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Development Of An Efficient Protocol For Plant Regeneration From Nodal Explants Of Recalcitrant Bamboo (Bambusa Arundinacea Retz. Willd) And Assessment Of Genetic Fidelity By DNA Markers

K. Kalaiarasi, P. Sangeetha, S. Subramaniam, P. Venkatachalam
Published 2014 · Biology

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AbstractThe present study describes an efficient method for in vitro plant regeneration in B. arundinacea through axillary shoot bud proliferation. Nodal explants were excised, cultured on MS medium containing different concentrations of 6-benzylaminopurine (BAP), kinetin (KIN) (0.5–5.0 mg l−1) alone and/or in combinations with KIN/BAP (0.5 mg l−1). The highest frequency (91.5 %) of multiple shoot bud induction with maximum number of shoots (85 shoots/explant) was noticed on MS medium + 3.0 mg l−1 BAP + 0.5 mg l−1 KIN. The regenerated multiple shoots were elongated on MS medium + 4.0 mg l−1 KIN + 2.0 mg l−1 gibberellic acid (GA3) with maximum shoot length (4.9 cm). The elongated shoots were transferred to MS medium containing indole-3 butyric acid (IBA; 0.5–5.0 mg l−1) alone and/or in combination with 0.5 mg l−1 KIN and BAP. Highest frequency of rooting (75 %) was obtained on half-strength MS medium + 2.0 mg l−1 IBA + 0.5 mg l−1 KIN. After hardening, the plantlets were shifted to the green house and subsequently established in the field conditions with 90 % survival rate. random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of the regenerants. RAPD profiles generated from the regenerated plants were found to be monomorphic, similar to the control. Results confirmed that the regenerated plants were true-to-type in nature and the developed micropropagation protocol could be used for large scale plant production of B. arundinacea.
This paper references
A revised medium for the growth and bioassay with tobacco tissue culture
T. Murashige (1962)
Rapid multiplication of bamboo by tissue culture
A. L. Nadgir (1984)
Inventory and resources of bamboos
YML Sharma (1987)
10.1016/0378-8741(89)90019-6
Antifertility effect of Bambusa arundinacea shoot extracts in male rats.
G. Vanithakumari (1989)
Isolation of plant DNA from fresh tissue
J. Doyle (1990)
Propagation of bamboo through tissue culture
KB Kumar (1994)
Country report. India.
K. A. Pisharoti (1995)
10.1177/102538239500200222
Country report. India.
Pisharoti Ka (1995)
10.1016/S0168-9452(97)00185-4
Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms
S. Ramanayake (1997)
10.1007/s002990050453
Micropropagation of Bambusa edulis through nodal explants of field-grown culms and flowering of regenerated plantlets
C. Lin (1998)
Augmenting in vitro shoot multiplication by vipul (triacontanol) and adventitious rhizogenesis by rice bran extract in Dendrocalamus strictus.
Y. Mishra (2001)
10.17660/ACTAHORTIC.2001.552.22
TISSUE CULTURE STRATEGIES FOR GENETIC IMPROVEMENT OF BAMBOO
J. Gielis (2001)
10.1300/J091v14n02_06
Rapid Micropropagation of Edible Bamboo Dendrocalamus asper
I. D. Arya (2001)
Somatic embryogenesis and its conver- sion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et Arn. Ex Munro
S. Godbole (2002)
Rapid mass multiplication of edible bamboo Dendrocalamus asper
S Arya (2002)
Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et
S Godbole (2002)
10.1016/S0378-8741(03)00183-1
Antiinflammatory and antiulcer activities of Bambusa arundinacea.
M. Muniappan (2003)
10.1007/BF00232266
In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation
Sanjay Saxena (2004)
10.1023/A:1016582732531
In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn. Ex Munro*
A. Sood (2004)
10.1007/s11240-004-3017-x
In vitro regeneration of Bambusa balcooa Roxb.: Factors affecting changes of morphogenetic competence in the axillary buds
M. Das (2004)
10.1016/J.SCIENTA.2006.06.016
In vitro shoot proliferation and enhancement of rooting for the large-scale propagation of yellow bamboo (Bambusa vulgaris ‘Striata’)
S. Ramanayake (2006)
10.1007/s11240-006-9120-4
In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation
V. M. Jimenez (2006)
Tissue Culture Technology for Rapid Multiplication of Dendrocalamus giganteus Munro
S. Arya (2006)
10.1079/IVP2004625
Micropropagation of Pseudoxytenanthera stocksii Munro
Sanjaya (2007)
10.1007/s11816-007-0020-9
In vitro plantlet regeneration from nodal explants of field-grown culms in Bambusa glaucescens Willd.
F. Shirin (2007)
Micropropagation of Two Economically Important Bamboos : Drepanostachyum falcatum (NEES) Keng and Bambusa balcooa Roxb.
S. Arya (2008)
10.1007/s11738-009-0311-6
Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation
R. Agnihotri (2009)
Callus induction and plantlet regeneration of Bambusa multiplex.
Yuan Jin-ling (2009)
10.5897/AJB10.1012
Biochemical changes in low-salt solid-state fermented soy sauce
Zhang Yan-fang (2010)
10.1016/j.jep.2010.01.045
Protective effects of the pyrolyzates derived from bamboo against neuronal damage and hematoaggregation.
E. Hong (2010)
Effect of Cytokinin and Auxins on Meristem Culture of Bambusa Arundinacea
Satish Nayak (2010)
10.4314/AJB.V9I45
Antibacterial activity of water-phase extracts from bamboo shavings against food spoilage microorganisms
J. Zhang (2010)
10.1007/s11240-010-9783-8
Somatic embryogenesis and organogenesis in Dendrocalamus hamiltonii
N. Zhang (2010)
10.1007/s10342-010-0462-4
Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers
Rupali Mehta (2010)
Direct shoot organogenesis and plant regeneration in rough lemon (Citrus jambhiri Lush.).
H. K. Saini (2010)
10.3724/SP.J.1259.2011.00170
A Preliminary Study of Somatic Embryogenesis of Phyllostachys violascens In Vitro : A Preliminary Study of Somatic Embryogenesis of Phyllostachys violascens In Vitro
Pei Hai-yan (2011)
Evaluation of clonal fidelity of in vitro raised plants of Guadua angustifolia Kunth using DNA-based markers
Harleen Kaur Nadha (2011)
10.1007/s11627-011-9403-2
Micropropagation of Bambusa balcooa Roxb. through axillary shoot proliferation
D. Negi (2011)
10.1111/J.1541-4337.2011.00147.X
Nutritional Properties of Bamboo Shoots: Potential and Prospects for Utilization as a Health Food
Nirmala Chongtham (2011)
Anti - diabetic activity of Bambusa arundinacea seed extracts on alloxan induced diabetic rats
SP Macharla (2011)
Effect of benzyl amino purine and gibberellic acid on in vitro shoot multiplication and elongation of Dalbergia latifolia Roxb.: An important multipurpose tree
Anitha Boga (2012)
10.1007/s11738-012-1084-x
Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. & Schult. F.) Backer ex K. Heyne using DNA-based markers
S. Singh (2012)
10.22270/JDDT.V2I3.150
AMELIORATIVE EFFECT OF BAMBUSA ARUNDINACEA AGAINST ADJUVANT ARTHRITIS-WITH SPECIAL REFERENCE TO BONE EROSION AND TROPICAL SPLENOMEGALY
J. D. Rathod (2012)
10.4081/PB.2012.E6
In vitro cloning of Bambusa pallida Munro through axillary shoot proliferation and evaluation of genetic fidelity by random amplified polymorphic DNA markers
D. B. Beena (2012)
10.1155/2014/943054
Influence of Cytokinins in Combination with GA3 on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis (L.) O. Kuntze)
R. Gonbad (2014)
Influence of cytokinins in combination with GA 3 on shoot multiplication and elongation of tea clone Iran
Ra Gonbad (2014)
Agroforest Syst
(2014)
( eds ) Proceedings of the twentieth international eucarpia symposium
V Debergh



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