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Genetic Engineering Approaches For Algae

S. Rosales-Mendoza
Published 2016 · Biology

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Genetic engineering has allowed for the development of tremendous advances in biomedicine, including the production of biopharmaceuticals (BFs) in several expression hosts; in which algae are considered a next generation platform with attractive attributes. The genetic engineering approaches that allow for the use of transgenic algae as BFs biofactories are analyzed in the present chapter. The transformation methods for addressing chloroplast and nuclear transformation are described including Agrobacterium infection, glass bead treatment, particle bombardment, and electroporation. An updated outlook on the nuclear and chloroplast expression strategies is provided, comparing them in terms of yields, stability, and biosynthetic capacity. Due to recent advances including multicistronic systems, protein secretion approaches, new strains, and selection markers; efficient transgene expression and high protein yields are envisioned. In conclusion current genetic engineering tools are fuelling the developments in the molecular pharming field with relevant prospects for achieving improvements in yields and expanding the spectrum of target species.
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