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Arabidopsis MEKK1 Can Take A Short Cut: It Can Directly Interact With Senescence-related WRKY53 Transcription Factor On The Protein Level And Can Bind To Its Promoter

Ying Miao, Thomas M. Laun, A. Smykowski, U. Zentgraf
Published 2007 · Biology, Medicine

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Despite the importance of the senescence processes in plants, our knowledge on regulatory mechanisms of senescence is still poor. WRKY transcription factors have been shown to be involved in the regulation of leaf senescence. However, almost nothing is known about the upstream regulation of the senescence specific expression of WRKY factors. Therefore, we characterized proteins that bind and activate the promoter of WRKY53, which participates in leaf senescence in Arabidopsis thaliana. Surprisingly, a mitogen activated protein kinase kinase kinase (MEKK1) was identified as a DNA-binding protein. The binding motif for MEKK1 in the WRKY53 promoter could be characterized and promoter:GUS analyses revealed that this region is important for the switch of WRKY53 expression from a leaf age dependent to a systemic plant age dependent expression during bolting time. In addition to its promotor-binding activity, MEKK1 was also able to interact with the WRKY53 protein. Using bimolecular fluorescence complementation assays the complex formation of MEKK1 and WRKY53 could be localized predominately in the nucleus of Arabidopsis cells. MEKK1 could also phosphorylate WRKY53 in vitro and phosphorylation could increase DNA-binding activity of WRKY53 in vitro and transcription of a WRKY53 promoter driven reporter gene in vivo. These results suggest that MEKK1 is a bifunctional protein: it binds to the promoter of the WRKY53 gene regulating the switch from a leaf age dependent to a plant age dependent expression and it can phosopharylate WRKY53 in vitro increasing its DNA binding activity. Thus, MEKK1 might be able to take a very direct short cut in mitogen-activated protein kinase (MAPK) signalling by directly phosphorylating a transcription factor.
This paper references
10.1016/S0076-6879(02)48639-9
Gene expression and the thiol redox state.
A. Arrigo (1999)
10.1046/J.1467-7652.2003.00004.X
The molecular analysis of leaf senescence--a genomics approach.
V. Buchanan-Wollaston (2003)
10.1104/pp.103.023176
BWMK1, a Rice Mitogen-Activated Protein Kinase, Locates in the Nucleus and Mediates Pathogenesis-Related Gene Expression by Activation of a Transcription Factor1
Y. Cheong (2003)
10.1111/J.1365-313X.2005.02399.X
Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis.
V. Buchanan-Wollaston (2005)
10.1111/J.1365-3040.2005.01459.X
Senescence-specific regulation of catalases in Arabidopsis thaliana (L.) Heynh.
P. Zimmermann (2006)
Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence
V Buchanan-Wollaston (2005)
10.1038/415977a
MAP kinase signalling cascade in Arabidopsis innate immunity
T. Asai (2002)
10.1093/nar/27.1.297
Plant cis-acting regulatory DNA elements (PLACE) database: 1999
K. Higo (1999)
10.1016/J.MOLCEL.2004.06.023
The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis.
M. Teige (2004)
10.1104/PP.120.4.1015
Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis.
J. D. Miller (1999)
10.1073/pnas.252641899
NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants
Haejeong Moon (2002)
10.1016/S1360-1385(00)01600-9
The WRKY superfamily of plant transcription factors.
T. Eulgem (2000)
10.1111/J.1365-3040.2003.01158.X
Transcriptome of Arabidopsis leaf senescence
Yongfeng Guo (2004)
10.1093/PCP/PCI133
A possible role for NDPK2 in the regulation of auxin-mediated responses for plant growth and development.
G. Choi (2005)
10.1055/S-2004-815735
Senescence-related gene expression profiles of rosette leaves of Arabidopsis thaliana: leaf age versus plant age.
U. Zentgraf (2004)
10.1111/j.1364-3703.2004.00215.x
Activation of a mitogen-activated protein kinase pathway in Arabidopsis by chitin.
J. Wan (2004)
10.1007/s004250000512
Identification of a transcription factor specifically expressed at the onset of leaf senescence
K. Hinderhofer (2001)
10.1016/J.PBI.2004.07.012
WRKY transcription factors: from DNA binding towards biological function.
Bekir Ulker (2004)
10.1101/GAD.222702
Targets of AtWRKY6 regulation during plant senescence and pathogen defense.
S. Robatzek (2002)
10.1016/J.TPLANTS.2005.05.009
Emerging MAP kinase pathways in plant stress signalling.
H. Nakagami (2005)
10.1023/A:1020780022549
Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response
J. Dong (2004)
10.1105/tpc.016980
The WRKY70 Transcription Factor: A Node of Convergence for Jasmonate-Mediated and Salicylate-Mediated Signals in Plant Defense On-line version contains Web-only data.
J. Li (2004)
10.1016/S1360-1385(02)02302-6
Mitogen-activated protein kinase cascades in plants: a new nomenclature.
Kazuya Ichimura (2002)
10.1007/s11103-005-2142-1
Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis
Y. Miao (2005)
10.1126/SCIENCE.1131158
Protein Kinases Seek Close Encounters with Active Genes
J. W. Edmunds (2006)
10.1038/sj.emboj.7600737
The MAP kinase substrate MKS1 is a regulator of plant defense responses
E. Andréasson (2005)
10.1016/S0248-4900(01)01121-2
Plant MAP kinase pathways: how many and what for?
M. Wrzaczek (2001)
10.1074/JBC.M312662200
OMTK1, a Novel MAPKKK, Channels Oxidative Stress Signaling through Direct MAPK Interaction*
H. Nakagami (2004)
10.1111/J.1365-313X.2004.02160.X
Molecular events in senescing Arabidopsis leaves.
J. Lin (2004)
10.1139/G86-069
Expression of senescence in Neurospora intermedia
A. Griffiths (1986)
10.1126/SCIENCE.1127677
Activated Signal Transduction Kinases Frequently Occupy Target Genes
D. K. Pokholok (2006)
10.1007/978-3-642-79247-2_3
In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.
N. Bechtold (1998)
10.1074/jbc.M605293200
A Mitogen-activated Protein Kinase Kinase Kinase Mediates Reactive Oxygen Species Homeostasis in Arabidopsis*
H. Nakagami (2006)
10.1002/j.1460-2075.1987.tb02730.x
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
R. Jefferson (1987)
10.1104/PP.010820
Signal transduction in maize and Arabidopsis mesophyll protoplasts.
J. Sheen (2001)
10.1146/ANNUREV.CELLBIO.19.111401.091942
Regulation of MAP kinase signaling modules by scaffold proteins in mammals.
D. Morrison (2003)
10.1093/JXB/ERG267
Expression of senescence-enhanced genes in response to oxidative stress.
S. Navabpour (2003)
10.1046/J.1365-313X.2002.01482.X
Overexpression of a kinase-deficient form of the EDR1 gene enhances powdery mildew resistance and ethylene-induced senescence in Arabidopsis.
Dingzhong Tang (2002)
10.1016/S0168-9452(00)00316-2
The developmental transition to flowering represses ascorbate peroxidase activity and induces enzymatic lipid peroxidation in leaf tissue in Arabidopsis thaliana.
Ye. (2000)
10.1105/tpc.12.11.2201
A Rab1 GTPase Is Required for Transport between the Endoplasmic Reticulum and Golgi Apparatus and for Normal Golgi Movement in Plants
H. Batoko (2000)
10.1242/jcs.01346
MAP kinases as structural adaptors and enzymatic activators in transcription complexes
J. W. Edmunds (2004)
10.1074/jbc.M401099200
Dynamic Changes in the Localization of MAPK Cascade Components Controlling Pathogenesis-related (PR) Gene Expression during Innate Immunity in Parsley*
J. Lee (2004)
10.1023/A:1006201910593
A mini binary vector series for plant transformation
C. Xiang (2004)



This paper is referenced by
10.1007/978-81-322-1620-9_4
Role of Calcium/Calmodulin in Plant Stress Response and Signaling
Ritika Das (2014)
Expression and Characterization of Salt Stress Induced ABF Transcription Factors from French Bean (Phaseolus Vulgaris)
Nagesh Babu (2015)
10.1016/S1937-6448(08)01403-2
Programmed cell death in plants: new insights into redox regulation and the role of hydrogen peroxide.
Ilya Gadjev (2008)
10.1016/j.bbrc.2015.10.063
Mitogen-activated protein kinase kinase kinase (MAPKKK) 4 from rapeseed (Brassica napus L.) is a novel member inducing ROS accumulation and cell death.
L. Li (2015)
10.1007/s11103-018-0761-6
Transcriptional regulation of osmotic stress tolerance in wheat (Triticum aestivum L.)
S. H. Wani (2018)
10.1111/j.1365-313X.2008.03742.x
VH1/BRL2 receptor-like kinase interacts with vascular-specific adaptor proteins VIT and VIK to influence leaf venation.
Teresa Ceserani (2009)
10.1016/j.pbi.2009.06.008
MAPK cascade signalling networks in plant defence.
A. Pitzschke (2009)
Caractérisation d'une famille de récepteurs kinases impliqués dans le développement gamétophytique chez Arabidopsis thaliana
J. Jun. Houde (2010)
10.1104/pp.113.223412
The Single-Stranded DNA-Binding Protein WHIRLY1 Represses WRKY53 Expression and Delays Leaf Senescence in a Developmental Stage-Dependent Manner in Arabidopsis1[W][OPEN]
Ying Miao (2013)
10.1016/J.CJ.2015.02.003
Resistance to Aspergillus flavus in maize and peanut: Molecular biology, breeding, environmental stress, and future perspectives
J. Fountain (2015)
10.1093/jxb/err450
WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana
S. Besseau (2012)
10.3389/fpls.2016.00470
Hydrogen Peroxide, Signaling in Disguise during Metal Phytotoxicity
A. Cuypers (2016)
10.1007/s11105-008-0057-0
Characterization of a Novel Stress-Response Member of the MAPK Family in Malus hupehensis Rehd
Kaixuan Duan (2008)
10.1093/mp/sst026
Protein-protein interactions in the regulation of WRKY transcription factors.
Yingjun Chi (2013)
10.1186/1471-2164-15-548
The Arabidopsis Kinome: phylogeny and evolutionary insights into functional diversification
M. Zulawski (2014)
10.1186/S42483-019-0022-X
WRKY transcription factors: evolution, binding, and action
X. Chen (2019)
10.1093/dnares/dsw010
WRKY domain-encoding genes of a crop legume chickpea (Cicer arietinum): comparative analysis with Medicago truncatula WRKY family and characterization of group-III gene(s)
K. Kumar (2016)
10.1104/pp.110.171249
Hierarchy and Roles of Pathogen-Associated Molecular Pattern-Induced Responses in Nicotiana benthamiana1[W]
C. Segonzac (2011)
10.5772/33088
Abiotic Stress Responses in Plants: A Focus on the SRO Family
Rebecca S. Lamb (2012)
10.1105/tpc.111.093419
Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice[C][W]
J. Ning (2011)
10.1371/journal.pone.0120646
GhWRKY68 Reduces Resistance to Salt and Drought in Transgenic Nicotiana benthamiana
H. Jia (2015)
10.1111/j.1365-313X.2010.04233.x
A HECT E3 ubiquitin ligase negatively regulates Arabidopsis leaf senescence through degradation of the transcription factor WRKY53.
Ying Miao (2010)
10.1016/j.ecoenv.2015.01.017
Biochemical mechanisms of signaling: perspectives in plants under arsenic stress.
E. Islam (2015)
10.3389/fpls.2012.00234
Expression of ROS-responsive genes and transcription factors after metabolic formation of H2O2 in chloroplasts
S. Balazadeh (2012)
10.5423/PPJ.2010.26.2.101
Functions of MAPK Cascade Pathways in Plant Defense Signaling
Yong Hwa Cheong (2010)
10.3389/fpls.2018.00469
Nuclear Signaling of Plant MAPKs
J. Bigeard (2018)
10.1104/pp.19.00685
WRKY1 Mediates Transcriptional Regulation of Light and Nitrogen Signaling Pathways1[OPEN]
Sachin Heerah (2019)
10.1186/1471-2229-13-121
Interaction between two rice mitogen activated protein kinases and its possible role in plant defense
A. Sheikh (2012)
10.15496/PUBLIKATION-36962
Leaf Senescence in Arabidopsis thaliana and Brassica napus: From Molecular Regulation to Nutrient Remobilization Processes
L. Riester (2019)
10.1111/jipb.12513
WRKY transcription factors in plant responses to stresses.
J. Jiang (2017)
10.1093/jxb/eru054
Modelling transcriptional networks in leaf senescence.
Christopher A. Penfold (2014)
10.1111/j.1364-3703.2008.00486.x
Transcriptional analysis of the sweet orange interaction with the citrus canker pathogens Xanthomonas axonopodis pv. citri and Xanthomonas axonopodis pv. aurantifolii.
R. A. Cernadas (2008)
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