Online citations, reference lists, and bibliographies.
← Back to Search

The Influential Role Of Polyamines On The In Vitro Regeneration Of Pea (Pisum Sativum L.) And Genetic Fidelity Assessment By SCoT And RAPD Markers

C. Ajithan, V. Vasudevan, D. Sathish, S. Sathish, V. Krishnan, M. Manickavasagam
Published 2019 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
A proficient and reliable in vitro plant regeneration protocol was established for pea by utilizing cotyledonary node as an explants, which excised from 3-days old imbibed seeds. The present research work explains the positive role of polyamines (PAs) accompanied by plant growth regulators (PGRs) on the multiple shoot induction and rooting in pea plant regeneration. The highest multiple shoots (65.1 shoots/explant) was attained from the cotyledonary node explant on Murashige and Skoog (MS) medium accompanied with 20 mg l−1 spermidine (SPD) along with 1.5 mg l−1 6-benzyladenine (BA). Moreover, the highest elongation of multiplied shoots (10 cm length/shoot) was noted on MS medium enriched with 1 mg l−1 of gibberellic acid (GA3). The elongated shoots produced the highest number of roots (33.66 roots/shoot) on MS medium augmented with 30 mg l−1 putrescine (PUT) along with 0.6 mg l−1 1-naphthaleneacetic acid (NAA). Rooted plants were hardened and acclimatized in the greenhouse with an existence rate of 98%. The standardized procedure with the use of PAs has enhanced the multiple shoot induction to threefold higher than the plants raised from PGRs treatments. The regenerated pea plants revealed the fivefold enhanced photosynthetic pigment, twofold enhanced antioxidant profile and the substantial growth in chloroplast count have been achieved in optimized SPD and BA supplemented MS medium compared to control plant. Start Codon Targeted polymorphism and Random Amplified Polymorphic DNA molecular markers recognized the genetic purity of the in vitro regenerated pea plants for their true type of nature. An enhanced and consistence in vitro regeneration protocol was established for pea, by the application of polyamines along with PGRs, which significantly improved the multiple shooting, shoot elongation, rooting, total chlorophyll and antioxidant profile of regenerated plants, further not hampering their genetic fidelity.
This paper references
A revised medium for the growth and bioassay with tobacco tissue culture
T. Murashige (1962)
10.1104/PP.64.5.717
Effects of Polyamines on Chlorophyll and Protein Content, Photochemical Activity, and Chloroplast Ultrastructure of Barley Leaf Discs during Senescence.
A. Cohen (1979)
10.1111/J.1365-3040.1979.TB00792.X
Interaction of polyamines and light on biochemical processes involved in leaf senescence
R. Kaur-Sawhney (1979)
10.1016/S0031-9422(00)85482-5
Radical scavenging properties of polyamines
G. Drolet (1986)
Ethylene evolution and endogenous polyamine levels during adventitious root formation in English ivy
R. Geneve (1990)
10.1007/978-3-642-74448-8_4
Pea ( Pisum sativum L.)
M. Griga (1990)
10.1071/PP9920731
Thidiazuron induces high-frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris)
Kamal A. Malik (1992)
10.1016/0168-9452(95)90010-1
Peas: Genetics, Molecular Biology and Biotechnology
E. Téoulé (1993)
10.1016/B978-0-08-040826-2.50033-3
Pea: Pisum sativum L.
G. Kalloo (1993)
10.1093/JXB/45.2.235
V-type ATPase and pyrophosphatase in endomembranes of maize roots
K. Oberbeck (1994)
10.1093/JXB/45.12.1789
Mechanisms of polyamine action during senescence responses induced by osmotic stress
A. Tiburcio (1994)
10.1016/0014-5793(94)01421-V
The second zinc atom in the matrix metalloproteinase catalytic domain is absent in the full‐length enzymes: a possible role for the C‐terminal domain
F. Willenbrock (1995)
10.1016/0014-5793(96)00379-1
First‐report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment
N. El Ghachtouli (1996)
10.1016/S0378-4290(97)00024-5
Biotechnology applied to grain legumes
P. Christou (1997)
10.1111/J.1438-8677.1997.TB00629.X
Plant Polyamines in Reproductive Activity and Response to Abiotic Stress
A. Galston (1997)
Biotechnology applied to grain legumes
CY Çiftçi (1997)
Biotechnology applied to grain legumes. Field Crop Res 53:83–97
P Christou (1997)
10.1093/JEXBOT/51.348.1237
Efficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts.
P. Durieu (2000)
10.1007/s11627-000-0035-1
The growth regulators used for bud regeneration and shoot rooting affect the competence for flowering and seed set in regenerated plants of protein peas
S. Ochatt (2000)
10.1007/s004250000439
Leaf chlorosis in oilseed rape plants (Brassica napus) grown on cadmium-polluted soil: causes and consequences for photosynthesis and growth
A. Baryla (2001)
10.1093/PCP/PCF021
Enhanced susceptibility of photosynthesis to low-temperature photoinhibition due to interruption of chill-induced increase of S-adenosylmethionine decarboxylase activity in leaves of spinach (Spinacia oleracea L.).
Lixiong He (2002)
10.1034/J.1399-3054.2002.1160302.X
Polyamine research in plants – a changing perspective
R. Kakkar (2002)
10.1016/B0-12-227050-9/00205-2
TISSUE CULTURE AND PLANT BREEDING | Clonal Forestry
D. Blakesley (2003)
10.1023/A:1025895731017
Involvement of polyamines in root development
I. Couée (2004)
10.1007/BF00195077
Effect of polyamines on stabilization of molecular complexes in thylakoid membranes of osmotically stressed oat leaves
R. Besford (2004)
10.1016/J.BMC.2004.07.028
Antioxidant activities of flavidin in different in vitro model systems.
G. K. Jayaprakasha (2004)
10.1007/BF00037762
In vitro clonal propagation of Pisum sativum L.
M. Griga (2004)
10.1023/A:1005811229988
Characterization and expression of two members of the S-adenosylmethionine decarboxylase gene family in carnation flower
M. M. Lee (2004)
10.1007/BF00232220
Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene
R. Di (2004)
10.1007/BF00029716
Efficient adventitious shoot regeneration and somatic embryogenesis in pea
S. Özcan (2004)
10.1023/A:1013343106574
Metabolism and function of polyamines in plants: recent development (new approaches)
J. Martin-Tanguy (2004)
10.1007/BF00235355
A rapid and efficient regeneration system for pea (Pisum sativum), suitable for transformation
B. Nauerby (2004)
10.1007/BF00034610
The use of RAPD technique for the identification and classification of Pisum sativum L. genotypes
P. Samec (2004)
10.1007/BF00224394
Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transfer
J. Puonti-Kaerlas (2004)
10.1007/s00299-004-0865-0
Regeneration of Pea (Pisum sativum L.) by a cyclic organogenic system
E. Tzitzikas (2004)
10.1007/BF01142315
A simple system for pea transformation
S. Bean (2005)
Efficiency of transformation of Polish cultivars of pea (Pisum sativum L.) with various regeneration capacity by using hypervirulent Agrobacterium tumefaciens strains.
T. Pniewski (2005)
10.1111/J.1365-3040.2005.01297.X
The NO world for plants: achieving balance in an open system
H. Yamasaki (2005)
10.1080/07352680590953161
Role of Polyamines in Apoptosis and Other Recent Advances in Plant Polyamines
G. Kuehn (2005)
10.1007/BF02488409
Effects of spermidine on chlorophyll content, photosynthetic activity and chloroplast ultrastructure in the dark and under light
Che-Jun Pjon (2006)
Effects of mutagenic sodium azide (NaN3) on in vitro development of four pea (Pisum sativum L.) cultivars
A. Turkan (2006)
10.1093/PCP/PCI252
Polyamines induce rapid biosynthesis of nitric oxide (NO) in Arabidopsis thaliana seedlings.
N. Tun (2006)
Use of Gamma Rays to Induce Mutations in Four Pea (Pisum sativum L.) Cultivars
C. Çiftçi (2006)
10.1007/BF02623626
Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.)
J. Jackson (2007)
10.1007/BF02632256
Evaluation of a cotyledonary node regeneration system forAgrobacterium-mediated transformation of pea (Pisum sativum L.)
M. Jordan (2007)
10.1007/s00299-007-0413-9
Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)
P. Smýkal (2007)
10.1007/s12010-008-8226-z
Spermine-Induced Morphogenesis and Effect of Partial Immersion System on the Shoot Cultures of Banana
L. Venkatachalam (2008)
10.1007/s11105-008-0060-5
Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants
B. C. Y. Collard (2008)
10.1007/s11627-008-9135-0
Leucine and spermidine enhance shoot differentiation in cucumber (Cucumis sativus L.)
A. Vasudevan (2008)
10.1007/BF02879652
Polyamines and senescence of maintenance foliage of tea,Camellia sinensis L.
R. Kakkar (2008)
10.1007/978-1-4020-6754-9_15852
Somaclonal variation
R. L. M. Pierik (2008)
10.1007/s11120-009-9441-3
Effects of exogenous putrescine on gas-exchange characteristics and chlorophyll fluorescence of NaCl-stressed cucumber seedlings
R. Zhang (2009)
Antioxidant effect of Tephrosia purpurea L. roots.
L. L. Sonawane (2010)
10.4161/psb.5.1.10291
Polyamines and abiotic stress tolerance in plants
S. Gill (2010)
10.1016/j.anaerobe.2010.06.006
Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01.
Q. Shen (2010)
10.15835/NBHA3824876
Competence versus Recalcitrance for in Vitro Regeneration
S. Ochatt (2010)
10.1007/s11033-011-1329-6
Genetic diversity in some grape varieties revealed by SCoT analyses
D. Guo (2011)
10.1007/978-1-61737-988-8_4
Immature seeds and embryos of Medicago truncatula cultured in vitro.
S. Ochatt (2011)
10.1007/s11738-011-0768-y
The effect of polyamines on the efficiency of multiplication and rooting of Withania somnifera (L.) Dunal and content of some withanolides in obtained plants
G. Sivanandhan (2011)
10.1016/j.tplants.2011.08.004
De novo shoot organogenesis: from art to science.
J. Duclercq (2011)
Micropropagation of radiated seeds of Pisum sativum
U Supe (2011)
10.1007/s11738-012-1084-x
Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. & Schult. F.) Backer ex K. Heyne using DNA-based markers
S. Singh (2012)
10.1111/j.1399-3054.2012.01623.x
Effects of salt stress on the structure and function of the photosynthetic apparatus in Cucumis sativus and its protection by exogenous putrescine.
S. Shu (2012)
10.1016/j.jplph.2012.06.006
The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated 'Williams' bananas.
A. Aremu (2012)
10.4238/2012.June.18.1
A rapid method to increase the number of F₁ plants in pea (Pisum sativum) breeding programs.
M. A. Esposito (2012)
10.1007/s00606-012-0669-6
Comparison of genetic variation and differentiation among annual Cicer species using start codon targeted (SCoT) polymorphism, DAMD-PCR, and ISSR markers
Baharak Amirmoradi (2012)
Cointry EL (2012) A rapid method to increase the number of F1 plants in pea (Pisum sativum) breeding
MA Espósito (2012)
10.1007/978-81-322-1026-9
Plant Tissue Culture: An Introductory Text
S. Bhojwani (2013)
10.1016/j.plaphy.2012.11.028
Effects of exogenous spermine on chlorophyll fluorescence, antioxidant system and ultrastructure of chloroplasts in Cucumis sativus L. under salt stress.
S. Shu (2013)
10.1007/s13562-013-0200-3
An efficient in vitro regeneration system of fieldpea (Pisum sativum L.) via. shoot organogenesis
A. Das (2013)
10.1007/978-81-322-1026-9_12
Plant tissue culture: an introductory text || somaclonal variation
S. Bhojwani (2013)
10.1007/s11240-014-0608-z
Micropropagation and validation of genetic homogeneity of Alhagi maurorum using SCoT, ISSR and RAPD markers
Tanvi Agarwal (2014)
10.1556/CRC.2014.0010
Comparative efficiency of functional gene-based markers, start codon targeted polymorphism (SCoT) and conserved DNA-derived polymorphism (CDDP) with ISSR markers for diagnostic fingerprinting in wheat (Triticum aestivum L.)
H. Hamidi (2014)
10.1007/s11240-014-0431-6
Optimized shoot regeneration for Indian soybean: the influence of exogenous polyamines
M. Arun (2014)
10.1371/journal.pone.0110855
The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress
Lipan Hu (2014)
10.1007/s10725-015-0044-z
Cytokinin profiling of long-term in vitro pea (Pisum sativum L.) shoot cultures
L. Plačková (2015)
10.1016/J.EJBT.2015.05.005
Phenolic compound production and biological activities from in vitro regenerated plants of gherkin (Cucumis anguria L.)
M. Thiruvengadam (2015)
10.3389/fpls.2015.01037
Genomic Tools in Pea Breeding Programs: Status and Perspectives
Nadim Tayeh (2015)
10.1007/s11240-017-1243-2
Assessment of the efficacy of amino acids and polyamines on regeneration of watermelon (Citrullus lanatus Thunb.) and analysis of genetic fidelity of regenerated plants by SCoT and RAPD markers
Venkatachalam Vasudevan (2017)
10.1007/s11240-018-1455-0
Phytosulfokine-alpha, an enhancer of in vitro regeneration competence in recalcitrant legumes
S. Ochatt (2018)
10.1007/s11627-018-9910-5
Efficient direct plant regeneration from immature leaf roll explants of sugarcane (Saccharum officinarum L.) using polyamines and assessment of genetic fidelity by SCoT markers
D. Sathish (2018)
10.17221/2/2014-CJGPB
Pea (Pisum sativum L.) in biology prior and after Mendel's discovery.
P. Smýkal (2018)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar