Online citations, reference lists, and bibliographies.
← Back to Search

A Pqr2 Mutant Encodes A Defective Polyamine Transporter And Is Negatively Affected By ABA For Paraquat Resistance In Arabidopsis Thaliana

Shuchao Dong, Huizhen Hu, Youmei Wang, Zhengdan Xu, Yi Zha, X. Cai, L. Peng, S. Feng
Published 2016 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Despite the paraquat-resistant mutants that have been reported in plants, this study identified a novel A. thaliana mutant (pqr2) from an XVE inducible activation library based on its resistance to 2 μM paraquat. The pqr2 mutant exhibited a termination mutation in the exon of AT1G31830/PAR1/PQR2, encoded a polyamine uptake transporter AtPUT2/PAR1/PQR2. The PQR2 mutation could largely reduce superoxide accumulation and cell death in the pqr2 plants under paraquat treatment. Moreover, compared with wild type, the pqr2 mutant exhibited much reduced tolerance to putrescine, a classic polyamine compound, which confirmed that PQR2 encoded a defective polyamine transporter. Notably, co-treated with ABA and paraquat, both pqr2 mutant and wild type exhibited a lethal phenotype from seed germination, but the wild type like pqr2 mutant, could remain paraquat-resistance while co-treated with high dosage of Na2WO4, an ABA synthesis inhibitor. Gene expression analysis suggested that ABA signaling should widely regulate paraquat-responsive genes distinctively in wild type and pqr2 mutant. Hence, this study has for the first time reported about ABA negative effect on paraquat-resistance in A. thaliana, providing insight into the ABA signaling involved in the oxidative stress responses induced by paraquat in plants.
This paper references
10.1007/s00425-012-1668-0
Kinetic and phylogenetic analysis of plant polyamine uptake transporters
Vaishali Mulangi (2012)
10.1111/WRE.12012
Resistance to paraquat in Mazus pumilus
K. Tsuji (2013)
10.1111/J.1439-037X.2004.00106.X
Protective Effects of Polyamines against Oxidative Stress Induced by Water and Cold Stress in Chickpea
H. Nayyar (2004)
10.1080/10408440701669959
Paraquat Poisonings: Mechanisms of Lung Toxicity, Clinical Features, and Treatment
R. Dinis-Oliveira (2008)
10.1126/SCIENCE.1355616
Protein oxidation and aging.
E. Stadtman (2006)
10.1093/OXFORDJOURNALS.PCP.A029463
Polyamines and paraquat toxicity in Arabidopsis thaliana.
J. Kurepa (1998)
10.1113/expphysiol.1997.sp004024
Oxidative stress: oxidants and antioxidants
H. Sies (1997)
10.1038/cr.2009.117
The Arabidopsis PARAQUAT RESISTANT2 gene encodes an S-nitrosoglutathione reductase that is a key regulator of cell death
Ruiqiang Chen (2009)
10.1007/s12038-012-9218-2
DNA damage by reactive species: Mechanisms, mutation and repair
N. Jena (2012)
10.1016/J.PESTBP.2004.07.002
Polyamines can inhibit paraquat toxicity and translocation in the broadleaf weed Arctotheca calendula
C. Soar (2004)
10.1046/J.1365-313X.2000.00723.X
Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O2 is the likely intermediary signaling molecule for the response.
L. Guan (2000)
10.1038/cr.2008.22
Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in Arabidopsis
L. Shi (2008)
10.1073/pnas.1121406109
Natural variation in a polyamine transporter determines paraquat tolerance in Arabidopsis
M. Fujita (2012)
10.1042/BSS0610001
Oxidative stress: the paradox of aerobic life.
K. Davies (1995)
10.1371/journal.pone.0122690
Transcriptomic Profiling of Arabidopsis thaliana Mutant pad2.1 in Response to Combined Cold and Osmotic Stress
D. Kumar (2015)
10.1093/jxb/eru217
H2O2 mediates the crosstalk of brassinosteroid and abscisic acid in tomato responses to heat and oxidative stresses
J. Zhou (2014)
10.1104/PP.90.4.1267
Lethal hydroxyl radical production in paraquat-treated plants.
C. Babbs (1989)
Polyamines: molecules with
P Carrasco (2010)
10.4161/psb.5.1.10291
Polyamines and abiotic stress tolerance in plants
S. Gill (2010)
10.1111/pce.12355
A cell type-specific view on the translation of mRNAs from ROS-responsive genes upon paraquat treatment of Arabidopsis thaliana leaves.
Maria Benina (2015)
10.1111/pce.12204
Polyamines reprogram oxidative and nitrosative status and the proteome of citrus plants exposed to salinity stress.
G. Tanou (2014)
10.1023/A:1006316926002
Relationship between polyamines and paraquat toxicity in sunflower leaf discs
M. Benavides (2004)
10.1007/s00425-010-1130-0
Polyamines: molecules with regulatory functions in plant abiotic stress tolerance
R. Alcázar (2010)
10.1105/tpc.114.133090
The Roles of ROS and ABA in Systemic Acquired Acclimation[OPEN]
R. Mittler (2015)
10.1111/j.1744-7909.2009.00868.x
Induction of protection against paraquat-induced oxidative damage by abscisic acid in maize leaves is mediated through mitogen-activated protein kinase.
Haidong Ding (2009)
10.1089/omi.2011.0084
Interactions between polyamines and abiotic stress pathway responses unraveled by transcriptome analysis of polyamine overproducers.
Francisco Marco (2011)
10.1111/j.1365-313X.2011.04830.x
Loss of AtPDR11, a plasma membrane-localized ABC transporter, confers paraquat tolerance in Arabidopsis thaliana.
Jing Xi (2012)
10.1007/s00018-003-2332-4
Polyamine-dependent gene expression
A. Childs (2003)
10.1093/emboj/cdg277
NADPH oxidase AtrbohD and AtrbohF genes function in ROS‐dependent ABA signaling in Arabidopsis
J. Kwak (2003)
Mechanism of paraquat resistance - from the antioxidant enzymes to the transporters
Z. Szigeti (2005)
10.1023/B:MCBI.0000038227.91813.79
Hydroxyl radical scavenging and singlet oxygen quenching properties of polyamines
K. Das (2004)
PARAQUAT RESISTANT 1 , a Golgi-Localized Putative Transporter Protein , Is Involved in Intracellular Transport of Paraquat 1 [ C ] [ W ]
J. Li (2013)
10.1104/PP.117.1.217
Two structurally similar maize cytosolic superoxide dismutase genes, Sod4 and Sod4A, respond differentially to abscisic acid and high osmoticum.
L. Guan (1998)
10.1016/J.PLANTSCI.2003.09.019
The important roles of reactive oxygen species in the relationship between ethylene and polyamines in leaves of spring wheat seedlings under root osmotic stress
Chao-zhou Li (2004)
Cross-talk between reactive oxygen
I Pottosin (2014)
10.1007/s00425-014-2055-9
The roles of polyamines during the lifespan of plants: from development to stress
A. Tiburcio (2014)
10.1016/S0031-9422(00)85482-5
Radical scavenging properties of polyamines
G. Drolet (1986)
10.1080/10715769900301541
Differential gene expressions of rice superoxide dismutase isoforms to oxidative and environmental stresses.
H. Kaminaka (1999)
10.1104/pp.106.1.173
Differential Accumulation of Manganese-Superoxide Dismutase Transcripts in Maize in Response to Abscisic Acid and High Osmoticum
D. Zhu (1994)
10.1104/pp.115.4.1341
Association of Caffeoyl Coenzyme A 3-O-Methyltransferase Expression with Lignifying Tissues in Several Dicot Plants
Z. Ye (1997)
10.1016/j.biocel.2009.07.009
Modulation of cellular function by polyamines.
K. Igarashi (2010)
10.1093/pcp/pcu032
Identification of polyamine transporters in plants: paraquat transport provides crucial clues.
M. Fujita (2014)
10.1046/J.1365-313X.1998.00343.X
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
S. Clough (1998)
10.1016/S0031-9422(00)97805-1
Polyamines as radical scavengers and protectants against ozone damage
W. Bors (1989)
10.1016/J.JPLPH.2007.02.005
Exogenous spermidine differentially alters activities of some scavenging system enzymes, H(2)O(2) and superoxide radical levels in water-stressed cucumber leaves.
J. Kubiš (2008)
10.1007/s11033-014-3396-y
Gene expression profiles of arabidopsis under the stress of methyl viologen: a microarray analysis
Hong-juan Han (2014)
10.1007/s10725-005-6395-0
Polyamines reduce salt-induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in Virginia pine
W. Tang (2005)
10.1016/0005-2728(90)90213-N
The sites of electron donation of Photosystem I to methyl viologen
T. Fujii (1990)
A cell type-specific view on the translation of mRNAs from ROS-responsive genes upon paraquat treatment of Arabidopsis thaliana
M Benina (2014)
10.1104/pp.113.213892
PARAQUAT RESISTANT1, a Golgi-Localized Putative Transporter Protein, Is Involved in Intracellular Transport of Paraquat1[C][W]
J. Li (2013)
10.1046/J.1365-313X.2000.00868.X
Technical advance: An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants.
J. Zuo (2000)
10.1023/A:1026532223173
Programmed cell death of tracheary elements as a paradigm in plants
H. Fukuda (2004)
10.1093/jxb/ert423
Cross-talk between reactive oxygen species and polyamines in regulation of ion transport across the plasma membrane: implications for plant adaptive responses.
I. Pottosin (2014)
10.1038/nature01485
Reactive oxygen species produced by NADPH oxidase regulate plant cell growth
J. Foreman (2003)
Paraquat : model for oxidantinitiated toxic
JS Bus (1984)
10.1104/PP.99.4.1400
Transport Interactions between Paraquat and Polyamines in Roots of Intact Maize Seedlings.
J. Hart (1992)
10.1104/pp.102.2.467
Compartmentation Analysis of Paraquat Fluxes in Maize Roots as a Means of Estimating the Rate of Vacuolar Accumulation and Translocation to Shoots
J. M. Ditomaso (1993)
10.1289/EHP.845537
Paraquat: model for oxidant-initiated toxicity.
J. Bus (1984)
10.1016/J.BIOCEL.2006.07.001
Free radicals and antioxidants in normal physiological functions and human disease.
M. Valko (2007)
10.1111/j.1582-4934.2005.tb00493.x
Polyamines and apoptosis
N. Seiler (2005)
10.1104/pp.115.4.1443
Constitutively Elevated Levels of Putrescine and Putrescine-Generating Enzymes Correlated with Oxidant Stress Resistance in Conyza bonariensis and Wheat
B. Ye (1997)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar