In Vivo Cellular Imaging Using Fluorescent Proteins
Published 2012 · Chemistry
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Cell migration and metastasis are key features of aggressive tumors. These processes can be diffi cult to study, as they often occur deep within the body of a cancer patient or an experimental animal. In vitro assays are able to model some aspects of these processes, and a number of assays have been developed to assess cancer cell motility, migration, and invasion. However, in vitro assays have inherent limitations that may miss important aspects of these processes as they occur in vivo. The chick embryo provides a powerful model for studying these processes in vivo, facilitated by the external and accessible nature of the chorioallantoic membrane (CAM), a well-vascularized tissue that surrounds the embryo. When coupled with multiple fl uorescent approaches to labeling both cancer cells and the embryonic vasculature, along with image analysis tools, the chick CAM model offers cost-effective, rapid assays for studying cancer cell migration and metastasis in a physiologically-relevant, in vivo setting. Here, we present recent developments of detailed procedures for using shell-less chick embryos, coupled with fl uorescent labeling of cancer cells and/or chick vasculature, to study cancer cell migration and metastasis in vivo.
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Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virus.
Q. Zhang (2007)
Generation of donor hematolymphoid cells after rat-limb composite grafting
T. Ajiki (2003)
Cytoreductive surgery and peri-operative intraperitoneal chemotherapy as a curative approach to pseudomyxoma peritonei syndrome.
P. Sugarbaker (2001)
Green fluorescent protein-transgenic rat: a tool for organ transplantation research.
Y. Hakamata (2001)
Dynamic Imaging of T Cell-Dendritic Cell Interactions in Lymph Nodes
S. Stoll (2002)
In vitro and in vivo transfection efficiency of a novel ultradeformable cationic liposome.
A. Kim (2004)
GFP-transgenic Lewis rats as a cell source for oligodendrocyte replacement
J. Francis (2007)
The multiple uses of fl
R. M. Hoffman (2005)
Production of knockdown rats by lentiviral transduction of embryos with short hairpin RNA transgenes.
C. Dann (2008)
MicroRNAs Genomics, Biogenesis, Mechanism, and Function
D. Bartel (2004)
NATURAL ANIMAL COLORATION CAN BE DETERMINED BY A NON-FLUORESCENT GFP HOMOLOG
K. Lukyanov (2001)
Colloid chemistry. Liposomes within liposomes.
D. Lasič (1997)
Orthotopic Transplant Mouse Models with Green Fluorescent Protein-expressing Cancer Cells to Visualize Metastasis and Angiogenesis
R. Hoffman (2004)
Live imaging of blood
I. V. Larina (2008)
Genetic Variants and Strains of the Laboratory Mouse 3rd edn
M. F. Lyon (1996)
Developmental imaging: Insights into the avian embryo.
P. Kulesa (2004)
Red-Shifted Excitation Mutants of the Green Fluorescent Protein
S. Delagrave (1995)
Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors.
M. Studeny (2002)
Antagonism of Sphingosine 1-Phosphate Receptor-2 Enhances Migration of Neural Progenitor Cells Toward an Area of Brain Infarction
A. Kimura (2008)
Oncolytic vaccinia virotherapy of anaplastic thyroid cancer in vivo.
Shu-Fu Lin (2008)
Telomerase: a target for cancer therapeutics.
J. Shay (2002)
Computationally designed libraries for rapid enzyme stabilization
H. Wijma (2014)
In vivo spectral fluorescence imaging of submillimeter peritoneal cancer implants using a lectin-targeted optical agent.
Y. Hama (2006)
Liposomes: the development of a new carrier system for introducing nucleic acid into plant and animal cells.
R. Fraley (1982)
Homing and cellular traffic in lymph nodes
U. V. Andrian (2003)
A critical step in metastasis: in vivo analysis of intravasation at the primary tumor.
J. Wyckoff (2000)
Laparoscopy for Malignancy: Current Status
P. Paraskeva (2004)
Stem cells: roadmap to the clinic.
G. Daley (2010)
Intussusceptive angiogenesis: Its emergence, its characteristics, and its significance
P. Burri (2004)
Genetically encoded fl
M. Prescott (2008)
Improved green fl
R. Heim (1995)
Mesenchymal stem cell homing: the devil is in the details.
J. Karp (2009)
Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area.
K. Tsuji (2006)
In vivo internal tumor illumination by telomerase-dependent adenoviral GFP for precise surgical navigation
H. Kishimoto (2009)
EXPRESSION OF PORCINE GROWTH HORMONE GENE IN TRANSGENIC RABBITS AS REPORTED BY GREEN FLUORESCENT PROTEIN
H. Wang (2001)
The purification fo four strains of poxvirus.
W. Joklik (1962)
In vivo imaging of lymphocyte trafficking.
C. Halin (2005)
Generation of Fertile Cloned Rats by Regulating Oocyte Activation
Q. Zhou (2003)
Normalization of Tumor Vasculature: An Emerging Concept in Antiangiogenic Therapy
R. Jain (2005)
In vivo molecular imaging to diagnose and subtype tumors through receptor-targeted optically labeled monoclonal antibodies.
Y. Koyama (2007)
FACS-optimized mutants of the green fluorescent protein (GFP).
B. Cormack (1996)
Oncolytic herpes simplex virus-1 mutant expressing green fluorescent protein can detect and treat peritoneal cancer.
S. F. Stanziale (2004)
Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein.
R. Wachter (1998)
Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system.
K. Yamauchi (2006)
Time-dependent density-functional approach for biological chromophores: the case of the green fluorescent protein.
M. A. Marques (2003)
Whole-body imaging with fluorescent proteins
R. Hoffman (2006)
Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa.
S. Habuchi (2005)
Advantages of multi-color fluorescent proteins for whole-body and in vivo cellular imaging.
R. Hoffman (2005)
Hemodynamic measurements from individual blood cells in early mammalian embryos with Doppler swept source OCT.
I. Larina (2009)
Hair follicle-derived blood vessels vascularize tumors in skin and are inhibited by Doxorubicin.
Y. Amoh (2005)
Whole-body multicolor spectrally resolved fluorescence imaging for development of target-specific optical contrast agents using genetically engineered probes
Hisataka Kobayashi (2007)
Macrophages promote the invasion of breast carcinoma cells via a colony-stimulating factor-1/epidermal growth factor paracrine loop.
S. Goswami (2005)
Real-time diagnostic imaging with a novel x-ray detector with multiple screen-CCD sensors
C. Slump (1998)
mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures
Simone Kredel (2009)
Determination of clonality of metastasis by cell-specific color-coded fluorescent-protein imaging.
N. Yamamoto (2003)
Marked prolongation of porcine renal xenograft survival in baboons through the use of α1,3-galactosyltransferase gene-knockout donors and the cotransplantation of vascularized thymic tissue
K. Yamada (2005)
Transgene expression of green fluorescent protein and germ line transmission in cloned pigs derived from in vitro transfected adult fibroblasts.
D. Brunetti (2008)
THE MOLECULAR STRUCTURE OF GREEN FLUORESCENT PROTEIN
Development of new inbred transgenic strains of rats with LacZ or GFP.
H. Inoue (2005)
Molecular basis and evolutionary origins of color diversity in great star coral Montastraea cavernosa (Scleractinia: Faviida).
I. V. Kelmanson (2003)
Role of Angiogenesis in Human Tumor Dormancy: Animal Models of the Angiogenic Switch
G. Naumov (2006)
MicroRNAs as biomarkers and therapeutic drugs in human cancer.
M. Osaki (2008)
Escherichia coli Nissle 1917 Facilitates Tumor Detection by Positron Emission Tomography and Optical Imaging
P. Brader (2008)
In vivo molecular imaging of cancer with a quenching near-infrared fluorescent probe using conjugates of monoclonal antibodies and indocyanine green.
Mikako Ogawa (2009)
Double-Stranded RNA as a Template for Gene Silencing
B. Bass (2000)
Combinational antitumor effect of siRNA against midkine and paclitaxel on growth of human prostate cancer xenografts
Y. Takei (2006)
Use of green
L. Cheng (1996)
Rapid maturing red fl
M. Sorensen (2003)
Indications for pneumonectomy. Extrapleural pneumonectomy.
V. Rusch (1999)
Increased transgene integration efficiency upon microinjection of DNA into both pronuclei of rabbit embryos
P. Chrenek (2005)
Current methods for assaying angiogenesis in vitro and in vivo.
C. Staton (2004)
Fluorescent pigments in corals are photoprotective
A. Salih (2000)
Advances in imaging mouse tumour models in vivo.
S. Lyons (2005)
Telomerase: regulation, function and transformation.
C. Dong (2005)
Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations.
J. Felgner (1994)
Fluorescence lifetime imaging of coral fluorescent proteins.
G. Cox (2007)
D-galactose receptor-targeted in vivo spectral fluorescence imaging of peritoneal metastasis using galactosamin-conjugated serum albumin-rhodamine green.
Y. Hama (2007)
Multi-targeted multi-color in vivo optical imaging in a model of disseminated peritoneal ovarian cancer.
N. Kosaka (2009)
A guide to choosing fluorescent proteins
Nathan Shaner (2005)
MicroRNAs in carcinogenesis
J. Hagan (2007)
Noninvasive imaging of spontaneous retinoblastoma pathway-dependent tumors in mice.
M. Vooijs (2002)
Cellular Dynamics Visualized in Live Cells in Vitro and in Vivo by Differential Dual-Color Nuclear-Cytoplasmic Fluorescent-Protein Expression
N. Yamamoto (2004)
The use of histoculture and green
T. Chishima (1997)
Synthetic control of green fluorescent protein.
K. P. Kent (2009)
Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats.
K. Takeuchi (2003)
A novel gene therapy to the graft organ by a rapid injection of naked DNA I: long-lasting gene expression in a rat model of limb transplantation
Y. Sato (2003)
Effective treatment of pancreatic tumors with two multimutated herpes simplex oncolytic viruses
P. McAuliffe (2000)
A redshifted codon-optimized firefly luciferase is a sensitive reporter for bioluminescence imaging.
H. Caysa (2009)
Oncolytic herpesvirus effectively treats murine squamous cell carcinoma and spreads by natural lymphatics to treat sites of lymphatic metastases.
R. Wong (2002)
Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.
Y. Sato (2003)
Development of liver regenerative therapy using glycoside-modified bone marrow cells.
R. Misawa (2006)
Optical imaging: bacteria, viruses, and mammalian cells encoding light-emitting proteins reveal the locations of primary tumors and metastases in animals
Y. A. Yu (2003)
Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice.
S. Patwardhan (2005)
Sentinel node biopsy in breast cancer: early results in 953 patients with negative sentinel node biopsy and no axillary dissection.
U. Veronesi (2005)
Multicolor in vivo targeted imaging to guide real‐time surgery of HER2‐positive micrometastases in a two‐tumor coincident model of ovarian cancer
M. Longmire (2009)
Amplification of cDNA ends based on template-switching effect and step-out PCR.
M. Matz (1999)
Polysurgery of cell sheet grafts overcomes diffusion limits to produce thick, vascularized myocardial tissues
T. Shimizu (2006)
Transgenic Nude Mouse with Ubiquitous Green Fluorescent Protein Expression as a Host for Human Tumors
M. Yang (2004)
GFP: from jellyfi
M. Zimmer (2009)
Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling.
W. Wang (2002)
In-vivo confocal scanning laser microscopy of the cerebral microcirculation.
U. Dirnagl (1992)
Development and Use of Fluorescent Protein Markers in Living Cells
J. Lippincott-Schwartz (2003)
The neuronal naturalist: watching neurons in their native habitat
J. Lichtman (2001)
Rapid and quantitative assessment of cancer treatment response using in vivo bioluminescence imaging.
A. Rehemtulla (2000)
Colonization of experimental murine breast tumours by Escherichia coli K‐12 significantly alters the tumour microenvironment
S. Weibel (2008)
Mutational Analysis of Deinococcus radiodurans Bacteriophytochrome Reveals Key Amino Acids Necessary for the Photochromicity and Proton Exchange Cycle of Phytochromes*
J. Wagner (2008)
Bioluminescent indicators in living mammals
P. R. Contag (1998)
A clinical scoring system predicts the yield of diagnostic laparoscopy in patients with potentially resectable hepatic colorectal metastases
W. Jarnagin (2001)
Structure, dynamics and optical properties of fl
G. U. Nienhaus (2009)
Major colour patterns of reef-building corals are due to a family of GFP-like proteins
S. Dove (2001)
Cellular organization and spectral diversity of GFP-like proteins in live coral cells studied by single and multiphoton imaging and microspectroscopy
A. Salih (2003)
Improved green fluorescence
R. Heim (1995)
Fluorescent proteins from nonbioluminescent Anthozoa species
M. Matz (1999)
Orthotopic metastatic (MetaMouse) models for discovery and development of novel chemotherapy.
R. Hoffman (2005)
The Role of Staging Laparoscopy in Hepatobiliary Malignancy: Prospective Analysis of 401 Cases
M. D'angelica (2003)
Effective therapy for peritoneal dissemination in gastric cancer.
Y. Yonemura (2003)
Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo
K. J. Kim (1993)
Clinical trial results with oncolytic virotherapy: a century of promise, a decade of progress
T. Liu (2007)
Vascular endothelial growth factor is a secreted angiogenic mitogen.
D. W. Leung (1989)
Establishment of red fluorescent protein-tagged HeLa tumor metastasis models: Determination of DsRed2 insertion effects and comparison of metastatic patterns after subcutaneous, intraperitoneal, or intravenous injection
Jau-Yeong Lu (2004)
Endogenous green fl uorescent protein (GFP) in amphioxus
N. D. Holland (2007)
Looking and listening to light: the evolution of whole-body photonic imaging
V. Ntziachristos (2005)
Establishment and characterization of conditions required for tumor colonization by intravenously delivered bacteria
Y. A. Yu (2008)
Imaging in the era of molecular oncology
R. Weissleder (2008)
A Role for VEGF as a Negative Regulator of Pericyte Function and Vessel Maturation
J. Greenberg (2008)
Cell motility of tumor cells visualized in living intact primary tumors using green fluorescent protein.
K. Farina (1998)
Pulsatile Myocardial Tubes Fabricated With Cell Sheet Engineering
H. Sekine (2006)
Technicolour transgenics: imaging tools for functional genomics in the mouse
A. Hadjantonakis (2003)
Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications.
B. Branchini (2007)
Non‐Retinal Chromophoric Proteins
M. Zimmer (2006)
The replication-competent oncolytic herpes simplex mutant virus NV1066 is effective in the treatment of esophageal cancer.
B. Stiles (2003)
Treatment of Malignant Mesothelioma
M. Jaklitsch (2001)
Dynamic in vivo imaging of postimplantation mammalian embryos using whole embryo culture.
E. A. Jones (2002)
Contributions of host and symbiont pigments to the coloration of reef corals
F. Oswald (2007)
Dynamic Stokes shift in green fluorescent protein variants
Paul Abbyad (2007)
A transgenic red fluorescent protein-expressing nude mouse for color-coded imaging of the tumor microenvironment.
M. Yang (2009)
Vascular Normalization by Antiangiogenic Agents Time-Course Imaging of Therapeutic Functional Tumor
Qingbei Zhang (2011)
Enhanced green fl
J. W. Sturm (2003)
Intra-articular injected synovial stem cells differentiate into meniscal cells directly and promote meniscal regeneration without mobilization to distant organs in rat massive meniscal defect
堀江 雅史 (2009)
Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling
A. Crameri (1996)
Improving diagnostic staging laparoscopy using intraperitoneal lavage of delta-aminolevulinic acid (ALA) for laparoscopic fluorescence diagnosis.
J. Gahlen (1999)
The Hallmarks of Cancer
D. Hanahan (2000)
Intravital imaging of cell movement in tumours
J. Condeelis (2003)
In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy.
K. H. Al-Gubory (2006)
Imaging a Genetically Engineered Oncolytic Vaccinia Virus (GLV-1h99) Using a Human Norepinephrine Transporter Reporter Gene
P. Brader (2009)
Innovation: Photoactivatable fluorescent proteins.
K. Lukyanov (2005)
Angiogenesis: Tumorigenesis and the angiogenic switch
G. Bergers (2003)
A survey of telomerase activity in human cancer.
J. Shay (1997)
Optimizing imaging parameters for the separation of multiple labels in a fluorescence image.
R. Neher (2004)
Visualization of early events in tumor formation of eGFP-transfected rat colon cancer cells in liver.
O. Mook (2003)
Lymphatic mapping and sentinel lymphadenectomy for breast cancer.
M. Kelley (2004)
Mesenchymal stem cells: potential precursors for tumor stroma and targeted-delivery vehicles for anticancer agents.
M. Studeny (2004)
A Membrane Associated mCherry Fluorescent Reporter Line for Studying Vascular Remodeling and Cardiac Function During Murine Embryonic Development
I. Larina (2009)
Imaging angiogenesis and the microenvironment
D. Fukumura (2008)
In vivo real‐time imaging of nuclear‐cytoplasmic dynamics of dormancy, proliferation and death of cancer cells
R. Hoffman (2008)
Transfection by Cationic Liposomes Using Simultaneous Single Cell Measurements of Plasmid Delivery and Transgene Expression*
W. Tseng (1997)
Characterization of hair follicles induced in implanted, cultured rat keratinocyte sheets
H. Miyashita (2004)
Targeting the molecular target of rapamycin (mTOR)
E. Rowinsky (2004)
Phytochrome photosensory signalling networks
P. Quail (2002)
In vivo imaging of lymph node metastasis with telomerase-specific replication-selective adenovirus
H. Kishimoto (2006)
Indian hedgehog activates hematopoiesis and vasculogenesis and can respecify prospective neurectodermal cell fate in the mouse embryo.
M. Dyer (2001)
RPN2 gene confers docetaxel resistance in breast cancer
K. Honma (2008)
Renilla luciferase-Aequorea GFP (Ruc-GFP) fusion protein, a novel dual reporter for real-time imaging of gene expression in cell cultures and in live animals
Y. Wang (2002)
A phase II trial of surgical resection and adjuvant high-dose hemithoracic radiation for malignant pleural mesothelioma.
V. Rusch (2001)
Cloned rabbits produced by nuclear transfer from adult somatic cells
P. Chesné (2002)
Diversity and Evolution of Coral Fluorescent Proteins
N. Alieva (2008)
Pharmacologic inactivation of kinase suppressor of ras-1 abrogates Ras-mediated pancreatic cancer
H. Xing (2003)
Real-time imaging of lymphogenic metastasis in orthotopic human breast cancer.
M. Dadiani (2006)
Epigenetic reprogramming in embryonic and foetal development upon somatic cell nuclear transfer cloning.
H. Niemann (2008)
Bone marrow cells differentiate into wound myofibroblasts and accelerate the healing of wounds with exposed bones when combined with an occlusive dressing
Y. Yamaguchi (2005)
Vascular remodeling of the mouse yolk sac requires hemodynamic force
Jennifer L. Lucitti (2007)
T KaoH. (2007)
Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases
A. Geurts (2009)
Effective treatment of head and neck squamous cell carcinoma by an oncolytic herpes simplex virus.
R. Wong (2001)
Herpes viral oncolysis: a novel cancer therapy.
M. Reinblatt (2007)
Lympho-myeloid chimerism achieved by spleen graft of green fluorescent protein transgenic rat in a combined pancreas transplantation model (TI03-029).
Y. Sakuma (2004)
Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels.
K. Hayashi (2007)
Whole-body subcellular multicolor imaging of tumor-host interaction and drug response in real time.
M. Yang (2007)
Orthotopic Metastatic Mouse Models for Anticancer Drug Discovery and Evaluation: a Bridge to the Clinic
R. Hoffman (2004)
Novel oncolytic agent GLV-1h68 is effective against malignant pleural mesothelioma.
K. Kelly (2008)
Tumor-targeting amino acid auxotrophic Salmonella typhimurium
R. Hoffman (2009)
Evaluation and optimization of DNA delivery into gliosarcoma 9L cells by a cholesterol-based cationic liposome.
D. Lesage (2002)
An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
R. Ando (2002)
GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity.
D. Shagin (2004)
Regression of human pancreatic tumor xenografts in mice after a single systemic injection of recombinant vaccinia virus GLV-1h68
Y. A. Yu (2009)
Virally directed fluorescent imaging improves diagnostic sensitivity in the detection of minimal residual disease after potentially curative cytoreductive surgery
P. Adusumilli (2005)
Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases.
M. Yang (2000)
Using a histone yellow
S. T. Fraser (2005)
Trends in large-scale mouse mutagenesis: from genetics to functional genomics
Y. Gondo (2008)
Performance of the red-shifted fluorescent proteins in deep-tissue molecular imaging applications.
N. Deliolanis (2008)
Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.
C. Cody (1993)
Progress towards in Vivo Use of siRNAs
M. Behlke (2006)
Endothelial Cell Coculture Within Tissue-Engineered Cardiomyocyte Sheets Enhances Neovascularization and Improves Cardiac Function of Ischemic Hearts
H. Sekine (2008)
Comparison of safety, delivery, and efficacy of two oncolytic herpes viruses (G207 and NV1020) for peritoneal cancer
J. Bennett (2002)
The multiple uses of fluorescent proteins to visualize cancer in vivo
R. Hoffman (2005)
Essential roles of sphingosine 1-phosphate/S1P1 receptor axis in the migration of neural stem cells toward a site of spinal cord injury.
A. Kimura (2007)
Targeting angiogenesis: progress with anti-VEGF treatment with large molecules
A. Grothey (2009)
Elimination of internal restriction enzyme sites from a bacterial luminescence (luxCDABE) operon.
C. Voisey (1998)
Antisense-based cancer therapeutics: are we there yet?
E. Rayburn (2006)
The first molecular details of ALT in human tumor cells.
A. Muntoni (2005)
Evaluation of chemosensitivity of micrometastases with green fluorescent protein gene-tagged tumor models in mice.
H. Nakanishi (2005)
Fluorescence diffuse tomography for detection of red fluorescent protein expressed tumors in small animals.
Ilya V. Turchin (2008)
Transgenic Modifications of the Rat Genome
L. Tesson (2005)
Imaging the fl
T. D. Ainsworth (2008)
Systemic delivery of miR-16 for RNAi therapy in prostate cancer
F. Takeshita (2010)
Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats.
H. Inoue (2008)
Important prognostic factors in patients with malignant pleural mesothelioma, managed surgically.
V. Rusch (1999)
In vivo imaging of metastatic cancer with fluorescent proteins
R. Hoffman (2002)
The highly attenuated oncolytic recombinant vaccinia virus GLV-1h68: comparative genomic features and the contribution of F14.5L inactivation
Q. Zhang (2009)
The structure of the chromophore within DsRed, a red fluorescent protein from coral.
Lydwine Gross (2000)
Visualization of tumors and metastases in live animals with bacteria and vaccinia virus encoding light-emitting proteins
Y. A. Yu (2004)
Bioluminescence in the sea.
S. H. Haddock (2010)
Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters.
F. Jöbsis (1977)
Fluorescence census techniques for the early detection of coral recruits
A. Baird (2005)
The silent treatment: siRNAs as small molecule drugs
D. Dykxhoorn (2006)
Cationic lipid-DNA complexes-lipoplexes-for gene transfer and therapy.
R. Zhdanov (2002)
Gene silencing in mammals by small interfering RNAs
Michael T. McManus (2002)
Activation of telomerase in human lymphocytes and hematopoietic progenitor cells.
K. Hiyama (1995)
In vivo color-coded imaging of the interaction of colon cancer cells and splenocytes in the formation of liver metastases.
M. Bouvet (2006)
Biological properties of coral GFP-type proteins provide clues for engineering novel optical probes and biosensors
A. Salih (2004)
Human melanoma cellular line mel Kor used in preparing antitu
I. N. Mikhajlova (2006)
Shedding light onto live molecular targets
R. Weissleder (2003)
Developments in transgenic technology: applications for medicine.
C. V. Hunter (2005)
Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome
X. Shu (2009)
Mesenchymal Stromal Cells: Current Understanding and Clinical Status
H. Salem (2010)
A monomeric red fluorescent protein
R. E. Campbell (2002)
The prospect of silencing disease using RNA interference.
P. Shankar (2005)
Real-time imaging of single cancer-cell dynamics of lung metastasis.
H. Kimura (2010)
Establishment and characterization of CAG/EGFP transgenic rabbit line
R. Takahashi (2006)
UV light killing effi cacy of fl
H. Kimura (2010)
Wavelength mutations and posttranslational autoxidation of green fluorescent protein.
R. Heim (1994)
Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction.
Y. Y. Zhu (2001)
Spectral FluorescenceMolecular Imaging of LungMetastases Targeting HER 2 / neu
Y. Koyama (2007)
Pathological angiogenesis is induced by sustained Akt signaling and inhibited by rapamycin.
Thuy L. Phung (2006)
Bioluminescent imaging (BLI) to improve and refine traditional murine models of tumor growth and metastasis
D. Jenkins (2004)
Clinical and pathologic features of proximal biliary strictures masquerading as hilar cholangiocarcinoma.
C. Corvera (2005)
Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex.
D. Kleinfeld (1998)
Crystal structure and photodynamic behavior of the blue emission variant Y66H/Y145F of green fluorescent protein.
R. Wachter (1997)
Intraoperative localization of lymph node metastases with a replication-competent herpes simplex virus.
P. Adusumilli (2006)
Structural basis for red‐shifted emission of a GFP‐like protein from the marine copepod Chiridius poppei
K. Suto (2009)
Cellular expression of green fluorescent protein, coupled with high-resolution in vivo videomicroscopy, to monitor steps in tumor metastasis.
G. Naumov (1999)
Plasmid DNA entry into postmitotic nuclei of primary rat myotubes.
M. Dowty (1995)
Metastasis: Dissemination and growth of cancer cells in metastatic sites
A. Chambers (2002)
Real-time in vivo dual-color imaging of intracapillary cancer cell and nucleus deformation and migration
山内 健輔 (2006)
Managing the peritoneal surface component of gastrointestinal cancer. Part 2. Perioperative intraperitoneal chemotherapy.
P. Sugarbaker (2004)
Simultaneous Time Resolution of the Emission Spectra of Fluorescent Proteins and Zooxanthellar Chlorophyll in Reef-building Corals¶,†
A. Gilmore (2003)
Transgenic animal models in biomedical research.
L. Houdebine (2007)
Clinical translation of angiogenesis inhibitors
R. Kerbel (2002)
Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression.
T. Chishima (1997)
Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis
A. Al-mehdi (2000)
Photoinduced activation of GFP-like proteins in tissues of reef corals
A. Salih (2006)
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
M. Hofmann (2005)
Cardiomyocyte bridging between hearts and bioengineered myocardial tissues with mesenchymal transition of mesothelial cells.
H. Sekine (2006)
Diversity and evolution of the green fluorescent protein family
Y. A. Labas (2002)
Real-time intraoperative detection of melanoma lymph node metastases using recombinant vaccinia virus GLV-1h68 in an immunocompetent animal model.
K. Kelly (2009)
Metastatic patterns of lung cancer visualized live and in process by green fluorescence protein expression
T. Chishima (2004)
The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins
V. Verkhusha (2004)
Association of MicroRNA Expression in Hepatocellular Carcinomas with Hepatitis Infection, Cirrhosis, and Patient Survival
J. Jiang (2008)
Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression
Asako Sakaue-Sawano (2008)
Determinants of Rapamycin Sensitivity in Breast Cancer Cells
Woo-Chul Noh (2004)
Long-Term Survival of Peritoneal Carcinomatosis of Colorectal Origin
V. Verwaal (2004)
In vivo bioimaging using photogenic rats: fate of injected bone marrow-derived mesenchymal stromal cells.
M. Hara (2008)
X. Jiao (2002)
Heart transplantation in baboons using α1,3-galactosyltransferase gene-knockout pigs as donors: initial experience
K. Kuwaki (2005)
Primary structure of the Aequorea victoria green-fluorescent protein.
D. Prasher (1992)
Energy transfer in a bioluminescent system.
J. Morin (1971)
Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source.
T. Kawarasaki (2009)
Fluorescent protein tomography scanner for small animal imaging
G. Zacharakis (2005)
Color-engineered rats and luminescent LacZ imaging: a new platform to visualize biological processes.
T. Murakami (2005)
Oncolytic vaccinia therapy of squamous cell carcinoma
Z. Yu (2009)
Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy
M. Miller (2003)
A Novel Recombinant Vaccinia Virus Expressing the Human Norepinephrine Transporter Retains Oncolytic Potential and Facilitates Deep-Tissue Imaging
N. Chen (2009)
Fluorescence molecular tomography resolves protease activity in vivo
V. Ntziachristos (2002)
‘Green mice’ as a source of ubiquitous green cells
M. Okabe (1997)
Induction of cancer metastasis by cyclophosphamide pretreatment of host mice: an opposite effect of chemotherapy.
K. Yamauchi (2008)
A color‐coded orthotopic nude‐mouse treatment model of brain‐metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis
K. Hayashi (2009)
Establishment of lacZ-transgenic rats: a tool for regenerative research in myocardium.
M. Takahashi (2003)
Fluorescent characteristics of macerals by fluorescent visual kerogen method
A. Omura (2000)
S. Zolotukhin (1996)
Visualization of intrathoracically disseminated solid tumors in mice with optical imaging by telomerase-specific amplification of a transferred green fluorescent protein gene
梅岡 達生 (2004)
Establishment of fl
M. S. Huang (2002)
Cellular Imaging Using Fluorescent Proteins: Methods and Protocols
Robert M. Hoffman ed (2012)
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells
A. Mothe (2005)
Phytochrome structure and signaling mechanisms.
N. Rockwell (2006)
Infection with Oncolytic Herpes Simplex Virus-1 Induces Apoptosis in Neighboring Human Cancer Cells
S. F. Stanziale (2004)
Endogenous green fluorescent protein (GFP) in amphioxus.
D. Deheyn (2007)
RNA interference: From gene silencing to gene-specific therapeutics
Ray K. M. Leung (2005)
Advances in oncolytic viral therapy.
Y. Woo (2006)
Development of the transgenic cyan fluorescent protein (CFP)-expressing nude mouse for "Technicolor" cancer imaging.
H. S. Tran Cao (2009)
Chemosensitivity of peritoneal micrometastases as evaluated using a green fluorescence protein (GFP)‐tagged human gastric cancer cell line
H. Nakanishi (2003)
Challenges of stem cell therapy for spinal cord injury: human embryonic stem cells, endogenous neural stem cells, or induced pluripotent stem cells?
M. Ronaghi (2010)
Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections
H. Tahara (1999)
Long-lasting donor passenger leukocytes after hepatic and intestinal transplantation in rats.
S. Inoue (2004)
Structure, dynamics and optical properties of fluorescent proteins: perspectives for marker development.
G. Nienhaus (2009)
This paper is referenced by
Algorithms for improved 3-D reconstruction of live mammalian embryo vasculature from optical coherence tomography data.
Prathamesh M. Kulkarni (2015)
Transgenic nude mice ubiquitously expressing fluorescent proteins for color-coded imaging of the tumor microenvironment.
Robert M Hoffman (2014)
Imaging the Governing Step of Metastasis in Gelfoam® Histoculture.
Robert M Hoffman (2018)
Mammary window chamber model: A platform for multi-modality cancer imaging and dynamic oxygenation assessment
R. Schafer (2015)
Fluorescent angiogenesis models using gelfoam® implanted in transgenic mice expressing fluorescent proteins.
Robert M Hoffman (2014)
S. R. Singh (2014)