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Removal Of Toxic Chromate Using Free And Immobilized Cr(VI)-reducing Bacterial Cells Of Intrasporangium Sp. Q5-1
J. Yang, Minyan He, G. Wang
Published 2009 · Biology
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Chromate-reducing microorganisms with the ability of reducing toxic chromate [Cr(VI)] into insoluble trivalent chromium [Cr(III)] are very useful in treatment of Cr(VI)-contaminated water. In this study, a novel chromate-reducing bacterium was isolated from Mn/Cr-contaminated soil. Based on morphological, physiological/biochemical characteristics and 16S rRNA gene sequence analyses, this strain was identified as Intrasporangium sp. strain Q5-1. This bacterium has high Cr(VI) resistance with a MIC of 17 mmol l−1 and is able to reduce Cr(VI) aerobically. The best condition of Cr(VI) reduction for Q5-1 is pH 8.0 at 37°C. Strain Q5-1 is also able to reduce Cr(VI) in resting (non-growth) conditions using a variety of carbon sources as well as in the absence of a carbon source. Acetate (1 mmol l−1) is the most efficient carbon source for stimulating Cr(VI) reduction. In order to apply strain Q5-1 to remove Cr(VI) from wastewater, the bacterial cells were immobilized with different matrices. Q5-1 cells embedded with compounding beads containing 4% PVA, 3% sodium alginate, 1.5% active carbon and 3% diatomite showed a similar Cr(VI) reduction rates to that of free cells. In addition, the immobilized Q5-1 cells have the advantages over free cells in being more stable, easier to re-use and minimal clogging in continuous systems. This study provides potential applications of a novel immobilized chromate-reducing bacterium for Cr(VI) bioremediation.
This paper references
Hexavalent chromate reduction by immobilized Streptomyces griseus
Ashwini C. Poopal (2008)
Toxic and mutagenic effects of chromium(VI). A review
M. Cieślak-Golonka (1996)
Novel hybrid immobilization of microorganisms and its applications to biological denitrification
S. H. Song (2005)
Molecular cloning: a laboratory manual 3
J. Sambrook (2001)
Aerobic Cr(VI) reduction by Thermus scotoductus strain SA‐01
D. Opperman (2007)
Reduction and efflux of chromate
C Cervantes (2007)
Chromate reductase activity of Enterobacter aerogenes is induced by nitrite.
D. Clark (1994)
A New Genus of the Actinomycetales—Intrasporangium gen.nov.
L. V. Kalakoutskii (1967)
MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment
S. Kumar (2004)
Molecular microbiology of heavy metals
D. Nies (2007)
A method for removal of toxic chromium using dialysis-sac cultures of a chromate-reducing strain of Enterobacter cloacae
K. Komori (2004)
Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate.
F. A. O. Camargo (2003)
Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456.
Woo-Chul Bae (2005)
Janibacter anophelis sp. nov., isolated from the midgut of Anopheles arabiensis.
P. Kämpfer (2006)
In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2+
F. Camargo (2003)
Toxicity of Hexavalent Chromium and Its Reduction by Bacteria Isolated from Soil Contaminated with Tannery Waste
M. Megharaj (2003)
The role of microorganisms in biosorption of toxic metals and radionuclides
C. White (1995)
Reduction and Efflux of Chromate by Bacteria
C. Cervantes (2007)
The situation and improved
Y Guo (2006)
Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate
J. McLean (2001)
Standard Methods for the Examination of Water and Wastewater seventh edition
A. E. Greenberg (2013)
Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction.
K. Wilson (1990)
Bioremediation of toxic chromium from electroplating effluent by chromate-reducing Pseudomonas aeruginosa A2Chr in two bioreactors
A. Ganguli (2001)
Toxicity and Carcinogenicity of Chromium Compounds in Humans
M. Costa (2006)
Bioremediation of chromium contaminated soil: optimization of operating parameters under laboratory conditions.
J. Jeyasingh (2005)
Chromate Reduction in Shewanellaoneidensis MR‐1 Is an Inducible Process Associated with Anaerobic Growth
S. Viamajala (2002)
Enzymatic Reduction of Hexavalent Chromium by Hexavalent Chromium Tolerant Pseudomonas ambigua G-1
H. Horitsu (1987)
Studies on genotoxicity
CT Dillon (2001)
Studies on the genotoxicity of chromium: from the test tube to the cell
R. Codd (2001)
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
J. Thompson (1997)
Comparison of in vitro Cr(VI) reduction by CFEs of chromate resistant bacteria isolated from chromate contaminated soil.
A. Sarangi (2008)
Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR‐1
C. Myers (2000)
Reduction of Cr(VI) by a Bacillus sp.
R. Elangovan (2005)
Evaluation of in vitro
C Desai (2008)
Chromate reduction and 16S rRNA identification of bacteria isolated from a Cr(VI)-contaminated site
P. Pattanapipitpaisal (2001)
Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals.
S. Sultan (2007)
Evaluation of in vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill.
Chirayu Desai (2008)
Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase
C. H. Park (2000)
Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain
J. Campos (2004)
Bioremediation of hexavalent chromium in soil microcosms
P. Salunkhe (2004)
Bergey's Manual of Determinative Bacteriology.
R. E. Buchanan (1975)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Toxic and mutagenic effects of Cr ( VI ) — a review
M Cieslak-Golonka (1995)
Factors affecting hexavalent chromium reduction in pure cultures of bacteria
Yi-tin Wang (1995)
Reduction of Cr(VI) by immobilized cells of Desulfovibrio vulgaris NCIMB 8303 and Microbacterium sp. NCIMB 13776.
A. Humphries (2005)
Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics.
G. Puzon (2005)
Effect of chromate stress on Escherichia coli K-12.
D. Ackerley (2006)
Diagnostic microbiology, 2nd edn
RW Bailey (1966)
Gut microflora & toxic metals: chromium as a model.
R. K. Upreti (2004)
Cr(VI) reduction by Bacillus sp. isolated from chromium landfill
Y. Liu (2006)
This paper is referenced by
Sodium Alginate/Polyvinyl Alcohol Immobilization of Brevibacillus brevis OZF6 Isolated from Waste Water and Its Role in the Removal of Toxic Chromate
P. A. Wani (2016)
Recognition of a New Cr(VI)-Reducing Strain and Study of the Potential Capacity for Reduction of Cr(VI) of the Strain
Chunyong Wang (2019)
Development of biointegrated electrospun nanofibers for environmental applications
Ömer Faruk Sarıoğlu (2016)
Potential Microbial Diversity in Chromium Mining Areas: A Review
Alolika Basu (2015)
Phosphate solubilization and chromium (VI) remediation potential of Klebsiella sp. strain CPSB4 isolated from the chromium contaminated agricultural soil.
Pratishtha Gupta (2018)
In situ vadose zone bioremediation.
P. Höhener (2014)
A Bacillus subtilis strain can reduce hexavalent chromium to trivalent and an nfrA gene is involved
Z. Zheng (2015)
Tratamento de águas contaminadas com crómio(VI) por biossorção em algas marinhas
Frederico Duarte de Sousa Pina (2011)
PRODUCCIÓN LIMPIA Y BIORREMEDIACIÓN PARA DISMINUCIÓN DE LA CONTAMINACIÓN POR CROMO EN LA INDUSTRIA DE CURTIEMBRES
Neyla Benítez-Campo (2011)
Intrasporangium chromatireducens sp. nov., a chromate-reducing actinobacterium isolated from manganese mining soil, and emended description of the genus Intrasporangium.
H. Liu (2012)
Reduction of Hexavalent Chromium by Immobilized Viable Cells of Arthrobacter sp. SUK 1201
Satarupa Dey (2014)
Research progress in microbial chromium-transformation and resistance and bioremediation
Xia Xian (2017)
Isolating, screening and applying chromium reducing bacteria to promote growth and yield of okra (Hibiscus esculentus L.) in chromium contaminated soils.
Zahid Maqbool (2015)
Bioremediation of Hexavalent Chromium by Chromium Resistant Bacteria Reduces Phytotoxicity
Shanewaz Hossan (2020)
Investigation of the role of chromium reductase for Cr (VI) reduction by Pseudomonas species isolated from Cr (VI) contaminated effluent
P. A. Wani (2019)
Antioxidant and chromium reductase assisted chromium (VI) reduction and Cr (III) immobilization by the rhizospheric Bacillus helps in the remediation of Cr (VI) and growth promotion of soybean crop
P. A. Wani (2018)
Isolation and characterization of novel potent Cr(VI) reducing alkaliphilic Amphibacillus sp. KSUCr3 from hypersaline soda lakes
A. S. Ibrahim (2011)
Luteimonas aestuarii SA13A as a Novel Chromium Reducing Strain isolated from Tannery Effluent
Ankita Baljeet Singh Saharan (2017)
Experimental Investigation and Artificial Neural Network-Based Modeling of Batch Reduction of Hexavalent Chromium by Immobilized Cells of Newly Isolated Strain of Chromium-Resistant Bacteria
V. Shetty K. (2011)
Self‐Healing of Cracks in Concrete Using Bacillus Strains Encapsulated in Sodium Alginate Beads
Saman Shahid (2020)
Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1
Minyan He (2010)
Comparative Study of Cr(VI) Removal by Exiguobacterium sp. in Free and Immobilized Forms
R. Batool (2014)
Role of NADH-dependent chromium reductases, exopolysaccharides and antioxidants by Paenibacillus thiaminolyticus PS 5 against damage induced by reactive oxygen species
P. A. Wani (2020)
Comparative evaluations on bio-treatment of hexavalent chromate by resting cells of Pseudochrobactrum sp. and Proteus sp. in wastewater.
Shimei Ge (2013)
Biotransformation of Cr (VI) by Newly Invented Bacterial Consortium SN6
Suchi Dave (2018)
Draft Genome Sequence of Bacillus cereus TN10, a Chromium-Resistant and -Reducing Strain Isolated from Tannery Effluent
M. Hossain (2020)
Prospective of chromium (VI) reduction under in vitro and in vivo conditions and stimulation of antioxidant defense of cowpea under the exposure of Cr (VI)
P. A. Wani (2018)
Tailored zeolites for the removal of metal oxyanions: overcoming intrinsic limitations of zeolites.
H. Figueiredo (2014)
Biosynthesis of palladium nanoparticles using Shewanella loihica PV-4 for excellent catalytic reduction of chromium(VI)
W. Wang (2018)
Bioreduction of Cr (VI) by potent novel chromate resistant alkaliphilic Bacillus sp. strain KSUCr5 isolated from hypersaline Soda lakes
A. S. Ibrahim (2011)
Cr (VI) Reduction by Indigenous Bacillus Species PB5 Isolated from Contaminated Soil of Abeokuta Ogun State, Nigeria
P. A. Wani (2015)
Chromium-Resistant Bacteria and Their Environmental Condition for Hexavalent Chromium Removal: A Review
M. Narayani (2013)See more