Chondrogenic Potential Of Injectable κ-carrageenan Hydrogel With Encapsulated Adipose Stem Cells For Cartilage Tissue-engineering Applications.
Elena Geta Popa, Sofia G. Caridade, João F Mano, Rui L. Reis, Manuela E Gomes
Published 2015 · Medicine, Biology
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Due to the limited self-repair capacity of cartilage, regenerative medicine therapies for the treatment of cartilage defects must use a significant amount of cells, preferably applied using a hydrogel system that can promise their delivery and functionality at the specific site. This paper discusses the potential use of κ-carrageenan hydrogels for the delivery of stem cells obtained from adipose tissue in the treatment of cartilage tissue defects. The developed hydrogels were produced by an ionotropic gelation method and human adipose stem cells (hASCs) were encapsulated in 1.5% w/v κ-carrageenan solution at a cell density of 5 × 10(6) cells/ml. The results from the analysis of the cell-encapsulating hydrogels, cultured for up to 21 days, indicated that κ-carrageenan hydrogels support the viability, proliferation and chondrogenic differentiation of hASCs. Additionally, the mechanical analysis demonstrated an increase in stiffness and viscoelastic properties of κ-carrageenan gels with their encapsulated cells with increasing time in culture with chondrogenic medium. These results allowed the conclusion that κ-carrageenan exhibits properties that enable the in vitro functionality of encapsulated hASCs and thus may provide the basis for new successful approaches for the treatment of cartilage defects.
This paper references
Preparation and in vitro evaluation of verapamil HCl and ibuprofen containing carrageenan beads.
Oya Sipahigil (2001)
Toward a new generation of therapeutics
Satya Prakash (2006)
Thermoreversible hydrogel scaffolds for articular cartilage engineering.
John P. Fisher (2004)
Kappa-carrageenan interactions in systems containing casein micelles and polysaccharide stabilizers
Paul A Spagnuolo (2005)
Current strategies for articular cartilage repair.
Samantha N Redman (2005)
A simple, rapid, and sensitive DNA assay procedure.
Cesar Labarca (1980)
Current strategies for cell delivery in cartilage and bone regeneration.
Michael Sittinger (2004)
A novel method for the isolation of subpopulations of rat adipose stem cells with different proliferation and osteogenic differentiation potentials.
Tommaso Rada (2011)
Adipose-derived stem cells for regenerative medicine.
Jeffrey M. Gimble (2007)
Alginate/lactose-modified chitosan hydrogels: a bioactive biomaterial for chondrocyte encapsulation.
Eleonora Marsich (2008)
The role of the extracellular matrix in skeletal muscle development.
Sandra G. Velleman (1999)
Chondrogenic differentiation of mesenchymal stem cells induced by collagen-based hydrogel: an in vivo study.
Li Zheng (2009)
Adipose-tissue engineering: taking advantage of the properties of human adipose-derived stem/stromal cells.
Monique Vallée (2009)
κ -Carrageenan—Protein Interactions: Effect of Proteins on Polysaccharide Gelling and Textural Properties
R. I. Baeza (2002)
Distinct Stem Cells Subpopulations Isolated from Human Adipose Tissue Exhibit Different Chondrogenic and Osteogenic Differentiation Potential
Tommaso Rada (2010)
Current state of cartilage tissue engineering
Richard Tuli (2003)
Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels.
Nathaniel S Hwang (2006)
Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.
Geoffrey R. Erickson (2002)
Human adipose tissue is a source of multipotent stem cells.
Patricia A. Zuk (2002)
Effect of potassium chloride and cationic drug on swelling, erosion and release from k-carrageenanmatrices.AAPS PharmSciTech
S Naim (2004)
Cytocompatibility and response of osteoblastic-like cells to starch-based polymers: effect of several additives and processing conditions.
Manuela E Gomes (2001)
Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
Pedro P. Carvalho (2011)
Adipose-derived stem cells: characterization and current application in orthopaedic tissue repair.
Hazel Tapp (2009)
2010 Nicolas Andry Award: Multipotent Adult Stem Cells from Adipose Tissue for Musculoskeletal Tissue Engineering
Farshid Guilak (2010)
Dynamic Mechanical Analysis in Polymers for Medical Applications
João F. Mano (2002)
A comparison of healthy human and swine articular cartilage dynamic indentation mechanics
Sarah Ronken (2012)
Applied Statistics for Engineers and Physical Scientists, Third Edition by Johannes Ledolter, Robert V. Hogg
Erkki P. Liski (2009)
Applied Statistics for Engineers and Physical Scientists
Eric R. Ziegel (1991)
Cartilage tissue engineering for degenerative joint disease.
Dobrila Nesic (2006)
Study of the influence of beta-radiation on the properties and mineralization of different starch-based biomaterials.
Ana L. Oliveira (2005)
Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures.
John D. Kisiday (2008)
Static and dynamic mechanical properties of extracellular matrix synthesized by cultured chondrocytes
Shogo Miyata (2004)
Structure of aggregating kappa-carrageenan fractions studied by light scattering.
Véronique Meunier (2001)
Composition and dynamics of articular cartilage: structure, function, and maintaining healthy state.
Nathaniel P. Cohen (1998)
Stability of hydrogels used in cell encapsulation: An in vitro comparison of alginate and agarose.
Molly S. Shoichet (1996)
Analysis of relative gene expression data using rea l—time quantitative PCR a nd the 2 一 ct method
J. Brand Kenneth (2001)
The Carrageenan Connection IV
Harris J. Bixler (1994)
The Effect of Storage Time on Adipose-Derived Stem Cell Recovery from Human Lipoaspirates
Pedro P. Carvalho (2011)
Transcriptional mechanisms of chondrocyte differentiation.
Benoit de Crombrugghe (2000)
Dynamic mechanical analysis in polymers for medi - cal applications
JF Mano (2002)
Novel starch - based scaffolds for
AJ Salgado (2004)
The control of chondrogenesis.
Mary B Goldring (2006)
Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis.
Darko Bosnakovski (2006)
Cation effects on sol–gel and gel–sol phase transitions of κ-carrageenan–water system
Selim Kara (2003)
Hydrogels in regenerative medicine.
Brandon V. Slaughter (2009)
The chondrocyte: biology and clinical application.
Zhen Lin (2006)
Novel starch-based scaffolds for bone tissue engineering: cytotoxicity, cell culture, and protein expression.
António J Salgado (2004)
In situ chondrogenic differentiation of human adipose tissue-derived stem cells in a TGF-beta1 loaded fibrin-poly(lactide-caprolactone) nanoparticulate complex.
Youngmee Jung (2009)
Effect of potassium chloride and cationic drug on swelling, erosion and release from κ-carrageenan matrices
Syed Rashid Naim (2008)
Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair
John D. Kisiday (2002)
Agarose Gel as Biomaterial or Scaffold for Implantation Surgery: Characterization, Histological and Histomorphometric Study on Soft Tissue Response
Elena Maria Varoni (2012)
Photo-crosslinkable hyaluronan as a scaffold
DL Nettles (2004)
2011b; Use of animal protein-free products for passaging adherent human adipose-derived stromal/ stem cells
PP Carvalho (2011)
K+ and Na+ effects on the gelation properties of k-carrageenan
Marco Mangione (2005)
Soluble Signalling Factors Derived from Differentiated Cartilage Tissue Affect Chondrogenic Differentiation of Rat Adult Marrow Stromal Cells
Nazish Ahmed (2007)
Repair of Chronic Osteochondral Defects Using Predifferentiated Mesenchymal Stem Cells in an Ovine Model
Matthias Zscharnack (2010)
A novel injectable scaffold for cartilage tissue engineering using adipose-derived adult stem cells.
Yiyong Wei (2008)
: cytotoxicity , cell culture , and protein expression
MS Shoichet (1996)
A comparison between the chondrogenic potential of human bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) taken from the same donors.
Hassan Afizah (2007)
Solute diffusivity correlates with mechanical properties and matrix density of compressed articular cartilage.
Robin C. Evans (2005)
Review: Hydrogels for cell immobilization.
A C Jen (1996)
Alginate hydrogels as biomaterials.
Alexander Augst (2006)
Dynamic elastic modulus of porcine articular cartilage determined at two different levels of tissue organization by indentation-type atomic force microscopy.
Martin Stolz (2004)
Hydrogels for tissue engineering: scaffold design variables and applications.
Jeanie L Drury (2003)
Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds.
Hani A Awad (2004)
Hypertrophy in Mesenchymal Stem Cell Chondrogenesis: Effect of TGF-β Isoforms and Chondrogenic Conditioning
Michael B. Mueller (2010)
Differences between sub-populations of cultured bovine articular chondrocytes. I. Morphology and cartilage matrix production.
Margaret B. Aydelotte (1988)
P. Lennart (2006)
This paper is referenced by
Marine Origin Polysaccharides in Drug Delivery Systems
Matias J. Cardoso (2016)
Fabrication of endothelial cell-laden carrageenan microfibers for microvascularized bone tissue engineering applications.
Silvia M. Mihaila (2014)
Whey protein isolate for the preparation of covalent immobilization beads
Marwa I Wahba (2018)
Marine Seaweed Polysaccharides-Based Engineered Cues for the Modern Biomedical Sector
Muhammad Bilal (2019)
Biomaterial-based scaffolds – current status and future directions
Tarun Garg (2014)
Carrageenan based hydrogels for drug delivery, tissue engineering and wound healing.
Ramanathan Yegappan (2018)
Cartilage tissue engineering combining microspheroid building blocks and microneedle arrays
Shawn Patrick Grogan (2019)
Silk fibroin-based injectable hydrogels for brain tissue engineering applications
Wei Sun (2014)
Engineering articular cartilage using newly developed carrageenan basedhydrogels
Elena Geta Popa (2014)
Bio-inspired Aloe vera sponges for biomedical applications.
S. Silva (2014)
Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications
Thomas H. Jovic (2019)
Development and characterization of carbohydrate-based thermosensitive hydrogels for cartilage tissue engineering
Cecília Buzatto Westin (2020)
Recent Advances in the Use of Algal Polysaccharides for Skin Wound Healing.
Suneel Kumar (2019)
Thermal-Recoverable Tough Hydrogels Enhanced by Porphyrin Decorated Graphene Oxide
Jilong Wang (2019)
Sulfated polysaccharide-based scaffolds for orthopaedic tissue engineering.
Jeremy Nicolas Dinoro (2019)
Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
Yi Deng (2018)
Strategies to Maximize the Potential of Marine Biomaterials as a Platform for Cell Therapy
Hyeongmin Kim (2016)
An injectable mechanically robust hydrogel of Kappa-carrageenan-dopamine functionalized graphene oxide for promoting cell growth.
Hamidreza Mokhtari (2019)
Fabrication of micropatterned alginate-gelatin and k-carrageenan hydrogels of defined shapes using simple wax mould method as a platform for stem cell/induced Pluripotent Stem Cells (iPSC) culture.
Shanmugam Bhaskar Vignesh (2018)
Injectable hydrogels: a new paradigm for osteochondral tissue engineering.
Yogendra Pratap Singh (2018)
Studies on equilibrium moisture absorption of kappa carrageenan.
Shyam Bajpai (2013)
Seaweed polysaccharide-based hydrogels used for the regeneration of articular cartilage
Elena Geta Popa (2015)
Current approaches and future perspectives on strategies for the development of personalized tissue engineering therapies
Lisete S. Neves (2016)
The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review
Andy Goldberg (2017)
Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting.
Myoung Hwan Kim (2019)
Glycosaminoglycan-based biomaterials for growth factor and cytokine delivery: Making the right choices
Daniel Hachim (2019)
Injectable Hyaluronic Acid Hydrogels Enriched with Platelet Lysate as a Cryostable Off-the-Shelf System for Cell-Based Therapies
Lisete S. Neves (2017)
Cell Encapsulation Systems Toward Modular Tissue Regeneration: From Immunoisolation to Multifunctional Devices
Clara R Correia (2020)
Advances of Stem Cell-Laden Hydrogels With Biomimetic Microenvironment for Osteochondral Repair
Bingbing Xu (2020)
Anisotropic Shape-Memory Alginate Scaffolds Functionalized with Either Type I or Type II Collagen for Cartilage Tissue Engineering.
Henrique V. Almeida (2017)
Preparation and characterization of κ-carrageenan hydrogel for controlled release of copper and manganese micronutrients
Gulen Oytun Akalin (2019)
Magnetically-Responsive Hydrogels for Modulation of Chondrogenic Commitment of Human Adipose-Derived Stem Cells
Elena Geta Popa (2016)See more