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Cell‐generated Forces Influence The Viability, Metabolism And Mechanical Properties Of Fibroblast‐seeded Collagen Gel Constructs
C. Berry, J. C. Shelton, D. Lee
Published 2009 · Chemistry, Medicine
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The aim of this study was to investigate the influence of the endogenous forces generated by fibroblast‐mediated contraction, using four individual collagen gel models that differed with respect to the ability of the cells to contract the gel. Human neonatal dermal fibroblasts were seeded in type I collagen and the gels were cast in a racetrack‐shaped mould containing a removable central island. Two of the models were mechanically stressed (20 mm and 10 mm), as complete contraction was prevented by the presence of a central island. The central island was removed in the third model (released) and the final model was cast in a Petri dish and detached, allowing full multi‐axial contraction (SR). Cell viability was maintained in the 10 mm, released and SR models over a 6 day culture period but localized regions of cell death were evident in the 20 mm model. Cell and collagen alignment was developed in the 20 mm and 10 mm models and to a lesser extent in the released model, but was absent in the SR model. Cell proliferation and collagen synthesis was lower in the 20 mm model compared to the other systems and there was evidence of enhanced matrix metalloproteinase production. The mechanical properties of the 20 mm model system were inferior to the 10 mm and released systems. The 10 mm model system induced a high level of cell and matrix orientation and may, therefore, represent the best option for tissue‐engineered ligament repair involving an orientated fibroblast‐seeded collagen gel. Copyright © 2008 John Wiley & Sons, Ltd.
This paper references
Stromelysin-1-deficient fibroblasts display impaired contraction in vitro.
K. Bullard (1999)
Cell - generated forces influence fibroblast - seeded collagen gel constructs 53 release of cell surface fibronectin and downregulation of DNA and protein synthesis
C Oakley (1997)
Cell proliferation and migration on collagen substrata in vitro.
S. Schor (1980)
Fibroblast mechanics in 3D collagen matrices.
S. Rhee (2007)
The fibroblast‐populated collagen matrix as a model of wound healing: a review of the evidence
M. A. Carlson (2004)
Generation of spatially periodic patterns by a mechanical instability: a mechanical alternative to the Turing model.
A. Harris (1984)
Sensitivity of fibroblasts and their cytoskeletons to substratum topographies: topographic guidance and topographic compensation by micromachined grooves of different dimensions.
C. Oakley (1997)
The influence of external uniaxial cyclic strain on oriented fibroblast seeded collagen
CC Berry (2003)
Normal human primary fibroblasts undergo apoptosis in three-dimensional contractile collagen gels.
J. Fluck (1998)
Mechanisms and dynamics of mechanical strengthening in ligament-equivalent fibroblast-populated collagen matrices
D. Huang (2006)
Development of a reconstituted collagen tendon prosthesis. A preliminary implantation study.
J. Goldstein (1989)
Tissue engineering science: Consequences of cell traction force
R. Tranquillo (2004)
Viability of fibroblast‐seeded ligament analogs after autogenous implantation
L. D. Bellincampi (1998)
Three-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells*
T. Haas (1998)
Cell mechanics studied by a reconstituted model tissue.
T. Wakatsuki (2000)
Influence of external uniaxial cyclic strain on oriented fibroblast-seeded collagen gels.
C. Berry (2003)
Association between tension and orientation of periodontal ligament fibroblasts and exogenous collagen fibres in collagen gels in vitro.
C. G. Bellows (1982)
Gelatinase A Activation Is Regulated by the Organization of the Polymerized Actin Cytoskeleton*
J. Tomasek (1997)
Cell growth on collagen: a review of tissue engineering using scaffolds containing extracellular matrix.
F. Silver (1992)
Comparative analysis of different collagen-based biomaterials as scaffolds for long-term culture of human fibroblasts
G. Vaissiere (2006)
Punch-wounded, fibroblast populated collagen matrices: a novel approach for studying cytoskeletal changes in three dimensions by confocal laser scanning microscopy.
W. Baschong (1997)
Effect of gel re‐organization and tensional forces on α2β1 integrin levels in dermal fibroblasts
Gail W. Jenkins (1999)
Fibroblasts in mechancially stressed lattices assume a ‘synthetic’ phenotype
D Kessler (2001)
Stress relaxation of contracted collagen gels: disruption of actin filament bundles, release of cell surface fibronectin, and down-regulation of DNA and protein synthesis.
K. Mochitate (1991)
Fibroblast biology in three-dimensional collagen matrices.
F. Grinnell (2003)
Ligament tissue engineering using synthetic biodegradable fiber scaffolds.
V. Lin (1999)
Duration and orientation of mechanical loads determine fibroblast cyto‐mechanical activation: Monitored by protease release
R. Prajapati (2000)
Biomechanics of knee ligaments: basic concepts and clinical application.
F. Fu (1994)
Biased cell migration of fibroblasts exhibiting contact guidance in oriented collagen gels
R. Dickinson (2006)
Fibroblasts in Mechanically Stressed Collagen Lattices Assume a “Synthetic” Phenotype*
D. Kessler (2001)
Self-organization of tissue-equivalents: the nature and role of contact guidance.
R. Tranquillo (1999)
2000b; Mechanical loading regulates protease production by fibroblasts in threedimensional collagen substrates
RT Prajapati (2000)
Collagen gel overlay induces apoptosis of polarized cells in cultures: disoriented cell death.
M. Tang (1998)
Release of mechanical tension triggers apoptosis of human fibroblasts in a model of regressing granulation tissue.
F. Grinnell (1999)
Unidirectional displacement of cells in fibrillar matrices.
C. Boocock (1989)
A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation
D. Lee (1998)
Role of the cytoskeleton in the reaction of fibroblasts to multiple grooved substrata.
B. Wojciak-Stothard (1995)
Correlation of myosin light chain phosphorylation with isometric contraction of fibroblasts.
M. Kolodney (1993)
Mechanical loading regulates protease production by fibroblasts in three‐dimensional collagen substrates
R. Prajapati (2000)
Fibroblastcollagen-matrix contraction: growth-factor signalling and mechanical loading
F. Grinnell (2000)
Proceedings of the ESSKA Scientific Workshop on Reconstruction of the Anterior and Posterior Cruciate Ligaments
A. Amis (2005)
Localization and activation of type IV collagenase/gelatinase at endothelial focal contacts.
C. Partridge (1997)
Topographical control of cell behaviour: II. Multiple grooved substrata.
P. Clark (1990)
Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix
N. Boudreau (1995)
Suppression of ICE and apoptosis in mammary epithelial cells by ECM
N Boudreau (1995)
Fibroblast contraction occurs on release of tension in attached collagen lattices: Dependency on an organized actin cytoskeleton and serum
J. Tomasek (1992)
Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro.
E. Bell (1979)
Apoptosis, oncosis, and necrosis. An overview of cell death.
G. Majno (1995)
Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by α1β1 and α2β1 integrins
O. Langholz (1995)
Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by alpha 1 beta 1 and alpha 2 beta 1 integrins
O. Langholz (1995)
Contraction of collagen gels seeded with tendon cells.
M.-Y. Chen (2006)
Development of a reconstituted collagen tendon prosthesis.
B. Mckibbin (1990)
Cells transmit spatial information by orienting collagen fibers.
R. Klebe (1989)
Development of Ligament-Like Structural Organization and Properties in Cell-Seeded Collagen Scaffolds in vitro
E. Gentleman (2005)
A new technique to study the mechanical properties of collagen lattices.
J. Chapuis (1992)
Normal human primary fibroblasts undergo apoptosis in 3 D contractile collagen gels
J Fluck (1998)
Disruption of epithelial cell-matrix interactions induces apoptosis
S. Frisch (1994)
Biomechanics of knee ligaments: injury, healing, and repair.
S. Woo (2006)
Regeneration of Achilles tendon with a collagen tendon prosthesis. Results of a one-year implantation study.
Y. P. Kato (1991)
This paper is referenced by
Silica-collagen bionanocomposites as three-dimensional scaffolds for fibroblast immobilization.
M. Desimone (2010)
Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells.
D. Little (2010)
The effect of matrix characteristics on fibroblast proliferation in 3D gels.
K. Bott (2010)
Dynamic quantitative visualization of single cell alignment and migration and matrix remodeling in 3-D collagen hydrogels under mechanical force.
Y. Pang (2011)
Collagen for tendon and ligament repair
J. Buschmann (2017)
Advances in injured tendon engineering with emphasis on the role of collagen implants
A. Oryan (2012)
A hydrogel derived from decellularized dermal extracellular matrix.
M. T. Wolf (2012)
Fibroblast encapsulation in hybrid silica―collagen hydrogels
Martín Federico Desimone (2010)
Rapid generation of collagen-based microtissues to study cell–matrix interactions
Marie-Elena Brett (2016)
Single cell viability measurements in 3D scaffolds using in situ label free imaging by optical coherence microscopy.
J. Dunkers (2012)
Development of fibroblast-seeded collagen gels under planar biaxial mechanical constraints: a biomechanical study
Jin-Jia Hu (2013)
Cell-Generated Forces in Tissue Assembly, Function, and Disease
J. Huynh (2011)
Bio-inspired silica-collagen materials: applications and perspectives in the medical field.
S. Heinemann (2013)
In vitro studies and preliminary in vivo evaluation of silicified concentrated collagen hydrogels.
Martín F. Desimone (2011)
Working together: the combined application of a magnetic field and penetratin for the delivery of magnetic nanoparticles to cells in 3D.
H. Child (2011)