Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

TUNEL Staining And Electron Microscopy Studies Of Apoptotic Changes In The Guinea Pig Vallate Taste Cells After Unilateral Glossopharyngeal Denervation

Y. Huang, K.-S. Lu
Published 2001 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
On the basis of our previous report that unilateral glossopharyngeal neurectomy in the guinea pig resulted in degeneration and disappearance of taste buds in ipsilateral vallate papillae (Huang and Lu 1996), it is reasonable to speculate that gustatory denervation may enhance apoptosis of taste bud cells, with taste buds decreasing in number and ultimately disappearing after neurectomy. We were therefore determined to investigate apoptosis of taste bud cells in guinea pig vallate papillae after unilateral glossopharyngeal neurectomy using both terminal deoxynuleotidyl transferase (TdT)-mediated dUTP nick-end labelling (TUNEL) at the light microscopic level and by conventional electron microscopy. A total of 34 adult guinea pigs were unilaterally glossopharyngeal-neurectomized and sacrificed at 3, 6, 12 h and 1, 3 and 7 days after surgery. The results revealed that only a very few TUNEL-positive nuclei indicating apoptosis were present in normal taste buds, but in surgically denervated papillae, they increased in number from 6 h–12 h after surgery, reached at peak on day 1 and then gradually decreased. In apoptotic cells from normal taste buds, electron microscopy revealed condensation of the chromatin against the nuclear envelope, changes in the nuclear envelope, and fragmentation of the nucleus, but the integrity of the plasma membrane and organelles was maintained. Neurectomized taste cells were also characterized by condensed and fragmentary nuclei, compactness of the cytoplasmic organelles, and the appearance of pedunculated protuberances on the cell surface. From these observations, we conclude that: (1) glossopharyngeal neurectomy enhanced apoptosis of vallate taste bud cells in guinea pig; (2) appropriate gustatory nerve innervation is an essential component for the maintenance of the taste bud, and may play a role in apoptosis of taste cells.
This paper references
10.1679/AOHC.53.103
Fine structure of the taste bud in guinea pigs. I. Cell characterization and innervation patterns.
S. Yoshie (1990)
Unilateral innervation of guinea pig vallate taste buds as determined by glossopharyngeal neurectomy and HRP neural tracing.
Y. Huang (1996)
Defining apoptosis.
D. Hockenbery (1995)
Apoptosis, oncosis, and necrosis. An overview of cell death.
G. Majno (1995)
Decreased membrane phospholipid packing and decreased cell size precede DNA cleavage in mature mouse B cell apoptosis.
D. A. Mower (1994)
10.1679/AOHC.57.267
Lamina propria macrophages involved in cell death (apoptosis) of enterocytes in the small intestine of rats.
T. Iwanaga (1994)
10.1007/s004410000294
Induction of apoptosis by colchicine in taste bud and epithelial cells of the mouse circumvallate papillae
M. Takeda (2000)
10.1111/j.1749-6632.1994.tb56830.x
Physiological Cell Death during Development and Its Relationship to Aging a
Z. Zakeri (1994)
10.1016/S0091-679X(08)61921-4
Anatomical methods in cell death.
J. Kerr (1995)
10.1007/s004410050674
Apoptosis in mouse taste buds after denervation
M. Takeda (1996)
10.1002/AR.1091680306
Fine structure of degeneration and regeneration in denervated rabbit vallate taste buds
S. Fujimoto (1970)
10.1523/JNEUROSCI.07-07-02075.1987
A sensitive period for the neural induction of taste buds
M. Hosley (1987)
Developmental neurobiology of the taste system
CM Mistretta (1991)
10.1006/DBIO.1995.0025
Apoptosis in the neuronal lineage of the mouse olfactory epithelium: regulation in vivo and in vitro.
J. Holcomb (1995)
10.1083/JCB.119.3.493
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
Y. Gavrieli (1992)
10.1038/34214
Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis
H. Sakahira (1998)
10.1016/0167-5699(90)90048-E
Cellular signalling in programmed cell death (apoptosis).
D. McConkey (1990)
10.1016/S0736-5748(96)00111-6
Mice with a targeted disruption of the neurotrophin receptor trkB lose their gustatory ganglion cells early but do develop taste buds
B. Fritzsch (1997)
10.1002/CNE.903470204
Expression of the neural cell adhesion molecule (NCAM) and polysialic acid during taste bud degeneration and regeneration
D. Smith (1994)
10.1038/bjc.1972.33
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
10.11501/3169176
Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis
坂平 英樹 (2000)
10.1002/AR.1092250308
Long‐term effects of gustatory neurectomy on fungiform papillae in the young rat
J. Ganchrow (1989)
10.1083/JCB.124.4.537
Characterization of apoptosis in cultured rat sympathetic neurons after nerve growth factor withdrawal
S. Edwards (1994)
Renewal of taste bud cells
D. Ganchrow (1992)
10.1159/000145555
Trophic specificity of the gustatory fibers upon taste bud regeneration.
F. State (1982)
10.1007/s004410000193
The morphology of apoptosis
G. Häcker (2000)
10.1002/(SICI)1096-9861(19961223)376:4<587::AID-CNE7>3.0.CO;2-Y
Differential expression of brain‐derived neurotrophic factor and neurotrophin 3 mRNA in lingual papillae and taste buds indicates roles in gustatory and somatosensory innervation
C. Nosrat (1996)
10.1002/PATH.1711420112
Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis
A. Wyllie (1984)
10.1002/PATH.1711530404
Apoptosis: Cell death in tissue regulation
A. Wyllie (1987)
10.1002/CNE.903600413
Brain‐derived neurotrophic factor mRNA is expressed in the developing taste bud‐bearing tongue papillae of rat
C. Nosrat (1995)
10.1016/S0074-7696(08)62312-8
Cell death: the significance of apoptosis.
A. Wyllie (1980)
Embryonic taste buds develop in the absence of innervation.
L. Barlow (1996)
10.1002/PATH.1711700102
In situ end‐labelling detects DNA strand breaks in apoptosis and other physiological and pathological states
B. Ansari (1993)
Lingual deficits in BDNF and NT3 mutant mice leading to gustatory and somatosensory disturbances, respectively.
C. Nosrat (1997)
10.1083/JCB.123.5.1207
Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor
T. Deckwerth (1993)
10.1002/AJA.1001720104
Structure of taste buds in foliate papillae of the rhesus monkey, Macaca mulatta.
A. Farbman (1985)
tomical methods in cell death
Kerr JFR (1995)



This paper is referenced by
10.1679/AOHC.72.91
Histochemical changes and apoptosis in degenerating taste buds of the rat circumvallate papilla.
Y. Ichimori (2009)
10.1679/AOHC.71.59
Cell-type specific occurrence of apoptosis in taste buds of the rat circumvallate papilla.
K. Ueda (2008)
10.1186/s12870-020-02610-1
Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence
Sheng-long Yang (2020)
Anoxia / reoxygenation induced cell death in crucian carp brain
L. Yuen (2010)
10.1523/JNEUROSCI.4167-11.2012
Mechanisms of Taste Bud Cell Loss after Head and Neck Irradiation
H. M. Nguyen (2012)
10.1186/1471-2202-12-34
Accelerated turnover of taste bud cells in mice deficient for the cyclin-dependent kinase inhibitor p27Kip1
T. A. Harrison (2010)
10.1097/01.ASN.0000061779.70530.06
Endothelial cell apoptosis during glomerular capillary lumen formation in vivo.
W. Fierlbeck (2003)
10.1007/s10735-009-9240-3
Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis
L. M. McClusky (2009)
10.1002/JEMT.10396
Immunoelectron microscopic studies on protein gene product 9.5 and calcitonin gene‐related peptide in vallate taste cells and related nerves in the guinea pig
Y. Huang (2003)
10.3389/fchem.2020.00476
Novel Frog Skin-Derived Peptide Dermaseptin-PP for Lung Cancer Treatment: In vitro/vivo Evaluation and Anti-tumor Mechanisms Study
Ziyi Dong (2020)
10.1093/CHEMSE/BJJ030
Characterization and long-term maintenance of rat taste cells in culture.
H. Ozdener (2006)
10.1096/fj.07-8415com
The gustatory pathway is involved in CD36‐mediated orosensory perception of long‐chain fatty acids in the mouse
D. Gaillard (2008)
10.1002/cne.20064
Taste bud cell dynamics during normal and sodium‐restricted development
S. J. Hendricks (2004)
10.1371/journal.pbio.2001959
Inflammation arising from obesity reduces taste bud abundance and inhibits renewal
A. Kaufman (2018)
10.1002/cne.20670
Effects of glossopharyngeal nerve section on the expression of neurotrophins and their receptors in lingual taste buds of adult mice
C. Yee (2005)
10.1093/chemse/bjt059
Taste bud homeostasis in health, disease, and aging.
P. Feng (2014)
Mosaic Analysis with Double Markers (MADM) as a Method to Map Cell Fates in Adult Mouse Taste Buds.
Preston D. Moore (2010)
10.1186/1471-2202-11-72
Lipopolysaccharide-induced inflammation attenuates taste progenitor cell proliferation and shortens the life span of taste bud cells
Zachary J. Cohn (2009)
10.1007/s12272-009-2114-0
Antihypertensive role of glossopharyngeal nerve stimulation by nifedipine using as calcium channel blocking agent in hypertension: An experimental study
M. Aydın (2009)
10.1093/chemse/bjz076
The Mystery of "Metal Mouth" in Chemotherapy.
A. Reith (2020)
Semantic Scholar Logo Some data provided by SemanticScholar