Chondrogenic Potential Of Injectable κ-carrageenan Hydrogel With Encapsulated Adipose Stem Cells For Cartilage Tissue-engineering Applications.
E. Popa, S. Caridade, J. Mano, R. Reis, M. Gomes
Published 2015 · Medicine, Biology
Download PDFAnalyze on Scholarcy
Due to the limited self-repair capacity of cartilage, regenerative medicine therapies for the treatment of cartilage defects must use a significant amount of cells, preferably applied using a hydrogel system that can promise their delivery and functionality at the specific site. This paper discusses the potential use of κ-carrageenan hydrogels for the delivery of stem cells obtained from adipose tissue in the treatment of cartilage tissue defects. The developed hydrogels were produced by an ionotropic gelation method and human adipose stem cells (hASCs) were encapsulated in 1.5% w/v κ-carrageenan solution at a cell density of 5 × 10(6) cells/ml. The results from the analysis of the cell-encapsulating hydrogels, cultured for up to 21 days, indicated that κ-carrageenan hydrogels support the viability, proliferation and chondrogenic differentiation of hASCs. Additionally, the mechanical analysis demonstrated an increase in stiffness and viscoelastic properties of κ-carrageenan gels with their encapsulated cells with increasing time in culture with chondrogenic medium. These results allowed the conclusion that κ-carrageenan exhibits properties that enable the in vitro functionality of encapsulated hASCs and thus may provide the basis for new successful approaches for the treatment of cartilage defects.
This paper references
Preparation and in vitro evaluation of verapamil HCl and ibuprofen containing carrageenan beads.
O. Sipahigil (2001)
Toward a new generation of therapeutics
S. Prakash (2006)
Current strategies for cell delivery in cartilage and bone regeneration.
M. Sittinger (2004)
Thermoreversible hydrogel scaffolds for articular cartilage engineering.
J. Fisher (2004)
Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.
G. Erickson (2002)
Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels.
N. Hwang (2006)
Chondrogenic differentiation of mesenchymal stem cells induced by collagen-based hydrogel: an in vivo study.
L. Zheng (2010)
Adipose-tissue engineering: taking advantage of the properties of human adipose-derived stem/stromal cells.
M. Vallée (2009)
Alginate/lactose-modified chitosan hydrogels: a bioactive biomaterial for chondrocyte encapsulation.
E. Marsich (2008)
The role of the extracellular matrix in skeletal muscle development.
S. Velleman (1999)
Hydrophilic excipients modulate the time lag of time-controlled disintegrating press-coated tablets
S. Lin (2008)
Kappa-carrageenan interactions in systems containing casein micelles and polysaccharide stabilizers
P. A. Spagnuolo (2005)
A simple, rapid, and sensitive DNA assay procedure.
C. Labarca (1980)
Current strategies for articular cartilage repair.
S. Redman (2005)
Adipose-derived stem cells for regenerative medicine.
J. Gimble (2007)
A novel method for the isolation of subpopulations of rat adipose stem cells with different proliferation and osteogenic differentiation potentials.
T. Rada (2011)
Study of the influence of beta-radiation on the properties and mineralization of different starch-based biomaterials.
A. Oliveira (2005)
Cartilage tissue engineering for degenerative joint disease.
D. Nesic (2006)
Applied Statistics for Engineers and Physical Scientists
E. Ziegel (1991)
Adipose-derived stem cells: characterization and current application in orthopaedic tissue repair.
H. Tapp (2009)
2010 Nicolas Andry Award: Multipotent Adult Stem Cells from Adipose Tissue for Musculoskeletal Tissue Engineering
F. Guilak (2010)
Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
P. P. Carvalho (2011)
Transcriptional mechanisms of chondrocyte differentiation.
B. de Crombrugghe (2000)
Dynamic mechanical analysis in polymers for medi - cal applications
JF Mano (2002)
Novel starch - based scaffolds for
AJ Salgado (2004)
The Effect of Storage Time on Adipose-Derived Stem Cell Recovery from Human Lipoaspirates
P. P. Carvalho (2011)
Stability of hydrogels used in cell encapsulation: An in vitro comparison of alginate and agarose
M. S. Shoichet (1996)
Composition and dynamics of articular cartilage: structure, function, and maintaining healthy state.
N. Cohen (1998)
The Carrageenan Connection IV
H. Bixler (1994)
Analysis of relative gene expression data using rea l—time quantitative PCR a nd the 2 一 ct method
J. Kenneth (2001)
Distinct Stem Cells Subpopulations Isolated from Human Adipose Tissue Exhibit Different Chondrogenic and Osteogenic Differentiation Potential
T. Rada (2010)
Current state of cartilage tissue engineering
R. Tuli (2003)
κ -Carrageenan—Protein Interactions: Effect of Proteins on Polysaccharide Gelling and Textural Properties
R. Baeza (2002)
Novel starch-based scaffolds for bone tissue engineering: cytotoxicity, cell culture, and protein expression.
A. Salgado (2004)
The chondrocyte: biology and clinical application.
Z. Lin (2006)
Effect of potassium chloride and cationic drug on swelling, erosion and release from κ-carrageenan matrices
S. Naim (2008)
In situ chondrogenic differentiation of human adipose tissue-derived stem cells in a TGF-beta1 loaded fibrin-poly(lactide-caprolactone) nanoparticulate complex.
Youngmee Jung (2009)
Cytocompatibility and response of osteoblastic-like cells to starch-based polymers: effect of several additives and processing conditions.
M. Gomes (2001)
Human adipose tissue is a source of multipotent stem cells.
P. Zuk (2002)
Effect of potassium chloride and cationic drug on swelling, erosion and release from k-carrageenanmatrices.AAPS PharmSciTech
S Naim (2004)
Agarose Gel as Biomaterial or Scaffold for Implantation Surgery: Characterization, Histological and Histomorphometric Study on Soft Tissue Response
E. Varoni (2012)
Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair
J. Kisiday (2002)
2011b; Use of animal protein-free products for passaging adherent human adipose-derived stromal/ stem cells
PP Carvalho (2011)
Photo-crosslinkable hyaluronan as a scaffold
DL Nettles (2004)
Static and dynamic mechanical properties of extracellular matrix synthesized by cultured chondrocytes
Shogo Miyata (2004)
Evaluation of adult equine bone marrow‐ and adipose‐derived progenitor cell chondrogenesis in hydrogel cultures
J. Kisiday (2008)
Structure of aggregating kappa-carrageenan fractions studied by light scattering.
Véronique Meunier (2001)
Hydrogels for tissue engineering: scaffold design variables and applications.
Jeanie L Drury (2003)
Differences between sub-populations of cultured bovine articular chondrocytes. I. Morphology and cartilage matrix production.
M. Aydelotte (1988)
Hypertrophy in Mesenchymal Stem Cell Chondrogenesis: Effect of TGF-β Isoforms and Chondrogenic Conditioning
M. Mueller (2010)
P. Lennart (2006)
Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds.
H. Awad (2004)
Hydrogels in regenerative medicine.
B. V. Slaughter (2009)
Cation effects on sol–gel and gel–sol phase transitions of κ-carrageenan–water system
Selim Kara (2003)
Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis
D. Bosnakovski (2006)
The control of chondrogenesis
M. Goldring (2006)
Dynamic elastic modulus of porcine articular cartilage determined at two different levels of tissue organization by indentation-type atomic force microscopy.
M. Stolz (2004)
Alginate hydrogels as biomaterials.
Alexander Augst (2006)
A comparison between the chondrogenic potential of human bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) taken from the same donors.
Hassan Afizah (2007)
Solute diffusivity correlates with mechanical properties and matrix density of compressed articular cartilage.
R. C. Evans (2005)
Review: Hydrogels for cell immobilization
A. Jen (1996)
Repair of Chronic Osteochondral Defects Using Predifferentiated Mesenchymal Stem Cells in an Ovine Model
M. Zscharnack (2010)
K+ and Na+ effects on the gelation properties of k-carrageenan
Marco Mangione (2005)
Soluble Signalling Factors Derived from Differentiated Cartilage Tissue Affect Chondrogenic Differentiation of Rat Adult Marrow Stromal Cells
N. Ahmed (2007)
: cytotoxicity , cell culture , and protein expression
MS Shoichet (1996)
A novel injectable scaffold for cartilage tissue engineering using adipose‐derived adult stem cells
Y. Wei (2008)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
Applied Statistics for Engineers and Physical Scientists, Third Edition by Johannes Ledolter, Robert V. Hogg
Erkki P. Liski (2009)
Dynamic Mechanical Analysis in Polymers for Medical Applications
J. F. Mano (2002)
Photocrosslinkable Hyaluronan as a Scaffold for Articular Cartilage Repair
D. L. Nettles (2004)
A comparison of healthy human and swine articular cartilage dynamic indentation mechanics
S. Ronken (2012)
This paper is referenced by
Marine Origin Polysaccharides in Drug Delivery Systems
M. J. Cardoso (2016)
Fabrication of endothelial cell-laden carrageenan microfibers for microvascularized bone tissue engineering applications.
Silvia M. Mihaila (2014)
Engineering articular cartilage using newly developed carrageenan basedhydrogels
E. Popa (2014)
Formation of porous HPCL/LPCL/HA scaffolds with supercritical CO2 gas foaming method.
M. Zahedi Moghadam (2017)
Carrageenan based hydrogels for drug delivery, tissue engineering and wound healing.
Ramanathan Yegappan (2018)
Biomaterial-based scaffolds – current status and future directions
Tarun Garg (2014)
Hydrogels for controlled pulmonary delivery.
Ju Du (2013)
Sulfated polysaccharide-based scaffolds for orthopaedic tissue engineering.
J. Dinoro (2019)
Dual Physically Cross-Linked κ-Carrageenan-Based Double Network Hydrogels with Superior Self-Healing Performance for Biomedical Application.
Yi Deng (2018)
Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications
T. Jovic (2019)
Development and characterization of carbohydrate-based thermosensitive hydrogels for cartilage tissue engineering
Cecília Buzatto Westin (2020)
Preventing Obstructions of Nanosized Drug Delivery Systems by the Extracellular Matrix
Luise Tomasetti (2018)
Bio-inspired Aloe vera sponges for biomedical applications.
S. Silva (2014)
Current approaches and future perspectives on strategies for the development of personalized tissue engineering therapies
L. S. Neves (2016)
Seaweed polysaccharide-based hydrogels used for the regeneration of articular cartilage
E. Popa (2015)
Injectable Hydrogels for Biomedical Applications
Biji Balakrishnan (2016)
Toward the development of biomimetic injectable and macroporous biohydrogels for regenerative medicine.
K. Flégeau (2017)
Anisotropic Shape-Memory Alginate Scaffolds Functionalized with Either Type I or Type II Collagen for Cartilage Tissue Engineering.
H. V. Almeida (2017)
Magnetically-Responsive Hydrogels for Modulation of Chondrogenic Commitment of Human Adipose-Derived Stem Cells
Elena Geta Popa (2016)
Preparation and characterization of κ-carrageenan hydrogel for controlled release of copper and manganese micronutrients
Gulen Oytun Akalin (2019)
Fabrication of micropatterned alginate-gelatin and k-carrageenan hydrogels of defined shapes using simple wax mould method as a platform for stem cell/induced Pluripotent Stem Cells (iPSC) culture.
Shanmugam Bhaskar Vignesh (2018)
Studies on equilibrium moisture absorption of kappa carrageenan.
Shyam Bajpai (2013)
Injectable hydrogels: a new paradigm for osteochondral tissue engineering.
Yogendra Pratap Singh (2018)
2.11 Polymers of Biological Origin
Simone S Silva (2017)
Introduction to Seaweed Polysaccharides
Jayachandran Venkatesan (2017)
Whey protein isolate for the preparation of covalent immobilization beads
M. Wahba (2018)
Silk fibroin-based injectable hydrogels for brain tissue engineering applications
Wei Sun (2014)
Biomaterials in Preclinical Approaches for Engineering Skeletal Tissues
Márcia T. Rodrigues (2015)
Applications of Carrageenan in Advanced Drug Delivery
Jian Guan (2017)
Temperature-responsive bioactive hydrogels based on a multifunctional recombinant elastin-like polymer
Vítor E Santo (2015)
Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting.
Myoung Hwan Kim (2019)
The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review
A. Goldberg (2017)See more