Online citations, reference lists, and bibliographies.
← Back to Search

Fed‐batch Methanol Feeding Strategy For Recombinant Protein Production By Pichia Pastoris In The Presence Of Co‐substrate Sorbitol

E. Çelik, P. Çalık, S. Oliver
Published 2009 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Batch‐wise sorbitol addition as a co‐substrate at the induction phase of methanol fed‐batch fermentation by Pichia pastoris (Mut+) was proposed as a beneficial recombinant protein production strategy and the metabolic responses to methanol feeding rate in the presence of sorbitol was systematically investigated. Adding sorbitol batch‐wise to the medium provided the following advantages over growth on methanol alone: (a) eliminating the long lag‐phase for the cells and reaching ‘high cell density production’ at t = 24 h of the process (CX = 70 g CDW/l); (b) achieving 1.8‐fold higher recombinant human erythropoietin (rHuEPO) (at t = 18 h); (c) reducing specific protease production 1.2‐fold; (d) eliminating the lactic acid build‐up period; (e) lowering the oxygen uptake rate two‐fold; and (f) obtaining 1.4‐fold higher overall yield coefficients. The maximum specific alcohol oxidase activity was not affected in the presence of sorbitol, and it was observed that sorbitol and methanol were utilized simultaneously. Thus, in the presence of sorbitol, 130 mg/l rHuEPO was produced at t = 24 h, compared to 80 mg/l rHuEPO (t = 24 h) on methanol alone. This work demonstrates not only the ease and efficiency of incorporating sorbitol to fermentations by Mut+ strains of P. pastoris for the production of any bio‐product, but also provides new insights into the metabolism of the methylotrophic yeast P. pastoris. Copyright © 2009 John Wiley & Sons, Ltd.
This paper references
10.1002/BIT.22566
Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systems.
B. Bandyopadhyay (1967)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. K. Laemmli (1970)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1002/ELPS.1150080203
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
H. Blum (1987)
10.1093/NAR/15.9.3859
Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris.
J. F. Tschopp (1987)
10.1111/j.1749-6632.1990.tb24257.x
Fermentation Development of Recombinant Pichia pastoris Expressing the Heterologous Gene: Bovine Lysozyme
R. A. Brierley (1990)
10.1111/J.1574-6968.1990.TB04925.X
Emerging applications of the methylotrophic yeasts.
G. H. Wegner (1990)
10.1016/S0378-1119(96)00672-5
Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris.
K. Sreekrishna (1997)
10.1016/S0141-0229(97)00042-2
Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fedbatch fermentations
V. Chiruvolu (1997)
10.1385/0-89603-421-6:107
High cell-density fermentation.
J. Stratton (1998)
10.1016/S0141-0229(98)00069-6
Oxygen transfer effects in serine alkaline protease fermentation by Bacillus licheniformis: use of citric acid as the carbon source
P. Çalık (1998)
10.1007/BF02942184
Fermentation strategies for recombinant protein expression in the methylotrophic yeastPichia pastoris
Wenhui Zhang (2000)
10.1002/1097-0290(20000805)69:3<301::AID-BIT8>3.0.CO;2-4
Oxygen-transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis.
P. Çalık (2000)
10.1002/BIT.1125
Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations.
S. Hellwig (2001)
10.1016/S1389-1723(01)80321-2
Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris.
M. Inan (2001)
10.1002/BIT.10297
Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris.
Feng F Hong (2002)
10.1016/J.MOLCATB.2003.09.001
Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase
Ana M O Azevedo (2004)
10.1002/BIT.20051
Methanol induction optimization for scFv antibody fragment production in Pichia pastoris
A. Cunha (2004)
10.1016/J.FEMSYR.2004.12.011
The methylotrophic yeast Pichia pastoris as a host for the expression and production of thermostable xylanase from the bacterium Rhodothermus marinus.
S. Ramchuran (2005)
10.1016/J.BEJ.2005.04.005
Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures
O. Cós (2005)
10.1002/BIT.20318
Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: case study with recombinant ovine interferon-tau.
J. Sinha (2005)
10.1016/J.PROCBIO.2004.07.022
Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production
Wei Zhang (2005)
10.1016/J.ENZMICTEC.2004.06.010
Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production
J. Xie (2005)
10.1016/J.BEJ.2006.04.004
Increased total air pressure versus oxygen limitation for enhanced oxygen transfer and product formation in a Pichia pastoris recombinant protein process
Theppanya Charoenrat (2006)
10.1021/bp050373+
Effects of pH Strategy on endo‐ and exo‐Metabolome Profiles and Sodium Potassium Hydrogen Ports of β‐Lactamase‐Producing Bacilluslicheniformis
Nazar Ileri (2006)
10.1016/J.ENZMICTEC.2006.01.027
Quantitative characterization of the regulation of the synthesis of alcohol oxidase and of the expression of recombinant avidin in a Pichia pastoris Mut+ strain
C. Jungo (2006)
10.1002/BIT.21484
Twenty‐four‐well plate miniature bioreactor high‐throughput system: Assessment for microbial cultivations
Kevin Isett (2007)
10.1016/J.JBIOTEC.2007.02.025
Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris.
R. Ramón (2007)
10.1016/J.JBIOTEC.2007.05.019
A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris.
C. Jungo (2007)
10.1111/j.1365-2672.2007.03448.x
Production of recombinant human erythropoietin from Pichia pastoris and its structural analysis
E. Çelik (2007)
10.1016/j.jbiotec.2008.04.009
Effects of different glycerol feeding strategies on S-adenosyl-l-methionine biosynthesis by P(GAP)-driven Pichia pastoris overexpressing methionine adenosyltransferase.
Xiao-qing Hu (2008)
10.1002/BIT.21565
Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut+ strain
J. Schenk (2008)
10.1021/IE071613O
Use of Biodiesel Byproduct Crude Glycerol as the Carbon Source for Fermentation Processes by Recombinant Pichia pastoris
E. Çelik (2008)
Influence of specific growth rate on specific productivity and glycosylation of a Copyright 
J Schenk (2008)
Influence of specific growth rate on specific productivity and glycosylation of a Copyright  2009
J Schenk (2008)
Oliver recombinant avidin produced by a Pichia pastoris Mut + strain
E Çelik



This paper is referenced by
10.3389/fonc.2021.585457
Fermentation, Purification, and Tumor Inhibition of a Disulfide-Stabilized Diabody Against Fibroblast Growth Factor-2
Simin Zhang (2021)
10.1016/j.bej.2021.108262
Enhancing HSA-GCSFm fusion protein production by Pichia pastoris with an on-line model-based exponential and DO-stat control modes
Luqiang Jia (2021)
10.1101/2021.09.12.459941
PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol uptake regardless of the secondary carbon source: a simplifying principle for predicting recombinant protein production
Anamika Singh (2021)
10.3390/PR9050876
Optimization of a Recombinant Lectin Production in Pichia pastoris Using Crude Glycerol in a Fed-Batch System
D. M. Palmerín-Carreño (2021)
10.1038/s41598-020-78711-6
Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
Claudia R. Gabriela Matías Luis Enrique S. Patricio O. Lea Arbeitman Auge Blaustein Bredeston Corapi Craig Co (2020)
10.1101/2020.09.17.300335
Structural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in Pichia pastoris and Mammalian Cells
C. R. Arbeitman (2020)
10.1016/j.bej.2020.107782
Ethanol fed-batch bioreactor operation to enhance therapeutic protein production in Pichia pastoris under hybrid-architectured ADH2 promoter
Omar Wehbe (2020)
10.3390/biom10030434
Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in Pichia pastoris for Solid-State NMR
R. Munro (2020)
10.1007/s00449-020-02427-0
Development of a dual specific growth rate-based fed-batch process for production of recombinant human granulocyte colony-stimulating factor in Pichia pastoris
A. Gautam (2020)
10.1007/s00449-020-02452-z
Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant Bacillus megaterium in fed-batch bioreactors
Murat Akdoğan (2020)
10.1016/j.enzmictec.2020.109552
High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris.
Z. E. Duman (2020)
10.1007/s00253-020-10796-5
Hybrid-architectured double-promoter expression systems enhance and upregulate-deregulated gene expressions in Pichia pastoris in methanol-free media
Irem Demir (2020)
10.1002/jcp.29583
Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins
M. Karbalaei (2020)
10.1002/bab.1785
Accurate and cost‐effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pastoris by using neural network based soft sensor
S. Hosseini (2019)
Development of new cultivation strategies to enhance heterologous protein production in Pichia pastoris
B. Wang (2019)
10.1016/j.jbiotec.2018.12.002
Comparison and implementation of different control strategies for improving production of rHSA using Pichia pastoris.
Priyanka (2019)
10.3844/ofsp.12797
The Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in Pichia pastoris KM71
Asri Kurniatin (2019)
10.1007/s00705-019-04332-8
Impact of hepatitis B virus genotype F on in vitro diagnosis: detection efficiency of HBsAg from Amerindian subgenotypes F1b and F4
María J. Limeres (2019)
10.1080/07388551.2018.1554620
Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production
Wan-Cang Liu (2019)
10.1016/J.ENZMICTEC.2019.109366
Sorbitol/methanol mixed induction reduces process impurities and improves centrifugal dewatering in Pichia pastoris culture.
Baolong Wang (2019)
10.3390/biom9100568
Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
N. Maity (2019)
10.1002/jctb.6192
Comparative study of μ ‐stat methanol feeding control in fed‐batch fermentation of Pichia pastoris producing HBsAg: an open‐loop control versus recurrent artificial neural network‐based feedback control
A. Beiroti (2019)
10.4103/1735-5362.228953
Bioprocess and downstream optimization of recombinant human growth hormone in Pichia pastoris
Saeed Azadi (2018)
10.1007/s11033-018-4244-2
Metabolic engineering of Pichia pastoris GS115 for enhanced pentose phosphate pathway (PPP) flux toward recombinant human interferon gamma (hIFN-γ) production
Ashish A. Prabhu (2018)
10.1186/s40643-018-0211-y
Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris
Olufemi Emmanuel Bankefa (2018)
10.1002/JCTB.5696
Hybrid fed-batch bioreactor operation design: control of substrate uptake enhances recombinant protein production in high-cell-density fermentations
P. Çalık (2018)
10.1016/j.mimet.2018.04.013
New insight into the method of posttransformational vector amplification (PTVA) in Pichia pastoris.
Liangcheng Jiao (2018)
10.1016/j.compchemeng.2018.07.013
Model-based optimization of biopharmaceutical manufacturing in Pichia pastoris based on dynamic flux balance analysis
Victor N. Emenike (2018)
10.1007/s10295-017-1988-y
Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pastoris
Jinjia Wang (2017)
10.1016/j.ijbiomac.2016.09.102
Bioprocess for the production of recombinant HAP phytase of the thermophilic mold Sporotrichum thermophile and its structural and biochemical characteristics.
Anay Kumar Maurya (2017)
10.3389/fmicb.2017.00493
Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications
D. Parashar (2017)
10.22037/IJPR.2017.2130
Evaluation of Sorbitol-Methanol Co-Feeding Strategy on Production of Recombinant Human Growth Hormone in Pichia Pastoris
Saeed Azadi (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar