← Back to Search
Studies On Membrane Fusion. III. The Role Of Calcium-induced Phase Changes.
D. Papahadjopoulos, W. Vail, C. Newton, S. Nir, K. Jacobson, G. Poste, R. Lazo
Published 1977 · Chemistry, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
The interaction of phosphatidylserine vesicles with Ca2+ and Mg2+ has been examined by several techniques to study the mechanism of membrane fusion. Data are presented on the effects of Ca2+ and Mg2+ on vesicle permeability, thermotropic phase transitions and morphology determined by differential scanning calorimetry, X-ray diffraction, and freeze-fracture electron microscopy. These data are discussed in relation to information concerning Ca2+ binding, charge neutralization, molecular packing, vesicle aggregation, phase transitions, phase separations and vesicle fusion. The results indicate that at Ca2+ concentrations of 1.0-2.0 mM, a highly cooperative phenomenon occurs which results in increased vesicle permeability, aggregation and fusion of the vesicles. Under these conditions the hydrocarbon chains of the lipid bilayers undergo a phase change from a fluid to a crystalline state. The aggregation of vesicles that is observed during fusion is not sufficient range of 2.0-5.0 mM induces aggregation of phosphatidylserine vesicles but no significant fusion nor a phase change. From the effect of variations in pH, temperature, Ca2+ and Mg2+ concentration on the fusion of vesicles, it is concluded that the key event leading to vesicle membrane fusion is the isothermic phase change induced by the bivalent metals. It is proposed that this phase change induces a transient destabilization of the bilayer membranes that become susceptible to fusion at domain boundaries.
This paper references
Studies on membrane fusion. II. Induction of fusion in pure phospholipid membranes by calcium ions and other divalent metals.
D. Papahadjopoulos (1976)
Calorimetric and freeze-etch study of the influence of Mg2+ on the thermotropic behaviour of phosphatidylglycerol.
P. Ververgaert (1975)
Fusion of fatty acid containing lecithin vesicles.
H. Kantor (1975)
Mechanisms of virus-induced cell fusion.
G. Poste (1972)
Biophysical properties of phospholipids. II. Permeability of phosphatidylserine liquid crystals to univalent ions.
D. Papahadjopoulos (1966)
Inside-outside transitions of phospholipids in vesicle membranes.
R. Kornberg (1971)
Phospholipid model membranes. 3. Antagonistic effects of Ca2+ and local anesthetics on the permeability of phosphatidylserine vesicles.
D. Papahadjopoulos (1970)
Phospholipid exchange between bilayer membrane vesicles.
F. Martín (1976)
Adsorption and desorption studies of phospholipid at the air-water interface.
T. Seimiya (1972)
Physical studies of phospholipids. XI. Ca2+ binding to monolayers of phosphatidylserine and phosphatidylinositol.
H. Hauser (1969)
Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies.
B. D. Ladbrooke (1969)
Lateral phase separations and perpendicular transport in membranes.
S. Wu (1973)
Mechanism of ion escape from phosphatidylcholine and phosphatidylserine single bilayer vesicles.
H. Hauser (1973)
Diffusion of univalent ions across the lamellae of swollen phospholipids.
A. Bangham (1965)
Permeability properties of phospholipid membranes: effect of cholesterol and temperature.
D. Papahadjopoulos (1972)
Divalent Ions and the Surface Potential of Charged Phospholipid Membranes
S. G. McLaughlin (1971)
Surface properties of acidic phospholipids: interaction of monolayers and hydrated liquid crystals with uni- and bi-valent metal ions.
D. Papahadjopoulos (1968)
Studies on membrane fusion. I. Interactions of pure phospholipid membranes and the effect of myristic acid, lysolecithin, proteins and dimethylsulfoxide.
D. Papahadjopoulos (1976)
Effects of structural defects in sonicated phospholipid vesicles on fusion and ion permeability
R. Lawaczeck (1975)
Lateral compressibility and penetration into phospholipid monolayers and bilayer membranes
M. Phillips (1975)
Effects of phospholipid acyl chain fluidity, phase transitions, and cholesterol on (Na+ + K+)-stimulated adenosine triphosphatase.
H. Kimelberg (1974)
Exchange of various phospholipids and of cholesterol between liposomes in the presence of highly purified phospholipid exchange protein.
C. Ehnholm (1973)
Phase transitions in phospholipid vesicles. Fluorescence polarization and permeability measurements concerning the effect of temperature and cholesterol.
D. Papahadjopoulos (1973)
Fusion of dimyristoyllecithin vesicles as studied by proton magnetic resonance spectroscopy.
J. Prestegard (1974)
Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations.
K. Jacobson (1975)
Membrane fusion and molecular segregation in phospholipid vesicles.
D. Papahadjopoulos (1974)
Cardiolipin forms hexagonal structures with divalent cations.
R. Rand (1972)
Effect of a phase transition on the binding of 1-anilino-8-naphthalenesulfonate to phospholipid membranes.
K. Jacobson (1976)
The influence of pH, Ca2+ and protein on the thermotropic behaviour of the negatively charged phospholipid, phosphatidylglycerol.
A. Verkleij (1974)
Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals.
D. Papahadjopoulos (1967)
Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles.
D. Papahadjopoulos (1975)
Hydrolysis of phosphatidylcholine liposomes by pancreatic phospholipase A2 at the transition temperature
J.A.F. Op den Kamp (1974)
Asymmetric exchange of vesicle phospholipids catalyzed by the phosphatidylcholine exhange protein. Measurement of inside--outside transitions.
J. Rothman (1975)
Stability of Asymmetric Phospholipid Membranes
D. Papahadjopoulos (1969)
Calcium and the Secretory Process
R. Rubin (1974)
Biophysical properties of phospholipids. I. Interaction of phosphatidylserine monolayers with metal ions.
A. Bangham (1966)
Effect of phase transition on the kinetics of dye transport in phospholipid bilater structures.
T. Tsong (1975)
This paper is referenced by
Membrane-Membrane Interactions via Intermediates in Lamellar-to-Inverted Hexagonal Phase Transitions
D. P. Siegel (1987)
Hydrophobizing effect of cations on acidic phospholipid membranes
M. Mirza (1998)
Asymmetry requirement for Ca2+ induced fusion of phosphatidylcholine-phosphatidic acid vesicles.
M. Liao (1979)
Influence of calcium on phosphatidylglycerol. Two separate lamellar structures.
K. Harlos (1980)
Abortive infection with Sindbis virus of a Chinese hamster ovary cell mutant defective in phosphatidylserine and phosphatidylethanolamine biosynthesis.
O. Kuge (1989)
Fast, Ca2+-dependent exocytosis at nerve terminals: Shortcomings of SNARE-based models
S. D. Meriney (2014)
The water permeability of Arabidopsis plasma membrane is regulated by divalent cations and pH.
P. Gerbeau (2002)
Effect of calcium(II) ions on the radiation-induced free-radical fragmentation of dimyristoyl phosphatidylglycerol as a constituent of model membranes
O. Shadyro (2000)
Modulation of membrane fusion by membrane fluidity: temperature dependence of divalent cation induced fusion of phosphatidylserine vesicles.
J. Wilschut (1985)
Understanding cochleate formation: insights into structural development.
Kalpa Nagarsekar (2016)
Chapter 10 Membrane Fusion Intermediates
P. Yeagle (1997)
Liposome-mediated delivery of DNA to carrot protoplasts
B. Matthews (2004)
Short and long-range forces involved in cation-induced aggregation of chromaffin granule membranes.
D. Haynes (1979)
Cytochemical analysis of mammalian sperm membranes.
F. Sinowatz (1989)
Kinetics of Ca2+-induced fusion of cardiolipin-phosphatidylcholine vesicles: correlation between vesicle aggregation, bilayer destabilization, and fusion.
J. Wilschut (1985)
Synergistic effect of phosphatidylserine with γ-aminobutyric acid in antagonizing the isoniazid-induced convulsions in mice
G. Toffano (2004)
Membrane fusion: Kinetics and mechanisms
J. Bentz (1987)
Abscisic acid promotes fusion of phospholipid vesicles
W. Stillwell (1991)
Do hydrophobic sequences cleaved from cellular polypeptides induce membrane fusion reactions in vivo?
J. Lucy (1984)
Fusion of phosphatidylcholine bilayer vesicles: role of free fatty acid.
H. Kantor (1978)
The fusogenic substance dimethyl sulfoxide enhances exocytosis in motor nerve endings.
N. Geron (1985)
Phenol-induced membrane changes in free and immobilized Escherichia coli
H. Keweloh (2004)
Fusion and Rupture of Lipid Model Membranes
Toon Stegmann (2001)
Anion effects on the structural organization of spinach thylakoid membranes
A. Jajoo (2006)
Divalent cation enhancement of the agglutinability by soyabean lectin of liposomes prepared from total lipid of erythrocytes and of erythrocyte membranes.
R. Rendi (1979)
Liposomes from ionic, single-chain amphiphiles.
W. Hargreaves (1978)
Reconstitution of Ion Channels in Planar Lipid Bilayers: New Approaches
J. Gaburjáková (2018)
Studies on the mechanism of membrane fusion: kinetics of calcium ion induced fusion of phosphatidylserine vesicles followed by a new assay for mixing of aqueous vesicle contents.
J. Wilschut (1980)
Abnormal aggregation behavior of acidic phospholipid vesicles in the very low concentration range of divalent cations
M. Shigematsu (1992)
Mast cell secretion: membrane events.
D. Lagunoff (1978)
Fractal aggregates induced by liposome-liposome interaction in the presence of Ca2+
J. Sabin (2007)
Physical properties and surface interactions of bilayer membranes containing N-methylated phosphatidylethanolamines.
J. Gagné (1985)See more