Variability In Tannin Content, Chemistry And Activity In A Diverse Group Of Tannin Containing Sorghum Cultivars.
R. C. Kaufman, T. Herald, S. Bean, J. D. Wilson, M. Tuinstra
Published 2013 · Medicine, Chemistry
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BACKGROUND Tannins are large polyphenolic polymers and are known to bind proteins, limiting their digestibility, but are also excellent antioxidants. Numerous studies investigating the functional properties of sorghum tannin have been conducted by comparing grain samples from different sorghum lines without considering the other intrinsic characteristics of the grain. The purpose of this study was to remove the confounding intrinsic factors present in the endosperm so the effect of the tannins could be evaluated utilizing a unique decortication/reconstitution procedure. RESULTS The tannin content of the 14 cultivars tested ranged from 2.3 to 67.2 catechin equivalents. The bran fractions were studied for their impact on protein binding and antioxidant capacity. Protein digestibility by pepsin ranged from 8% to 58% at the highest tannin level addition. Protein binding ranged from 3.11 to 16.33 g blue bovine serum albumin kg⁻¹ bran. Antioxidant capacity ranged from 81.33 to 1122.54 µmol Trolox equivalents g⁻¹ bran. High-performance size-exclusion chromatography detailed molecular size distributions of the tannin polymers and relationship to tannin functionality. CONCLUSION The tannin content and composition play a significant role in determining tannin functionality. These differences will allow for selections of high-tannin sorghums with consideration of the biological activities of the tannins.
This paper references
Determination of protein in tannin-protein precipitates.
A. Hagerman (1980)
Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products.
J. Awika (2003)
Improved characterization of sorghum tannins using size-exclusion chromatography.
Rhett C. Kaufman (2009)
Condensed tannins are only partially responsible for variations in nutrient digestibilities of sorghum grain cultivars
R. Elkin (1996)
Condensed tannins in canola hulls
M. Naczk (1994)
Polyphenol/peptide binding and precipitation.
A. Charlton (2002)
Polyphenolics and tannins effect on in vitro digestibility of selected Acacia species leaves
C. D. Rubanza (2005)
Factors affecting sorghum protein digestibility
K. G. Duodu (2003)
Sorghum Phytochemicals and Their Potential Impact on Human Health.
J. Awika (2004)
Protein precipitating capacity of crude canola tannins: effect of pH, tannin, and protein concentrations
M. Naczk (1996)
Characterisation of tannins and in vitro protein digestibility of several Lotus corniculatus varieties
H. Hedqvist (2000)
Measurement of oxygen radical absorbance capacity in biological samples.
G. Cao (1999)
Interaction of proteins with sorghum tannin: Mechanism, specificity and significance
L. Butler (1984)
Acetonitrile as a buffer additive for free zone capillary electrophoresis separation and characterization of maize (Zeamays L. ) and sorghum (Sorghum bicolor L. Moench) storage proteins.
S. Bean (2000)
Nature of polyphenol-protein interactions
K. Siebert (1996)
Decorticating sorghum to concentrate healthy phytochemicals.
J. Awika (2005)
Studies on the nutritional value of sorghum grains with varying tannin contents for chicks. 1. growth studies
H. S. Rostagno (1973)
Hop (Humulus lupulus L.) proanthocyanidins characterized by mass spectrometry, acid catalysis, and gel permeation chromatography.
Alan W. Taylor (2003)
proanthocyanidins characterized by mass spectrometry , acid catalysis , and gel permeation chromatography
AW Taylor (2003)
High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format.
De-jian Huang (2002)
Multiple interactions between polyphenols and a salivary proline-rich protein repeat result in complexation and precipitation.
N. Baxter (1997)
Protein precipitation methods for quantitation of tannins: a review
H. Makkar (1989)
Evaluation of the Single Kernel Characterization System (SKCS) for Measurement of Sorghum Grain Attributes
Scott R. Bean (2006)
Sorghum food and industrial utilization, in Sorghum: Origin, History, Technology, and Production, ed
LW Rooney (2000)
Use of dye-labeled protein as spectrophotometric assay for protein precipitants such as tannin
T. Asquith (1985)
Three-dimensional structure and dynamics of wine tannin-saliva protein complexes. A multitechnique approach.
C. Simon (2003)
Preferential binding of sorghum tannins with γ-kafirin and the influence of tannin binding on kafirin digestibility and biodegradation
J. Taylor (2007)
HPLC method for the quantification of procyanidins in cocoa and chocolate samples and correlation to total antioxidant capacity.
G. E. Adamson (1999)
Condensed tannins in horse bean seeds: Chemical structure and apparent effects on poultry
J. Martin-Tanguy (1977)
A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain
M. L. Price (1978)
Pepsin digestibility of proteins in sorghum and other major cereals.
E. Mertz (1984)
Relationship between tannin levels, rat growth, and distribution of proteins in sorghum.
R. Jambunathan (1973)
High Molecular Weight Plant Polyphenolics (Tannins) as Biological Antioxidants.
A. Hagerman (1998)
Carbohydrate — Polyphenol Complexation
Y. Cai (1989)
Studies on the Nutritional Value of Sorghum Grains with Varying Tannin Contents for Chicks 2. Amino Acid Digestibility Studies
H. S. Rostagno (1973)
Electron paramagnetic resonance studies of radical species of proanthocyanidins and gallate esters.
W. Bors (2000)
Which polyphenolic compounds contribute to the total antioxidant activities of apple?
R. Tsao (2005)
This paper is referenced by
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K. L. Dunn (2015)
Sorghum (Sorghum bicolor L.): Nutrients, bioactive compounds, and potential impact on human health
Leandro de Morais Cardoso (2017)
Performance of broiler chickens fed South African sorghum-based diets with xylanase
M. Mabelebele (2017)
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Geoffrey P. Morris (2013)
The effects of sorghum fractions on pet food extrusion, digestibility and antioxidant capacity in dogs
Isabella Corsato Alvarenga (2016)
Sorghum Fermentation for Nutritional Improvement
Mohapatra Debabandya (2017)
Effects of Sorghum Polyphenols on In Vitro Starch Digestibility and Protein Profile of Wheat Flour Tortillas
Kristen Lea Dunn (2014)
Cloning of Economically Significant Sorghum Mutant Genes
Sandeep R Marla (2016)
Natural Variation and Genome-Wide Association Study of Antioxidants in a Diverse Sorghum Collection
Davina H. Rhodes (2017)
Effects of sorghum (Sorghum bicolor (L.) Moench) tannins on α-amylase activity and in vitro digestibility of starch in raw and processed flours.
Nyambe Lisulo Mkandawire (2013)
Structural characterization and antioxidant activity of condensed tannins fractionated from sorghum grain
Yiran Jiang (2020)
Effects of Grain Sorghum Muffin on Blood Glucose and Insulin Responses in Prediabetic Men
X. Gu (2014)
Triacylglycerol, fatty acid, and phytochemical profiles in a new red sorghum variety (Ji Liang No. 1) and its antioxidant and anti‐inflammatory properties
Y. Zhang (2019)
Composition, in vitro digestibility, and sensory evaluation of extruded whole grain sorghum breakfast cereals
Nyambe Lisulo Mkandawire (2015)
Genomic Study and Genetic Improvement of Sorghum (Sorghum Bicolor (L) Moench] for High Protein Digestibility
E. Diatta (2018)
Characterization of Phytochemicals and Antioxidant Activities of Specialty Tomatoes
Qi Yuwei (2016)
Assessing the influence of farm fertility amendments, field management, and sorghum genotypes on soil microbial communities and grain quality☆☆☆
Adam B Cobb (2017)
Grain Proteomics of Sorghum: Improving Digestibility, Nutritional Quality and Starch Conversion to Ethanol
J. Cremer (2014)