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Developmental Regulation Of Pectic Epitopes During Potato Tuberisation

M. S. Bush, M. Marry, Max I. Huxham, M. Jarvis, M. McCann
Published 2001 · Biology, Medicine

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Abstract. We show, by immunogold labelling, that potato (Solanum tuberosum L. cv Karnico) pectic epitopes are developmentally regulated within regions of the stolon, in addition to showing tissue-specific differences in abundance and localisation. The (1→4)-β-D-galactan and (1→5)-α-arabinan epitopes demarcate two distinct zones within stolons; galactans are enriched in primary walls of elongating cells proximal to the stolon hook, whilst arabinans predominate in younger cells distal to the hook. Low-methoxyl homogalacturonan epitopes are concentrated in the middle lamella and show a proximo-distal gradient in stolons similar to that of galactans, whilst high-methoxyl homogalacturonan is uniformly abundant. Calcium pectate is restricted to the middle lamella at cell corners and pit fields. Calcium-binding sites are uniformly present in stolon cell walls, but their total density is reduced and they become localised to a few cell corners in mature tubers, as determined by image-electron energy loss spectroscopy. During the transition from elongation growth to isodiametric expansion during tuberisation of the stolon hook, there were no detectable changes in pectic epitope abundance or localisation. As tubers matured, all epitopes increased in abundance in parenchymal cell walls, except for calcium pectate. We conclude that potentially significant changes in pectic composition occur as young cells distal to the stolon hook move into the zone of cell elongation proximal to the hook.
This paper references
An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum)
D. Vreugdenhil (1989)
Immunolocalization of b-(1®4) and b-(1®6)-D-galactan epitopes in the cell wall and golgi stacks of developing¯ax root tissues
M Vicreâ (1998)
Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1[->]4)-[beta]-D-Galactan
L. Jones (1997)
Cross-Linking of Matrix Polymers in the Growing Cell Walls of Angiosperms
S. Fry (1986)
Early Tuber Development from Explanted Stolon Nodes of Solanum tuberosum var. Kennebec
R. Peterson (1979)
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth.
N. Carpita (1993)
Temporal and spatial regulation of pectic (1-->4)-beta-D-galactan in cell walls of developing pea cotyledons: implications for mechanical properties.
L. McCartney (2000)
Reduction in Pectin Methylesterase Activity Modifies Tissue Integrity and Cation Levels in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits
D. Tieman (1994)
Immunolocalization of pectin methylesterases in the hypocotyl tissues of flax
M. Quentin (1997)
Cell division and cell enlargement during potato tuber formation
X. Xu (1998)
Growth and cell wall changes in azuki bean epicotyls I. Changes in wall polysaccharides during intact growth
K. Nishitani (1979)
Generation of a monoclonal antibody specific to (1→5)-α-l-arabinan
W. Willats (1998)
Immunogold localisation of pectin methylesterases in the cortical tissues of¯ax hypocotyl
O Morvan (1998)
The Cytoskeletal basis of plant growth and form
C. Lloyd (1991)
Remodelling pectin structure in potato
J. Vincken (2000)
Estimation of Polymer Rigidity in Cell Walls of Growing and Nongrowing Celery Collenchyma by Solid-State Nuclear Magnetic Resonance in Vivo
K. M. Fenwick (1997)
The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro
Xu (1998)
Changes in cell wall architecture during cell elongation
M. McCann (1994)
Pectic epitopes are dierentially expressed in the cell walls of potato (Solanum tuberosum) tubers
O Baron-Epel (1988)
Occurrence of pectic hairy regions in various plant cell wall materials and their degradability by rhamnogalacturonase
H. Schols (1994)
Quantitative trait loci for polyamine content in an RFLP-mapped potato population and their relationship to tuberization
P. J. Davies (1999)
Monoclonal Antibodies against Pectin: Recognition of a Conformation Induced by Calcium.
F. Liners (1989)
In vitro assembly of cellulose/xyloglucan networks: ultrastructural and molecular aspects
S. Whitney (1995)
Gibberellin regulation of root growth with change in galactose content of cell walls in Pisum sativum
E. Tanimoto (1988)
Assembly and enlargement of the primary cell wall in plants.
D. Cosgrove (1997)
Composition, Properties and Localisation of Pectins in Young and Mature Cells of the Mung Bean Hypocotyl :
R. Goldberg (1986)
Expression of a Petunia in¯ata pectin methyl esterase in Solanum tuberosum L. enhances stem elongation and modi®es cation distribution
J Pilling (2000)
Methyl-esterification, de-esterification and gelation of pectins in the primary cell wall
R. Goldberg (1996)
Development of arabinans and galactans during the maturation of hypocotyl cells of mung bean (Vigna radiata Wilczek)
C. Penhoat (1987)
Mobilityresolved 13 C-NMR spectroscopy of primary plant cell walls
T J Foster (1996)
Orientation of macromolecules in the walls of elongating carrot cells.
M. McCann (1993)
Intercellular separation forces generated by intracellular pressure
M. Jarvis (1998)
Monoclonal Antibodies against Pectin
J. Letesson (1989)
Phase Separation of Plant Cell Wall Polysaccharides and Its Implications for Cell Wall Assembly
A. MacDougall (1997)
Functional compartmentation of the Golgi apparatus of plant cells : immunocytochemical analysis of high-pressure frozen- and freeze-substituted sycamore maple suspension culture cells.
G. F. Zhang (1992)
Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation.
W. Willats (2000)
The use of Antibodies to Study the Architecture and Developmental Regulation of Plant Cell Walls
Knox Jp (1997)
Side chains of pectic polysaccharides are regulated in relation to cell proliferation and cell differentiation
Willats (1999)
The Pore Size of Non-Graminaceous Plant Cell Walls Is Rapidly Decreased by Borate Ester Cross-Linking of the Pectic Polysaccharide Rhamnogalacturonan II.
Fleischer (1999)
Common components of the infection thread matrix and the intercellular space identified by immunocytochemical analysis of pea nodules and uninfected roots
K. VandenBosch (1989)
Characterization of pectin gelation under conditions of low water activity, by circular dichroism, competitive inhibition and mechanical properties
E. Morris (1980)
Changes in pectin structure and localization during the growth of unadapted and NaCl-adapted tobacco cells
M. McCann (1994)
Changes in Pectin Structure during Epidermal Cell Elongation in Pea (Pisum sativum) and Its Implications for Cell Wall Architecture
T. Fujino (1998)
Immunolocalisation of pectin methylesterases in the hypocotyl tissues of¯ax
M Quentin (1997)
Reduction in pectin methylesterase activity modi®es tissue integrity and cation levels in ripening tomato
D Tieman (1994)
Quantitative trait loci for polyamine content in an RFLP-mapped potato population and their relationship to tuberisation
P J Davies (1999)
Electron-energy-loss spectroscopic imaging of calcium and nitrogen in the cell walls of apple fruits
I. M. Huxham (1999)

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Remodeling of pectin and hemicelluloses in tomato pericarp during fruit growth.
F. Guillon (2017)
Die Neigung zu Schwarzfleckigkeit bei Kartoffeln (Solanum tuberosum) unter besonderer Berücksichtigung der Knollenphysiologie
Antje Wulkow (2009)
Plant cell walls: Supramolecular assemblies
M. Jarvis (2011)
Rhamnogalacturonan lyase: a pectin modification enzyme of higher plants
Verónica-Alhelí Ochoa-Jiménez (2018)
Microarray analysis of developing flax hypocotyls identifies novel transcripts correlated with specific stages of phloem fibre differentiation.
Melissa J. Roach (2008)
Understanding mechanisms and identifying markers for the onset of senescent sweetening of potato (Solanum tuberosum)
C. Carvalho (2017)
Use of energy filtering transmission electron microscopy for image generation and element analysis in plant organisms.
U. Luetz-Meindl (2007)
Roles of pectin in biomass yield and processing for biofuels
Chaowen Xiao (2013)
Pectin biosynthesis - identification of glycosyltransferases
Jesper Harholt (2015)
Characterization of the putative catalytic domain of two xyloglucan xylosyltransferases (XXTs) involved in the biosynthesis of xyloglucan in Arabidopsis thaliana
R. Morris (2013)
Pectin — the Hairy Thing
J. Vincken (2003)
Conformation and mobility of the arabinan and galactan side-chains of pectin.
Marie-Ann Ha (2005)
Hormones, Signals and Target Cells in Plant Development: Population Diversity of Cell Types and Target Identification in Higher Plants
D. Osborne (2005)
Flexibility of Cell Types and the Target Cell Status
D. Osborne (2005)
Mechanical Properties of Plant Cell Walls Probed by Relaxation Spectra1[W][OA]
Steen Laugesen Hansen (2010)
An Improved Method for the Fixation, Embedding and Immunofluorescence Labeling of Resin-Embedded Plant Tissue
W. Chen (2007)
Pectin Metabolism and Assembly in the Cell Wall of the Charophyte Green Alga Penium margaritaceum1[W][OPEN]
D. Domozych (2014)
Direct Interference with Rhamnogalacturonan I Biosynthesis in Golgi Vesicles1
Michael Skjøt (2002)
Intercellular adhesion and cell separation in plants
M. Jarvis (2003)
Hormones, Signals and Target Cells in Plant Development
D. Osborne (2005)
Microarray analysis of flax (Linum usitatissimum L.) stems identifies transcripts enriched in fibre-bearing phloem tissues
M. J. Roach (2007)
Approaches to understanding the functional architecture of the plant cell wall.
M. McCann (2001)
Hormones, Signals and Target Cells in Plant Development: Hormones and Signals: Identification and Description of Signalling Molecules
D. Osborne (2005)
Structural characterization of native pectins
G. J. Coenen (2007)
Spatial regulation of cell-wall structure in response to heavy metal stress: cadmium-induced alteration of the methyl-esterification pattern of homogalacturonans.
O. Douchiche (2010)
Potato tuber pectin structure is influenced by pectin methyl esterase activity and impacts on cooked potato texture
H. A. Ross (2011)
The structure, function, and biosynthesis of plant cell wall pectic polysaccharides.
K. Caffall (2009)
Histological analysis of the maturation of native and wound periderm in potato (Solanum tuberosum L.) Tuber.
Robert P. Sabba (2002)
Hormones, Signals and Target Cells in Plant Development: The Mechanisms of Target Cell Perception and Response to Specific Signals
D. Osborne (2005)
How to let go: pectin and plant cell adhesion
Firas Bou Daher (2015)
Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood
H. Yang (2019)
In muro fragmentation of the rhamnogalacturonan I backbone in potato (Solanum tuberosum L.) results in a reduction and altered location of the galactan and arabinan side-chains and abnormal periderm development.
R. Oomen (2002)
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