Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Plant Regeneration From Calli In Japanese Accessions Of Miscanthus

W. Takahashi, Shin-ichi Tsuruta, M. Ebina, M. Kobayashi, T. Takamizo
Published 2016 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy
Share
We evaluated the ploidy levels and tissue culture responses of 16 Japanese Miscanthus accessions, which are registered and vegetatively maintained in the National Agriculture and Food Research Organization GeneBank, Japan, to screen suitable genotypes for the molecular breeding of Miscanthus species. A ploidy analysis showed that most M. sinensis and M. sinensis var. condensatus (var. condensatus) were putative diploids, but one accession identified as M. sinensis was unexpectedly a putative tetraploid. Additionally, M. sacchariflorus and its hybrid accessions were putative tetraploids. The deoxyribonucleic acid levels in var. condensatus were significantly higher than those in the diploid M. sinensis. Of the accessions, 10, including M. sinensis and var. condensatus, could induce plant regenerable embryogenic calli from apical meristems. We selected three of these M. sinensis accessions for further experiments because their calli growth rates were faster than those of the var. condensatus accessions. Tissue culture experiments with the selected accessions indicated that the frequencies of callus and green shoot formation strongly correlated with genotype. The broad-sense heritabilities of the embryogenic callus and green shoot formation frequencies in the selected accessions were 0.75 and 0.65, respectively, indicating that the cultures’ responses were mainly controlled by genetic factors. Thus, we further selected one accession that had the highest efficiencies in callus and green shoot formation, and we observed that light during callus culturing significantly inhibited calli growth, but promoted plant regeneration from calli in the selected accession.
This paper references
Plant Regeneration from Embryogenic Calli of the Wild Sugarcane (Saccharum spontaneum L.) Clone
‘Glagah Kloet (2013)
10.1080/01621459.1943.10501811
Analysis of Variance for Percentages Based on Unequal Numbers
W. Cochran (1943)
10.1270/JSBBS.58.129
Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos
Kazuhide Rikiishi (2008)
Agrobacterium-mediated genetic transformation of Miscant hus sinensis
O-J Hwang (2014)
10.1626/pps.7.55
Screening of Regenerable Genotypes of Italian Ryegrass (Lolium multiflorum Lam.)
W. Takahashi (2004)
10.1007/s10681-013-0910-6
Diversity in ploidy levels and nuclear DNA amounts in Korean Miscanthus species
Youn-Ho Moon (2013)
10.4314/AJB.V8I7.60072
Effect of various amino acids on shoot regeneration of sugarcane ( Sacchrum officinarum L.)
S. Asad (2009)
10.1007/s11240-010-9708-6
The effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species
K. Głowacka (2010)
10.1007/BF03543353
Mapping QTLs controlling flowering date in Miscanthus sinensis Anderss.
S. G. Atienza (2003)
10.1007/s11240-008-9445-2
Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and proline
N. Nieves (2008)
A (2003c) Identification of QTLs influencing combustion quality in Miscanthus sinensis
SG Atienza (2003)
10.1023/A:1021579713832
In vivo and in vitro protective role of proline
L. Öztürk (2004)
10.5772/31889
Molecular Breeding of Grasses by Transgenic Approaches for Biofuel Production
W. Takahashi (2012)
10 : 27 – 31 Andropogoneae , Poaceae ) using AFLP and ISSR PCR
IB Holme (1997)
10.1007/978-0-387-70805-8_10
Miscanthus: Genetic resources and breeding potential to enhance bioenergy production
W. Vermerris (2008)
10.1007/978-94-007-5500-0_11
Biotechnology of Miscanthus
S. Dalton (2013)
10.3389/fpls.2013.00217
Advances in the genetic dissection of plant cell walls: tools and resources available in Miscanthus
G. Slavov (2013)
Genetic engineering of Miscanthus. In: Paterson AH (ed) Genomics of the Saccharinae, vol 11. Plant genetics and genomics: crops and models
D Engler (2013)
10.1155/2014/501016
Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B
Ok-Jin Hwang (2014)
10.2307/2987141
Statistical methods
G. W. Snedecor (1980)
10.1007/978-94-007-5500-0
Biotechnology of Neglected and Underutilized Crops
E. C. Torres (2013)
10.1111/J.1399-3054.1962.TB08052.X
A revised medium for rapid growth and bio assays with tobacco tissue cultures
T. Murashige (1962)
10.1016/S0168-9452(98)00162-9
Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism
M. Cho (1998)
10.17226/20264
Statistical genetics and plant breeding.
W. D. Hanson (1963)
Plant regeneration from embryogenic calli of the wild sugarcane (Saccharum spont aneum L.) clone 'Glagah Kloet
W Takahashi (2013)
10.1111/j.1757-1707.2011.01090.x
Establishment of an efficient in vitro culture and particle bombardment‐mediated transformation systems in Miscanthus sinensis Anderss., a potential bioenergy crop
X. Wang (2011)
10.1186/1754-6834-5-80
Bioenergy grass feedstock: current options and prospects for trait improvement using emerging genetic, genomic, and systems biology toolkits
F. A. Feltus (2012)
10.1023/A:1025041926259
Identification of QTLs associated with yield and its components in Miscanthus sinensis Anderss
S. G. Atienza (2004)
10.1007/978-1-4419-5947-8_4
The Gene Pool of Miscanthus Species and Its Improvement
E. J. Sacks (2013)
10.1038/bmt.2012.244
Investigation of the freely available easy-to-use software ‘EZR' for medical statistics
Y. Kanda (2013)
Miscanthus : European experience with a novel energy crop
I. Lewandowskia
10.1111/J.1744-697X.2010.00198.X
Plant regeneration from calli in giant reed (Arundo donax L.).
W. Takahashi (2010)
10.11461/JWARAS.57
Comparison of relative DNA content estimated using DAPI and PI-FCM in Miscanthus sinensis , Miscanthus sacchariflorus , and their hybrids
M. Nadir (2014)
Breeding approaches to improvement of yield and quality in Miscanthus grown in Europe. In: Lewandowski I, Clifton-Brown JC (eds) European Miscanthus improvement—final report september
M Deuter (2000)
Giant Miscanthus: Biomass Crop for Illinois
Rich Pryter (2007)
The cytotaxonomy of the genus Miscanthus and its phylogenic status
S Adati (1962)
10.1007/s11105-008-0070-3
Genome Size of Three Miscanthus Species
A. Rayburn (2008)
Establishment of genetic transformation system for Miscanthus sacchariflorus and obtaining of its transgenic plants
易自力 (2004)
10.1016/S1389-1723(01)80143-2
Enhanced regeneration of rice (Oryza sativa L.) embryogenic callus by light irradiation in growth phase.
C. Liu (2001)
Heritability. In: Hanson WD, Robinson HF (eds) Statistical genetics and plant breeding
WD Hanson (1963)
10.5772/1409
Transgenic Plants - Advances and Limitations
Yelda Ozden ifti (2012)
10.1046/J.1439-0523.2003.00826.X
Influencing combustion quality in Miscanthus sinensis Anderss.: identification of QTLs for calcium, phosphorus and sulphur content
S. G. Atienza (2003)
10.1111/j.1651-2227.1964.tb05158.x
Statistical Methods
G. W. Snedecor (1964)
Genetic engineering of Miscanthus. I n: Paterson AH (ed) Genomics of the Saccharinae, vol 11. Plant genetics and genomics: crops and models
D Engler (2013)
10.1007/978-1-4419-5947-8_12
Genetic Engineering of Miscanthus
Dean E. Engler (2013)
10.3732/ajb.1000258
Discovery of natural Miscanthus (Poaceae) triploid plants in sympatric populations of Miscanthus sacchariflorus and Miscanthus sinensis in southern Japan.
A. Nishiwaki (2011)
Breeding approaches to improvement of yield and quality in Miscanthus grown in Europe
M Deuter (2000)
10.1023/A:1005981300847
Embryogenic callus formation, growth and regeneration in callus and suspension cultures of Miscanthus x ogiformis Honda Giganteus' as affected by proline
I. Holme (2004)
10.1007/s00122-003-1218-z
Identification of QTLs influencing combustion quality in Miscanthus sinensis Anderss. II. Chlorine and potassium content
S. G. Atienza (2003)
10.1007/s11240-013-0419-7
Agrobacterium-mediated genetic transformation of Miscanthus sinensis
Ok-Jin Hwang (2013)
10.1093/AOB/MCF091
Characterization of a genetic resource collection for Miscanthus (Saccharinae, Andropogoneae, Poaceae) using AFLP and ISSR PCR.
T. Hodkinson (2002)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar