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Peritoneal Cell-free DNA: An Innovative Method For Determining Acute Cell Damage In Peritoneal Membrane And For Monitoring The Recovery Process After Peritonitis

G. Virzi', S. Milan Manani, A. Brocca, V. Cantaluppi, M. Cal, S. Pastori, Ilaria Tantillo, R. Zambon, C. Crepaldi, C. Ronco
Published 2015 · Medicine

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BackgroundCell-free DNA (cfDNA) is present in the peritoneal effluent of stable peritoneal dialysis (PD) patients, but there are no data on cfDNA in PD patients with peritonitis. We investigated the variation of peritoneal cfDNA levels subsequent to peritonitis in PD patients.MethodsWe enrolled 53 PD patients: 30 without any history of systemic inflammation or peritonitis in the last 3 months (group A) and 23 with acute peritonitis (group B). CfDNA was quantified in the peritoneal effluent. Peritoneal samples on days 1, 3, 10, 30 and until day 120 from the start of peritonitis were collected for white blood cells (WBC) count and cfDNA evaluation in group B.ResultsQuantitative analysis of cfDNA showed significantly higher levels in group B on day 1, 3, 10 and 30 compared with group A (p < 0.05). A significant positive correlation was observed between cfDNA concentration and WBC on day 1 (rho = 0.89) and day 3 (rho = 0.5) (both, p < 0.05). However, no significant correlation was observed between cfDNA and WBC on days 10 and 30. In group B, peritoneal cfDNA levels tended to progressively decline during follow-up of peritonitis. From this decreasing curve, we estimated that 49 days are necessary to reach the value of 51 genome equivalents (GE)/ml (75th percentile in controls) and 63 days to reach 31 GE/ml (median).ConclusionOur results demonstrate that cfDNA increases in peritoneal effluent of PD patients with peritonitis and tends to progressively decline in step with peritonitis resolution and membrane repair process. Peritoneal cfDNA quantification could be an innovative method to determine acute damage and an inverse index of the repair process.
This paper references
10.1111/j.1749-6632.2001.tb03892.x
Circulating Nucleic Acids and Apoptosis
A. V. Lichtenstein (2001)
Acceleration of neutrophil apoptosis by glucose-containing peritoneal dialysis solutions: role of caspases.
M. Catalan (2001)
10.1016/S0009-8981(01)00665-9
About the possible origin and mechanism of circulating DNA apoptosis and active DNA release.
M. Stroun (2001)
10.1186/cc2654
Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
S. Wort (2004)
10.1093/NDT/GFH885
Peritonitis: limiting the damage.
E. Brown (2005)
10.1373/CLINCHEM.2005.058883
Cell-free plasma DNA: a marker for apoptosis during hemodialysis.
J. Atamaniuk (2006)
10.1093/NDT/GFL788
Hyperleptinaemia and chronic inflammation after peritonitis predicts poor nutritional status and mortality in patients on peritoneal dialysis.
M. Lam (2007)
10.1681/ASN.2006111292
Epithelial to mesenchymal transition and peritoneal membrane failure in peritoneal dialysis patients: pathologic significance and potential therapeutic interventions.
L. Aroeira (2007)
10.1007/s00134-007-0686-z
Association of cell-free plasma DNA with hospital mortality and organ dysfunction in intensive care unit patients
K. Saukkonen (2007)
10.1196/annals.1448.014
Cell‐Free Plasma DNA during Peritoneal Dialysis and Hemodialysis and in Patients with Chronic Kidney Disease
M. Korabecna (2008)
10.1373/clinchem.2007.101030
Cell-free plasma DNA as a predictor of outcome in severe sepsis and septic shock.
K. Saukkonen (2008)
10.1159/000235250
Plasma Cell-Free DNA Levels in Children on Peritoneal Dialysis
O. Ozkaya (2009)
10.1080/08860220902963673
Cell-Free Plasma DNA during Hemodialysis
S. Opatrná (2009)
10.1016/j.cca.2010.07.032
Cell-free DNA in the blood as a solid tumor biomarker--a critical appraisal of the literature.
K. Jung (2010)
10.1111/j.1744-9987.2009.00717.x
Cell‐free DNA in the Peritoneal Effluent of Peritoneal Dialysis Solutions
J. Pajek (2010)
10.3747/pdi.2010.00049
PERITONEAL DIALYSIS-RELATED INFECTIONS RECOMMENDATIONS: 2010 UPDATE
P. Li (2010)
10.1159/000314544
Inflammation in Peritoneal Dialysis
K. Lai (2010)
Cell-free DNA in the peritoneal effluent of peritoneal dialysis
J Pajek (2010)
10.1002/jcp.22309
Peritoneal dialysate effluent during peritonitis induces human cardiomyocyte apoptosis by regulating the expression of GATA‐4 and Bcl‐2 families
H. Wang (2011)
10.3747/pdi.2011.00600
Peritoneal Dialysis-Related Infections Recommendations: 2010 Update
B. Piraino (2011)
10.1111/j.1365-2362.2010.02435.x
Analysing cell‐free plasma DNA and SLE disease activity
J. Atamaniuk (2011)
10.1016/j.cca.2011.01.026
Origin, translocation and destination of extracellular occurring DNA--a new paradigm in genetic behaviour.
D. L. Peters (2011)
10.1093/ndt/gfr695
Apoptotic cell-free DNA promotes inflammation in haemodialysis patients.
J. Atamaniuk (2012)
10.1186/cc11466
Prognostic utility and characterization of cell-free DNA in patients with severe sepsis
Dhruva J. Dwivedi (2012)
10.1155/2012/912595
Inflammation and the Peritoneal Membrane: Causes and Impact on Structure and Function during Peritoneal Dialysis
G. Baroni (2012)
10.5301/jn.5000301
Cell-free nucleic acids as biomarkers in dialyzed patients.
M. Korabečná (2013)



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