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Quantitating Staphylococcal Enterotoxin B In Diverse Media Using A Portable Fiber-optic Biosensor.

L. Tempelman, K. D. King, G. P. Anderson, F. Ligler
Published 1996 · Chemistry, Medicine

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A new, portable fiber-optic biosensor has been used to detect staphylococcal enterotoxin B, a causative agent of food poisoning, at levels as low as 0.5 ng/ml in buffer. The toxin (SEB) can also be detected and quantitated in other relevant media: human serum, urine, and aqueous extract of ham. The level of toxin, from 5 to 200 ng/ml, can be accurately predicted in these media by calibrating each fiber and by comparing results to a single standard curve based on toxin in buffer. The quantitative fluorescent sandwich immunoassay provides results in 45 min; qualitative results are provided in 15-20 min. Using a blender and a benchtop centrifuge, fast, simple aqueous extracts of contaminated ham samples were prepared and tested. Ham spiked with 5 or 40 micrograms SEB per 100 g food resulted in biosensor readings indicative of 11 or 69% recovery of the toxin, respectively. Finally, the SEB assay is highly specific; SEA and SED give only 2-3% of the signal at 5000 ng/ml as SEB gives at 1000 ng/ml. This specific, sensitive assay for SEB on the portable fiber-optic biosensor permits easy monitoring of clinical samples or on-site analysis of suspect food samples.



This paper is referenced by
10.1016/J.SNB.2007.03.021
Detection of Staphylococcus enterotoxin B at picogram levels using piezoelectric-excited millimeter-sized cantilever sensors
Gossett A. Campbell (2007)
RAPTOR: A PORTABLE, AUTOMATED BIOSENSOR
G. P. Anderson (2000)
10.5812/jjm.17473
A Review Approaches to Identify Enteric Bacterial Pathogens
J. Amani (2015)
Raptor: A Portable Biosensor Upgraded for Reliability and Sensitivity
G. M. Anderson (2003)
10.4315/0362-028X-66.10.1851
Detection of staphylococcal enterotoxin B in spiked food samples.
L. Shriver-Lake (2003)
Design of fluorescence immunoassays. Perspectives for continuous monitoring of biological warface agents.
Laure-Marie Neuburger (2006)
10.1007/S12566-009-0006-X
Toxin immunosensors and sensor arrays for food quality control
Simone S. Moises (2009)
10.1364/OL.41.000100
Exciting fluorescence compounds on an optical fiber's side surface with a liquid core waveguide.
J. Ray (2016)
10.3389/fmicb.2018.00436
Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning
Emilie L Fisher (2018)
10.1039/c2an35471c
Ultrasensitive and selective homogeneous sandwich immunoassay detection by Surface Enhanced Raman Scattering (SERS).
Mustafa Ersin Pekdemir (2012)
Defense Technical Information Center Compilation Part Notice ADP 017722
L. Shriver-Lake ()
Detection of pathogenic bacteria and fecal enterococci in recreational water with an evanescent wave fiber optic biosensor
M. T. Trindade (2005)
10.3109/03639049809108575
Applications of fiber-optic evanescent wave spectroscopy.
E. Squillante (1998)
10.4315/0362-028X-64.1.37
Surface plasmon resonance analysis of staphylococcal enterotoxin B in food.
A. Rasooly (2001)
10.1109/JSEN.2003.815775
RAPTOR: a fluoroimmunoassay-based fiber optic sensor for detection of biological threats
C. Jung (2003)
10.1016/B978-044453125-4.50004-8
Chapter 2 – EVANESCENT WAVE FIBER OPTIC BIOSENSORS
G. Anderson (2008)
10.1016/S0956-5663(97)00070-5
Antibody immobilization using heterobifunctional crosslinkers.
L. Shriver-Lake (1997)
10.1016/S0956-5663(03)00128-3
Piezoelectric crystal immunosensor for the detection of staphylococcal enterotoxin B.
Huei-Cing Lin (2003)
10.1529/BIOPHYSJ.104.049684
Dielectrophoretic traps for single-particle patterning.
A. Rosenthal (2005)
Development of ganglioside-based assays for the identification of botulinum and cholera toxins utilizing an evanescent wave biosensor
Crystal M Bedenbaugh (2006)
Establishment of a multipurpose biologically based fiber optic immunosensor
N. Colls (2001)
10.1109/JPROC.2003.813574
Detection of microorganisms and toxins with evanescent wave fiber-optic biosensors
D. Lim (2003)
10.1128/AEM.70.10.6138-6146.2004
Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor
T. Geng (2004)
10.1002/1099-1352(200011/12)13:6<382::AID-JMR511>3.0.CO;2-W
Phage‐displayed peptides as biosensor reagents
E. Goldman (2000)
10.3390/S6080796
Fiber-Optic Biosensor Employing Alexa-Fluor Conjugated Antibody for Detection of Escherichia coli O157:H7 from Ground Beef in Four Hours
T. Geng (2006)
An anti-pyruvate kinase monoclonal antibody and translocated intimin receptor (tir) for specific detection of listeria species and shiga-toxigenic escherichia coli
Titiksha Dikshit (2013)
10.1002/9780470027318.A0206.PUB2
Fluorescence-based Biosensors
Michael Schäferling (2011)
10.1201/B13065-24
- Nano-Optical Sensors for Food Safety and Security
Euiwon Bae (2016)
10.1007/s13206-016-1201-9
Recent advances in the detection of methicillin resistant Staphylococcus aureus (MRSA)
John Hulme (2017)
10.1016/S0956-5663(99)00053-6
Multi-analyte interrogation using the fiber optic biosensor.
G. P. Anderson (2000)
10.3390/s100403351
Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray
Jason Wojciechowski (2010)
10.1080/10934529.2011.562810
Detection of staphylococcal enterotoxin A (SEA) at picogram level by a capacitive immunosensor
Jongjit Jantra (2011)
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