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Tools And Techniques For Chloroplast Transformation Of Chlamydomonas.

S. Purton
Published 2007 · Medicine, Biology

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The chloroplast organelle of plant and algal cells contains its own genetic system with a genome of a hundred or so genes. Stable transformation of the chloroplast was first achieved in 1988, using the newly developed biolistic method of DNA delivery to introduce cloned DNA into the genome of the green unicellular alga Chlamydomonas reinhardtii. Since that time there have been significant developments in chloroplast genetic engineering using this versatile organism, and it is probable that the next few years will see increasing interest in commercial applications whereby high-value therapeutic proteins and other recombinant products are synthesized in the Chlamydomonas chloroplast. In this chapter I review the basic methodology of chloroplast transformation, the current techniques and applications, and the future possibilities for using the Chlamydomonas chloroplast as a green organelle factory.
This paper references
10.1089/104454902762053891
Microparticle bombardment as a tool in plant science and agricultural biotechnology.
N. Taylor (2002)
10.1007/BF02172529
Selectable marker recycling in the chloroplast
N. Fischer (2005)
10.1146/ANNUREV.ARPLANT.55.031903.141633
Plastid transformation in higher plants.
P. Maliga (2004)
10.1023/B:PRES.0000030458.98624.37
Engineering the Chloroplast Encoded Proteins of Chlamydomonas
L. Xiong (2004)
10.1016/S0167-7799(00)01444-X
Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products.
L. Bogorad (2000)
10.1016/0076-6879(93)17087-L
Chloroplast transformation in Chlamydomonas.
J. Boynton (1993)
10.1007/978-94-007-1038-2
Photosynthesis in Algae
A. W. D. Larkum (2003)
10.1007/978-94-007-1038-2_5
Functional Analysis of Plastid Genes through Chloroplast Reverse Genetics in Chlamydomonas
Jean-David Rochaix (2003)
10.1128/MCB.15.6.3003
Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA.
H. Cerutti (1995)
10.1093/PCP/PCI157
Inducible trans-activation of plastid transgenes: expression of the R. eutropha phb operon in transplastomic tobacco.
A. Lössl (2005)
10.1046/J.1365-313X.2002.01319.X
Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast.
S. Franklin (2002)
10.1007/0-306-48204-5
The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas
Jean-David Rochaix (1998)
10.1023/A:1024140114505
Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast
M. Sun (2004)
10.1073/pnas.88.5.1721
Engineering the chloroplast genome: techniques and capabilities for chloroplast transformation in Chlamydomonas reinhardtii.
K. Kindle (1991)
10.1111/J.1745-7270.2005.00092.X
Recombination and heterologous expression of allophycocyanin gene in the chloroplast of Chlamydomonas reinhardtii.
Zhongliang Su (2005)
10.1016/S0014-5793(03)00034-6
Use of aminoglycoside adenyltransferase translational fusions to determine topology of thylakoid membrane proteins
J. L. Franklin (2003)
10.1016/0167-7799(88)90023-6
The biolistic process
J. Sanford (1988)
10.1093/nar/19.15.4083
Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker of site-directed transformation of chlamydomonas.
M. Goldschmidt-Clermont (1991)
10.1073/pnas.0237108100
Expression and assembly of a fully active antibody in algae
S. Mayfield (2003)
10.1073/pnas.90.2.497
In vivo analysis of Chlamydomonas chloroplast petD gene expression using stable transformation of beta-glucuronidase translational fusions.
W. Sakamoto (1993)
10.1007/0-306-48204-5_8
Chloroplast Transformation and Reverse Genetics
M. Goldschmidt-Clermont (1998)
10.1016/J.BBRC.2005.02.064
The Klebsiella pneumoniae nitrogenase Fe protein gene (nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii.
Q. Cheng (2005)
10.1007/s00438-005-0055-y
Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes
D. Barnes (2005)
10.1046/J.1365-313X.2002.01340.X
Searching limiting steps in the expression of chloroplast-encoded proteins: relations between gene copy number, transcription, transcript abundance and translation rate in the chloroplast of Chlamydomonas reinhardtii.
S. Eberhard (2002)
10.1126/science.2897716
Chloroplast transformation in Chlamydomonas with high velocity microprojectiles.
J. Boynton (1988)
10.1007/0-306-48127-8_32
Chloroplast Transformation: Current Results and Future Prospects
J. Erickson (1996)
10.1023/B:PRES.0000015384.24958.a9
Minimal Extent of Sequence Homology Required for Homologous Recombination at the psbA Locus in Chlamydomonas reinhardtii Chloroplasts using PCR-generated DNA Fragments
D. Dauvillée (2004)
10.1111/J.1365-313X.2005.02495.X
External control of transgene expression in tobacco plastids using the bacterial lac repressor.
S. Mühlbauer (2005)
10.1105/tpc.011692
Cytochrome f Translation in Chlamydomonas Chloroplast Is Autoregulated by its Carboxyl-Terminal Domain Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.011692.
Y. Choquet (2003)
10.1007/s004380051101
Renilla luciferase as a vital reporter for chloroplast gene expression in Chlamydomonas
I. Min'ko (1999)
10.1073/pnas.081078898
Evidence for two active branches for electron transfer in photosystem I
M. Guergova-Kuras (2001)
10.1016/S1389-1723(03)80030-0
Introduction of the archaebacterial geranylgeranyl pyrophosphate synthase gene into Chlamydomonas reinhardtii chloroplast.
E. Fukusaki (2003)
10.1016/S1389-1723(99)80037-1
Expression of a foreign gene in Chlamydomonas reinhardtii chloroplast.
K. Ishikura (1999)
10.1073/pnas.052327599
Evidence for in vivo modulation of chloroplast RNA stability by 3′-UTR homopolymeric tails in Chlamydomonas reinhardtii
Y. Komine (2002)
10.1046/J.1365-313X.2003.01965.X
Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast.
S. Mayfield (2004)
10.1074/jbc.270.16.9651
A Mutant Strain of Chlamydomonas reinhardtii Lacking the Chloroplast Photosystem II psbI Gene Grows Photoautotrophically (*)
P. Künstner (1995)
10.1111/J.1529-8817.2005.00133.X
Algal transgenics in the genomic ERA
T. Walker (2005)
10.1517/14712598.5.2.225
Recent developments in the production of human therapeutic proteins in eukaryotic algae
S. Franklin (2005)
10.1007/0-306-48204-5_12
Regulation of Chloroplast Translation
C. Hauser (1998)
10.1046/J.1529-8817.1999.3520215.X
COMMERCIAL DEVELOPMENTS IN MICROALGAL BIOTECHNOLOGY
K. Apt (1999)
10.1007/s004380051184
Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker
J. M. Bateman (2000)
10.1016/S0378-1119(03)00754-6
DNA transfer from chloroplast to nucleus is much rarer in Chlamydomonas than in tobacco.
D. L. Lister (2003)
10.1007/978-94-011-2656-4_10
The chloroplast genome.
M. Sugiura (1989)
10.1146/annurev.ge.29.120195.001233
Chlamydomonas reinhardtii as the photosynthetic yeast.
J. Rochaix (1995)
10.1007/0-306-48127-8
Oxygenic Photosynthesis: The Light Reactions
D. Ort (1996)
10.1016/J.PBI.2004.01.012
Prospects for molecular farming in the green alga Chlamydomonas.
S. Franklin (2004)



This paper is referenced by
PROGRAMA DE POSGRADO EN ALIMENTOS DEL CENTRO DE LA REPUBLICA (PROPAC) Maestría en Ciencia y Tecnología de Alimentos
F. Química (2008)
10.1007/s11274-018-2567-8
Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory
Yong Min Kwon (2018)
10.1099/mic.0.000910
Multigenic engineering of the chloroplast genome in the green alga Chlamydomonas reinhardtii
Marco Larrea-Álvarez (2020)
10.1128/EC.00108-09
The ARG9 Gene Encodes the Plastid-Resident N-Acetyl Ornithine Aminotransferase in the Green Alga Chlamydomonas reinhardtii
C. Remacle (2009)
10.1007/978-1-4939-2493-6_436-3
Algae, a New Biomass Resource
Alberta Pinnola (2017)
10.1016/j.algal.2019.101662
N-terminal sequences affect expression of triterpene biosynthesis enzymes in Chlamydomonas chloroplasts
Shih-Chi Hsu (2019)
MAKING CHLAMYDOMONAS REINHARDTII A BETTER MODEL ORGANISM: TACKLING THE INEFFICIENCY OF NUCLEAR TRANSGENE EXPRESSION AND IMPROVING METHODS FOR THE GENERATION AND CHARACTERIZATION OF INSERTIONAL MUTANT LIBRARIES
Thomas M Plucinak (2013)
10.1002/9781118567166.CH21
Novel Sulfated Polysaccharides of Red Microalgae: Basics and Applications
Shoshana Arad (2013)
Recombinant protein production in the chloroplast of microalgae: a systems biology approach
Oluwafemi Davies (2015)
10.1007/s00253-016-7354-6
New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii
T. Wannathong (2016)
10.1111/pbi.12073
Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii.
Hsu-Ching Chen (2013)
10.1007/978-1-4939-1136-3_18
Synthesis of Recombinant Products in the Chloroplast
Ghislaine Tissot-Lecuelle (2014)
10.3390/ijms14034734
Low Oxygen Response Mechanisms in Green Organisms
Valeria Banti (2013)
Metabolic engineering of the algal chloroplast for terpenoid production
Uha Al Hoqani (2017)
10.2791/3339
Microalgae-based products for the food and feed sector: an outlook for Europe
C. Enzing (2014)
10.1111/tpj.13173
Extending the biosynthetic repertoires of cyanobacteria and chloroplasts.
A. Z. Nielsen (2016)
Genetic engineering of green microalgae for the production of biofules and high value products
J. Szaub (2013)
Algues génétiquement modifiées résistant aux herbicides
Su-Chiung Fang (2009)
Expression of the bacterial ectC gene in the chloroplast of Chlamydomonas reinhardtii
Victoria Teigland Holck (2014)
10.1242/jcs.175232
Localized control of oxidized RNA
Y. Zhan (2015)
10.1007/978-94-007-2920-9_16
Plastid Transformation in Algae
Jörg Nickelsen (2012)
The Biogenesis of Photosynthetic Complexes PSII and b6f
Sara Cline (2012)
10.1002/biot.201400566
Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid Verde Fluorescent Protein as a reporter
Stephanie Braun-Galleani (2015)
The ARG 9 Gene Encodes the Plastid-Resident N-Acetyl Ornithine Aminotransferase in the Green Alga Chlamydomonas reinhardtii
Claire Remacle (2009)
10.17863/CAM.25128
Exploring the potential of algae-bacteria communities for biotechnology
Anthony S Riseley (2018)
1. Improving the yield of biodiesel from microalgae and other lipids. 2. Studies of the wax ester biosynthetic pathway and potential biotechnological application
Bradley D. Wahlen (2012)
10.1007/978-90-481-3795-4_11
Red Microalgae: From Basic Know-How to Biotechnology
Miri Lapidot (2010)
10.1016/J.IJHYDENE.2011.05.130
Introducing pyruvate oxidase into the chloroplast of Chlamydomonas reinhardtii increases oxygen cons
Fu-Qiao Xu (2011)
10.3389/fpls.2016.00505
Transgene Expression in Microalgae—From Tools to Applications
Lior Doron (2016)
10.1099/mic.0.000599
The algal chloroplast as a synthetic biology platform for production of therapeutic proteins.
Y. M. Dyo (2018)
10.1111/tpj.12675
Cytosine deaminase as a negative selectable marker for the microalgal chloroplast: a strategy for the isolation of nuclear mutations that affect chloroplast gene expression
Rosanna E. B. Young (2014)
10.31618/esu.2413-9335.2019.3.59.37-48
Insight into crispr system in eukaryotic microalgae, a review
Guénole-Bilal Issam (2019)
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