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The Environment And The Tools In Rhizo- And Bioremediation Of Contaminated Soil

A. K. J. Surridge, F. Wehner, T. E. Cloete
Published 2009 · Environmental Science

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Several methods are available for determining microbial diversity in the soil environment, including different plating methods, light and fluorescence microscopy, and DNA and RNA analysis. Since culturable proportions of bacterial communities from the environment are negligible compared with the number of species that are present in the soil environment, culture techniques for environmental bacterial community diversity analysis are becoming less popular. Molecular techniques have been developed to identify and determine species diversity of microorganisms without isolation. Molecular techniques also provide a rapid means of determining the relative abundance of common species present in a given sample, which do not need to be culturable. Characterization of genes involved in the degradation of organic pollutants has also led to the application of molecular techniques in the microbial ecology of polluted areas.
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