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Development Of An Amperometric Enzymatic Biosensor Based On Gold Modified Magnetic Nanoporous Microparticles

A. El-Yacoubi, S. I. Zayed, B. Blankert, J. Kauffmann
Published 2006 · Chemistry

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Gold modified nanoporous silica based magnetic microparticles have been prepared as support for the immobilization of the enzyme horseradish peroxidase (HRP). The enzyme modified gold microparticles were retained onto the surface of a solid carbon paste electrode with the help of a permanent magnet. The analytical performances of the resulting biosensor were characterized by studying hydroquinone (HQ) and hydrogen peroxide. The former was monitored by the direct electroreduction of the biocatalytically generated quinone. Several experimental parameters influencing the biosensor response were investigated. A linear response to HQ was obtained in the concentration range comprised between 5×10−7 and 4.5×10−6 M with a detection limit of 4×10−7 M. The enzyme electrode provided a linear response to hydrogen peroxide over a concentration range comprised between 5×10−7−1.3×10−4 M with a detection limit of 4×10−7 M. The inhibition of the biosensor response in the presence of thiols e.g. cysteine, captopril, glutathione and Nacystelyn (NAL) has been pointed out.
This paper references
10.1016/S0003-2670(03)00765-7
A mechanistic evaluation of the amperometric response of reduced thiols in quinone mediated systems
C. G. Stone (2003)
10.1016/S0304-8853(00)01243-9
Polyvinyl-alcohol-based magnetic beads for rapid and efficient separation of specific or unspecific nucleic acid sequences
J. Oster (2001)
10.1080/00032719208020039
Comparative Studies of Immobilized Enzyme Electrodes Based on the Inhibitory Effect of Nicotine on Choline Oxidase and Acetylcholinesterase
T. Nwosu (1992)
10.1016/S0003-2670(03)00725-6
Nanoparticle-based electrochemical DNA detection
J. Wang (2003)
10.1021/AC0104680
Flow-based microimmunoassay.
M. Hayes (2001)
10.1016/S0731-7085(03)00562-4
Detection methods of microsphere based single-step bioaffinity and in vitro diagnostics assays.
J. Soini (2004)
10.1016/J.CES.2005.05.067
Nanostructures for enzyme stabilization
Jungbae Kim (2006)
10.1016/J.ACA.2004.10.077
A phenol biosensor based on immobilizing tyrosinase to modified core-shell magnetic nanoparticles supported at a carbon paste electrode
Z. Liu (2005)
10.1016/S0021-9673(97)00629-8
Automated microanalysis using magnetic beads with commercial capillary electrophoretic instrumentation.
L. Rashkovetsky (1997)
10.1016/S1381-1177(96)00017-3
Enzyme immobilization in MCM-41 molecular sieve
J. F. Diaz (1996)
10.1016/J.JELECHEM.2004.03.029
On-line cysteine modification for protein analysis: new probes for electrochemical tagging nanospray mass spectrometry
C. Roussel (2004)
10.1021/ja00741a013
Cyanohydridoborate anion as a selective reducing agent
R. Borch (1971)
10.1021/AC049489V
Preparation, characterization, and application of an enzyme-immobilized magnetic microreactor for flow injection analysis.
A. Nomura (2004)
10.1016/J.SNB.2004.08.023
A hydrogen peroxide biosensor based on nano-Au/PAMAM dendrimer/cystamine modified gold electrode
Z. Liu (2005)
10.1016/S0168-1605(00)00262-2
Immunomagnetic separation of a Norwalk-like virus (genogroup I) in artificially contaminated environmental water samples.
M. Myrmel (2000)
10.1002/ELAN.200503266
Enhancement of Bioelectrocatalytic Processes by the Rotation of Mediator-Functionalized Magnetic Particles on Electrode Surfaces: Comparison with a Rotating Disk Electrode
Eugenii Katz (2005)
10.1016/J.COLSURFA.2004.04.048
Synthesis and characterization of nanoporous silica support for enzyme immobilization
Jong-kil Kim (2004)
10.1016/J.APSUSC.2004.06.080
Functionalized nanoporous silicas for the immobilization of penicillin acylase
A. Chong (2004)
10.1016/0024-3205(93)90107-E
Enzymatic formation and electrochemical characterization of multiply substituted glutathione conjugates of hydroquinone.
D. L. Puckett-Vaughn (1993)
10.1016/S0039-9140(02)00641-0
Immobilization of horseradish peroxidase to a nano-Au monolayer modified chitosan-entrapped carbon paste electrode for the detection of hydrogen peroxide.
Cun-xi Lei (2003)
10.1006/JCIS.1995.1137
Cell Separation Using Protein-A-Coated Magnetic Nanoclusters
S. Sonti (1995)
10.1007/s002620050531
Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads
C. Petrarca (1999)
10.1016/J.ACA.2005.01.073
A disposable immunomagnetic electrochemical sensor based on functionalised magnetic beads and carbon-based screen-printed electrodes (SPCEs) for the detection of polychlorinated biphenyls (PCBs)
S. Centi (2005)
10.1016/0003-9861(77)90261-2
Proteins containing reductively aminated disaccharides. Synthesis and chemical characterization.
B. Schwartz (1977)
10.1021/AC035503C
Bead-based electrochemical immunoassay for bacteriophage MS2.
J. H. Thomas (2004)
10.1063/1.1801158
Morphologic evolution and optical properties of nanostructured gold based on mesoporous silica
C. Kan (2004)
10.1038/nbt931
Enzyme immobilization in a biomimetic silica support
H. Luckarift (2004)
10.1016/S1388-2481(02)00311-9
Magnetic-field stimulated DNA oxidation
J. Wang (2002)
10.1021/AC970441+
Flow injection immunoanalysis based on a magnetoimmunosensor system.
S. Solé (1998)
10.1016/0039-9140(95)01645-7
Preparation and characterization of a new enzyme electrode based on solid paraffin and activated graphite particles.
C. Petit (1995)
10.1021/ac00037a010
Sequential injection immunoassay utilizing immunomagnetic beads.
C. Pollema (1992)
10.1016/J.ACA.2005.06.048
Novel separation and preconcentration of trace amounts of copper(II) in water samples based on neocuproine modified magnetic microparticles
P. Ashtari (2005)
10.1039/B107540N
An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities.
Jin-Woo Choi (2002)



This paper is referenced by
10.1016/j.aca.2010.06.043
Magnetic solids in analytical chemistry: a review.
K. Aguilar-Arteaga (2010)
10.1016/J.SNB.2008.09.054
Selective analysis of reduced thiols with a novel bionanomultilayer biosensor based on the inhibition principle
Lijun Liu (2009)
10.1016/J.BIOS.2006.11.013
Development of amperometric horseradish peroxidase based biosensors for clozapine and for the screening of thiol compounds.
Donghui Yu (2007)
10.20450/MJCCE.2018.1442
Covalent attachment of phenyl and carboxyphenyl layers derived from diazonium salts onto activated charcoal for the adsorption of pesticides
J. Halili (2018)
10.1016/j.aca.2014.11.008
Chemically glycosylation improves the stability of an amperometric horseradish peroxidase biosensor.
Griselle Hernández-Cancel (2015)
10.3390/S7040589
Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads
N. Jaffrezic-Renault (2007)
10.1016/j.bios.2013.04.016
Facile fabrication of AgNPs/(PVA/PEI) nanofibers: high electrochemical efficiency and durability for biosensors.
H. Zhu (2013)
10.1016/j.microc.2019.104282
Evaluation of phenolic antioxidant capacity in beverages based on laccase immobilized on screen-printed carbon electrode modified with graphene nanoplatelets and gold nanoparticles
Ivana Zrinski (2020)
10.1080/10408340903011853
Recent Advances in Electroanalysis of Organic Compounds at Carbon Paste Electrodes
Jiří Zíma (2009)
10.1016/J.JELECHEM.2012.06.004
Glass carbon electrode modified with horseradish peroxidase immobilized on partially reduced graphene oxide for detecting phenolic compounds
Yan Zhang (2012)
10.2478/s11532-010-0070-7
Screen-printed electrodes modified with HRP-zirconium alcoxide film for the development of a biosensor for acetaminophen detection
Veronica Harceaga Sima (2010)
10.1016/j.bios.2011.02.019
Improved bacteria detection by coupling magneto-immunocapture and amperometry at flow-channel microband electrodes.
O. Laczka (2011)
DEVELOPMENT OF ELECTROCHEMICAL DEVICES FOR THE DETERMINATION OF DRUGS OF ABUSE
Departamento de Engenharia Química (2014)
10.1016/j.bios.2016.01.026
Selective determination of phenols and aromatic amines based on horseradish peroxidase-nanoporous gold co-catalytic strategy.
Chao Wu (2016)
10.1007/978-1-4419-9622-0_6
Biosensors Based on Nanoporous Materials
H. Ju (2011)
10.1016/J.SNB.2017.06.001
Construction of a biosensor based on SAM of thiolactic acid on gold nanoparticles stabilized by silsesquioxane polyelectrolyte for cathecol determination
Rosana Mossanha (2017)
10.1080/00032719.2013.867499
Laccase Biosensor Based on Graphene-Chitosan Composite Film for Determination of Hydroquinone
Jianying Qu (2014)
10.1002/ELAN.200603579
A Peroxidase-Based Biosensor Supported by Nanoporous Magnetic Silica Microparticles for Acetaminophen Biotransformation and Inhibition Studies
Donghui Yu (2006)
10.1149/2.1191810JES
Ultrasensitive Amperometric Biosensing of Polyphenols Using Horseradish Peroxidase Immobilized in a Laponite/Au/DNA-Bioinspired Polycation Nanocomposite
Fiorella Tulli (2018)
10.5772/7201
Nanostructured Metal Oxides Based Enzymatic Electrochemical Biosensors
A. Ansari (2010)
10.2116/ANALSCI.27.535
Differential pulse anodic voltammetric determination of tiapride hydrochloride in pharmaceutical preparation and human urine using carbon paste electrodes.
Sayed I. M. Zayed (2011)
10.1016/J.SNB.2012.07.054
Gold nanoparticles dispersed in zwitterionic surfactant for peroxidase immobilization in biosensor construction
S. Fernandes (2012)
10.1016/j.bios.2009.10.014
High-performance glucose amperometric biosensor based on magnetic polymeric bionanocomposites.
Can Zou (2010)
10.1007/s13738-020-01962-7
New and sensitive magnetic carbon paste electrode for voltammetry determination of morphine and methadone
Nahid Yousefi (2020)
10.1016/J.MICROC.2018.12.055
Simultaneous determination of antinonin and trifluralin by electrochemical method and net analyte signal interferent modeling
Maryam Haghighi (2019)
NEW MODIFIED ELECTRODES WITH HRP IMMOBILIZED IN POLYMERIC FILMS FOR PARACETAMOL ANALYSIS
Andreea Cernat (2012)
10.1016/J.COLSURFA.2018.09.065
Preparation of polyaniline-encapsulated carbon/copper composite nanofibers for detection of polyphenol pollutant
Y. Fu (2018)
10.1016/j.bios.2010.09.046
Conductive polymer-based sensors for biomedical applications.
S. Nambiar (2011)
10.1016/J.BIOS.2007.02.018
A highly sensitive biosensor with (Con A/HRP)n multilayer films based on layer-by-layer technique for the detection of reduced thiols.
L. Liu (2007)
Recent Advances in Electroanalysis of Organic Compounds at Carbon Paste Electrodes
Karel Vyt (2009)
10.1021/acs.biomac.5b00768
Temperature-Invariant Aqueous Microgels as Hosts for Biomacromolecules.
Sepehr Mastour Tehrani (2015)
10.1016/J.COLSURFA.2017.07.003
Facile preparation of gold-coated polydimethylsiloxane particles by in situ reduction without pre-synthesized seed
Min-Cheol Lim (2017)
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