously display targeting groups and carry a functional payload. Here, we displayed the human epidermal growth factor (EGF) on the exterior surface of bacteriophage Qβ as a C‐terminal genetic fusion to the Qβ capsid protein. The co‐assembly of wild‐type Qβ and EGF‐modified subunits resulted in structurally homogeneous nanoparticles displaying between 5 and 12 copies of EGF on their exterior surface. The particles were found to be amenable to bioconjugation by standard methods as well as the high‐fidelity copper‐catalyzed azide–alkyne cycloaddition reaction (CuAAC). Such chemical derivatization did not impair the ability of the particles to specifically interact with the EGF receptor. Additionally, the particle‐displayed EGF remained biologically active promoting autophosphorylation of the EGF receptor and apoptosis of A431 cells. These results suggest that hybrid Qβ‐EGF nanoparticles could be useful vehicles for targeted delivery of imaging and/or therapeutic agents.
This paper references
Epidermal growth factor inhibits growth of A431 human epidermoid carcinoma in serum-free cell culture
D. Barnes (1982)
Anti-EGFR monoclonal antibody in cancer treatment: in vitro and in vivo evidence
A. E. Quatrale (2011)
Folate-mediated Cell Uptake of Shell-crosslinked Spheres and Cylinders.
K. Zhang (2008)
RNA Phage Qβ Coat Protein as a Carrier for Foreign Epitopes
T. Kozlovska (1996)
Targeting vault nanoparticles to specific cell surface receptors.
V. Kickhoefer (2009)
Identification of the genetic locus for the structural gene and a new regulatory gene for the synthesis of repressible alkaline phosphatase in Saccharomyces cerevisiae.
Y. Kaneko (1982)
Cell Growth Differ
a L. Gulli (1996)
GeneDesign: rapid, automated design of multikilobase synthetic genes.
S. Richardson (2006)
Dual-surface modified virus capsids for targeted delivery of photodynamic agents to cancer cells.
N. Stephanopoulos (2010)
Molecular diagnostic and drug delivery agents based on aptamer-nanomaterial conjugates.
J. Lee (2010)
Engineering of vault nanocapsules with enzymatic and fluorescent properties.
V. Kickhoefer (2005)
A Viral Nanoparticle with Dual Function as an Anthrax Antitoxin and Vaccine
Darly J. Manayani (2007)
Development of targeted therapy with paclitaxel incorporated into EGF-conjugated nanoparticles.
T. Shimada (2009)
Heparin Antagonism by Polyvalent Display of Cationic Motifs on Virus‐Like Particles
A. Udit (2009)
Increased phosphotyrosine content and inhibition of proliferation in EGF-treated A431 cells
G. N. Gill (1981)
Knocking down barriers: advances in siRNA delivery
K. Whitehead (2009)
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells
A. Ullrich (1984)
Selection for thermostability can lead to the emergence of mutational robustness in an RNA virus
P. Domingo-Calap (2010)
Conjugation of peptides to the passivation shell of gold nanoparticles for targeting of cell-surface receptors.
Lisa Maus (2010)
Specific binding of baculoviruses displaying gp64 fusion proteins to mammalian cells.
K. Ojala (2001)
Protein cage nanoparticles bearing the LyP-1 peptide for enhanced imaging of macrophage-rich vascular lesions.
M. Uchida (2011)
Stabilization of virus-like particles with poly(2-oxazoline)s.
Florian Manzenrieder (2011)
Nat. Rev. Drug Disc.
D. Payne (2007)
B Liberelle (2010)
Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from Escherichia coli.
K. Sharma (2008)
Immunogenic display of diverse peptides on virus-like particles of RNA phage MS2.
D. Peabody (2008)
Antitumor activity of NRC-AN-019 in a pre-clinical breast cancer model.
C. Gondi (2011)
Integrated Multifunctional Nanosystems for Medical Diagnosis and Treatment
Donglu Shi (2009)
Assembly of hybrid bacteriophage Qbeta virus-like particles.
S. Brown (2009)
RNA-directed packaging of enzymes within virus-like particles.
Jason D. Fiedler (2010)
Alexa Dyes, a Series of New Fluorescent Dyes that Yield Exceptionally Bright, Photostable Conjugates
N. Panchuk-Voloshina (1999)
Internalization and down-regulation of the human epidermal growth factor receptor are regulated by the carboxyl-terminal tyrosines.
K. Helin (1991)
Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles.
Y. Vachutinsky (2011)
Mosaic Qβ coats as a new presentation model
I. Vasiljeva (1998)
Design and characterization of HER-2-targeted gold nanoparticles for enhanced X-radiation treatment of locally advanced breast cancer.
N. Chattopadhyay (2010)
Improved Display of Synthetic IgG-Binding Domains on the Baculovirus Surface
K. Ojala (2004)
Folate receptor-mediated drug targeting: from therapeutics to diagnostics.
A. Hilgenbrink (2005)
The crystal structure of bacteriophage Qβ at 3.5 å resolution
R. Golmohammadi (1996)
Engineering thermal stability in RNA phage capsids via disulphide bonds.
A. Ashcroft (2005)
Epidermal growth factor induces cell cycle arrest and apoptosis of squamous carcinoma cells through reduction of cell adhesion
L. Cao (2000)
40, 1114; b)
S Wan (2011)
Specific protein delivery to target cells by antibody-displaying bionanocapsules.
N. Kurata (2008)
Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.
A. Sorkin (1992)
Factors that control the circulation time of nanoparticles in blood: challenges, solutions and future prospects.
Jin-Wook Yoo (2010)
Targeting of epidermal growth factor receptor (EGFR)-expressing tumor cells with sterically stabilized affibody liposomes (SAL).
Julia Beuttler (2009)
Boronated epidermal growth factor as a potential targeting agent for boron neutron capture therapy of brain tumors.
J. Capala (1996)
Immobilization of bacteriophage Qbeta on metal-derivatized surfaces via polyvalent display of hexahistidine tags.
A. Udit (2008)
Engineering of self-assembled nanoparticle platform for precisely controlled combination drug therapy
Nagesh Kolishetti (2010)
Expression of Recombinant Human Epidermal Growth Factor in Escherichia coli and Characterization of its Biological Activity
A. F. Abdull Razis (2008)
Dendrimer-triglycine-EGF nanoparticles for tumor imaging and targeted nucleic acid and drug delivery.
Q. Yuan (2010)
Analysis and optimization of copper-catalyzed azide-alkyne cycloaddition for bioconjugation.
V. Hong (2009)
Targeted delivery for breast cancer therapy: the history of nanoparticle-albumin-bound paclitaxel
F. Petrelli (2010)
Targeted cellular delivery of quantum dots loaded on and in biotinylated liposomes.
V. Sigot (2010)
EGFR tyrosine kinases inhibitors in cancer treatment: in vitro and in vivo evidence.
A. E. Quatrale (2011)
Protein Expression Purif
A F Razis (2008)
Enhanced gene delivery by avidin-displaying baculovirus.
J. K. Räty (2004)
Multivalent Display and Receptor‐Mediated Endocytosis of Transferrin on Virus‐Like Particles
Deboshri Banerjee (2010)
Impact of epidermal growth factor tethering strategy on cellular response.
Benoît Liberelle (2010)
Epidermal growth factor-induced apoptosis in A431 cells can be reversed by reducing the tyrosine kinase activity.
L. F. Gulli (1996)
Targeted killing of cancer cells in vivo and in vitro with EGF-directed carbon nanotube-based drug delivery.
Ashwin Bhirde (2009)
Development of EGF-conjugated liposomes for targeted delivery of boronated DNA-binding agents.
Erika Bohl Kullberg (2002)
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Targeted delivery system for cancer cells consist of multiple ligands conjugated genetically modified CCMV capsid on doxorubicin GNPs complex
Indu Barwal (2016)
Integrating Display and Delivery Functionality with a Cell Penetrating Peptide Mimic as a Scaffold for Intracellular Multivalent Multitargeting.
Yugang Bai (2016)
Optimization of norovirus virus‐like particle production in Pichia pastoris using a real‐time near‐infrared bioprocess monitor
S. A. Parker (2016)
Development of Virus-Like Particle Technology from Small Highly Symmetric to Large Complex Virus-Like Particle Structures
P. Pushko (2013)
Biodegradable Viral Nanoparticle/Polymer Implants Prepared via Melt-Processing.
P. Lee (2017)
Protein nanoparticles for therapeutic protein delivery.
L. Herrera Estrada (2015)
Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting
M. Mie (2018)
Viral chemistry: the chemical functionalization of viral architectures to create new technology.
Zhuo Chen (2016)
Engineering Recombinant Virus-like Nanoparticles from Plants for Cellular Delivery.
Lou Brillault (2017)
Effective Delivery of Antigen-Encapsulin Nanoparticle Fusions to Dendritic Cells Leads to Antigen-Specific Cytotoxic T Cell Activation and Tumor Rejection.
Bongseo Choi (2016)
Plant viral nanoparticles for packaging and in vivo delivery of bioactive cargos.
M. Shahgolzari (2020)
Directed polyvalent display of sulfated ligands on virus nanoparticles elicits heparin-like anticoagulant activity.
G. Mead (2014)
Using FRET to measure the time it takes for a cell to destroy a virus.
Candace E Benjamin (2020)
Production and applications of engineered viral capsids
J. Glasgow (2014)
Programmed Self-Assembly of an Active P22-Cas9 Nanocarrier System.
Shefah Qazi (2016)
Quantum Dots Encapsulated with Canine Parvovirus-Like Particles Improving the Cellular Targeted Labeling
D. Yan (2015)
Bioengineering Strategies for Protein-Based Nanoparticles
D. Diaz (2018)
Multivalent Viral Capsids with Internal Cargo for Fibrin Imaging
Allie C. Obermeyer (2014)
Developing genetically engineered encapsulin protein cage nanoparticles as a targeted delivery nanoplatform.
H. Moon (2014)
Caged protein nanoparticles for drug delivery.
Nicholas M. Molino (2014)
A Target-Specific Oral Formulation of Doxorubicin-Protein Nanoparticles: Efficacy and Safety in Hepatocellular Cancer
Kishore Golla (2013)
Alaa A A Aljabali (2013)
The True Story and Advantages of RNA Phage Capsids as Nanotools
P. Pumpens (2016)
"Graft-to" Protein/Polymer Conjugates Using Polynorbornene Block Copolymers.
Sergey A. Isarov (2016)
In vivo virus-based macrofluorogenic probes target azide-labeled surface glycans in MCF-7 breast cancer cells.
Clorissa L Washington-Hughes (2013)
Bioengineered protein-based nanocage for drug delivery.
E. Lee (2016)
Reengineering viruses and virus-like particles through chemical functionalization strategies.
M. Smith (2013)
Intracellular Delivery II
A. Prokop (2014)
Design, display and immunogenicity of HIV1 gp120 fragment immunogens on virus-like particles.
Mansi Purwar (2018)
In Vitro Assembly of Virus-Derived Designer Shells Around Inorganic Nanoparticles.
S. Vieweger (2018)
Photodynamic activity of viral nanoparticles conjugated with C60.
A. Wen (2012)
Multifunctional Protein-Based Nanoparticles for Cancer Theranosis
L. Vannucci (2014)See more