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Tumor Necrosis Factor Induced Oxidative Stress In Isolated Mouse Hepatocytes.

G. Adamson, R. Billings
Published 1992 · Biology, Medicine

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Tumor necrosis factor alpha (TNF alpha) is a macrophage-derived cytokine which participates in homeostatic tissue repair. It is also a potentially useful antitumor agent. Liver toxicity, however, limits TNF alpha's clinical utility and suggests that it may play a role in liver toxicity of various etiologies. To determine the direct effects of TNF alpha on hepatocytes, in the absence of infiltrating leukocytes and other inflammatory mediators, an isolated mouse hepatocyte model has been used in the present study. Hepatocytes exposed to recombinant human TNF alpha (1-10 micrograms/ml) exhibited intracellular GSH depletion and GSSG efflux during the first 2 hr of exposure, but no cytotoxicity was observed. However, TNF alpha was toxic to hepatocytes pretreated with 1,3-bis(chloroethyl)-1-nitrosourea to inhibit GSSG-reductase activity. Furthermore, these cells exhibited a greater efflux of GSSG upon exposure to TNF alpha. TNF alpha also caused a marked decrease in cellular ATP concentrations, which occurred after initiation of effects on the glutathione pool. These findings indicate that high concentrations of TNF alpha induce an oxidant stress in isolated hepatocytes. The antioxidants mannitol and benzoate, as well as the iron chelator deferoxamine, reduced the extent of TNF alpha-induced oxidant effects in hepatocytes, which indicates that the oxidant stress may involve hydroxyl radical generation. Hepatocytes treated with ruthenium red or fructose were less susceptible to TNF alpha-induced ATP depletion, which suggests that mitochondrial calcium cycling may be involved in disruption to energy metabolism.
This paper references
10.1042/BJ1400143
The inhibition of mitochondrial calcium transport by lanthanides and ruthenium red.
K. C. Reed (1974)
10.1111/J.1432-1033.1984.TB07938.X
Oxidation of glutathione during hydroperoxide metabolism. A study using isolated hepatocytes and the glutathione reductase inhibitor 1,3-bis(2-chloroethyl)-1-nitrosourea.
L. Eklöw (1984)
10.1126/SCIENCE.2413547
Tumor necrosis factor (TNF).
L. Old (1985)
10.1126/SCIENCE.3764421
Shock and tissue injury induced by recombinant human cachectin.
K. Tracey (1986)
10.1016/0041-008X(86)90052-9
Potential role of activated macrophages in acetaminophen hepatotoxicity. II. Mechanism of macrophage accumulation and activation.
D. Laskin (1986)
10.1016/0006-291X(86)91133-2
Human hepatocytes metabolizing ethanol generate a non-polar chemotactic factor for human neutrophils.
F. Roll (1986)
Tumour cell killing by tumour necrosis factor: inhibition by anaerobic conditions, free-radical scavengers and inhibitors of arachidonate metabolism.
N. Matthews (1987)
Toxicity of tumor necrosis factor is synergistic with gamma-interferon and can be reduced with cyclooxygenase inhibitors.
J. Talmadge (1987)
10.1016/0160-5402(87)90026-X
Isolation of hepatocytes from postnatal mice.
A. Harman (1987)
10.1038/330662A0
Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia
K. Tracey (1987)
10.1016/0003-9861(88)90072-0
Peroxidation-dependent and peroxidation-independent mechanisms by which acetaminophen kills cultured rat hepatocytes.
J. Farber (1988)
10.1016/0009-2797(88)90111-1
Neutrophil-derived oxidants as mediators of chemical activation in bone marrow.
L. Twerdok (1988)
10.1021/TX00001A013
Metabolism of procainamide to a hydroxylamine by human neutrophils and mononuclear leukocytes.
J. Uetrecht (1988)
Effect of extracellular Ca++ omission on isolated hepatocytes. II. Loss of mitochondrial membrane potential and protection by inhibitors of uniport Ca++ transduction.
Claude E. Thomas (1988)
10.1016/0006-2952(88)90114-1
Comparison of the susceptibility of hepatocytes from postnatal and adult mice to hepatotoxins.
G. J. Adamson (1988)
10.1016/0006-2952(88)90248-1
Leukotriene-mediated liver injury.
G. Tiegs (1988)
10.1126/science.3263703
Induction of manganous superoxide dismutase by tumor necrosis factor: possible protective mechanism
G. Wong (1988)
10.1016/0014-5793(89)80454-5
Inhibition of target cell mitochondrial electron transfer by tumor necrosis factor
J. Lancaster (1989)
Tumour necrosis factor: clinical relevance.
A. Jones (1989)
10.1016/0006-2952(89)90630-8
A role for the glutathione peroxidase/reductase enzyme system in the protection from paracetamol toxicity in isolated mouse hepatocytes.
G. J. Adamson (1989)
10.1001/ARCHSURG.1989.01410040055012
Recombinant human tumor necrosis factor produces hemodynamic changes characteristic of sepsis and endotoxemia.
W. Schirmer (1989)
Calcium-independent pathway of tumor necrosis factor-mediated lysis of target cells.
Y. Hasegawa (1989)
The role of oxidant injury in tumor cell sensitivity to recombinant human tumor necrosis factor in vivo. Implications for mechanisms of action.
R. J. Zimmerman (1989)
Intracellular hydroxyl radical production induced by recombinant human tumor necrosis factor and its implication in the killing of tumor cells in vitro.
N. Yamauchi (1989)
10.1016/0092-8674(89)90944-6
Manganous superoxide dismutase is essential for cellular resistance to cytotoxicity of tumor necrosis factor
G. Wong (1989)
10.1080/00039896.1989.9935901
Intracellular Ca2+ and cytotoxicity.
W. S. Lynn (1989)
Cells of the hepatic sinusoid
T. Daemen (1989)
10.1097/00000658-199010000-00009
Multiple cytokines are required to induce hepatocyte nitric oxide production and inhibit total protein synthesis.
R. Curran (1990)
10.1096/fasebj.4.14.2172061
Cytotoxic mechanism of tumor necrosis factor‐α
J. Larrick (1990)
10.1001/ARCHSURG.1991.01410260074010
Effect of endogenous nitric oxide on mitochondrial respiration of rat hepatocytes in vitro and in vivo.
J. Stadler (1991)
10.1016/0041-008X(91)90087-U
The effects of fructose on adenosine triphosphate depletion following mitochondrial dysfunction and lethal cell injury in isolated rat hepatocytes.
J. R. Cannon (1991)
10.1016/0006-2952(91)90161-W
Time course of IL-6 and TNFα release during endotoxin-induced endotoxin tolerance in rats
S. Flohé (1991)
10.1016/0006-2952(91)90298-J
Endotoxin-inducible cytotoxicity in liver cell cultures--I.
T. Hartung (1991)



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X. Zhong (2017)
10.1139/cjpp-2016-0188
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Bentolhoda Ghiassy (2017)
10.5152/EURJRHEUM.2015.0031
Oxidative stress and related factors in patients with ankylosing spondylitis.
D. Solmaz (2016)
10.1080/02772248.2015.1041956
Cytogenotoxicity and histopathological assessment of Lekki Lagoon and Ogun River in Synodontis clarias (Linnaeus, 1758)
C. Alimba (2015)
Application of Systems Biology Analysis to Hepatic Injury Following Hemorrhagic Shock
Charles E. Determan (2014)
10.1002/jmv.23932
Circulating pro‐ and anti‐angiogenic mediators in patients infected with hepatitis C at different stages of hepatocellular carcinoma
R. Talaat (2014)
OXIDATIVE STRESS AND CHRONIC ALCOHOL LIVER DISEASE: THE CURRENT PERSPECTIVES
M. Singh (2014)
10.1016/j.bjid.2013.02.009
Association between insulin resistance and sustained virologic response in hepatitis C treatment, genotypes 1 versus 2 and 3: systematic literature review and meta-analysis.
M. Laurito (2013)
10.3109/08923973.2013.775589
The role of infliximab on paracetamol-induced hepatotoxicity in rats
I. Ferah (2013)
10.1155/2013/870628
Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats
I. Y. Iskusnykh (2013)
10.5897/AJB2013.12014
Genotoxicity and mutagenicity of solid waste leachates: A review
A. Bakare (2013)
10.1074/jbc.M113.488387
Proteome Variations in Pancreatic Stellate Cells upon Stimulation with Proinflammatory Factors*
Aseel J. Marzoq (2013)
Proinflammatory Factors Cells upon Stimulation with Proteome Variations in Pancreatic Stellate Genomics and Proteomics :
Aseel J. Marzoq (2013)
10.1016/j.freeradbiomed.2012.12.003
Oxidative state of the liver of rats with adjuvant-induced arthritis.
J. F. Comar (2013)
10.1016/j.ijbiomac.2013.09.026
Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells.
Zi-Jie Su (2013)
10.7763/IJBBB.2012.V2.104
Relationship of Oxidative Stress with HIV Disease Progression in HIV/HCV Co-infected and HIV Mono-infected Adults in Miami.
D. Shin (2012)
10.1016/j.freeradbiomed.2012.03.019
HIV-1, reactive oxygen species, and vascular complications.
K. M. Porter (2012)
10.5812/hepatmon.837
Oxidative Stress and Benefits of Antioxidant Agents in Acute and Chronic Hepatitis
M. Eşrefoğlu (2012)
10.5923/J.RE.20120204.04
Liver and Kidney Dysfunction in Wistar Rats Exposed to Municipal Landfill Leachate
C. Alimba (2012)
10.1007/s13346-012-0115-x
Tumor-initiating stem-like cells and drug resistance: carcinogenesis through Toll-like receptors, environmental factors, and virus
K. Machida (2012)
10.1002/9781118229415.CH19
Antioxidants and Human Health
F. Shahidi (2012)
10.3390/toxins4111139
Apoptosis Induction by OTA and TNF-α in Cultured Primary Rat Hepatocytes and Prevention by Silibinin
E. Essid (2012)
10.2165/00003495-200868130-00001
HMG-CoA Reductase Inhibitors
P. Kodaman (2012)
10.1142/S0192415X11008993
Hepatoprotective effect of Crossostephium chinensis (L.) Makino in rats.
Tien-Ning Chang (2011)
Beta-catenin in liver: A matter of life and death
Kari Nejak-Bowen (2010)
10.1007/978-3-540-93842-2_14
Free Radicals, Reactive Oxygen Species, Oxidative and Endoplasmic Reticulum Stress
Henryk Dancygier (2010)
10.1111/j.1549-8719.2010.00044.x
Carbon Monoxide has Antioxidative Properties in the Liver Involving p38 MAP Kinase Pathway in a Murine Model of Systemic Inflammation
Juergen Brugger (2010)
10.1155/2010/518674
TLRs, Alcohol, HCV, and Tumorigenesis
K. Machida (2010)
10.1007/s12072-009-9158-6
Role of free radicals in liver diseases
P. Muriel (2009)
10.1201/9781420091311.CH13
Antioxidants, Polyphenols, and Adipose Inflammation
F. Shahidi (2009)
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