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Use Of Thiol-terminal Silanes And Heterobifunctional Crosslinkers For Immobilization Of Antibodies On Silica Surfaces.

S. Bhatia, L. Shriver-Lake, K. Prior, J. Georger, J. Calvert, R. Bredehorst, F. Ligler
Published 1989 · Chemistry, Medicine

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A procedure for covalent immobilization of functional proteins on silica substrates was developed using thiol-terminal silanes and heterobifunctional cross-linkers. Using this procedure, a high density of functional antibodies was bound to glass cover slips and silica fibers. The amount of anti-IgG antibody immobilized was determined to be in the range of 0.66 to 0.96 ng/mm2 using radiolabeled antibody. The relative amount of IgG antigen bound by the immobilized antibody (0.37 to 0.55 mol antigen/mol antibody) was three to five times greater than other investigators have reported. In addition, the amount of protein nonspecifically adsorbed to the antibody-coated surface was further reduced by the addition of blocking agents so that nonspecific adsorption of protein antigens represented only 2-6% of the total antigen binding. With this low background, IgG antigen binding could be measured at levels as low as 150 fmol when an antigen concentration of 3 pmol/ml was applied. The process for antibody immobilization is straightforward, easy to perform, and adaptable for modifying mass quantities of biosensor components.
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This paper is referenced by
Biomimetic surface coatings from modular amphiphilic proteins
J. Harden (2010)
10.1007/978-1-4615-4717-4_70
Sensitivity of protein C immunosensor with and without human serum albumin.
H. I. Balcer (1999)
10.1128/JCM.33.2.336-341.1995
Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor.
L. Cao (1995)
10.1016/S0956-5663(97)87057-1
Evaluation of a fiber optic immunosensor for quantitating cocaine in coca leaf extracts.
A. Toppozada (1997)
10.3389/fmicb.2017.00321
Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors
K. Bene (2017)
10.1016/S0956-5663(99)00044-5
Development of sensors for direct detection of organophosphates. Part I: Immobilization, characterization and stabilization of acetylcholinesterase and organophosphate hydrolase on silica supports.
A. Singh (1999)
10.1093/clinchem/45.9.1683
Development of Kinetic Ligand-binding Assays Using a Fiber Optic Sensor.
Smith (1999)
10.1088/1361-6528/aabc4d
Magnetically-focusing biochip structures for high-speed active biosensing with improved selectivity.
Haneul Yoo (2018)
10.1146/ANNUREV.BIOENG.2.1.227
Microengineering of cellular interactions.
A. Folch (2000)
10.1016/S0956-5663(99)00052-4
Array biosensor for detection of biohazards.
C. A. Rowe-Taitt (2000)
10.1007/BF02916421
Preparation of immobilized proteins covalently coupled through silane coupling agents to inorganic supports
H. Weetall (1993)
10.1109/IEMBS.1989.96250
Sensors on surfaces
P.E. Schoen (1989)
10.1016/S0925-4005(97)80248-1
Mechanisms of interaction in acoustic plate mode immunosensors
F. Bender (1997)
10.1039/C5NJ00033E
Gold nanoparticle–mesoporous silica sheet composites with enhanced antibody adsorption capacity
Kazuma Nakanishi (2015)
10.1201/9780203913338-9
Surface Properties of Polymeric Biomaterials and Their Modification for Tissue Engineering Applications
Ays¸en Tezcaner and Vasif Hasirci (2003)
10.1016/J.TSF.2009.10.110
In-situ protein adsorption study on biofunctionalized surfaces using spectroscopic ellipsometry
Dileep Goyal (2010)
10.1016/J.TSF.2006.04.006
Static solvent contact angle measurements, surface free energy and wettability determination of various self-assembled monolayers on silicon dioxide
D. Janssen (2006)
10.1007/0-387-29540-2_16
Preliminary study of fiber optic multi-cardiac-marker biosensing system for rapid coronary heart disease diagnosis and prognosis.
Liang Tang (2006)
10.4315/0362-028X-66.10.1851
Detection of staphylococcal enterotoxin B in spiked food samples.
L. Shriver-Lake (2003)
10.1016/j.bios.2010.04.044
Compact quantitative optic fiber-based immunoarray biosensor for rapid detection of small analytes.
F. Long (2010)
10.1016/S0142-9612(00)00343-4
Long-term stability of grafted polyethylene glycol surfaces for use with microstamped substrates in neuronal cell culture.
D. Branch (2001)
10.1021/AC00059A025
Optical determination of surface density in oriented metalloprotein nanostructures
Hun Gi. Hong (1993)
10.3390/ijms17111854
Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins
A. P. Gunning (2016)
10.1016/S0956-5663(96)00074-7
Effectiveness of protein A for antibody immobilization for a fiber optic biosensor.
G. P. Anderson (1997)
10.1114/1.1433487
Assay Procedure Optimization of a Rapid, Reusable Protein C Immunosensor for Physiological Samples
H. I. Balcer (2004)
A Study of Cultured Cells on a Nanowire-based Reverse Transfection Device
K. M. Beckwith (2011)
10.1016/J.APSUSC.2011.01.097
X-ray photoelectron spectroscopic analysis of the surface chemistry of silica nanowires
M. Beaux (2011)
10.1016/j.bios.2010.10.018
Ultrasensitive quantum dots-based DNA detection and hybridization kinetics analysis with evanescent wave biosensing platform.
F. Long (2011)
10.1016/0956-5663(96)82333-5
Biosensors for chemical and biological agents of defence interest.
B. Paddle (1996)
10.1002/CJCE.20038
Observations and analysis of electrokinetically driven particle trapping in planar microelectrode arrays
M. Tomkins (2008)
10.1016/J.JIM.2003.11.008
Colorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement.
Ru-Qiang Liang (2004)
10.1109/IEMBS.2009.5335200
Exploiting Sub-threshold and above-threshold characteristics in a silver-enhanced gold nanoparticle based biochip
Yang Liu (2009)
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