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Agrobacterium-mediated Transformation Of Citrange: Factors Affecting Transformation And Regeneration

M. Cervera, J. Pina, J. Juárez, L. Navarro, L. Peña
Published 1998 · Biology, Medicine

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Abstract The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. Osbeck×Poncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during cocultivation, cocultivation on a medium rich in auxins, postcultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day cocultivation on a medium rich in auxins improved transformation frequencies, since it increased the number of dividing cells competent for transformation, at the cut ends of the explants. Exposure of explants to darkness for 4 weeks on selection medium resulted in further callus development and increased the regeneration frequency of transgenic shoots. Furthermore, this treatment drastically reduced the number of regenerated escape shoots. A transformation efficiency of 41.3% was achieved using the optimized transformation procedure.
This paper references
A revised medium for the growth and bioassay with tobacco tissue culture
T. Murashige (1962)
Improvement of shoot-tip grafting in vitro for virus-free citrus.
L. Navarro (1975)
A plant DNA miniprepar - ation : version II
SL Dellaporta (1983)
Transferring genes into plants
RB Horsch (1985)
10.1038/NBT0787-726
Efficient Transfer of a Glyphosate Tolerance Gene into Tomato Using a Binary Agrobacterium Tumefaciens Vector
J. Fillatti (1987)
10.1038/NBT1190-1041
Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L.
M. G. Mullins (1990)
10.1038/NBT0991-858
Transformation of Melon (Cucumis melo L.) and Expression from the Cauliflower Mosaic Virus 35S Promoter in Transgenic Melon Plants
J. Dong (1991)
10.1038/NBT0991-853
Agrobacterium-Mediated Transformation of Plum (Prunus domestica L.) Hypocotyl Slices and Regeneration of Transgenic Plants
S. Mante (1991)
10.1038/NBT0991-864
Agrobacterium-Mediated Transformation of Carnation (Dianthus caryophyllus L.)
Chin-Yi Lu (1991)
10.1016/S0176-1617(11)80370-6
Transformation of Apple Rootstock M26 with Agrobacterium tumefaciens
G. Maheswaran (1992)
10.1016/0168-9452(94)04044-H
Growth of healthy and viroid-infected tomato cells in vitro
N. Durán-Vila (1995)
10.1016/0168-9452(94)04021-8
High efficiency Agrobacterium-mediated transformation and regeneration of citrus
L. Peña (1995)
10.1007/s002990050216
Efficient Agrobacterium-mediated transformation and recovery of transgenic plants from pear (Pyrus communis L.)
F. Mourgues (1996)
10.1007/s002990050311
Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration
L. Peña (1997)
10.1007/s002990050313
Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus
M. A. Gutíerrez-E. (1997)
10.1006/PMPP.1997.0135
Virulence and supervirulence ofAgrobacterium tumefaciensin woody fruit plants
M. Cervera (1998)
Ag - robacterium - mediated transformation and regeneration of Popu
JJ Fillatti



This paper is referenced by
10.1007/978-981-15-5932-7
Genetically Modified Crops: Current Status, Prospects and Challenges Volume 2
P. B. K. Kishor (2021)
10.3390/plants9080938
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus
L. Poles (2020)
Agrobacterium-mediated transformation of sour orange (Citrus aurantium L.) using gene encoding Citrus Tristeza Virus coat protein
M. Sohani (2020)
10.1080/14620316.2020.1814876
Effect of antibiotics on Agrobacterium-mediated transformation from anther derived embryos of Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’
Lihua Lin (2020)
10.1080/10826068.2020.1805757
Establishment and elicitation of transgenic root culture of Plantago lanceolata and evaluation of its anti-bacterial and cytotoxicity activity
Samaneh Rahamooz-Haghighi (2020)
10.3389/fpls.2020.01234
New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review
Fabrizio Salonia (2020)
10.1016/j.jbiotec.2020.09.010
Transgenic Citrange troyer rootstocks overexpressing antimicrobial potato Snakin-1 show reduced citrus canker disease symptoms.
G. Conti (2020)
10.3389/fpls.2020.523748
DNA Methylation Silences Exogenous Gene Expression in Transgenic Birch Progeny
Minghao Ma (2020)
10.1007/978-981-15-5932-7_7
Genetically Modified Citrus: Current Status, Prospects, and Future Challenges
Sameena E. Tanwir (2020)
10.1007/s11627-019-10011-9
Improved protocol for the transformation of adult Citrus sinensis Osbeck ‘Tarocco’ blood orange tissues
Aihong Peng (2019)
10.3389/fpls.2019.00312
The Rapid Methylation of T-DNAs Upon Agrobacterium Inoculation in Plant Leaves
Joshua G. Philips (2019)
10.1007/s42729-019-00130-y
Increased Drought and Salinity Tolerance in Citrus aurantifolia (Mexican Lemon) Plants Overexpressing Arabidopsis CBF3 Gene
J. L. Romero-Romero (2019)
10.1007/s002990100382
Transgenic tea [Camellia sinensis (L.) O. Kuntze cv. Kangra Jat] plants obtained by Agrobacterium-mediated transformation of somatic embryos
T. Mondal (2019)
10.3389/fpls.2019.00226
Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges
Guo-Qing Song (2019)
10.1007/s13205-018-1231-1
Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)]
S. Karthik (2018)
Determination of minimal inhibitory concentration of kanamycin as selective agents and marker genes for use in Agrobacterium mediated transformation in sugarcane
Jyoti Rastogi (2018)
Obtención de plantas transgénicas de Digitalis purpurea L. (Plantaginaceae) que contienen el gen vep1 de Arabidopsis thaliana L.
E. Antón (2018)
10.1007/s11240-017-1271-y
Construction of high efficiency regeneration and transformation systems of Pyrus ussuriensis Maxim
Y. Yang (2017)
5 Citrus Transformation : Challenges and Prospects
V. Febres (2017)
10.1016/j.pestbp.2016.07.006
Expression of Bacillus thuringiensis cytolytic toxin (Cyt2Ca1) in citrus roots to control Diaprepes abbreviatus larvae.
S. Mahmoud (2017)
10.25125/AGRICULTURE-JOURNAL-IJOEAR-SEP-2017-10
The utilities of Citrus tissue culture
F. Chamandoosti (2017)
10.1007/s11627-016-9774-5
Low Agrobacterium tumefaciens inoculum levels and a long co-culture period lead to reduced plant defense responses and increase transgenic shoot production of sunflower (Helianthus annuus L.)
Zhifen Zhang (2016)
Use of genetic transformation technology in oil crops: soybean and sunflower
Zhifen Zhang (2016)
10.1007/978-1-4939-3061-6_13
Somatic Embryogenesis: Still a Relevant Technique in Citrus Improvement.
A. A. Omar (2016)
Development of an efficient method for regeneration and Agrobacterium -mediated transformation of cotton ( Gossypium hirsutum L.) cv. HS6
G. Wamiq (2016)
Genetic enhancement of banana: A Biotechnological approach to Attain a Higher Yield
Sonia S Anand (2016)
10.5772/66465
New Approaches to Agrobacterium tumefaciens-Mediated Gene Transfer to Plants
M. Yıldız (2016)
10.37855/JAH.2015.V17I02.24
Effect of various factors on shoot regeneration from citrus epicotyl explants
R. Niedz (2015)
Agrobacterium-mediated genetic transformation of date palm ( Phoenix dactylifera L.) cultivar "Khalasah" via somatic
J. Aslam (2015)
10.4236/ABB.2015.611069
Agrobacterium -Mediated Transformation of Mexican Lime ( Citrus aurantifolia Swingle) Using Optimized Systems for Epicotyls and Cotyledons
M. L. P. Oliveira (2015)
10.1016/j.plaphy.2015.04.005
Molecular and physiological changes in response to salt stress in Citrus macrophylla W plants overexpressing Arabidopsis CBF3/DREB1A.
Ximena Alvarez-Gerding (2015)
STUDIES ON Agrobacterium- MEDIATED INSECT RESISTANCE GENE [cry1A(a)] TRANSFER IN CAULIFLOWER (Brassica oleracea L. var.botrytis)
Ayesh Gaur (2015)
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