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Sulfur Dioxide Induces Apoptosis Via Reactive Oxygen Species Generation In Rat Cardiomyocytes

Shuyue Li, Zhifang Xu, Jin Xia, Guohua Qin, Nan Sang
Published 2019 · Chemistry, Medicine
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Epidemiological evidence suggests that the incidence and mortality of cardiovascular diseases are closely related to sulfur dioxide (SO2). In the present study, H9C2 cells were incubated with 100 μM NaHSO3 with or without pretreatment of an antioxidant, N-acetyl-l-cysteine (NAC). The changes of apoptosis rate, mitochondrial membrane potential (MMP), ATP content, caspase-3 activity, and reactive oxygen species (ROS) were detected. Rats were inhaled 7 mg/m3 SO2 and/or intraperitoneal injected with 50 mg/kg (bw) of NAC for 30 days. RT-PCR and Western blot were used to detect the mRNA and protein levels of apoptosis-related genes. We found that the apoptosis of H9C2 cells was induced by NaHSO3, which decreased the content of MMP and ATP, and induced the expression of caspase-3. NAC can inhibit the apoptosis induced by NaHSO3 treatment. SO2 and NaHSO3 decreased the expression of Bcl-2 and the ratio of Bcl-2/Bax, increased the expression of Bax and P53 accumulation and phosphorylation, and activated caspase-9 and caspase-3. Whereas NAC can reduce the changes of apoptosis-related proteins in rat heart. Our results suggest that SO2 induces ROS-mediated P53 and caspase-dependent mitochondrial signaling pathways in H9C2 cells and rat hearts. Antioxidant therapy can reduce the adverse reactions of SO2 and lead to a decline in the cardiovascular disease induced by SO2.
This paper references
Air Pollution and Emergency Department Visits for Hypertension in Edmonton and Calgary, Canada: A Case-Crossover Study.
Robert D. Brook (2015)
Mitochondria and apoptosis.
Douglas R. Green (1998)
Air Pollution Is Associated With Ischemic Stroke via Cardiogenic Embolism
Jong-Won Chung (2017)
Functional responses of human neutrophils to sodium sulfite (Na2SO3) in vitro
Pierre B. Labbé (1998)
Delayed rectifier potassium channels are involved in SO2 derivative-induced hippocampal neuronal injury.
Guangke Li (2009)
Apoptosis: A Review of Programmed Cell Death
Susan A. Elmore (2007)
Therapeutic Potential of N-Acetylcysteine for Wound Healing, Acute Bronchiolitis, and Congenital Heart Defects.
Manaf Almatar (2016)
[Effect of air pollution on coronary heart disease mortality in Tianjin, 2001-2009: a time-series study].
De-zheng Wang (2013)
Caspase-8 regulation of TRAIL-mediated cell death.
Roslyn N. Crowder (2012)
Effects of sulfur dioxide on apoptosis-related gene expressions in lungs from rats.
Juli Bai (2005)
Sulfur Dioxide Contributes to the Cardiac and Mitochondrial Dysfunction in Rats.
Guohua Qin (2016)
Mitochondrial dysfunction is an essential step for killing of non-small cell lung carcinomas resistant to conventional treatment
Bertrand Joseph (2017)
SO2 inhalation modulates the expression of apoptosis-related genes in rat hippocampus via its derivatives in vivo
Yang No Yun (2010)
The association between air pollution and heart failure, arrhythmia, embolism, thrombosis, and other cardiovascular causes of death in a time series study.
G. van der Hoek (2001)
Association between Air Pollutants and Cardiovascular Disease Mortality in Wuhan, China
Yisi Liu (2015)
Cardiomyocyte apoptosis is related to left ventricular dysfunction and remodelling in dilated cardiomyopathy, but is not affected by growth hormone treatment.
Waltraut Ibe (2007)
Protein kinase Cdelta activation induces apoptosis in response to cardiac ischemia and reperfusion damage: a mechanism involving BAD and the mitochondria.
Christopher L Murriel (2004)
Cell Morphological Ultrastructural Changes in Various Organs from Mice Exposed by Inhalation to Sulfur Dioxide
Ziqiang Meng (2007)
[Effects of sulfur dioxide, on the proliferation and apoptosis of aorta smooth muscle cells in hypertension: experiments with rats].
Xia Zhao (2008)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M M Bradford (1976)
Mitochondrial Complex III-generated Oxidants Activate ASK1 and JNK to Induce Alveolar Epithelial Cell Death following Exposure to Particulate Matter Air Pollution*
Saul Soberanes (2009)
Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo.
Toshiyuki Miyashita (1994)
Sulfite oxidase activity of cytochrome c: Role of hydrogen peroxide
Murugesan Velayutham (2015)
Parkin Protein Deficiency Exacerbates Cardiac Injury and Reduces Survival following Myocardial Infarction*♦
Dieter A Kubli (2012)
Effects of N-Acetylcysteine on the Cardiac Remodeling Biomarkers and Major Adverse Events Following Acute Myocardial Infarction: A Randomized Clinical Trial
Azita Hajhossein Talasaz (2013)
Spinosad induces programmed cell death involves mitochondrial dysfunction and cytochrome C release in Spodoptera frugiperda Sf9 cells.
Mingjun Yang (2017)
Caspase-3 as a therapeutic target for heart failure
Bo Yang (2013)
Formation of reactive sulfite-derived free radicals by the activation of human neutrophils: an ESR study.
Kalina Ranguelova (2012)
The Impact of Autophagy on Cell Death Modalities
Stefan W Ryter (2014)
Ambient Air Pollution and Daily Outpatient Visits for Cardiac Arrhythmia in Shanghai, China
Ang Zhao (2014)
Determination of total serum sulfite by HPLC with fluorescence detection.
A J Ji (1995)
Sulfur dioxide inhalation stimulated mitochondrial biogenesis in rat brains.
Guohua Qin (2012)
Sulfur dioxide and benzo(a)pyrene trigger apoptotic and anti-apoptotic signals at different post-exposure times in mouse liver.
Guohua Qin (2015)
Long-term exposure to gaseous air pollutants and cardio-respiratory mortality in Brisbane, Australia.
Xiao Yu Wang (2009)
To die or not to die: an overview of apoptosis and its role in disease.
Steven W. Hetts (1998)
The association of daily sulfur dioxide air pollution levels with hospital admissions for cardiovascular diseases in Europe (The Aphea-II study).
Jordi Sunyer (2003)
Expression of apoptosis-related genes in livers from rats exposed to sulfur dioxide.
Juli Bai (2005)
The formation of sulfur trioxide radical anion during the prostaglandin hydroperoxidase-catalyzed oxidation of bisulfite (hydrated sulfur dioxide).
Carolyn Mottley (1982)
G-protein inactivator RGS6 mediates myocardial cell apoptosis and cardiomyopathy caused by doxorubicin.
Jianqi Yang (2013)
Ambient air pollution and out-of-hospital cardiac arrest.
Si-Hyuck Kang (2016)

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