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Rise In Chlorotetracycline Fluorescence Accompanies Tracheary Element Differentiation In Suspension Cultures OfZinnia

A. W. Roberts, C. Haigler
Published 2005 · Biology

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SummaryDeveloping tracheary elements in suspension cultures ofZinnia elegans fluoresce intensely relative to non-differentiating cells when stained with chlorotetracycline (CTC), a fluorescent chelate probe for membrane associated calcium. This suggests that a change in calcium uptake or subcellular distribution accompanies the onset of tracheary element differentiation. A few cells in early differentiating cultures were brightly fluorescent, but did not have visible cell wall thickenings, suggesting that a rise in sequestered calcium may precede visible differentiation. Diffuse CTC fluorescence in early differentiation most likely results from sequestration of calcium in the endoplasmic reticulum. Late in differentiation, CTC fluorescence becomes punctate in appearance, probably due to loss of plasma membrane integrity occurring at the onset of autolysis.Zinnia suspension culture cells were found to be very sensitive to CTC and low concentrations (10 μM) were used to assure accurate localization of membrane-associated calcium in healthy cells.
This paper references
10.1007/BF00385156
Localization of membrane-associated calcium following cytokinin treatment in Funaria using chlorotetracycline
M. J. Saunders (2004)
10.1002/CM.970100120
Dynamics of the endoplasmic reticulum in living onion epidermal cells in relation to microtubules, microfilaments, and intracellular particle movement
N. Allen (1988)
10.1177/35.9.3611737
Ultrastructural localization of intracellular calcium stores by a new cytochemical method.
M. Poenie (1987)
Structural aspects of tracheary element differentiation in suspension cultures of Zinnia elegans
AW Roberts (1989)
10.1146/ANNUREV.PP.36.060185.002145
CALCIUM AND PLANT DEVELOPMENT
P. Hepler (1985)
10.1007/BF01282956
Interrelation between the spatial disposition of actin filaments and microtubules during the differentiation of tracheary elements in culturedZinnia cells
H. Kobayashi (2005)
The role of calcium in metabolic control. In: Davies DD (ed) The biochemistry of plants, vo112
EF Allan (1987)
Interpolation of microtubules into cortical arrays during cell elongation and differentiation in roots of Azolla pinnata.
A. Hardham (1979)
10.1007/BF01281541
Local accumulation of membrane-associated calcium according to cell pattern formation inMicrasterias denticulata, visualized by chlorotetracycline fluorescence
U. Meindl (2005)
10.1126/science.1198106
Calcium ion distribution in cytoplasm visualised by aequorin: diffusion in cytosol restricted by energized sequestering
B. Rose (1975)
10.1007/BF01870574
Effects of divalent cations, temperature, osmotic pressure gradient, and vesicle curvature on phosphatidylserine vesicle fusion
S. Ohki (2005)
10.1016/0012-1606(84)90125-8
The endoplasmic reticulum in the cortex of developing guard cells: coordinate studies with chlorotetracycline and osmium ferricyanide.
B. A. Palevitz (1984)
10.1104/PP.65.1.57
Establishment of an Experimental System for the Study of Tracheary Element Differentiation from Single Cells Isolated from the Mesophyll of Zinnia elegans.
H. Fukuda (1980)
Microtubule assembly-disassembly: effects of calcium ion and calmodulin
GC Na (1988)
10.1016/0098-8472(87)90038-4
Evidence that auxin-induced xylogenesis in Lactuca explants requires calmodulin
L. W. Roberts (1987)
The relationship between cell organelles and cell wall thickenings in primary tracheary elements of the cucumber . I . Morphological aspects
CH Haigler (1986)
Effects of divalent cations, temperature, osmotic
S Ohki (1984)
10.1002/9780470513347.CH14
Calcium and the regulation of cytoskeletal assembly, structure and contractility.
M. Mooseker (1986)
10.1016/0006-291X(71)90359-7
Visualization of membrane bound cations by a fluorescent technique.
A. Caswell (1971)
10.1007/BF01867846
Tetracycline fluorescence as calcium-probe for nerve membrane with some model studies using erythrocyte ghosts
M. Hallett (2005)
10.1080/07352688709382247
Calcium messenger system in plants.
B. W. Poovaiah (1987)
10.1016/0079-6107(82)90011-6
Measurement of Ca2+ concentrations in living cells.
J. R. Blinks (1982)
10.1007/BF01282989
Endoplasmic reticulum and cytoplasmic streaming: Fluorescence microscopical observations in adaxial epidermis cells of onion bulb scales
H. Quader (2005)
10.1093/OXFORDJOURNALS.PCP.A077494
Secondary Cell Wall Formation: Changes in Cell Wall Constituents during the Differentiation of Isolated Mesophyll Cells of Zinnia elegans to Tracheary Elements
Edgar Ingold (1988)
Visualization of Ca2+-gradients in germinating aplanospores of Vaucheria longicaulis var. macounii Blum (Tribophyceae) with chlorotetracycline fluorescence
L. Oliveira (1988)
10.1104/PP.88.1.92
Kinetics of determination in the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elements.
D. Church (1988)
10.1016/0739-6260(84)90046-7
Comparison of tracheary element differentiation in intact leaves and isolated mesophyll cells of zinnia elegans
J. Burgess (1984)
Biochemie der Cellulose-Biosynthese
D. Delmer (1987)
10.1007/BF00391091
Localization of membrane-associated calcium during development of fucoid algae using chlorotetracycline
D. Kropf (2004)
10.1007/BF01276294
Visualization of the Ca2+-gradient in growing pollen tubes ofLilium longiflorum with chlorotetracycline fluorescence
H. Reiss (2005)
10.1083/JCB.87.1.23
Detection of the membrane-calcium distribution during mitosis in Haemanthus endosperm with chlorotetracycline
S. M. Wolniak (1980)
10.1007/BF00351376
Calcium promoted phenolic coupling by superoxide radical —a possible lignification reaction in wood
U. Westermark (2004)
10.1093/JXB/22.3.756
The Use of Non-permeating Pigments for Testing the Survival of Cells
D. Gaff (1971)
10.1007/BF01746608
Membrane-bound Ca2+ distribution visualized by chlorotetracycline fluorescence during morphogenesis of soredia in a lichen
M. Raineri (2005)
10.1038/325279A0
Regulation of cellulose synthesis in Acetobacter xylinum by cyclic diguanylic acid
P. Ross (1987)
10.1111/J.1438-8677.1973.TB00849.X
THE RELATIONSHIP BETWEEN CELL ORGANELLES AND CELL WALL THICKENINGS IN PRIMARY TRACHEARY ELEMENTS OF THE CUCUMBER II. QUANTITATIVE ASPECTS
Leentje Goosen-De Roo (1973)
10.1007/BF01281826
Membrane-associated calcium during pollen grain germination: a microfluorometric analysis
V. Polito (2005)
10.1007/BF01275709
Transport of rosettes from the golgi apparatus to the plasma membrane in isolated mesophyll cells ofZinnia elegans during differentiation to tracheary elements in suspension culture
C. Haigler (2005)
10.1083/JCB.86.2.500
Localization of Ca++-containing antimonate precipitates during mitosis
S. M. Wick (1980)
10.1083/JCB.88.3.526
Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy
L. Johnson (1981)
10.1007/BF01279806
The tip-to-base calcium gradient in pollen tubes ofLilium longiflorum measured by proton-induced X-ray emission (PIXE)
H. Reiss (2005)
The role of calcium in metabolic control
EF Allan (1987)
10.1093/OXFORDJOURNALS.PCP.A076556
Changes in the Synthesis of RNA and Protein during Tracheary Element Differentiation in Single Cells Isolated from the Mesophyll of Zinnia elegans
H. Fukuda (1983)
10.1007/978-94-009-3585-3_19
The Induction of Vascular Tissues by Auxin
R. Aloni (1987)
10.1083/JCB.76.2.386
Intracellular divalent cation release in pancreatic acinar cells during stimulus-secretion coupling. II. Subcellular localization of the fluorescent probe chlorotetracycline
D. Chandler (1978)
10.1007/BF00384638
Incorporation of radioactivity into wheat xylem walls
J. Pickett-Heaps (2004)
10.1007/BF00388841
Calcium gradients in tip growing plant cells visualized by chlorotetracycline fluorescence
H. D. Reiss (2004)



This paper is referenced by
10.1007/BF01280301
Plasma membrane-cell wall connections: Roles in mitosis and cytokinesis revealed by plasmolysis ofTradescantia virginiana leaf epidermal cells
A. Cleary (2005)
10.1023/A:1001884131184
Secondary wall deposition in tracheary elements of cucumber grown in vitro
S. Kaliamoorthy (2004)
10.1016/S0070-2153(05)71007-3
Cell death and organ development in plants.
H. Rogers (2005)
10.1007/BF02411447
Tracheary-element differentiation in suspension-cultured cells ofZinnia requires uptake of extracellular Ca2+
A. W. Roberts (1990)
10.1007/S11515-006-0026-7
Ultracytochemical localization of Ca2+ during the phloem ganglion development in Phyllostachys edulis
Yu Fen (2006)
10.1007/s00709-004-0064-4
Roles of microtubules and cellulose microfibril assembly in the localization of secondary-cell-wall deposition in developing tracheary elements
A. W. Roberts (2004)
10.1023/A:1026532223173
Programmed cell death of tracheary elements as a paradigm in plants
H. Fukuda (2004)
10.1007/978-3-319-21033-9_3
Life Beyond Death: The Formation of Xylem Sap Conduits
Delphine Ménard (2015)
10.1007/s00425-007-0660-6
Oligogalacturonides enhance cytokinin-induced vegetative shoot formation in tobacco explants, inhibit polyamine biosynthetic gene expression, and promote long-term remobilisation of cell calcium
G. Falasca (2007)
10.1007/BF01280293
Microtubule rearrangements accompanying dedifferentiation in mesophyll cultures ofZinnia elegans L.
Kenneth S. Uhnak (2005)
10.1007/BF00189814
Changes in calcium and calmodulin levels during microsporogenesis, pollen development and germination in Gasteria verrucosa (Mill.) H. Duval
U. Tirlapur (2004)
10.1007/BF01882027
Cortical actin filaments fragment and aggregate to form chloroplast-associated and free F-actin rings in mechanically isolatedZinnia mesophyll cells
A. O. Frost (2005)
10.1104/pp.105.066464
Lectin Receptor Kinases Participate in Protein-Protein Interactions to Mediate Plasma Membrane-Cell Wall Adhesions in Arabidopsis1
A. Gouget (2005)
10.1093/mp/ssp045
Analysis of transcriptome changes induced by Ptr ToxA in wheat provides insights into the mechanisms of plant susceptibility.
I. Pandelova (2009)
10.1007/BF01281321
Comparing plant and animal extracellular matrix-cytoskeleton connections — are they alike?
C. Reuzeau (2005)
10.1111/J.1365-313X.2006.02950.X
Arabidopsis hot2 encodes an endochitinase-like protein that is essential for tolerance to heat, salt and drought stresses.
Yerim Kwon (2007)
10.1007/BF00027197
Tracheary element differentiation in Zinnia mesophyll cell cultures
D. Church (2004)
10.1007/BF00197540
Involvement of calmodulin and calmodulin-binding proteins in the differentiation of tracheary elements in Zinnia cells
H. Kobayashi (2004)
10.1007/s11515-006-0026-7
Ultracytochemical localization of Ca2+ during the phloem ganglion development in Phyllostachys edulis
Fen Yu (2006)
10.1002/cyto.a.20194
Flow cytometric analysis of tracheary element differentiation in Zinnia elegans cells
I. Weir (2005)
10.3390/plants9030373
Effects of Rhizome Integration on the Water Physiology of Phyllostachys edulis Clones under Heterogeneous Water Stress
Xiong Jing (2020)
10.1007/BF00198040
Methylxanthines reversibly inhibit tracheary-element differentiation in suspension cultures of Zinnia elegans L.
A. W. Roberts (2004)
10.1007/BF00039912
A simplified medium for in vitro tracheary element differentiation in mesophyll suspension cultures from Zinnia elegans L.
A. W. Roberts (2004)
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