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Characterization Of A Novel Glycine-rich Protein From The Cell Wall Of Maize Silk Tissues
T. Tao, T. Ouellet, K. Dadej, S. Miller, D. Johnson, J. Singh
Published 2006 · Medicine, Biology
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The isolation, characterization and regulation of expression of a maize silk-specific gene is described. zmgrp5 (Zea mays glycine-rich protein 5) encodes a 187 amino acid glycine-rich protein that displays developmentally regulated silk-specific expression. Northern, Western, in situ mRNA hybridization and transient gene expression analyses indicate that zmgrp5 is expressed in silk hair and in cells of the vascular bundle and pollen tube transmitting tissue elements. The protein is secreted into the extracellular matrix and is localized in the cell wall fraction mainly through interactions mediated by covalent disulphide bridges. Taken together, these results suggest that the protein may play a role in maintaining silk structure during development. This is the first documented isolation of a stigma-specific gene from maize, an important agronomic member of the Poaceae family.
This paper references
Molecular plant pathology : a practical approach
S. Gurr (1992)
Glycine‐rich cell wall proteins in bean: gene structure and association of the protein with the vascular system.
B. Keller (1988)
A new structural element containing glycine-rich proteins and rhamnogalacturonan I in the protoxylem of seed plants
U. Ryser (2004)
A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs.
I. Dasilva (2002)
THE POLLEN‐STIGMA INTERACTION IN THE GRASSES. 5. TISSUE ORGANISATION AND CYTOCHEMISTRY OF THE STIGMA (“SILK”) OF ZEA MAYS L.
Y. Heslop-Harrison (1984)
Redox state and peroxidase system in sunflower plants exposed to ozone.
Detection of gene expression patterns in various plant tissues using nonradioactive mRNA in situ hybridization
GG Shu (1999)
RNA binding characteristics of a 16 kDa glycine-rich protein from maize.
M. Ludevid (1992)
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
R. Jefferson (1987)
Development of the pistillate spikelet and fertilization in Zea mays L
EC Miller (1919)
A novel glycine-rich protein is associated with starch grain accumulation during anther development.
A. Mousavi (1999)
Direct ligand–receptor complex interaction controls Brassica self-incompatibility
S. Takayama (2001)
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
M. Fromm (1990)
Ultrastructural Localization of a Bean Glycine-Rich Protein in Unlignified Primary Walls of Protoxylem Cells.
U. Ryser (1992)
Identification, sequence analysis and expression studies of novel anther-specific genes of Arabidopsis thaliana
P. Rubinelli (2004)
Effect of silk age on resistance of maize to Fusarium graminearum
L. Reid (1992)
Surfaces and Secretions in the Pollen–Stigma Interaction: A Brief Review
J. Heslop-harrison (1985)
Impact of Western Corn Rootworm (Coleoptera: Chrysomelidae) on Sweet Corn and Evaluation of Insecticidal and Cultural Control Options
M. P. Hoffmann (2000)
Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation
D. Xu (2004)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
S. Clough (1998)
Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize.
E. Dennis (1984)
THE OXIDATIVE BURST IN PLANT DISEASE RESISTANCE.
C. Lamb (1997)
Hydrophobic interactions of the structural protein GRP1.8 in the cell wall of protoxylem elements.
C. Ringli (2001)
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
H. Nielsen (1997)
A simple and efficient method for isolating RNA from pine trees
S. Chang (2007)
A maize glycine-rich protein is synthesized in the lateral root cap and accumulates in the mucilage.
T. Matsuyama (1999)
Structure of cytochrome b5 and its topology in the microsomal membrane.
J. Ozols (1989)
Molecular cloning: a laboratory manual 3
J. Sambrook (2001)
Long range-inverse PCR (LR-IPCR): extending the useful range of inverse PCR.
B. Benkel (1996)
Isolation of an efficient actin promoter for use in rice transformation.
D. McElroy (1990)
Root-specific expression of a Zea mays gene encoding a novel glycine-rich protein, zmGRP3
M. Goddemeier (2004)
Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize.
E. Dennis (1985)
Protoxylem: the deposition of a network containing glycine-rich cell wall proteins starts in the cell corners in close association with the pectins of the middle lamella
U. Ryser (2002)
Evaluation of oils and microbial pathogens for control of lepidopteran pests of sweet corn in New England.
R. Hazzard (2003)
Two endogenous proteins that induce cell wall extension in plants.
S. McQueen-Mason (1992)
Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins.
J. V. Frangioni (1993)
THE POLLEN‐STIGMA INTERACTION IN THE GRASSES. 2. POLLEN‐TUBE PENETRATION AND THE STIGMA RESPONSE IN SECALE
J. Heslop-Harrison (1981)
ULTRASTRUCTURAL STUDIES ON STYLES AND POLLEN TUBES OF ZEA MAYS L. GENERAL SURVEY ON POLLEN TUBE GROWTH IN VIVO
M. Kroh (1979)
A. Mousavi (2005)
In-situ hybridisation in plants
David Jackson (1992)
Structural cell-wall proteins in protoxylem development: evidence for a repair process mediated by a glycine-rich protein.
U. Ryser (1997)
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E. T. Johnson (2007)
Comparative studies on the constituents, antioxidant and anticancer activities of extracts from different varieties of corn silk.
J. Tian (2013)
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
Wanying Li (2018)
Drought affects abortion of reproductive organs by exacerbating developmentally driven processes via expansive growth and hydraulics
O. Turc (2018)
Plant extracellular matrix metalloproteinases.
B. Flinn (2008)
Characterization and Expression Analysis of Four Glycine-Rich RNA-Binding Proteins Involved in Osmotic Response in Tobacco (Nicotiana tabacum cv. Xanthi)
X. Chen (2010)
Cell wall Glycine-rich Protein2 is involved in tapetal differentiation and pollen maturation.
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Bing Wang (2017)
The growth of vegetative and reproductive structures (leaves and silks) respond similarly to hydraulic cues in maize.
O. Turc (2016)