Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Effects Of Cobalt Nanoparticles On Human T Cells In Vitro

H. Jiang, F. Liu, H. Yang, Y. Li
Published 2011 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Limited information is available on the potential risk of degradation products of metal-on-metal bearings in joint arthroplasty. The aim of this study was to investigate the cytotoxicity and genotoxicity of orthopedic-related cobalt nanoparticles on human T cells in vitro. T cells were collected using magnetic CD3 microbeads and exposed to different concentrations of cobalt nanoparticles and cobalt chloride. Cytotoxicity was evaluated by methyl thiazolyl tetrazolium and lactate dehydrogenase release assay. Cobalt nanoparticles dissolution in culture medium was determined by inductively coupled plasma-mass spectrometry. To study the probable mechanism of cobalt nanoparticles effects on T cells, superoxide dismutase, catalase, and glutathione peroxidase level was measured. Cobalt nanoparticles and cobalt ions could inhibit cell viability and enhance lactate dehydrogenase release in a concentration- and time-dependent manner (P < 0.05). The levels of cobalt ion released from cobalt nanoparticles in the culture medium were less than 40% and increased with cobalt nanoparticles concentration. Cobalt nanoparticles could induce primary DNA damage in a concentration-dependent manner, and the DNA damage caused by cobalt nanoparticles was heavier than that caused by cobalt ions. Cobalt nanoparticles exposure could significantly decrease superoxide dismutase, catalase, and glutathione peroxidase activities at subtoxic concentrations (6 μM,
This paper references
Quantitative analysis of wear and wear debris from metal-on-metal hip prostheses tested in a physiological hip joint simulator.
P. Firkins (2001)
10.4161/oxim.3.4.12860
Age associated oxidative damage in lymphocytes
N. Gautam (2010)
10.1016/j.toxlet.2009.06.851
Engineered cobalt oxide nanoparticles readily enter cells.
E. Papis (2009)
10.1007/s12011-009-8388-8
Toxic Effects of Cobalt Chloride on Hematological Factors of Common Carp (Cyprinus carpio)
S. S. Saeedi Saravi (2009)
10.2106/JBJS.D.02916
Metallosis after contemporary metal-on-metal total hip arthroplasty. Five to nine-year follow-up.
P. Korovessis (2006)
10.1093/mutage/gep027
Genotoxicity and morphological transformation induced by cobalt nanoparticles and cobalt chloride: an in vitro study in Balb/3T3 mouse fibroblasts.
J. Ponti (2009)
10.1016/j.tox.2007.12.022
Oxidative stress induced by cerium oxide nanoparticles in cultured BEAS-2B cells.
E. Park (2008)
10.2106/JBJS.E.00604
Survivorship and retrieval analysis of Sikomet metal-on-metal total hip replacements at a mean of seven years.
I. Milošev (2006)
10.1002/ANIE.200602866
Magnetic nanoparticles: synthesis, protection, functionalization, and application.
A. Lu (2007)
10.1016/J.BIOMATERIALS.2004.11.019
Induction of protein oxidation by cobalt and chromium ions in human U937 macrophages.
A. Petit (2005)
10.1021/ES062629T
Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress.
Ludwig K. Limbach (2007)
10.1289/EHP.02110S5797
Interference by toxic metal ions with DNA repair processes and cell cycle control: molecular mechanisms.
A. Hartwig (2002)
10.1016/J.ARTH.2004.09.010
Changes in metal levels and chromosome aberrations in the peripheral blood of patients after metal-on-metal hip arthroplasty.
D. Ladon (2004)
10.1302/0301-620X.85B5.13729
Immunological changes in patients with primary osteoarthritis of the hip after total joint replacement.
D. Granchi (2003)
10.1111/J.1432-1033.1974.TB03714.X
Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.
S. Marklund (1974)
10.2106/JBJS.C.00949
An unusual lymphocytic perivascular infiltration in tissues around contemporary metal-on-metal joint replacements.
A. P. Davies (2005)
Nanoparticles, free radicals and oxidative stress
M. Simkó (2011)
10.2106/JBJS.E.01077
Failed spine surgery syndrome in the life and career of John Fitzgerald Kennedy.
R. Hart (2006)
10.1088/1748-6041/4/2/025018
Dose-dependent cytotoxicity of clinically relevant cobalt nanoparticles and ions on macrophages in vitro.
Young-min Kwon (2009)
10.1002/(SICI)1097-4636(199810)42:1<103::AID-JBM13>3.0.CO;2-M
Metal wear particle characterization from metal on metal total hip replacements: transmission electron microscopy study of periprosthetic tissues and isolated particles.
P. Doorn (1998)
10.1093/mutage/gen024
Comparative genotoxicity of cobalt nanoparticles and ions on human peripheral leukocytes in vitro.
R. Colognato (2008)
10.1302/0301-620X.88B3.16748
A benign psoas mass following metal-on-metal resurfacing of the hip.
D. Boardman (2006)
Metal-on-metal versus polyethylene in hip arthroplasty: a randomized clinical trial.
J. Daniel (2004)
10.1097/00003086-199608001-00020
Distribution of cobalt chromium wear and corrosion products and biologic reactions.
K. Merritt (1996)
10.1016/S1383-5718(02)00251-6
A cross-platform public domain PC image-analysis program for the comet assay.
Krzysztof Końca (2003)
10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J
Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing
R. Tice (2000)
10.1016/j.arth.2007.09.024
Metal sensitivity as a cause of groin pain in metal-on-metal hip resurfacing.
P. Campbell (2008)
10.1016/J.BIOMATERIALS.2007.02.034
The effect of nano- and micron-sized particles of cobalt-chromium alloy on human fibroblasts in vitro.
I. Papageorgiou (2007)
10.1016/S0076-6879(84)05016-3
Catalase in vitro.
H. Aebi (1984)
Cobalt in hard metals and cobalt sulfate, gallium arsenide, indium phosphide and vanadium pentoxide.
M. Altamirano-Lozano (2006)
10.1016/s0021-9258(19)52451-6
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
10.1302/0301-620X.90B7.20213
Pseudotumours associated with metal-on-metal hip resurfacings.
H. Pandit (2008)
10.1016/J.TOXLET.2007.03.005
Gene expression in nanotoxicology research: analysis by differential display in BALB3T3 fibroblasts exposed to cobalt particles and ions.
E. Papis (2007)
10.1016/J.TOXLET.2006.11.001
Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants.
K. Pulskamp (2007)
10.1002/jbm.a.32301
In vivo production of nanosized metal wear debris formed by tribochemical reaction as confirmed by high-resolution TEM and XPS analyses.
I. Milošev (2009)
10.1302/0301-620X.91B6.21844
Circulating levels of cobalt and chromium from metal-on-metal hip replacement are associated with CD8+ T-cell lymphopenia.
A. Hart (2009)
10.1016/0014-4827(88)90265-0
A simple technique for quantitation of low levels of DNA damage in individual cells.
N. Singh (1988)
10.1126/science.179.4073.588
Selenium: Biochemical Role as a Component of Glutathione Peroxidase
J. T. Rotruck (1973)
10.1016/j.addr.2008.03.018
Magnetic nanoparticles in MR imaging and drug delivery.
C. Sun (2008)
10.1302/0301-620X.88B4.17216
The association between metal ions from hip resurfacing and reduced T-cell counts.
A. Hart (2006)
10.1136/oem.58.10.619
Update on the genotoxicity and carcinogenicity of cobalt compounds
D. Lison (2001)



This paper is referenced by
10.1039/C4NJ00772G
Catalytic generation of hydroxyl radicals by dioxygen-mediated oxidation of p-aminophenol by simple cobalt(II) ions in bicarbonate aqueous solution for use in Acid Orange 7 decolorization
W. Shi (2014)
10.1039/c3mt00167a
Effects of metal(loid)-based nanomaterials on essential element homeostasis: the central role of nanometallomics for nanotoxicology.
F. Benetti (2014)
10.1002/chem.201402289
Linking an α-tocopherol derivative to cobalt(0) nanomagnets: magnetically responsive antioxidants with superior radical trapping activity and reduced cytotoxicity.
C. Viglianisi (2014)
10.2478/10004-1254-63-2012-2213
Genotoxicity of Metal Nanoparticles: Focus on In Vivo Studies
K. Klien (2012)
10.1186/1743-8977-11-14
Low-solubility particles and a Trojan-horse type mechanism of toxicity: the case of cobalt oxide on human lung cells
R. Ortega (2013)
10.1016/j.bbrc.2017.03.045
Protective effects of Zn2+ against cobalt nanoparticles and cobalt chloride-induced cytotoxicity of RAW 264.7cells via ROS pathway.
Hai Zhu (2017)
Supporting Information 2 Extrapolation of Responses to Human Health
A. Madl (2014)
10.1007/978-1-4614-9123-1_23
Heavy Metals and White Matter Injury
Y. Li (2014)
10.1016/j.marenvres.2015.05.007
Neutral red retention time assay in determination of toxicity of nanoparticles.
Wentao Hu (2015)
Individual approach to treatment and cobalt - a new look at an old problem.
Natalia Kurhaluk (2017)
10.1002/jat.3133
Investigation on cobalt‐oxide nanoparticles cyto‐genotoxicity and inflammatory response in two types of respiratory cells
D. Cavallo (2015)
10.1016/j.nano.2015.02.006
Toxicology of wear particles of cobalt-chromium alloy metal-on-metal hip implants Part II: Importance of physicochemical properties and dose in animal and in vitro studies as a basis for risk assessment.
A. Madl (2015)
10.1002/em.21926
The role of intracellular redox imbalance in nanomaterial induced cellular damage and genotoxicity: A review
A. Kermanizadeh (2015)
10.1016/J.APCATA.2014.01.048
Catalytic activation of dioxygen to hydroxyl radical and efficient oxidation of o-aminophenol by cobalt(II) ions in bicarbonate aqueous solution
X. Li (2014)
10.3109/17435390.2012.689882
Genotoxicity of cobalt nanoparticles and ions in Drosophila
Gerard Vales (2013)
10.1007/s12011-015-0381-9
The Protective Effect of Bafilomycin A1 Against Cobalt Nanoparticle-Induced Cytotoxicity and Aseptic Inflammation in Macrophages In Vitro
S. Wang (2015)
10.3109/17435390.2013.773464
Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles
Z. Magdolénová (2014)
10.1007/s11368-019-02285-8
Effects of cobalt oxide nanomaterial on plants and soil invertebrates at different levels of biological organization
Sirine Bouguerra (2019)
10.1186/1471-2474-15-267
Metal debris concentrations in soft tissues adjacent to loosened femoral stems is higher in uncemented than cemented implants
K. Kmieć (2014)
10.3109/17435390.2013.796538
Cytotoxicity and morphological transforming potential of cobalt nanoparticles, microparticles and ions in Balb/3T3 mouse fibroblasts: an in vitro model
E. Sabbioni (2014)
10.1002/jat.3220
Zerovalent Fe, Co and Ni nanoparticle toxicity evaluated on SKOV‐3 and U87 cell lines
R. Gornati (2016)
10.1007/s00132-012-2039-z
In-vitro-Analyse des Einflusses von Metallionen auf humane Lymphozyten
S. Hagmann (2012)
10.1039/c9en00690g
In vivo evaluation of the toxic and genotoxic effects of exposure to cobalt nanoparticles using Drosophila melanogaster
Mohamed Alaraby (2020)
10.1007/s12011-016-0789-x
l-Ascorbic Acid Protected Against Extrinsic and Intrinsic Apoptosis Induced by Cobalt Nanoparticles Through ROS Attenuation
Y. Liu (2016)
10.1002/smll.201503250
Plant Polyphenol-Assisted Green Synthesis of Hollow CoPt Alloy Nanoparticles for Dual-Modality Imaging Guided Photothermal Therapy.
Xiao-rong Song (2016)
Comparative toxicological analysis of iron, cobalt and nickel nanoparticles in three different in vitro models.
S. G. Ciappellano (2014)
10.2217/nnm.14.38
Partial mitigation of gold nanoparticle interactions with human lymphocytes by surface functionalization with a 'mixed matrix'.
N. Liptrott (2014)
10.1080/1547691X.2019.1605553
Metal nanomaterials: Immune effects and implications of physicochemical properties on sensitization, elicitation, and exacerbation of allergic disease
K. A. Roach (2019)
10.1007/s12011-012-9506-6
The Effects of Cobalt on the Development, Oxidative Stress, and Apoptosis in Zebrafish Embryos
Guiquan Cai (2012)
10.4028/www.scientific.net/KEM.543.200
Biosafety Evaluation of Nanoparticles in View of Genotoxicity and Carcinogenicity Studies: A Systematic Review
H. Turkez (2013)
10.5301/hipint.5000179
Self-Reported Neurological Clinical Manifestations of Metal Toxicity in Metal-on-Metal Hip Arthroplasty
C. V. van Lingen (2014)
10.1016/j.addr.2016.08.007
In vitro toxicity assessment of oral nanocarriers.
S. G. Ciappellano (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar