Online citations, reference lists, and bibliographies.
← Back to Search

Antibody Against Single-stranded DNA Detects Both Programmed Cell Death And Drug-induced Apoptosis

I. Naruse, H. Keino, Y. Kawarada
Published 2004 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Cyclophosphamide induced fragmented nuclei in mouse thymic epithelial cells. Agarose gel electrophoresis showed the fragmentation of the DNA extracted from mouse thymus exposed to cyclophosphamide. The cell death induced by cyclophosphamide was considered to be apoptotic. Polyclonal antibody against single-stranded DNA was used immunohistochemically to detect apoptotic cell death in thymic epithelial cells. This antibody also detected programmed cell death in the interdigital necrotic zone of the mouse limb plate on day 14 of gestation, and in the ganglion of the trigeminal nerve on day 13 of gestation. These results show that the antibody specific for single-stranded DNA detected both drug-induced apoptosis and programmed cell death during embryogenesis.
This paper references
Apoptosis. The role of the endonuclease.
M. Arends (1990)
Radiation-induced DNA single-strand breaks in freshly isolated human leukocytes.
J. Buatti (1992)
[Lysosomes of the intestinal epithelium. An embryological investigation].
R. Gossrau (1975)
Apoptosis: a program for death or survival? In: Tomei LD, Cope FD (eds) Apoptosis. The molecular basis of cell death
LD Tomei (1991)
Effects of 5-fluorouracil and cytosine arabinoside on the manifestation of digital malformations in pdn mice.
I. Naruse (1983)
Evidence for the internucleosomal breakage of chromatin in rat thymocytes irradiated in vitro.
T. Yamada (1981)
The fate of DNA originally existing in the zygote nucleus during achromosomal cleavage of fertilized echinoderm eggs in the presence of aphidicolin: Microscopic studies with anti‐DNA antibody
H. Yamada (1985)
Neuronal death in the development of the vertebrate nervous system
P. G. Clarke (1985)
Programmed cell death and apop
RA Lockshin (1991)
Cell death in the morphogenesis and teratogenesis of the heart
Dr. méd. Tomas Pexieder (1975)
Morphogenesis of genetic preaxial polydactyly, polydactyly nagoya, pdn, in mice.
I. Naruse (1982)
Prevention of Genetic Polydactyly in Polydactyly Nagoya (Pdn) Mice in Vitro by Surgical Treatment of Foot Plate during Embryogenesis
I. Naruse (1986)
Cell death in developing systems.
J. Hurlé (1988)
Prevention of polydactyly manifestation in Polydactyly Nagoya (Pdn) mice by administration of cytosine arabinoside during pregnancy.
I. Naruse (1986)
Hereditary and induced modifications of the normal necrotic patterns in the developing limb buds of the rat and mouse: facts and hypotheses
J. Milaire (1983)
Distribution of thymic nurse cells (lymphoepithelial complexes) in the murine thymus
C Hiramine (1991)
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
Y. Gavrieli (1992)
Ectodermal and mesodermal cell death patterns in 6-mercaptopurine riboside-induced digital deformities.
W. J. Scott (1980)
Hydroxyurea induces apoptosis and regular DNA fragmentation in a Burkitt's lymphoma cell line.
C. Johnson (1992)
The Cell Cycle and Cell Population Kinetics in the Programmed Cell Death in the Limb‐Buds of Normal and 5‐Bromodeoxyuridine‐ Treated Chick Embryos
S. Tóne (1988)
Distribution of thymic nurse cells (lymphoepithelial complexes) in the murine thymus
C Hiramine (1991)
A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA.
J. Wijsman (1993)
Programmed cell death and apoptosis
Ra eds Lockshin (1991)
2,3,7,8-Tetrachlorodibenzo-p-dioxin kills immature thymocytes by Ca2+-mediated endonuclease activation.
D. McConkey (1988)
Anti-poly(L).poly(C) antibody bound to cellulose and its use in the specific separation of double-stranded RNAs.
Y. Kitagawa (1981)
Morphogenesis of genetic preaxiaI polydactyly
I Naruse (1982)
Apoptosis: The Molecular Basis of Cell Death
L. D. Tomei (1991)
Molecular cytological study of programmed cell death in chick limb-buds
S. Tone (1989)
Delayed appearance of ectodermal cell death as a mechanism of polydactyly induction
W. Scott (1977)
Death in Embryonic Systems
J. W. Saunders (1966)
Programmed cell death during Drosophila embryogenesis.
J. M. Abrams (1993)
droxyurea induces apoptosis and regular fragmentation in a Burkitt ' s lymphoma cell line
CA Johnson (1992)

This paper is referenced by
Formation of 8-hydroxydeoxyguanosine and cell-cycle arrest in the rat liver via generation of oxidative stress by phenobarbital: association with expression profiles of p21(WAF1/Cip1), cyclin D1 and Ogg1.
A. Kinoshita (2002)
Serum Thymic Factor Prevents LPS‐Induced Pancreatic Cell Damage in Mice via Up‐Regulation of Bcl‐2 Expression in Pancreas
N. Saitoh (2004)
Postnatal apoptosis, development, and sex difference in the lateral septum of rats
S. Tsukahara (2004)
Function of Draxin on Hippocampal Development
Zhang Sanbing (2016)
Loss and apoptosis of smooth muscle cells in intracranial aneurysms studies with in situ DNA end labeling and antibody against single-stranded DNA
T. Sakaki (2005)
Induction of Apoptosis in Leukemic Cells by Homovanillic Acid Derivative, Capsaicin, through Oxidative Stress
K. Ito (2004)
Radiofrequenzablation als „minimal-invasive Technik“ zur Tumorbehandlung?
T. Schneider (2008)
Draxin is involved in the proper development of the dI3 interneuron in chick spinal cord
Yuhong Su (2010)
The three cases of hepatocellular carcinoma treated by surgical operation after
H. Nakamura (2009)
Carboplatin Induces Less Apoptosis in the Cochlea of Guinea Pigs than Cisplatin
K. Watanabe (2002)
Cytoplasmic Expression and Extracellular Deposition of an Antiangiogenic Factor, Pigment Epithelium-Derived Factor, in Human Atherosclerotic Plaques
H. Baba (2005)
Six1 and Six4 promote survival of sensory neurons during early trigeminal gangliogenesis
Y. Konishi (2006)
Homeostatic Mass Control in Gastric Non-Neoplastic Epithelia under Infection of Helicobacter pylori: An Immunohistochemical Analysis of Cell Growth, Stem Cells and Programmed Cell Death
K. Kato (2008)
Effects of repeated maternal stress on FOS expression in the hypothalamic paraventricular nucleus of fetal rats
I. Tobe (2005)
Effects of ryanodine receptor activation on neurotransmitter release and neuronal cell death following kainic acid-induced status epilepticus
F. Mori (2005)
Mechanistic approach of contrasting modifying effects of caffeine on carcinogenesis in the rat colon and mammary gland induced with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine.
F. Takeshita (2003)
In situ observation of germinal center cell apoptosis during a secondary immune response.
H. Saitoh (2006)
Modern Research Techniques of Apoptotic Cell Death (Review)
Pozharsky Square (2015)
Role of p38 MAP kinase pathway in a toxin-induced model of hemolytic uremic syndrome
X. Fu (2004)
Method of Specific Detection of Apoptosis Using Formamide-induced DNA Denaturation Assay
Y. Ito (2006)
8-Oxoguanine causes neurodegeneration during MUTYH-mediated DNA base excision repair.
Zijing Sheng (2012)
Proliferation and apoptosis in the developing human neocortex
W. Chan (2002)
Replication gaps are a cancer vulnerability counteracted by translesion synthesis
Sumeet Nayak (2019)
[Radiofrequency ablation as minimally invasive treatment for tumors : a commentary on Schultheis et al].
T. Schneider (2008)
Expression of single-stranded DNA and p53 in delayed neuronal death after transient ischemia in the gerbil hippocampus
M. Maeda (2013)
Increased numbers of oligodendrocyte lineage cells in the optic nerves of cerebroside sulfotransferase knockout mice
Hitoshi Kajigaya (2011)
Reactive Oxygen Species Implication of Phosphorylation of p 53 at Ser-15 Residue by Acid Derivative , Capsaicin , through Oxidative Stress : Induction of Apoptosis in Leukemic Cells by Homovanillic
K. Ito (2004)
Treatment with pravastatin attenuates progression of chronic pancreatitis in rat
L. Wei (2011)
Thiamine deficiency induces massive cell death in the olfactory bulbs of mice.
Shun Hamada (2013)
Hydrogen peroxide-induced cell death in normal human keratinocytes is differentiation dependent.
Thomas Zuliani (2005)
p51/p63, a novel p53 homologue, potentiates p53 activity and is a human cancer gene therapy candidate
R. Kunisaki (2006)
Involvement of postnatal apoptosis on sex difference in number of cells generated during late fetal period in the sexually dimorphic nucleus of the preoptic area in rats
Y. Kato (2012)
See more
Semantic Scholar Logo Some data provided by SemanticScholar