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Triazole-induced Toxicity In Developing Rare Minnow (Gobiocypris Rarus) Embryos
B. Zhu, L. Liu, Yu-Xin Gong, F. Ling, Gao-Xue Wang
Published 2014 · Biology, Medicine
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Using rare minnow (Gobiocypris rarus) at early-life stages as experimental models, the developmental toxicity of five widely used triazole fungicides (myclobutanil, fluconazole, flusilazole, triflumizole, and epoxiconazole) were investigated following exposure to 1–15 mg/L for 72 h. Meanwhile, morphological parameters (body length, body weight, and heart rate), enzyme activities (superoxide dismutase (SOD), glutathione S-transferase (GST), adenosine triphosphatase (ATPase), and acetyl cholinesterase (AChE)), and mRNA levels (hsp70, mstn, mt, apaf1, vezf1, and cyp1a) were also recorded following exposure to 0.2, 1.0, and 5.0 mg/L for 72 h. Results indicated that increased malformation and mortality, decreased body length, body weight, and heart rate provide a concentration-dependent pattern; values of 72 h LC50 (median lethal concentration) and EC50 (median effective concentration) ranged from 3 to 12 mg/L. Most importantly, the results of the present study suggest that even at the lowest concentration, 0.2 mg/L, five triazole fungicides also caused notable changes in enzyme activities and mRNA levels. Overall, the present study points out that those five triazole fungicides are highly toxic to the early development of G. rarus embryos. The information presented in this study will be helpful in better understanding the toxicity induced by triazole fungicides in fish embryos.
This paper references
Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid
S Yang (2014)
Enzyme-linked immunosorbent assay based on a monoclonal antibody for the detection of the insecticide triazophos: assay optimization and application to environmental samples.
Chizhou Liang (2007)
Atrazine affects kidney and adrenal hormones (AHs) related genes expressions of rare minnow (Gobiocypris rarus).
Lihua Yang (2010)
Developmental toxicity of 3,4-dichloroaniline on rare minnow (Gobiocypris rarus) embryos and larvae.
B. Zhu (2013)
DarT: The embryo test with the Zebrafish Danio rerio--a general model in ecotoxicology and toxicology.
R. Nagel (2002)
Chemical Hormesis: Its Historical Foundations as a Biological Hypothesis
E. Calabrese (1999)
Valiuskaite A (2004) Toxicity of pesticides to predatory mites and insects in apple-tree site under field conditions
L Raudonis (2004)
Cytotoxic effects of propiconazole and its metabolites in mouse and human hepatoma cells and primary mouse hepatocytes.
Pei-jen Chen (2008)
Effects of copper exposure on the hatching status and antioxidant defense at different developmental stages of embryos and larvae of goldfish Carassius auratus.
X. Kong (2013)
Hormetic response of cholinesterase from Daphnia magna in chronic exposure to triazophos and chlorpyrifos.
S. Li (2011)
Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na+-K+-ATPase activity in brain of rainbow trout, Oncorhynchus mykiss.
Zhihua Li (2010)
Molecular aspects of azoles-induced teratogenesis
F. Marotta (2010)
Application of dispersion-solidification liquid-liquid microextraction for the determination of triazole fungicides in environmental water samples by high-performance liquid chromatography.
C. Wang (2011)
Dosage-dependent requirement for mouse Vezf1 in vascular system development.
F. Kuhnert (2005)
Chemical class-specific gene expression changes in the zebrafish embryo after exposure to glycol ether alkoxy acids and 1,2,4-triazole antifungals.
S. Hermsen (2011)
U-shaped dose-responses in biology, toxicology, and public health.
E. Calabrese (2001)
Azole fungicides affect mammalian steroidogenesis by inhibiting sterol 14 alpha-demethylase and aromatase.
J. Zarn (2003)
Triazole induced concentration-related gene signatures in rat whole embryo culture.
J. Robinson (2012)
Lipid Peroxidation, Free Radical Scavenging Enzymes, and Glutathione Redox System in Blood of Rats Exposed to Propoxur
V. Seth (2001)
Developmental toxic effects of antifungal flusilazole administered by gavage to mice.
A. Farag (2007)
Effects of Sublethal Levels of Tributyltin Chloride in a New Toxicity Test Organism: The Chinese Rare Minnow (Gobiocypris rarus)
Z. Qun-fang (2002)
A stress-sensitive reporter predicts longevity in isogenic populations of Caenorhabditis elegans
S. Rea (2005)
Histological alternation and vitellogenin induction in adult rare minnow (Gobiocypris rarus) after exposure to ethynylestradiol and nonylphenol.
J. Zha (2007)
Fluconazole alters CYP26 gene expression in mouse embryos.
Gian Mario Tiboni (2009)
Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS.
Xiongjie Shi (2008)
Expression of two cytochrome P450 aromatase genes is regulated by endocrine disrupting chemicals in rare minnow Gobiocypris rarus juveniles.
Jingjing Wang (2010)
Differential gene expression as a toxicant-sensitive endpoint in zebrafish embryos and larvae.
Doris Voelker (2007)
Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays
M. Hamilton (1977)
CYP1A expression in liver and gills of rainbow trout (Oncorhynchus mykiss) after short-term exposure to dibenzothiophene (DBT).
M. Woźny (2010)
Bioaccumulation and biotransformation of chiral triazole fungicides in rainbow trout (Oncorhynchus mykiss).
Brad J. Konwick (2006)
Altered quantities and in vivo activities of cholinesterase from Daphnia magna in sub-lethal exposure to organophosphorus insecticides.
Hongcui Liu (2012)
Teratogenic effects of two antifungal triazoles, triadimefon and triadimenol, on Xenopus laevis development: craniofacial defects.
S. Groppelli (2005)
Comparison of the mouse Embryonic Stem cell Test, the rat Whole Embryo Culture and the Zebrafish Embryotoxicity Test as alternative methods for developmental toxicity testing of six 1,2,4-triazoles.
E. D. de Jong (2011)
The Chinese rare minnow (Gobiocypris rarus) as an in vivo model for endocrine disruption in freshwater teleosts: a full life-cycle test with diethylstilbestrol.
X. Zhong (2005)
Analysis of relative gene expression data using rea l—time quantitative PCR a nd the 2 一 ct method
J. Kenneth (2001)
Moderate exercise is an antioxidant: upregulation of antioxidant genes by training.
M. Gómez-Cabrera (2008)
Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology.
J. M. Matés (2000)
Apoptosis in zebrafish development.
M. Yamashita (2003)
Toxic effects of triazophos on rare minnow (Gobiocypris rarus) embryos and larvae.
B. Zhu (2014)
Craniofacial and axial skeletal defects induced by the fungicide triadimefon in the mouse.
E. Menegola (2005)
Triadimefon Causes Branchial Arch Malformations in Xenopus laevis Embryos (5 pp)
E. Papis (2006)
Modulation of glutathione-related antioxidant defense system of fish chronically treated by the fungicide propiconazole.
Z. Li (2010)
Single and joint action toxicity of heavy metals on early developmental stages of Chinese rare minnow (Gobiocypris rarus).
B. Zhu (2011)
Evidence for beneficial low level radiation effects and radiation hormesis.
L. Feinendegen (2005)
Fast root growth responses, root exudates, and internal detoxification as clues to the mechanisms of aluminium toxicity and resistance: a review
J. Barceló (2002)
Oxidative stress and structure-activity relationship in the zebrafish (Danio rerio) under exposure to paclobutrazol
F. Ding (2009)
Postulated pathogenic pathway in triazole fungicide induced dysmorphogenic effects.
E. Menegola (2006)
Toxicity of pesticides to predatory mites and insects in apple‐tree site under field conditions
L. Raudonis (2004)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment.
Shaoli Yang (2014)
Molecular characterization of cytochrome P450 1A and 3A and the effects of perfluorooctanoic acid on their mRNA levels in rare minnow (Gobiocypris rarus) gills.
Y. Liu (2008)
Effects of 2,4‐dichlorophenol on the expression of vitellogenin and estrogen receptor genes and physiology impairments in Chinese rare minnow (Gobiocypris rarus)
X. Zhang (2008)
Relative embryotoxicity of two classes of chemicals in a modified zebrafish embryotoxicity test and comparison with their in vivo potencies.
S. Hermsen (2011)
Factors affecting degradation rates of five triazole fungicides in two soil types: 1. Laboratory incubations
R. Bromilow (1999)
A deletion in the bovine myostatin gene causes the double–muscled phenotype in cattle
L. Grobet (1997)
Developmental toxicity of cyproconazole, an inhibitor of fungal ergosterol biosynthesis, in the rat
K. Machera (1995)
Acute ZnO nanoparticles exposure induces developmental toxicity, oxidative stress and DNA damage in embryo-larval zebrafish.
X. Zhao (2013)
Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition.
M. Soengas (1999)
Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish.
C. S. Ramsden (2013)
Evaluation of acute and developmental effects of difenoconazole via multiple stage zebrafish assays.
X. Mu (2013)
This paper is referenced by
Analysis of Sister Chromatid Exchanges and Proliferation of Human Peripheral Blood Lymphocytes Exposed to Epoxiconazole
Renata Michálková (2018)
Toxicological analysis of triadimefon on endocrine disruption and oxidative stress during rare minnow (Gobiocypris rarus) larvae development
J. Jiang (2017)
Activity and determinants of cholinesterases and paraoxonase-1 in blood of workers exposed to non-cholinesterase inhibiting pesticides.
David Lozano-Paniagua (2016)
Potential chromosome damage, cell-cycle kinetics/and apoptosis induced by epoxiconazole in bovine peripheral lymphocytes in vitro.
K. Šiviková (2018)
Extraction of triazole fungicides in environmental waters utilizing poly (ionic liquid)-functionalized magnetic adsorbent.
C. Liu (2017)
Oxidative stress and genotoxicity of nitenpyram to earthworms (Eisenia foetida).
W. Zhang (2020)
The effects of a short-term exposure to propiconazole in zebrafish (Danio rerio) embryos
F. Zhao (2020)
Fish inhabiting rice fields: Bioaccumulation, oxidative stress and neurotoxic effects after pesticides application
A. Rossi (2020)
QSAR models for the acute toxicity of 1,2,4-triazole fungicides to zebrafish (Danio rerio) embryos.
Kun Qiao (2020)
Investigation into the sub‐lethal effects of the triazole fungicide triticonazole in zebrafish (Danio rerio) embryos/larvae
C. Souders (2019)
Evaluating the effects of the tebuconazole on the earthworm, Eisenia fetida by H-1 NMR-Based untargeted metabolomics and mRNA assay.
R. Zhang (2020)
Disruption of sex-hormone levels and steroidogenic-related gene expression on Mongolia Racerunner (Eremias argus) after exposure to triadimefon and its enantiomers.
J. Li (2017)
Biomarker-based assessment of the toxicity of the antifungal clotrimazol to the microcrustacean Daphnia magna.
Madalena Vieira (2019)
Ionic liquid-based carbon nanotube coated magnetic nanoparticles as adsorbent for the magnetic solid phase extraction of triazole fungicides from environmental water
F. Chen (2016)
The developmental effect of difenoconazole on zebrafish embryos: A mechanism research.
X. Mu (2016)
Hydroxyl-containing porous organic framework coated stir bar sorption extraction combined with high performance liquid chromatography-diode array detector for analysis of triazole fungicides in grape and cabbage samples.
Yuxin Wang (2020)
Predicted no-effect concentration (PNEC) and assessment of risk for the fungicide, triadimefon based on reproductive fitness of aquatic organisms.
N. Liu (2018)
Acute exposure to the penconazole-containing fungicide Topas partially augments antioxidant potential in goldfish tissues.
V. V. Husak (2017)
Tebuconazole induced oxidative stress and histopathological alterations in adult rat heart.
Yosra Ben Othmène (2020)
Ultrasound assisted dispersive solid phase extraction of triazole fungicides by using an N-heterocyclic carbene copper complex supported on ionic liquid-modified graphene oxide as a sorbent
Zolfaghar Aladaghlo (2019)
Fungicides: An Overlooked Pesticide Class?
J. Zubrod (2019)
A comprehensive review on environmental toxicity of azole compounds to fish.
J. Bhagat (2021)
Fenbuconazole exposure impacts the development of zebrafish embryos.
Yuqiong Wu (2018)
Prenatal and infant exposure to ambient pesticides and autism spectrum disorder in children: population based case-control study
O. V. von Ehrenstein (2019)
Effects of beta-cypermethrin and myclobutanil on some enzymes and changes of biomarkers between internal tissues and saliva in reptiles (Eremias argus).
L. Chen (2019)
Development of triflumizole ionic liquids containing anions of natural origin for improving the utilization and minimizing the adverse impacts on aquatic ecosystems.
Gang Tang (2019)
Large volume of water samples introduced in dispersive liquid–liquid microextraction for the determination of 15 triazole fungicides by gas chromatography-tandem mass spectrometry
Jing Nie (2016)
The oxidative stress response of myclobutanil and cyproconazole on Tetrahymena thermophila.
Aiguo Huang (2016)
Oxidative stress, genotoxicity, biochemical and histopathological modifications induced by epoxiconazole in liver and kidney of Wistar rats
Hiba Hamdi (2019)
Synergistic potential of fenvalerate and triadimefon on endocrine disruption and oxidative stress during rare minnow embryo development
S. Wu (2018)
The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis
Y. Li (2016)
Impacts of isopyrazam exposure on the development of early-life zebrafish (Danio rerio)
Hongzhou Yao (2018)See more