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Reduction Of Hexavalent Chromium By A Novel Ochrobactrum Sp. – Microbial Characteristics And Reduction Kinetics
M. Narayani, K. Vidya Shetty
Published 2014 · Chemistry, Medicine
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A Gram negative hexavalent chromium (Cr(VI)) reducing bacteria, Ochrobactrum sp. Cr‐B4 (genbank accession number: JF824998) was isolated from the aerator water of an activated sludge process of a wastewater treatment facility of a dye and pigment based specialty chemical industry. It showed a resistance for 1000 mg L−1 Cr(VI). It exhibited resistance against other heavy metal ions like Ni2+ (900 mg L−1), Cu2+ (500 mg L−1), Pb2+ (800 mg L−1), and Cd2+(250 mg L−1), Zn2+ (700 mg L−1), Fe3+ (800 mg L−1), and against selected antibiotics. Cr‐B4 could efficiently reduce 200 mg L−1 Cr(VI) completely in nutrient and LB media and could convert Cr(VI) to Cr(III) efficiently. Cr(VI) reduction in nutrient media followed allosteric enzyme kinetics with Km values of 59.39 mg L−1 and Vmax values of 47.03 mg L−1 h−1. The reduction in LB media followed Michaelis–Menten kinetics with Km values of 99.52 mg L−1 and Vmax of 77.63 mg L−1 h−1. Scanning electron micrograms revealed the presence of extracellular polymeric secretions.
This paper references
Reduction of hexavalent chromium by Streptomyces griseus
R. Laxman (2002)
Volatilization of mercury and organomercurials determined by F factor system in enteric bacilli
L Schottel (1974)
Bergey's Manual of Determinative Bacteriology
S. T. Cowan (1948)
Reduction of Toxic Chromium and Partial Localization of Chromium Reductase Activity in Bacterial Isolate DM1
Urvashi Thacker (2005)
High levels of multiple metal resistance and its correlation to antibiotic resistance in environmental isolates of Acinetobacter
P. Dhakephalkar (2004)
Comparative study of Cr(VI) uptake and reduction in industrial effluent by Ochrobactrum intermedium and Brevibacterium sp.
M. Faisal (2004)
A comparative study of various biosorbents for removal of chromium (e) ions from industrial wastewater
M. Nourbakhsh (1994)
Volatilization of mercury and organomercurials determined 304
L. Schottel (1974)
Effects of organic amendments on the reduction and phytoavailability of chromate in mineral soil.
N. Bolan (2003)
Bioremoval of hexavalent chromium from water by a salt tolerant bacterium, Exiguobacterium sp. GS1
B. Okeke (2008)
A systematic study of chromium solubility in the presence of organic matter: consequences for the treatment of chromium-containing wastewater
E. Remoundaki (2007)
PLASMID MEDIATED CO-TRANSFER OF ANTIBIOTIC RESISTANCE AND HEAVY METAL TOLERANCE IN COLI FORMS
P. Ramteke (1997)
Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals.
S. Sultan (2007)
Chromate Resistance and Reduction by Bacterial Isolates
E. Parameswari (2009)
Plasmid-linked resistance to inorganic salts in Staphylococcus aureus.
R. Novick (1968)
Ochrobactrum tritici strain 5bvl1 - characterization of a Cr(VI)-resistant and Cr(VI)-reducing strain.
Rita Branco (2004)
Restriction enzyme analysis of 16S rRNA genes for the rapid identification of Thiobacillus ferrooxidans, Thiobacillus thiooxidans and Leptospirillum ferrooxidans strains in leaching environments
D. E. Rawlings (1995)
Volatilisation of mercury and organomercurials determined by inducible R-factor systems in enteric bacteria
J. Schottel (1974)
Kinetics of chromium (VI) reduction by a type strain Shewanella alga under different growth conditions.
H. Guha (2001)
Implications of microbial heavy metal resistance in the environment
A. Spain (2003)
Low-Temperature Growth of Shewanella oneidensis MR-1
Randa Abboud (2005)
Reduction and phytoavailability of Cr(VI) as influenced by organic manure compost
N. S. Bolan (2003)
A comparative study of various biosorbents for removal of chromium(VI) ions from industrial waste waters
M. Nourbakhsh (1994)
Carcinogenicity and mutagenicity of chromium.
A. Leonard (1980)
Hexavalent chromium reduction by Acinetobacter haemolyticus isolated from heavy-metal contaminated wastewater.
Zainul Akmar Zakaria (2007)
Standard Methods for the examination of water and wastewater
Effect of chromate action on morphology of basalt-inhabiting bacteria
Z. Lin (2006)
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
G. Muyzer (1993)
Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics.
G. Puzon (2005)
Bioprocess Engineering: Basic Concepts
M. Shuler (1991)
High levels of multiple metal resistances and its correlation to antibiotics resistance in environmental isolates
P. K. Dhakephalker (1994)
Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate.
F. A. O. Camargo (2003)
Bergey's Manual of Determinative Bacteriology (5th ed.)
J. H. Brown (1939)
Biosorption of Cr (VI) using a bacterial biofilm supported on granular activated carbon and on zeolite.
Sandra Lameiras (2008)
Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater.
A. Zahoor (2009)
Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent
S. Srivastava (2006)
Aerobic chromate reduction by chromium-resistant bacteria isolated from serpentine soil.
Arundhati Pal (2004)
Isolation and characterization of a Cr(VI)-reduction Ochrobactrum sp. strain CSCr-3 from chromium landfill.
Zhiguo He (2009)
The Role of Bacterial Exopolymers in Metal Sorption and Reduction
A. Dohnalkova (2005)
Bioprocess Engineering: Basic Concepts, 2nd ed
M. L. Shuler (2005)
NAD(P)H-dependent chromium (VI) reductase of Pseudomonas ambigua G-1: a Cr(V) intermediate is formed during the reduction of Cr(VI) to Cr(III).
T. Suzuki (1992)
Reduction of chromate by cell-free extract of Brucella sp. isolated from Cr(VI) contaminated sites.
Urvashi Thacker (2007)
This paper is referenced by
The link between antimicrobial resistance and the content of potentially toxic metals in soil and fertilising Products. Opinion of the Panel on Biological Hazards of the Norwegian Scientific Committee for Food Safety
Yngvild Wasteson (2017)
Microbial action and mechanisms for Cr(VI) removal performance by layered double hydroxide modified zeolite and quartz sand in constructed wetlands.
X. Zhang (2019)
Partial Purification and Characterization of Chromate Reductase of a Novel Ochrobactrum sp. Strain Cr-B4
A. Hora (2015)
Kinetics of bioreduction of hexavalent chromium by poly vinyl alcohol-alginate immobilized cells of Ochrobactrum sp. Cr-B4 and comparison with free cells
A. Hora (2016)
Intracellular uptake and reduction of hexavalent chromium by the cyanobacterium Synechocystis sp. PUPCCC 62
J. S. Khattar (2014)
Removal of chromium from industrial effluents using nanotechnology: a review
Sayak Mitra (2017)
Structure, gene cluster of the O antigen and biological activity of the lipopolysaccharide from the rhizospheric bacterium Ochrobactrum cytisi IPA7.2.
E. Sigida (2019)
Mercuric reductase activity of multiple heavy metal‐resistant Lysinibacillus sphaericus G1
A. Bafana (2015)
Detoxification of hexavalent chromium by Leucobacter sp. uses a reductase with specificity for dihydrolipoamide
A. Sarangi (2016)