Online citations, reference lists, and bibliographies.
← Back to Search

Array Biosensor For Detection Of Toxins

F. Ligler, C. Taitt, L. Shriver-Lake, K. Sapsford, Y. Shubin, J. Golden
Published 2003 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The array biosensor is capable of detecting multiple targets rapidly and simultaneously on the surface of a single waveguide. Sandwich and competitive fluoroimmunoassays have been developed to detect high and low molecular weight toxins, respectively, in complex samples. Recognition molecules (usually antibodies) were first immobilized in specific locations on the waveguide and the resultant patterned array was used to interrogate up to 12 different samples for the presence of multiple different analytes. Upon binding of a fluorescent analyte or fluorescent immunocomplex, the pattern of fluorescent spots was detected using a CCD camera. Automated image analysis was used to determine a mean fluorescence value for each assay spot and to subtract the local background signal. The location of the spot and its mean fluorescence value were used to determine the toxin identity and concentration. Toxins were measured in clinical fluids, environmental samples and foods, with minimal sample preparation. Results are shown for rapid analyses of staphylococcal enterotoxin B, ricin, cholera toxin, botulinum toxoids, trinitrotoluene, and the mycotoxin fumonisin. Toxins were detected at levels as low as 0.5 ng mL−1.
This paper references
10.1016/S0956-5663(00)00055-5
Improved multianalyte detection of organophosphates and carbamates with disposable multielectrode biosensors using recombinant mutants of Drosophila acetylcholinesterase and artificial neural networks.
T. Bachmann (2000)
10.1016/0003-2697(89)90607-6
Acetylcholine receptor fiber-optic evanescent fluorosensor.
K. R. Rogers (1989)
10.1021/AC960071+
Fiber-optic evanescent wave biosensor for the detection of oligonucleotides.
A. Abel (1996)
[Prevalence of Staphylococcus aureus among food handlers from a metropolitan university in Chile].
A. Soto (1996)
10.1021/AC000846L
Gangliosides as receptors for biological toxins: development of sensitive fluoroimmunoassays using ganglioside-bearing liposomes.
A. Singh (2000)
10.1007/BF02783583
Detection of endotoxin using an evanescent wave fiber-optic biosensor
E. James (1996)
10.1016/0272-0590(91)90166-2
Acetylcholinesterase fiber-optic biosensor for detection of anticholinesterases.
K. Rogers (1991)
10.1021/AC990183B
Multiple-analyte fluoroimmunoassay using an integrated optical waveguide sensor.
T. E. Plowman (1999)
10.1021/AC980798T
An Array Immunosensor for Simultaneous Detection of Clinical Analytes
Chris A. Rowe (1999)
10.1016/S0167-7799(01)01690-0
Detection of physiologically active compounds using cell-based biosensors.
D. Stenger (2001)
10.1016/S0956-5663(99)00052-4
Array biosensor for detection of biohazards.
C. A. Rowe-Taitt (2000)
10.1006/ABIO.1996.0006
Quantitating staphylococcal enterotoxin B in diverse media using a portable fiber-optic biosensor.
L. Tempelman (1996)
10.1016/S0956-5663(99)00043-3
Comparison of chemical cleaning methods of glass in preparation for silanization
J. J. Cras (1999)
10.1128/IAI.64.11.4567-4573.1996
Rapid clearance of the bacterial superantigen staphylococcal enterotoxin B in vivo.
R. Vabulas (1996)
10.1016/S0956-5663(97)00027-4
Fiber optic-based biosensor for ricin.
U. Narang (1997)
10.1099/00221287-76-2-417
Subunit structure of cholera toxin.
I. Loennroth (1973)
10.1016/S0956-5663(97)00113-9
Detection of multiple toxic agents using a planar array immunosensor.
R. M. Wadkins (1998)
10.1021/BI00597A024
Interaction of choleragen with the oligosaccharide of ganglioside GM1: evidence for multiple oligosaccharide binding sites.
P. H. Fishman (1978)
10.1016/S0956-5663(00)00105-6
Structure and activity of lipid membrane biosensor surfaces studied with atomic force microscopy and a resonant mirror.
M. I. Fisher (2000)
10.1016/0009-8981(90)90305-C
Fluorescence spectroscopy and its application to a new generation of high sensitivity, multi-microspot, multianalyte, immunoassay.
R. Ekins (1990)
10.1016/S0003-2670(98)00086-5
Optical immunoprobe development for multiresidue monitoring in water
A. Brecht (1998)
10.1093/clinchem/44.9.2036
Mass-sensing, multianalyte microarray immunoassay with imaging detection.
J. W. Silzel (1998)
10.1021/AC010542E
Electrochemical and quartz crystal microbalance detection of the cholera toxin employing horseradish peroxidase and GM1-functionalized liposomes.
L. Alfonta (2001)
10.1021/AC981425V
Array biosensor for simultaneous identification of bacterial, viral, and protein analytes.
C. Rowe (1999)
10.1021/AC990406Y
Amperometric detection of Escherichia coli heat-labile enterotoxin by redox diacetylenic vesicles on a sol-gel thin-film electrode.
T. Peng (2000)
10.1021/AC015554E
Kinetics of antigen binding to arrays of antibodies in different sized spots.
K. Sapsford (2001)
10.1016/0003-2697(92)90215-S
Detection of nicotinic receptor ligands with a light addressable potentiometric sensor.
K. R. Rogers (1992)
10.1099/00222615-28-3-163
Enterotoxin production by Staphylococcus aureus isolates from cases of septicaemia and from healthy carriers.
H. Humphreys (1989)
10.1021/AC0157268
Demonstration of four immunoassay formats using the array biosensor.
K. Sapsford (2002)
10.1016/S0956-5663(99)00053-6
Multi-analyte interrogation using the fiber optic biosensor.
G. P. Anderson (2000)
10.1006/ABIO.2001.5024
Detection of multivalent interactions through two-tiered energy transfer.
X. Song (2001)
10.1006/ABIO.2000.4571
A ganglioside-based assay for cholera toxin using an array biosensor.
C. A. Rowe-Taitt (2000)
10.1021/AC981145F
Direct, ultrasensitive, and selective optical detection of protein toxins using multivalent interactions.
X. Song (1999)
10.1021/AC970929A
Surface Plasmon Resonance Multisensing
C. Berger (1998)
10.1007/S00216-001-1127-4
A disposable acetylcholinesterase-based electrode biosensor to detect anatoxin-a(s) in water
F. Villatte (2002)
10.1002/(SICI)1520-6521(1998)2:6<341::AID-FACT4>3.0.CO;2-X
Field demonstration of a portable flow immunosensor
A. Kusterbeck (1998)
10.1021/AC0260185
Nine-analyte detection using an array-based biosensor.
C. R. Taitt (2002)
10.1007/s002490100133
Bioadhesion of supramolecular structures at supported planar bilayers as studied by the quartz crystal microbalance
M. Liebau (2001)
10.1016/S0176-6724(88)80146-9
Prevalence of serum antibodies to toxic-shock-syndrome-toxin-1 and to staphylococcal enterotoxins A, B and C in West-Germany.
E. Schroeder (1988)
10.1016/S0956-5663(00)00122-6
Simultaneous detection of six biohazardous agents using a planar waveguide array biosensor.
C. A. Rowe-Taitt (2000)
10.1128/AEM.68.8.4102-4106.2002
Detection of Anatoxin-a(s) in Environmental Samples of Cyanobacteria by Using a Biosensor with Engineered Acetylcholinesterases
E. Devic (2002)
10.1023/A:1009900608757
Array Biosensor: Optical and Fluidics Systems
M. Feldstein (1999)
10.1002/(SICI)1520-6521(1999)3:4/5<307::AID-FACT9>3.0.CO;2-M
Biological agent detection with the use of an airborne biosensor
G. M. Anderson (1999)
10.1021/CM990726Y
Sol−Gel Encapsulated Anti-Trinitrotoluene Antibodies in Immunoassays for TNT
E. Lan (2000)
10.1021/LA971231V
Step-and-repeat photopatterning of protein features using caged-biotin-BSA: Characterization and resolution
A. S. Blawas (1998)



This paper is referenced by
10.1080/01480540701375232
Mycotoxin Assays Using Biosensor Technology: A Review
M. Pohanka (2007)
10.1002/ADFM.200901232
Highly Stable Au Nanoparticles with Tunable Spacing and Their Potential Application in Surface Plasmon Resonance Biosensors
Shuyan Gao (2010)
10.3390/s121114556
Catalytic and Inhibitory Kinetic Behavior of Horseradish Peroxidase on the Electrode Surface
Jitao Huang (2012)
10.1134/S1068162009030078
Preparation and characterization of monoclonal antibodies to the cholera toxin
E. E. Petrova (2009)
10.1039/c4an00345d
Rapid and simultaneous detection of ricin, staphylococcal enterotoxin B and saxitoxin by chemiluminescence-based microarray immunoassay.
A. Szkola (2014)
10.1016/j.talanta.2005.06.039
Towards advanced chemical and biological nanosensors-An overview.
C. R. Yonzon (2005)
10.1016/J.AB.2006.05.003
Detection of ricin and other ribosome-inactivating proteins by an immuno-polymerase chain reaction assay.
C. Lubelli (2006)
10.1007/S11214-007-9276-1
Protein Microarrays-Based Strategies for Life Detection in Astrobiology
V. Parro (2008)
10.1080/03067310903029822
Detection of ricin toxin in water using immunoassays
Junqi Yue (2009)
10.1039/b911525k
Simultaneous quantification of five bacterial and plant toxins from complex matrices using a multiplexed fluorescent magnetic suspension assay.
D. Pauly (2009)
10.1007/s00216-019-01808-z
Magnetic nanocomposites: versatile tool for the combination of immunomagnetic separation with flow-based chemiluminescence immunochip for rapid biosensing of Staphylococcal enterotoxin B in milk
Angelika Nistler (2019)
10.1007/1-4020-3384-2_2
NATURAL TOXINS: THE PAST AND THE PRESENT
E. Grishin (2005)
10.1016/J.SNB.2010.02.009
Multi-wavelength Spatial LED illumination based detector for in vitro detection of Botulinum Neurotoxin A Activity.
S. Sun (2010)
10.1016/J.SNB.2013.06.030
Capillary Array Waveguide Amplified Fluorescence Detector for mHealth.
Joshua Balsam (2013)
Optimisation de nanostructures plasmoniques pour la détection et la caractérisation structurelle des protéines par Diffusion Raman Exaltée de Surface
Maximilien Cottat (2014)
10.1063/1.4800933
Nanostructured magnesium oxide biosensing platform for cholera detection
Manoj Kumar Patel (2013)
10.1134/S1061934817040025
Application of the localized surface plasmon resonance of gold nanoparticles for the determination of 1,1-dimethylhydrazine in water: Toward green analytical chemistry
Ali Reza Zarei (2017)
10.6000/1927-5129.2013.09.72
Editorial: Botulinum Neurotoxin: Advances in Diagnostics and Healthcare Applications
Sandeep Kumar Vashist (2013)
10.1002/SAM.V3:5
A machine-learning approach to detecting unknown bacterial serovars
Ferit Akova (2010)
10.1089/nat.2012.0377
Nucleic acid aptamers against biotoxins: a new paradigm toward the treatment and diagnostic approach.
L. Lauridsen (2012)
10.1039/c3an02032k
Multiplex chemiluminescent immunoassay for screening of mycotoxins using photonic crystal microsphere suspension array.
Kun Xu (2014)
10.1016/J.BIOS.2006.01.003
Development of biosensors for cancer clinical testing.
A. Rasooly (2006)
Studies of Ligand-Receptor Pairs Utilizing Polymerized Planar Supported Lipid Bilayers
B. Liang (2013)
10.1016/j.bios.2011.07.046
P450-based porous silicon biosensor for arachidonic acid detection.
A. Giovannozzi (2011)
10.1007/s12550-009-0028-9
An antibody-based microarray assay for the simultaneous detection of aflatoxin B1 and fumonisin B1
Ilaria Lamberti (2009)
10.3390/toxins2010024
Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection
P. Čapek (2010)
10.1109/JSEN.2008.926964
Optical PMMA Chip Suitable for Multianalyte Detection
F. Baldini (2008)
10.1109/ICSENS.2010.5690201
Label-free immunosensor using a gold electrode covered with conductive self-assembled monolayer
Kimiyasu Takoh (2010)
10.4025/actasciagron.v34i4.11284
Development of a bioassay to quantify the ricin toxin content of castor bean ( Ricinus communis L.) seeds - doi: 10.4025/actasciagron.v34i4.11284
Carlos Alberto Rauer Demant (2011)
10.1016/B978-044452784-4/50004-6
3 – Fractal Analysis of Toxin and Pathogen Detection on Biosensor Surfaces
A. Sadana (2006)
10.1201/9781420028287.CH12
Planar Waveguide Biosensors for Point-of-Care Clinical and Molecular Diagnostics
D. A. Christensen (2005)
10.1016/J.JIM.2005.04.003
Comparison of electrochemiluminescence assay and ELISA for the detection of Clostridium botulinum type B neurotoxin.
V. Guglielmo-Viret (2005)
See more
Semantic Scholar Logo Some data provided by SemanticScholar