Toxicity Of Cobalt-chromium Nanoparticles Released From A Resurfacing Hip Implant And Cobalt Ions On Primary Human Lymphocytes In Vitro.
Olga M. Posada, Rothwelle J. Tate, Mary Helen Grant
Published 2015 · Medicine, Chemistry
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Adverse tissue responses to prostheses wear particles and released ions are important contributors to hip implant failure. In implant-related adverse reactions T-lymphocytes play a prominent role in sustaining the chronic inflammatory response. To further understand the involvement of lymphocytes in metal-on-metal (MoM) implant failure, primary human lymphocytes were isolated and treated with cobalt-chromium (Co-Cr) wear debris and Co ions, individually, and in combination, for 24, 48 and 120 h. There was a significant increase in cell number where debris was present, as measured by the Neutral Red assay. Interleukin-6 (IL-6), interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) secretion levels significantly decreased in the presence of metal particles, as measured by ELISA. Interleukin-2 (IL-2) secretion levels were significantly decreased by both debris and Co ions. Flow cytometry analysis showed that the metal nanoparticles induced a significant increase in apoptosis after 48-h exposure. This investigation showed that prolonged exposure (120 h) to metal debris induces lymphocyte proliferation, suggesting that activation of resting lymphocytes may have occurred. Although cytokine production was affected mainly by metal debris, cobalt toxicity may also modulate IL-2 secretion, and even Co ion concentrations below the MHRA guideline levels (7 ppb) may contribute to the impairment of immune regulation in vivo in patients with MoM implants.
This paper references
Cobalt from metal-on-metal hip replacements may be the clinically relevant active agent responsible for periprosthetic tissue reactions.
Alister J. Hart (2012)
Local and systemic toxicity of nanoscale debris particles in total hip arthroplasty.
Ioannis Polyzois (2012)
New Insights into the Molecular Basis of T Cell Anergy: Anergy Factors, Avoidance Sensors, and Epigenetic Imprinting
Andrew D. Wells (2009)
Background of metal-on-metal resurfacing
Harlan C. Amstutz (2006)
Analysis of lymphocyte proliferation by cytofluorometric techniques in aging and various clinical conditions.
Alain L. de Weck (1984)
A benign psoas mass following metal-on-metal resurfacing of the hip.
D. Boardman (2006)
An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow.
Manfred B Lutz (1999)
Polyneuropathy caused by cobalt-chromium metallosis after total hip replacement.
Tokuhei Ikeda (2010)
Metal is not inert: role of metal ions released by biocorrosion in aseptic loosening--current concepts.
Dieter Cadosch (2009)
Cytidine deaminase activity in synovial fluid of patients with rheumatoid arthritis: relation to lactoferrin, acidosis, and cartilage proteoglycan release.
Bengt Månsson (1990)
Histological Features of Pseudotumor-like Tissues From Metal-on-Metal Hips
Patricia A Campbell (2010)
Cytokine secretion from human peripheral blood mononuclear cells cultured in vitro with metal particles.
Sandra C. P. Cachinho (2013)
Analysis of metal ion-induced DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni2+ and V3+ are more toxic than other metals: Al3+, Be2+, Co2+, Cr3+, Cu2+, Fe3+, Mo5+, Nb5+, Zr2+.
Marco S. Caicedo (2008)
Acute inflammatory response to cobalt chromium orthopaedic wear debris in a rodent air-pouch model
Moeed Akbar (2012)
Distribution of metal released from cobalt-chromium alloy orthopaedic wear particles implanted into air pouches in mice.
Grace A Afolaranmi (2012)
Arthroprosthetic cobaltism and cardiomyopathy.
Colin Machado (2012)
Dominance of the BV17 element in nickel-specific human T cell receptors relates to severity of contact sensitivity.
Joerg Vollmer (1997)
Analysis of wear of retrieved metal-on-metal hip resurfacing implants revised due to pseudotumours.
Y-M. Kwon (2010)
A systematic review of modern metal-on-metal total hip resurfacing vs standard total hip arthroplasty in active young patients.
Yonghua Jiang (2011)
Human leptin enhances activation and proliferation of human circulating T lymphocytes.
Consuelo Martín-Romero (2000)
Immunomodulation by metals.
David A. Lawrence (2002)
T Lymphocyte-Mediated Immune Responses to Chemical Haptens and Metal Ions: Implications for Allergic and Autoimmune Disease
Stefan F Martin (2004)
Characterisation of wear particles produced by metal on metal and ceramic on metal hip prostheses under standard and microseparation simulation
Christopher Mark Brown (2007)
Severe cobalt intoxication due to prosthesis wear in repeated total hip arthroplasty.
Marcus Oldenburg (2009)
Activation and proliferation of normal resting human T lymphocytes in serum-free culture: role of IL-4 and IL-6.
D. Habetswallner (1988)
Production of TNF-alpha and bone resorbing activity by macrophages in response to different types of bone cement particles.
Eileen Ingham (2000)
Consequences of exposure to peri-articular injections of micro- and nano-particulate cobalt-chromium alloy.
Christopher Mark Brown (2013)
Differential lymphocyte reactivity to serum-derived metal-protein complexes produced from cobalt-based and titanium-based implant alloy degradation.
Nadim James Hallab (2001)
Toxicology-based cancer causation analysis of CoCr-containing hip implants: a quantitative assessment of genotoxicity and tumorigenicity studies.
Whitney V. Christian (2014)
Loss of sight and sound. Could it be the hip?
Maria Cristina Rizzetti (2009)
Effect of bearing size on the long-term wear, wear debris, and ion levels of large diameter metal-on-metal hip replacements-An in vitro study.
Ian J Leslie (2008)
Massive metallosis after revision of a fractured ceramic head onto a metal head
Georg Matziolis (2002)
TCR reactivity in human nickel allergy indicates contacts with complementarity-determining region 3 but excludes superantigen-like recognition.
Joerg Vollmer (1999)
Arthroprosthetic cobaltism: neurological and cardiac manifestations in two patients with metal-on-metal arthroplasty: a case report.
Stephen S. Tower (2010)
Metal wear particle characterization from metal on metal total hip replacements: transmission electron microscopy study of periprosthetic tissues and isolated particles.
P F Doorn (1998)
Severe cobalt intoxication following hip replacement revision: Clinical features and outcome
Daniela Pelclova (2012)
A review of metallic, ceramic and surface-treated metals used for bearing surfaces in human joint replacements
P. A. Dearnley (1999)
Effect of cobalt and chromium ions on MMP-1, TIMP-1, and TNF-alpha gene expression in human U937 macrophages: a role for tyrosine kinases.
Li Luo (2005)
Metal-on-metal bearings and hypersensitivity in patients with artificial hip joints. A clinical and histomorphological study.
Hans-Georg Prof. Dr.-Med. Willert (2005)
Hip Resurfacing Data from National Joint Registries: What Do They Tell Us? What Do They Not Tell Us?
Kristoff Corten (2010)
Titanium, chromium and cobalt ions modulate the release of bone-associated cytokines by human monocytes/macrophages in vitro.
J Y Wang (1996)
Cytokine Production by Human Peripheral Blood Mononuclear cells after Exposure to Heavy Metals
Beatríz María (2000)
Metal-on-metal hip resurfacing: advantages and disadvantages.
Mario J. Quesada (2008)
Pseudotumours associated with metal-on-metal hip resurfacings.
Hemant Pandit (2008)
Metallosis after contemporary metal-on-metal total hip arthroplasty. Five to nine-year follow-up.
Panagiotis Korovessis (2006)
Effect of chromium and cobalt ions on primary human lymphocytes in vitro
Moeed Akbar (2011)
Necrotic and inflammatory changes in metal-on-metal resurfacing hip arthroplasties
Gayana Mahendra (2009)
Characterization of metal-wear nanoparticles in pseudotumor following metal-on-metal hip resurfacing.
Zhidao Xia (2011)
Severe cobalt poisoning with loss of sight after ceramic-metal pairing in a hip—a case report
Wolfram Steens (2006)
An unusual lymphocytic perivascular infiltration in tissues around contemporary metal-on-metal joint replacements.
A. Paul Davies (2005)
Molecular regulation of T-cell anergy.
Yan Zheng (2008)
Comparison of Alloys and Designs in a Hip Simulator Study of Metal on Metal Implants
John B. Medley (1996)
The role of the TH1 and TH2 immune responses in loosening and osteolysis of cemented total hip replacements.
Amarpal S Arora (2003)
CoCr wear particles generated from CoCr alloy metal-on-metal hip replacements, and cobalt ions stimulate apoptosis and expression of general toxicology-related genes in monocyte-like U937 cells.
Olga M. Posada (2014)
Adverse reaction to metal debris following hip resurfacing: the influence of component type, orientation and volumetric wear.
David John Langton (2011)
Induction of T cell anergy: integration of environmental cues and infectious tolerance.
Pascal Chappert (2010)
Postmortem study of femoral osteolysis associated with metal-on-metal articulation in total hip replacement: an analysis of nine cases.
Monika Huber (2010)
Corrosion degradation and prevention by surface modification of biometallic materials
Raghuvir Singh (2007)
Metal ion release from metal implants
Takao Hanawa (2004)
Metal sensitivity in patients with orthopaedic implants.
Nadim James Hallab (2001)
Molecular mechanisms of T-cell anergy
E. M. Kuklina (2013)
Activated lymphocytes as a metabolic model for carcinogenesis
Andrew N. Macintyre (2013)
This paper is referenced by
The Local and Systemic Effects of Cobalt-Chromium Nanoparticles on the Human Body: The Implications for Metal-on-Metal Hip Arthroplasty
James Drummond (2017)
Role of Nanotechnology in Cosmeceuticals: A Review of Recent Advances
Shreya Kaul (2018)
Ionic cobalt but not metal particles induces ROS generation in immune cells in vitro.
Kathrin Chamaon (2019)
Dual properties of zirconia coated porous titanium for a stiffness enhanced bio-scaffold
Han Lee (2017)
Local Biological Reactions and Pseudotumor-Like Tissue Formation in relation to Metal Wear in a Murine In Vivo Model
Alexander Paulus (2019)
Biological Reactions to Metal Particles and Ions in the Synovial Layer of Mice
Xiangyun Cheng (2020)
Immunotoxicology of Metals
Mitchell D. Cohen (2018)
Influence of Cobalt Ions on Collagen Gel Formation and Their Interaction with Osteoblasts
Emma M. McCarthy (2018)
Comparison of Patient-Reported Outcome from Neck-Preserving, Short-Stem Arthroplasty and Resurfacing Arthroplasty in Younger Osteoarthritis Patients
Marius Dettmer (2015)
Current Awareness in Clinical Toxicology
Damian Ballam (2013)
Effects of Endoprosthesis Head Material on Acetabular Cartilage Metabolism: An Animal Study Using Crossbred Pigs.
Shuhei Matsui (2018)
SMART Biosensor for Early Diagnostic Detection of Metal Ion Release in Orthopedic Patients: Initial Outcome
Mathew T. Mathew (2018)
Differential toxicity of processed and non-processed states of CoCrMo degradation products generated from a hip simulator on neural cells
Divya Rani Bijukumar (2018)
Diagnosis and management of implant debris-associated inflammation
Stuart B Goodman (2019)
Mechanisms of Adverse Local Tissue Reactions to Hip Implants
Felipe Eltit (2019)
Biotribocorrosion of Implants
Magdalena Walczak (2016)
Patch Testing for Evaluation of Hypersensitivity to Implanted Metal Devices: A Perspective From the American Contact Dermatitis Society
Peter C. Schalock (2016)
Effects of Corroded and Non-Corroded Biodegradable Mg and Mg Alloys on Viability, Morphology and Differentiation of MC3T3-E1 Cells Elicited by Direct Cell/Material Interaction
Sepideh Mostofi (2016)
Exploring the Incidence, Implications, and Relevance of Metal Allergy to Orthopaedic Surgeons
Stefanos F. Haddad (2019)
Is Metal-On-Metal Total Hip Arthroplasty Associated With Neurotoxicity?
Abiram Bala (2016)
Metal‐on‐metal total hip arthroplasty is not associated with cardiac disease
L. Henry Goodnough (2018)