Online citations, reference lists, and bibliographies.

Female Versus Male: Rumex Thyrsiflorus Fingerh. Under In Vitro Conditions. Does Sex Influence In Vitro Morphogenesis?

Halina Ślesak, Katarzyna Dziedzic, Dagmara Kwolek, Magdalena Cygan, Patryk Mizia, Paweł Olejniczak, Andrzej J Joachimiak
Published 2017 · Biology
Cite This
Download PDF
Analyze on Scholarcy
Rumex thyrsiflorus Fingerh. is a dioecious plant with polymorphic sex chromosomes (XX in females, XY1Y2 in males). This species is an interesting plant for study on the structure and function of sex chromosomes, the sex ratio, and is also a valuable source of bioactive constituents. A procedure for the micropropagation of R. thyrsiflorus from hypocotyl explants, which can be useful for analyses of sex-specific morphogenetic reactions under in vitro conditions, was developed. The molecular analysis, based on genetic sex markers, allowed the sex of explants to be determined in order to create male or female in vitro cultures. Regeneration primarily occurred via indirect adventitious shoot formation as well as via somatic embryogenesis, which was confirmed by histological and scanning electron microscope (SEM) analyses. The highest frequency of explants that revealed a morphogenetic response with the highest number of regenerated adventitious shoot buds (average 11.5 shoots per explant) was obtained on a Murashige and Skoog (MS) medium supplemented with 2.27 µM thidiazuron (TDZ). Molecular analysis revealed a female-biased sex ratio under these conditions. The relationship between the sex of the explants and their morphogenetic potential was studied. The efficiency of morphogenesis varied between the two sexes and depended on the ratio of auxin and cytokinin in the medium. The germination pattern of male and female seeds under in vitro conditions was also analysed. No differences in germination time between male and female seeds and in terms of male and female seedling length were observed, which indicated that there was no competition between the sexes during seed germination when grown under uniform growth conditions.
This paper references
Somatic embryogenesis - Stress-induced remodeling of plant cell fate.
Attila Fehér (2015)
Micropropagation protocol for the hybrid sorrel Rumex tianschanicus × Rumex patientia, an energy plant. Histological, SEM and flow cytometric analyses
Halina Ślesak (2014)
Xanthine oxidase inhibitory activity of extracts prepared from Polygonaceae species.
Orsolya Orbán-Gyapai (2015)
Effects of Reduced Oxygen Tension on Germination and Seedling Growth
Sanford Marvin Siegel (1962)
Effect of sucrose concentrations on somatic embryogenesis in carnation (Dianthus caryophyllus L.)
Omid Karami (2006)
Sexual differences in shoot production and leaf dynamics in a dioecious tree , Salix sachalinen
N Ueno (2006)
What makes the sex ratio female-biased in the dioecious tree Salix sachalinensis?
Naoto T. Ueno (2007)
Environmental influence on primary sex ratio in a dioecious plant
Ivana Stehlik (2008)
New antioxidant phytopreparation from Rumex thyrsiflorus roots. III
Yu. A. Litvinenko (2008)
Sexing pollen reveals female bias in a dioecious plant.
Ivana Stehlik (2007)
A revised medium for the growth and bioassay with tobacco tissue culture
Toshio Murashige (1962)
Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
Hugo Fraga (2016)
Molecular fate of thidiazuron and its effects on auxin transport in hypocotyls tissues of Pelargonium × hortorum Bailey
Susan J. Murch (2004)
Fluorescent Banding Pattern and Species-Specific DNA Marker in Rumex thyrsiflorus Fingerh.
Aleksandra Grabowska-Joachimiak (2012)
The influence of demography and local mating environment on sex ratios in a wind-pollinated dioecious plant
Melinda Pickup (2013)
A genetic method to resolve gender complements investigations on sex ratios in Rumex acetosa.
Helena Korpelainen (2002)
Sex Ratios - Concepts and Research Methods
Peter J Mayhew (2002)
Sexual differences in shoot and leaf dynamics in the dioecious tree Salix sachalinensis
Naoto UenoN. Ueno (2006)
Reduced Rates of Sequence Evolution of Y-Linked Satellite DNA in Rumex (Polygonaceae)
Rafael Navajas-Pérez (2004)
The costs of reproduction in plants
Jose Ramón Obeso (2002)
Changes in reproductive phenology and sex ratio of Trewia nudiflora Linn . growing in sal forest of north-eastern Uttar Pradesh , India
Balmukund Chaurasia (2015)
Antiproliferative activity of polygonaceae species from the Carpathian Basin against human cancer cell lines.
Ildikó Lajter (2013)
Female-predominant sex ratios in angiosperms
David Graham Lloyd (1974)
Effect of carbohydrates on callus induction and regeneration ability in Brassica napus L
H Bogunia (2000)
Ecological context and metapopulation dynamics affect sex-ratio variation among dioecious plant populations.
David L. Field (2013)
Females make tough neighbors: sex-specific competitive effects in seedlings of a dioecious grass
Sarah M. Eppley (2005)
Sex ratio in seeds of Rumex acetosa L. as a result of sparse or abundant pollination
J Rychlewski (1975)
Effects of site conditions on sexual dimorphism and sex ratio in lowland populations of Aruncus sylvestris Kostel. (Rosaceae)
Jolanta Marciniuk (2010)
A modified CTAB DNA extraction procedure forMusa andIpomoea
Nick J. Gawel (2007)
Ecological genetics of sex ratios in plant populations
Spencer C. H. Barrett (2010)
Using LED lighting in somatic embryogenesis and micropropagation of an elite sugarcane variety and its effect on redox metabolism during acclimatization
Lais Tomaz Ferreira (2016)
Comparative analyses of sex-ratio variation in dioecious flowering plants.
David L. Field (2013)
Sex-specific responses to climate change in plants alter population sex ratio and performance
William K. Petry (2016)
An investigation on polyploidy and sex determination within the Genus Rumex
J. Żuk (2015)
Male gametophyte development and two different DNA classes of pollen grains in Rumex acetosa L., a plant with an XX/XY1Y2 sex chromosome system and a female-biased sex ratio
Magdalena Błocka-Wandas (2007)
Ivana Stehlik (2005)
The origin and evolution of the variability in a Y-specific satellite-DNA of Rumex acetosa and its relatives.
Rafael Navajas-Pérez (2006)
Genetical and ecological mechanisms regulating the sex ratio in populations of Rumex thyrsiflorus Fingerh. (Polygonaceae)
J Rychlewski (1986)
Accumulation of Y-specific satellite DNAs during the evolution of Rumex acetosa sex chromosomes
Beatrice Mariotti (2008)
Male adventitious roots of Rumex thyrsiflorus Fingerh. as a source of genetically stable micropropagated plantlets
Halina Ślesak (2015)
Seed sexing revealed female bias in two Rumex species
Dagmara Kwolek (2010)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar