Online citations, reference lists, and bibliographies.

Meiotic Arrest And Spindle Defects Are Associated With Altered KIF11 Expression In Porcine Oocytes

Xiang Wan, Y. Zhang, M. Lan, Meng-Hao Pan, F. Tang, Hao-lin Zhang, Xianghong Ou, S. Sun
Published 2018 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Kinesin superfamily proteins (KIFs) act as molecular motors and are involved in material transport along microtubules to maintain normal cellular functions. KIF11 (also named kinesin‐5, Eg5, and KSP) is a plus‐end‐directed homotetrameric kinesin that regulates spindle formation for actuate chromosomal separation during mitosis. However, the roles of KIF11 in meiosis are still unclear. In this study, we investigated the regulatory functions of KIF11 during porcine oocyte maturation. The results indicated that KIF11 was expressed in different stages during porcine oocyte meiosis. Inhibition of KIF11 activity led to the failure of the first polar body extrusion, and we found that cell cycle progression was disturbed, which was confirmed by the decreased Cdc2 expression. Furthermore, inhibition of KIF11 resulted in decreased tubulin acetylation and caused sequential disruption of the spindle assembly and chromosome alignment. We also found that in postovulatory aging porcine oocytes, the KIF11 expression was altered, indicating that KIF11 was involved with aging‐induced spindle disorganization. In summary, our results showed that KIF11 regulated the cell cycle and tubulin acetylation related spindle formation in porcine oocyte meiosis. Environ. Mol. Mutagen. 59:805–812, 2018. © 2018 Wiley Periodicals, Inc.
This paper references
Molecular motors and mechanisms of directional transport in neurons
N. Hirokawa (2005)
Prophase I arrest and progression to metaphase I in mouse oocytes: comparison of resumption of meiosis and recovery from G2-arrest in somatic cells.
P. Solc (2010)
Mitotic spindle organization by a plus-end-directed microtubule motor
K. Sawin (1992)
Microtubules containing acetylated alpha-tubulin in mammalian cells in culture
G. Piperno (1987)
Posttranslational modifications of alpha tubulin: detyrosination and acetylation differentiate populations of interphase microtubules in cultured cells
J. C. Bulinski (1988)
Silencing of KIF14 interferes with cell cycle progression and cytokinesis by blocking the p27Kip1 ubiquitination pathway in hepatocellular carcinoma
Haidong Xu (2014)
Organelle transport along microtubules - the role of KIFs.
N. Hirokawa (1996)
Microtubule-associated histone deacetylase 6 supports the calcium store sensor STIM1 in mediating malignant cell behaviors.
Yanxian Chen (2013)
Kinesin and dynein superfamily proteins and the mechanism of organelle transport.
N. Hirokawa (1998)
Eg5 inhibitor YL001 induces mitotic arrest and inhibits tumor proliferation
Y. Wang (2017)
MKlp2 inhibitior paprotrain affects polar body extrusion during mouse oocyte maturation
Jun Liu (2013)
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
N. Furuno (1994)
KIF7 Controls the Proliferation of Cells of the Respiratory Airway through Distinct Microtubule Dependent Mechanisms
Garry L. Coles (2015)
Inhibition of KSP by ARRY-520 induces cell cycle block and cell death via the mitochondrial pathway in AML cells
B. Carter (2009)
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs
J. Gerhart (1984)
Analysis of the kinesin superfamily: insights into structure and function.
H. Miki (2005)
The novel kinesin spindle protein (KSP) inhibitor SB-743921 exhibits marked activity in in vivo and in vitro models of aggressive large B-cell lymphoma
Danielle C Bongero (2015)
Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo
Anne Slangy (1995)
Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis.
Changjun Zhu (2005)
Kinesin-5, a mitotic microtubule-associated motor protein, modulates neuronal migration
Aditi Falnikar (2011)
Opposing motor activities are required for the organization of the mammalian mitotic spindle pole
T. Gaglio (1996)
The cohesion establishment factor Esco1 acetylates alpha-tubulin
Y Lu (2018)
Molecular mechanisms of kinesin-14 motors in spindle assembly and chromosome segregation
Zhen-Yu She (2017)
The cohesion establishment factor Esco1 acetylates α-tubulin to ensure proper spindle assembly in oocyte meiosis
Yajuan Lu (2018)
Microtubules acquire resistance from mechanical breakage through intralumenal acetylation
Zhenjie Xu (2017)
Spindle assembly checkpoint and its regulators in meiosis.
S. Sun (2012)
Post-translational modifications of tubulin and the dynamics of microtubules in mouse oocytes and zygotes.
H. de Pennart (1988)
Mechanisms of Mitotic Spindle Assembly.
S. Petry (2016)
KIF11 silencing and inhibition induces chromosome instability that may contribute to cancer
Yasamin Asbaghi (2017)
Geometry and force behind kinetochore orientation: lessons from meiosis
Y. Watanabe (2012)
Turning it on and off: M-phase promoting factor during meiotic maturation and fertilization.
K. Jones (2004)
The role of kinesin KIF18A in the invasion and metastasis of hepatocellular carcinoma
W. Luo (2018)
KSP inhibitor SB743921 induces death of multiple myeloma cells via inhibition of the NF-κB signaling pathway
I. Song (2015)
The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks
L. Kapitein (2005)
Age-Associated Dysregulation of Protein Metabolism in the Mammalian Oocyte
Francesca E Duncan (2018)
Survivin regulates Plk1 localization to kinetochore in mouse oocyte meiosis.
S. Sun (2012)
Protein Phosphatase 2A (PP2A) Regulates EG5 to Control Mitotic Progression
Y. Liu (2017)
Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility.
Y. Miao (2009)
Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes
F. Tang (2018)
KSP inhibitor SB743921 inhibits growth and induces apoptosis of breast cancer cells by regulating p53, Bcl-2, and DTL
Li Zhu (2016)
Advances in the discovery of kinesin spindle protein (Eg5) inhibitors as antitumor agents.
H. B. El-Nassan (2013)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar